7 =JCM 1222] ;gi 1189500297 I ref I YP_001959767. 11 乙酰乳酸脫羧酶[褐桿狀綠菌 (Chlorobium phaeobacteroides)BSl] ;gi|189423787 I ref IΥΡ_001950964·?| 乙酰乳 酸脫羧酶[Geobacter lovleyi SZ] ;gi| 172058271| ref I ΥΡ_001814731· 1| 乙酰乳酸 脫羧酶[Exiguobacterium sibiricum 255_l5];gi|l63938775|ref|YP_001 643659.l 乙酰乳酸脫羧酶[魏登施泰滕芽孢桿菌（Bacillus weihenstephanensis)KBAB4]; gi|l58522304|ref|YP_001530174. l| 乙醜乳酸脫幾酶[Desulfococcus oleovorans Hxd3] ;gi|l57371670|ref|YP_001479659.l| 乙酰乳酸脫羧酶[變形斑沙雷菌（Serratia proteamaculans)568] ;gi|l5039511l|ref|YP_001317786.l| 乙醜乳酸脫幾酶[金黃色葡 萄球菌（Staphylococcus aureus)金黃亞種 JH1] ;gi| 150394715 I ref I YP_001317390. l| 乙 酰乳酸脫羧酶[金黃色葡萄球菌金黃亞種JH1] ;gi 1146311679 I ref I YP_001176753. 11乙酰 乳酸脫羧酶[腸桿菌屬菌種638] ;gi 1109900061 |ref I ΥΡ_663316· 11乙酰乳酸脫羧酶[大 西洋假別單胞菌（Pseudoalteromonas atlantica)T6c] ;gi|2l9866l3l|gb|ACL4647〇.l| 乙酰乳酸脫羧酶[藍(lán)桿藻物種PCC 7425] ;gi|21352455l|gb|ACJ53298.l|乙酰乳酸脫 羧酶[長(zhǎng)雙歧桿菌嬰兒亞種 ATCC 15697 = JCM 1222] ;gi|l89420046|gb|ACD94444.1 乙醜乳酸脫幾酶[Geobacter lovleyi SZ] ;gi| 158511130 I gb IABW68097. l| 乙醜乳 酸脫幾酶[Desulfococcus oleovorans Hxd3] ;gi| I57323434Igb I ABV4253LlI 乙 酰乳酸脫羧酶[變形斑沙雷菌568] ;gi 1145318555 I gb IABP60702. 11乙酰乳酸脫羧 酶[腸桿菌屬菌種.638] ;gi|l49947563|gb|ABR53499. 1|乙酰乳酸脫羧酶[金黃色 葡萄球菌金黃亞種JH1] ;gi|l49947167|gb|ABR53103. 1|乙酰乳酸脫羧酶[金黃色 葡萄球菌金黃亞種JH1] ;gi|l63860972|gb|ABY42031. 1|乙酰乳酸脫羧酶[魏登施 泰滕芽孢桿菌KBAB4] ;gi 1109702342 I gb IABG42262. 11乙酰乳酸脫羧酶[大西洋假 別單胞菌T6c] ;gi|l89495738|gb|ACE04286. 1|乙酰乳酸脫羧酶[褐桿狀綠菌BS1]; gi|l71990792|gb|ACB61714.l| 乙酰乳酸脫羧酶[Exiguobacterium sibiricum 255-15]; gi I 223932563 I ref I ΖΡ_03624564· 11 乙醜乳酸脫幾酶[豬鏈球菌菌（Streptococcus suis) 89/1591] ;gi 11944675311 ref I ZP_03073518. 11 乙酰乳酸脫羧酶[羅伊氏乳桿 菌（Lactobacillus reuteri)100_23] ;gi| 223898834 |gb I EEF65194. l| 乙酰乳酸脫羧 酶[豬鏈球菌菌89/1591] ;gi|l94454567|gb|EDX43464. 1|乙酰乳酸脫羧酶[羅伊氏乳 桿菌100-23] ;gi I 384267135 I ref I YP_005422842. 11乙酰乳酸脫羧酶[解淀粉芽孢桿 菌 plantarum 亞種 YAU B9601-Y2] ;gi|375364037|ref|YP_005132076.l| 乙酰乳酸脫羧 酶[解淀粉芽孢桿菌亞種 plantarum CAU B946] ;gi|34079323l|ref|YP_004758694.1 乙酰乳酸脫羧酶[可變棒狀桿菌（Corynebacterium variabile)DSM 44702]; gi| 336325119 I ref I ΥΡ_004605085· l| 乙酰乳酸脫羧酶[Corynebacterium resistens DSM 45100] ;gi|l48269032|ref|YP_001247975.l|乙酰乳酸脫羧酶[金黃色葡萄球菌金 黃亞種JH9] ;gi|l48268650|ref|YP_001247593.l|乙酰乳酸脫羧酶[金黃色葡萄球菌 金黃亞種JH9] ;gi|l48543372|ref|YP_001270742· 1|乙酰乳酸脫羧酶[羅伊氏乳桿菌 DSM20016] ;gi|380500488|emb|CCG51526. 1| 乙酰乳酸脫羧酶[解淀粉芽孢桿菌plantarum 亞種YAU B9601-Y2] ;gi I 371570031 |emb I CCF06881.ll乙酰乳酸脫羧酶[解淀粉芽孢桿 菌 plantarum 亞種 CAU B946] ;gi|34053314l|gb|AEK35621.l| 乙醜乳酸脫幾酶[可變棒 狀桿菌 DSM44702] ;gi |336101101 |gb|ΑΕΙ08921·1| 乙酰乳酸脫羧酶[Corynebacterium resistens DSM 45100] ;gi|l48530406|gb|ABQ82405.l| 乙酰乳酸脫羧酶[羅伊氏乳桿 菌DSM 20016] ;gi 1147742101 |gb| ABQ50399.11乙酰乳酸脫羧酶[金黃色葡萄球菌金黃 亞種JH9] ;gi|l47741719|gb|ABQ50017. 1|乙酰乳酸脫羧酶[金黃色葡萄球菌金黃亞種 JH9] ;gi I 392529510 I ref I ZP_10276647. 11 乙酰乳酸脫羧酶[肉梭狀桿菌（Carnobacterium maltaromaticum) ATCC 35586] ;gi| 366054074 I ref I ΖΡ_09451796· 1| 乙酰乳酸脫羧酶 [Lactobacillus suebicus KCTC 3M9] ;gi I 339624147 I ref I ΖΡ_〇8659936·11 乙酰乳酸脫 羧酶[Fructobacillus fructosus KCTC 3544] ;gi I 336393727 I ref I ΖΡ_08575126·11 乙酰 乳酸脫羧酶[棒狀乳桿菌（Lactobacillus coryniformis)扭曲亞種KCTC3535]。與之前的 編號(hào)有關(guān)的各序列以引用方式并入本文。
[0042] 醇脫氫酶（ADH)為催化醛和/或酮還原成醇的酶。仲醇脫氫酶對(duì)能夠制備仲醇的 底物特異性地具有催化活性。仲醇為其中與羥基（醇）官能團(tuán)結(jié)合的碳與2個(gè)其他的碳原 子共價(jià)結(jié)合的那些醇。根據(jù)特定的ADH，一些醇脫氫酶還可以催化相反的反應(yīng)，從而催化醇 向醛和/或酮的氧化。醇脫氫酶與它們的底物在酶的氧化還原反應(yīng)中通常使用含NAD的分 子（例如NAP+，NADH和NADPH)作為輔助因子。就本發(fā)明公開的方法而言，仲醇脫氫酶催化 醛和/或酮向醇的還原。
[0043] 醇脫氫酶催化屬于分類為EC I. I. I. 1 (醇脫氫酶活性）和基因本體（GO)語(yǔ)義ID 為G0:0004025的酶反應(yīng)。GO語(yǔ)義ID指定：表征為具有該相關(guān)GO語(yǔ)義的任何蛋白質(zhì)編碼 具有醇脫氫酶催化活性的酶。
[0044] 編碼醇脫氫酶的多種醇脫氫酶（adh)基因是本領(lǐng)域已知的。adh基因的實(shí)例 包含但不限于：gi I 223587866 I emb I CAX36647. 11醇脫氫酶[節(jié)細(xì)菌屬菌種JEK-2009]; gi| 343083017 I ref I ΥΡ_004772312· 1| 醇脫氫酶[Cyclobacterium marinum DSM 745]; gi|375I46863I ref IΥΡ_0〇5〇〇93〇4·?| 醇脫氫酶[Niastella koreensis GR2OlO]; gi|332296134|ref|YP_004438057.l| 醇脫氫酶[Thermodesulfobium narugense DSM H796] ;gi|3274〇3757|ref|YP_004344595· 1| 醇脫氫酶（NADP(+)) [Fluviicola taffensis DSM 16823] ;gi|325287739 I ref IΥΡ_004263529·1| 醇脫氫酶[Cellulophaga Iytica DSM 7489];gi|325295l43|ref|YP_0〇428l 657.l| 醇脫氫酶[Desulfurobacterium thermolithotrophumDSM ll699];gi|325289753|ref|YP_0〇4265934.l| 醇脫氫酶 [Syntrophobotulus glycolicus DSM 8271] ;gi I 325289499 I ref I ΥΡ_004265680·11 醇脫氫 酶[Syntrophobotulus glycolicus DSM 8271] ;gi|325110410|ref|YP_004271478.l| 醇 脫氫酶[Planctomyces brasiliensis DSM 5305] ;gi|325108594|ref|YP_004269662.1 醇脫氫酶[Planctomyces brasiliensis DSM 53O5] ;gi|3l995556〇|ref|YP_0〇4l 66827.l 醇脫氫酶[Cellulophaga algicola DSM14237] ;gi I 325065591 |gb I ADY73598.11 醇脫氫酶 [Desulfurobacterium thermolithotrophum DSM 11699] ；gi|332179237|gb|AEE14926. 1 醇脫氛酶[Thermodesulfobium narugense DSM 14796] ;gi|324970678|gb|ADY61456.l| 醇脫氛酶[Planctomyces brasiliensis DSM 5305] ;gi|324968862|gb|ADY59640.l| 醇 脫氛酶[Planctomyces brasiliensis DSM5305] ;gi|324965154|gb|ADY55933.l| 醇脫氛 酶[Syntrophobotulus glycolicus DSM 8271] ;gi|32496490〇|gb|ADY55679.l| 醇脫氫 酶[Syntrophobotulus glycolicus DSM 8271] ;gi|37437382l|ref|ZP_09631481.l| 醇脫 氫酶[Niabella soli DSM 19437];gi|373234794|gb|EHP54587.l| 醇脫氫酶[Niabella soli DSM 19437] ;gi|361060909|gb|AEV99900.l| 醇脫氫酶[Niastella koreensis GR20-10];gi|375l44658|ref|YP_0〇 5〇〇7〇99.l| 醇脫氫酶[Niastella koreensis GR20-10] ;gi I 332981006 I ref I ΥΡ_004462447· 11醇脫氫酶含鋅結(jié)合結(jié)構(gòu)域的蛋白質(zhì) [Mahella australiensis 50-1B0N] ;gi|332666040|ref|YP_004448828.l| 醇脫氫酶 [Haliscomenobacter hydrossis DSM 1100] ;gi|330837587|ref|YP_004412228.l| 醇脫 氫酶[Sphaerochaeta coccoides DSM I7374] ;gi I 33〇837463 I ref I YP_0〇44m〇4.l I 醇脫 氫酶[Sphaerochaeta coccoides DSM I7374] ;gi|33O8363I5 I ref IΥΡ_0〇441〇956·?| 醇 脫氫酶[Sphaerochaeta coccoides DSM I7374] ;giI 3274O55I4 I ref IΥΡ_0〇4346352· I 醇脫氫酶含鋅結(jié)合結(jié)構(gòu)域的蛋白質(zhì)[Fluviicola taffensis DSM 16823]; gi|325298359 I ref IΥΡ_004258276·1| 醇脫氫酶[Bacteroides salanitronis DSM 18170] ;gi|325291396|ref|YP_004267577.l| 醇脫氫酶[Syntrophobotulus glycolicus DSM 827l];gi|32528I283I ref IΥΡ_004253825·1| 醇脫氫酶（NADP(+))[Odoribacter splanchnicus DSM 20712] ;gi|325299524|ref|YP_004259441.l| 醇脫氫酶[Bacteroides salanitronis DSM I8I7O] ;gi|325l〇6236|ref|YP_00427589〇.l| 醇脫氫酶[Pedobacter saltans DSM m45] ;gi| 32O3333I5 I ref I ΥΡ_0〇417〇〇26·?| 醇脫氫酶[Deinococcus maricopensis DSM 2mi] ;gi|329749366 I gb I AEC〇2722· 1| 醇脫氫酶[Sphaerochaeta coccoides DSM17374];gi|324319077 IgbI ADY36968.ll 醇脫氫酶[Bacteroides salanitronis DSM 18170] ;gi|324317912 IgbI ADY35803.ll 醇脫氫酶[Bacteroides salanitronis DSM 18170] ;gi| 384129895 I ref I ΥΡ_005512508· l| 醇脫氫酶[嗜熱氫 桿菌（Hydrogenobacter thermophilus)TK_6] ;gi|3574l9〇ll|ref|YP_0〇4932〇〇3.l| 醇脫氫酶[Thermovirga lienii DSMl729I] ;gi|33298l〇68|ref|YP_0〇44625〇9· I 醇脫氫酶含鋅結(jié)合結(jié)構(gòu)域的蛋白質(zhì)[Mahella australiensis 50-1 BON]; gi|330837556 I ref IΥΡ_004412197·?| 醇脫氛酶[Sphaerochaeta coccoides DSM I7374] ;gi|33〇836856|ref|YP_0〇44ll497.l| 醇脫氛酶[Sphaerochaeta coccoides