專利名稱：新的肺炎衣原體表面蛋白的制作方法
技術(shù)領(lǐng)域：
本發(fā)明涉及人呼吸道病原體肺炎衣原體(Chlamydia pneumoniae)的、編碼大小約為89-101kDa和56-57kDa(較佳的約為89.6-100.3kDa和約為56.1kDa)的表面(surfaceexposed)膜蛋白的基因家族成員的鑒定。本發(fā)明涉及新的DNA序列，其對(duì)應(yīng)蛋白質(zhì)的推定的氨基酸序列，以及該DNA序列和蛋白在病理學(xué)和流行病學(xué)上診斷由肺炎衣原體引起的感染和作為疫苗組分的用途。
總體背景肺炎衣原體是專性胞內(nèi)細(xì)菌(Christiansen和Birkelund(1992)；Grayston等人，(1986))。它具有和革蘭陰性菌一樣的細(xì)胞壁結(jié)構(gòu)，具有外膜、周質(zhì)間隙和胞質(zhì)膜。用洗滌劑十二烷基肌氨酸鈉可以純化革蘭陰性菌的外膜。該組分被命名為“外膜復(fù)合物(OMC)”(Caldwell等人，(1981))。肺炎衣原體的COMC(衣原體外膜復(fù)合物)含有四組蛋白質(zhì)經(jīng)SDS-PAGE測(cè)得的98kDa高分子量蛋白、62/60kDa的富含半胱氨酸的外膜蛋白2(Omp2)的兩條條帶、38kDa的主要外膜蛋白(MOMP)、以及12kDa的低分子量脂蛋白Omp3。Omp2/Omp3和MOMP蛋白存在于所有衣原體種類(lèi)的COMC中，已經(jīng)從沙眼衣原體(C.trachomtis)、鸚鵡熱衣原體(C.psittaci)和肺炎衣原體中克隆出這些基因。然而編碼肺炎衣原體COMC中98kDa蛋白的基因還未被鑒別或克隆。
肺炎衣原體血清學(xué)和鑒定的現(xiàn)有狀況肺炎衣原體是一種專性胞內(nèi)細(xì)菌，屬于衣原體屬，該衣原體屬可以分為四種沙眼衣原體、肺炎衣原體、鸚鵡熱衣原體和C.pecorum。四個(gè)種類(lèi)的共同之處是它們?cè)诩?xì)胞內(nèi)的專性生長(zhǎng)，且它們具有胞外感染性顆粒(原體，EB)和胞內(nèi)復(fù)制形式(網(wǎng)狀體，RB)的雙相生命周期。另外，衣原體種類(lèi)的特征是在人類(lèi)感染中高度免疫原性的共同脂多糖(LPS)表位。沙眼衣原體引起人眼感染(沙眼)和生殖感染。鸚鵡熱衣原體是一類(lèi)可變的動(dòng)物病原體，其中的禽株偶爾會(huì)感染人并引起嚴(yán)重的肺炎鳥(niǎo)疫。第一個(gè)肺炎衣原體分離物從眼感染獲得，但是它被歸類(lèi)為非典型(non-typable)的衣原體。在芬蘭肺炎流行性爆發(fā)時(shí)，發(fā)現(xiàn)患者在衣原體屬特異性測(cè)試(淋巴肉芽腫皮膚試驗(yàn)抗原測(cè)試)中有陽(yáng)性反應(yīng)，且患者表現(xiàn)非典型的衣原體分離物的滴度升高。在西雅圖上呼吸道感染爆發(fā)時(shí)獲得了類(lèi)似的分離物，該衣原體分離物歸類(lèi)為一種新的種類(lèi)肺炎衣原體(Grayston等人，1989)。另外，有人指出肺炎衣原體涉及到粥樣動(dòng)脈硬化病變發(fā)展和刺激性支氣管哮喘(Kuo等人，1995)。這兩種病癥被認(rèn)為是由于慢性感染或過(guò)敏反應(yīng)或兩者共同引起的。
肺炎衣原體感染的診斷診斷肺炎衣原體引起的急性呼吸道感染是困難的。對(duì)患者樣品的肺炎衣原體培養(yǎng)是不敏感的，甚至是選擇合適的組織培養(yǎng)細(xì)胞未分離時(shí)也如此。因此Campbell等人(1992)開(kāi)發(fā)出一種肺炎衣原體特異性的聚合酶鏈反應(yīng)(PCR)。
盡管在一些研究中已經(jīng)用該P(yáng)CR方法檢測(cè)了肺炎衣原體，但是該方法是否適用于所有的臨床場(chǎng)合仍有爭(zhēng)論。其原因是，急性呼吸道感染中攜帶肺炎衣原體的細(xì)胞還未確定，且預(yù)計(jì)會(huì)有慢性攜帶狀態(tài)，只是不知道它們存在于哪些器官和細(xì)胞中。另外，PCR測(cè)試很難進(jìn)行，因?yàn)檫@些細(xì)菌的產(chǎn)量很低，且患者樣品中存在抑制性的物質(zhì)。因此，開(kāi)發(fā)出敏感的、特異性的血清型診斷方法來(lái)檢測(cè)急性和慢性感染是將會(huì)有很大價(jià)值。衣原體感染的血清學(xué)診斷方法目前是基于屬特異性的測(cè)試(如淋巴肉芽腫皮膚試驗(yàn)抗原測(cè)試和ELISA(測(cè)定LPS的抗體))，或是更特異性的種屬測(cè)試(用微量免疫熒光法(Micro-IF)測(cè)定針對(duì)純化的EB的抗體)(Wang等人，1970)。然而，微量免疫熒光法要用顯微鏡來(lái)讀數(shù)，且為了確保有正確的讀數(shù)，必須將結(jié)果與用作抗原(由于與共有的LPS表位的交叉反應(yīng)抗體)的沙眼衣原體的結(jié)果比較。因此，正如Kuo等人(1995)年所表述的，“本領(lǐng)域中主要需要一種用于臨床實(shí)驗(yàn)室的測(cè)試感染的迅速、可靠實(shí)驗(yàn)室方法”，本領(lǐng)域迫切需要開(kāi)發(fā)出用于針對(duì)肺炎衣原體的種特異性診斷方法。另外，肺炎衣原體可能涉及粥樣動(dòng)脈硬化和支氣管哮喘顯然也使得開(kāi)發(fā)有效的疫苗有正當(dāng)?shù)睦碛伞?br> 發(fā)明詳細(xì)公開(kāi)本發(fā)明的目的是提供有效診斷肺炎衣原體感染的方法以及開(kāi)發(fā)出有效抵抗該微生物感染的疫苗。因此，本發(fā)明涉及種特異性的哺乳動(dòng)物(如人)體內(nèi)肺炎衣原體感染的診斷測(cè)試方法，所述測(cè)試是根據(jù)對(duì)抗體進(jìn)行檢測(cè)，該抗體針對(duì)大小約為89-101kDa和56-57kDa(較佳的約為89.6-100.3kDa和56.1kDa)(推導(dǎo)的氨基酸序列的大小范圍為100.3至89.6，除大小為56.1的Omp13外)的表面膜蛋白，或是基于對(duì)編碼該蛋白或其變體或亞序列的核酸片段進(jìn)行檢測(cè)。本發(fā)明還涉及本發(fā)明蛋白的氨基酸序列，其變體和亞序列，以及編碼這些蛋白或其變體或亞序列的核酸片段。本發(fā)明還涉及針對(duì)本發(fā)明蛋白的抗體。本發(fā)明還涉及本發(fā)明的核酸片段和蛋白在診斷肺炎衣原體以及抗肺炎衣原體疫苗中的用途。
在本發(fā)明公開(kāi)前，只有很少的肺炎衣原體基因被測(cè)序。它們主要是編碼已知的沙眼衣原體同系物(MOMP、Omp2、Omp3、Kdo轉(zhuǎn)移酶)的基因、熱休克蛋白基因GroEl/Es和Dnak(一種核酸酶P同系物)和編碼功能未知的76kDa蛋白的基因。為何至今只有如此少的基因被克隆的原因是因?yàn)閺乃拗骷?xì)胞純化后能獲得的肺炎衣原體的產(chǎn)量非常低。在這種純化后，DNA必須從EB中純化出，而在此步驟肺炎衣原體DNA容易被宿主細(xì)胞DNA污染。除了這些固有的困難外，培育肺炎衣原體并用DNA技術(shù)產(chǎn)生很少數(shù)量(幾個(gè)μ)DNA的表達(dá)文庫(kù)非常困難。自1993年起已經(jīng)知道(Melgosa等人，1993)，98kDa的蛋白存在于肺炎衣原體的OMC中。盡管Melgosa提到98kDa的蛋白條帶是肺炎衣原體OMC的一部分，但是其基因序列以及推導(dǎo)的氨基酸序列一直未被確定。該文中只描述了用SDS-PAGE大致分離出的肺炎衣原體蛋白的條帶。然而，在本發(fā)明前，編碼該蛋白的基因一直未被確定。發(fā)現(xiàn)98kDa蛋白與人血清只有很弱的反應(yīng)或沒(méi)有反應(yīng)(Campbell等人，1990)，在本發(fā)明者的工作之前，還沒(méi)有認(rèn)識(shí)到89-101kDa蛋白是表面外露的或它們實(shí)際上有免疫原性。在該報(bào)道中描述了許多人血清樣品能與SDS-PAGE中遷移至98kDa的肺炎衣原體蛋白反應(yīng)。該蛋白沒(méi)有進(jìn)一步作特性分析，因此它與本申請(qǐng)沒(méi)有沖突。
Halme等人1997年描述了92-98kDa肺炎衣原體蛋白中存在人T細(xì)胞表位。從總衣原體蛋白的SDS-PAGE中洗脫出該蛋白，但是該蛋白的身份沒(méi)有被確定。
用抗體來(lái)篩選表達(dá)文庫(kù)是克隆編碼蛋白的抗原性部分基因片段眾所周知的方法。然而，由于患者的血清沒(méi)有表現(xiàn)出與98kDa蛋白有明顯的反應(yīng)，因此不可能用患者血清來(lái)克隆該蛋白。
已知由發(fā)明者獲得的單克隆抗體能與肺炎衣原體表面上的構(gòu)象表位反應(yīng)，它們還與肺炎衣原體OMC反應(yīng)(經(jīng)免疫電子顯微鏡測(cè)得)(Christiansen等人，1994)。另外，98kDa蛋白是肺炎衣原體OMC中唯一未知的蛋白(Melgosa等人，1993)。本發(fā)明者選擇采用非常規(guī)的步驟來(lái)克隆編碼至今未知的98kDa蛋白的基因純化肺炎衣原體OMC，在免疫接種前用SDS處理抗原破壞免疫原性構(gòu)象表位。從而獲得對(duì)免疫原性較低的線性表位的抗體(PAB150)。這提供了獲得能檢測(cè)該蛋白的抗血清的可能性，并表明編碼本發(fā)明的89-101kDa和56kDa蛋白的基因家族能在重組體大腸桿菌的菌落印跡中檢測(cè)到。
肺炎衣原體感染的小鼠產(chǎn)生了針對(duì)發(fā)明者鑒定并命名為Omp4-15的抗體，但是不識(shí)別通常用于SDS-PAGE和免疫印跡的經(jīng)SDS處理熱變性的抗原。但是，如果抗原沒(méi)有熱變性，則可見(jiàn)到強(qiáng)反應(yīng)。因此，如果在人感染中見(jiàn)到類(lèi)似的反應(yīng)，則本發(fā)明的抗原很有可能在血清診斷測(cè)試中具有不可估量的用途，并且很可能用作預(yù)防感染的疫苗。
通過(guò)產(chǎn)生針對(duì)肺炎衣原體COMC的抗體，獲得與所有蛋白反應(yīng)的多克隆抗體(PAB150)。用該抗體鑒別肺炎衣原體DNA表達(dá)文庫(kù)中編碼89.6-101.3kDa和56.1kDa的基因。與本發(fā)明有關(guān)的一個(gè)問(wèn)題是在肺炎衣原體中發(fā)現(xiàn)了包含許多類(lèi)似基因的一個(gè)家族。因此，需要大量不同的克隆來(lái)鑒別諸片段簇。本發(fā)明者成功克隆并測(cè)序以上四個(gè)基因只是因?yàn)橛肧DS-變性抗原產(chǎn)生的家兔抗體含有針對(duì)位于該蛋白家族不同成員中的許多不同表位的抗體。通過(guò)該方法，一個(gè)基因已被完全測(cè)序，第二個(gè)基因除遠(yuǎn)部外已測(cè)序，并獲得了兩個(gè)附加基因的更短的片段。為了獲得附加基因的DNA序列并搜尋該基因家族的更多成員，用測(cè)序基因衍生的引物和數(shù)據(jù)庫(kù)中已經(jīng)公開(kāi)基因的引物進(jìn)行長(zhǎng)范圍(long range)PCR。該方法檢測(cè)到屬于該家族的另外8個(gè)基因。這些基因位于兩個(gè)基因簇中Omp12，11，10，5，4，13和14在一個(gè)簇中，Omp6，7，8，9和15在第二個(gè)簇中。從Omp4，5，6，7，8，9，10，11和13獲得全序列，并獲得Omp12，14部分序列。Omp13是1545核苷酸的截短的基因，全長(zhǎng)基因的其余部分為2526(Omp7)至2838(Omp15)的核苷酸。推導(dǎo)的氨基酸序列揭示，推定的多肽為89.6至100.3kDa，除Omp13為56.1kDa外。與所有序列相比，推導(dǎo)的氨基酸序列的排列表現(xiàn)出最大相同性為49％(Omp5/Omp9)。除了Omp13外，最低的同源性是Omp7與其它任何氨基酸序列的相同性不到34％。Omp13與所有其它序列的同源性評(píng)分為29-32％。
在本文中，SEQ ID NO1和2對(duì)應(yīng)于Omp4，SEQ ID NO3和4對(duì)應(yīng)于Omp5，SEQ ID NO5和6對(duì)應(yīng)于Omp6，SEQ ID NO7和8對(duì)應(yīng)于Omp7，SEQ ID NO9和10對(duì)應(yīng)于Omp8，SEQ ID NO11和12對(duì)應(yīng)于Omp9，SEQ ID NO13和14對(duì)應(yīng)于Omp10，SEQ ID NO15和16對(duì)應(yīng)于Omp11，SEQ ID NO17和18對(duì)應(yīng)于Omp12，SEQ ID NO19和20對(duì)應(yīng)于Omp13，SEQ ID NO21和22對(duì)應(yīng)于Omp14，SEQ ID NO23和24對(duì)應(yīng)于Omp15。
下面列出了本發(fā)明的Omp蛋白估計(jì)的大小。Omp4的大小為98.9kDa，Omp5的估計(jì)大小為97.2kDa，Omp6的估計(jì)大小為100.3kDa，Omp7的估計(jì)大小為89.7kDa，Omp8的估計(jì)大小為90.0kDa，Omp9的估計(jì)大小為96.7kDa，Omp10的估計(jì)大小為98.4kDa，Omp11的估計(jì)大小為97.6kDa，Omp13的估計(jì)大小為56.1kDa，Omp12和Omp14為一部分。
另外，SEQ ID NO25是SEQ ID NO3的亞序列，SEQ ID NO26是SEQ ID NO4的亞序列，SEQ ID NO27是SEQ ID NO5的亞序列，SEQ ID NO28是SEQ ID NO6的亞序列，SEQ ID NO29是SEQ ID NO7的亞序列，SEQ ID NO30是SEQ ID NO8的亞序列。
部分omp蛋白以融合蛋白形式表達(dá)并產(chǎn)生了針對(duì)這些蛋白的小鼠多克隆單特異性抗體。這些抗體在免疫熒光和免疫電鏡法中與肺炎衣原體的表面反應(yīng)。這第一次表明肺炎衣原體中89-101kDa和56-57kDa蛋白家族包含表面的外膜蛋白。這一重要發(fā)現(xiàn)導(dǎo)致這樣一個(gè)認(rèn)識(shí)，即89-101kDa和56-57kDa肺炎衣原體蛋白家族成員是開(kāi)發(fā)血清學(xué)診斷測(cè)試肺炎衣原體、以及開(kāi)發(fā)抵抗肺炎衣原體感染的疫苗(根據(jù)采用這些蛋白)的優(yōu)秀的候選物。另外，這些蛋白可用作流行病學(xué)標(biāo)記，針對(duì)這些蛋白的多克隆單特異性血清可用來(lái)檢測(cè)人組織中的肺炎衣原體或用來(lái)檢測(cè)組織培養(yǎng)物中的肺炎衣原體分離物。另外，編碼89-101kDa和56-57kDa(如89.6-100.3kDa和56.1蛋白家族)的基因可用來(lái)開(kāi)發(fā)基于核酸檢測(cè)/擴(kuò)增的種特異性診斷測(cè)試。
將全長(zhǎng)Omp14克隆到允許Omp4多肽表達(dá)的表達(dá)載體系統(tǒng)中。用該多肽作為抗原來(lái)免疫家兔。由于該蛋白在變性條件下純化，因此其抗體不與肺炎衣原體天然表面反應(yīng)，但是在采用純化的肺炎衣原體EB作為抗原的免疫印跡中，它和98kDa蛋白反應(yīng)。另外，抗體在試驗(yàn)性感染小鼠的石蠟包埋肺組織切片中反應(yīng)。
本發(fā)明較寬的方面涉及種特異性地診斷測(cè)試肺炎衣原體感染哺乳動(dòng)物(如人)的方法，所述測(cè)試方法包括檢測(cè)患者體內(nèi)或較佳的是患者樣品中是否存在針對(duì)分子量為89-101kDa或56-57kDa的肺炎衣原體外膜蛋白的抗體，或檢測(cè)是否存在編碼所述外膜蛋白或其片段的核酸片段。
在本申請(qǐng)的內(nèi)容中，術(shù)語(yǔ)“患者樣品”應(yīng)指患者(如人患者)的一定量的血清、血漿、粘液、組織、咳出物、強(qiáng)迫咳出的痰液或支氣管吸出物、尿液、腦脊髓液、粥樣動(dòng)脈硬化病灶、粘膜拭子(mucosal swap)、來(lái)自所述患者的組織培養(yǎng)的細(xì)胞、或以任何方式源自所述患者的物質(zhì)。本發(fā)明的人體內(nèi)測(cè)試包括本領(lǐng)域中已知的皮膚測(cè)試，如真皮內(nèi)測(cè)試，例如類(lèi)似于Mantaux測(cè)試。在對(duì)于測(cè)試非常敏感的某些患者(通常情況是兒童)體內(nèi)，測(cè)試可以是非侵入型的，例如用膏藥在皮膚表面測(cè)試。
在本文中，術(shù)語(yǔ)89-101kDa蛋白指通常在肺炎衣原體外膜中的蛋白，其在SDS-PAGE中所見(jiàn)的一條或多條條帶，表觀分子量基本上在89-101kDa范圍內(nèi)。根據(jù)推導(dǎo)的氨基酸序列，分子大小在89.6-100.3kDa之間不等。
基于利用屬于本申請(qǐng)書(shū)公開(kāi)的基因家族的基因的種特異性血清診斷測(cè)試在本發(fā)明的范圍內(nèi)。
本發(fā)明的較佳實(shí)例涉及本發(fā)明的種特異性診斷測(cè)試，其中外膜蛋白具有選自SEQID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24的序列。
當(dāng)采用本發(fā)明的蛋白時(shí)，術(shù)語(yǔ)“變體”應(yīng)理解成表現(xiàn)出與本發(fā)明的某一種蛋白的相似性低于100％的氨基酸序列。變體序列的大小可以和與之比較的序列相同或不同。變體通常宜表現(xiàn)出序列相似性至少為50％，較佳的至少60％，更佳的至少70％，例如至少80％、90％、95％或98％。
關(guān)于本發(fā)明蛋白序列的術(shù)語(yǔ)“序列相似性”指本發(fā)明蛋白和長(zhǎng)度相同或不同的對(duì)比蛋白中相同的和保守性變化的氨基酸殘基(在位置和類(lèi)型上)的百分?jǐn)?shù)。關(guān)于本發(fā)明蛋白序列的術(shù)語(yǔ)“序列相同性”指本發(fā)明蛋白和長(zhǎng)度相同或不同的對(duì)比蛋白中在位置和類(lèi)型上相同的氨基酸的百分?jǐn)?shù)。
在本發(fā)明的范圍內(nèi)，本發(fā)明的一種蛋白的亞序列是指取自SEQ ID NO2、SEQ IDNO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24的氨基酸殘基的連續(xù)序列。一個(gè)亞序列通常包含至少100個(gè)氨基酸，較佳的至少80個(gè)氨基酸，更佳的至少70個(gè)氨基酸，如50個(gè)氨基酸。它甚至可以短達(dá)10-50個(gè)氨基酸，例如20-40個(gè)氨基酸，例如約30個(gè)氨基酸。亞序列通常表現(xiàn)出的序列同源性至少為50％，較佳的至少為60％，更佳的至少70％，例如至少80％，如至少90％，95％或98％。
本發(fā)明的診斷測(cè)試所包括的免疫試驗(yàn)選自直接或間接的EIA(如ELISA)、免疫印跡技術(shù)(如Western印跡)、放射免疫試驗(yàn)和其它任何非酶聯(lián)抗體結(jié)合試驗(yàn)或諸如熒光、凝集或沉淀反應(yīng)的程序，以及比濁法。
本發(fā)明的一個(gè)較佳實(shí)例涉及本發(fā)明的種特異性診斷測(cè)試，所述測(cè)試包括ELISA，其中檢測(cè)樣品中針對(duì)本發(fā)明蛋白或其片段的抗體。
本發(fā)明的一個(gè)較佳的實(shí)例是以檢測(cè)樣品中針對(duì)本發(fā)明蛋白的抗體為基礎(chǔ)的ELISA。該ELISA可采用本發(fā)明的蛋白或其變體(即抗原)作為包被試劑。ELISA通常根據(jù)本領(lǐng)域熟知的標(biāo)準(zhǔn)方法(例如David Lane Harlow編輯的“抗體實(shí)驗(yàn)手冊(cè)”(ColdSpring Habor laboratories，1988)中所述的方法，該文納入本文作參考)來(lái)進(jìn)行。
重組蛋白可用按照下文實(shí)施例中所述的方法獲得的DNA序列來(lái)生產(chǎn)。這些DNA序列(包括本發(fā)明基因家族中每個(gè)基因的整個(gè)編碼區(qū))將被克隆到表達(dá)載體中，從該表達(dá)載體中可以純化獲得推導(dǎo)的蛋白序列。在ELISA中用單克隆和多克隆抗體以及實(shí)驗(yàn)性感染的小鼠的血清和人患者的血清分析純化獲得的蛋白的反應(yīng)性。
從實(shí)驗(yàn)性感染的小鼠血清的反應(yīng)性知道，它主要識(shí)別的是非線性表位。因此，可以考慮用本領(lǐng)域中已知的不同形式的純化方案來(lái)分析不連續(xù)表位的存在，和分析人免疫應(yīng)答是否也針對(duì)這類(lèi)表位。
本發(fā)明的較佳實(shí)例涉及本發(fā)明的種特異性診斷測(cè)試，其中核酸片段具有選自SEQID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21和SEQ ID NO23的序列。
關(guān)于本發(fā)明的核酸片段，術(shù)語(yǔ)“變體”應(yīng)理解成表現(xiàn)出的序列同源性低于100％的核酸序列。變體序列的大小可以和與之比較的序列相同或不同。變體通常宜表現(xiàn)出序列同源性至少為50％，較佳的至少60％，更佳的至少70％，例如至少80％、90％、95％或98％。
關(guān)于本發(fā)明的核酸片段，術(shù)語(yǔ)“序列同源性”指本發(fā)明的核酸片段中的核酸在位置和類(lèi)型上與長(zhǎng)度相同或不同的序列對(duì)比時(shí)相匹配核酸百分?jǐn)?shù)。
為了獲得關(guān)于本發(fā)明每個(gè)基因總體分布的信息，對(duì)于所有可獲得的肺炎衣原體分離物上的每個(gè)基因進(jìn)行PCR。這將提供關(guān)于本發(fā)明的基因或核酸片段的總體變化性的信息。對(duì)可變區(qū)測(cè)序?？捎肞CR從患者樣品擴(kuò)增出流行病學(xué)基因的可變部分。不變的部分可用來(lái)PCR擴(kuò)增和分析用于診斷測(cè)試的可能性。預(yù)計(jì)，如果發(fā)現(xiàn)可變性，則可變區(qū)的PCR可用于流行病學(xué)。不變區(qū)的PCR可用作種特異性診斷測(cè)試。用編碼所有已知分離物中不變蛋白的基因作為PCR的靶來(lái)制備編碼功能未知蛋白的基因。
本發(fā)明的特別佳的實(shí)例涉及本發(fā)明的診斷測(cè)試，其中利用核酸擴(kuò)增、較佳的是聚合酶鏈反應(yīng)(PCR)來(lái)檢測(cè)核酸片段。
以PCR為基礎(chǔ)的測(cè)試在本發(fā)明的范圍內(nèi)，該測(cè)試的目的是檢測(cè)本發(fā)明的核酸片段或其變體。PCR測(cè)試通常根據(jù)本領(lǐng)域熟知的方法來(lái)進(jìn)行，其通常包括能檢測(cè)和區(qū)別本發(fā)明的核酸片段的PCR測(cè)試。較佳的是定量的競(jìng)爭(zhēng)性PCR測(cè)試或嵌套式PCR測(cè)試。本發(fā)明的PCR測(cè)試通常根據(jù)EPB540588、EPA586112、EPA643140或EPA669401中詳細(xì)描述的方法來(lái)設(shè)計(jì)，這些專利均納入本文作參考。
在本發(fā)明的范圍內(nèi)，本發(fā)明的一種核酸片段的變體和亞序列指取自SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21和SEQ ID NO23的連續(xù)延伸核酸。變體或亞序列宜包含至少100個(gè)核酸，較佳的至少80個(gè)核酸，更佳的至少70個(gè)核酸，例如至少50個(gè)核酸。它甚至可以短達(dá)10-50個(gè)核酸，例如20-40個(gè)核酸，例如約30個(gè)核酸。亞序列通常表現(xiàn)出至少30％的序列同源性，較佳的為至少60％，更佳的為至少70％，例如至少80％，如至少90％、95％或98％。亞序列越短，所需的同源性越高。因此，100個(gè)核酸或更少的亞序列必須表現(xiàn)出至少80％的同源性。
本發(fā)明的一個(gè)非常重要的方面涉及本發(fā)明衍生自肺炎衣原體的蛋白，該蛋白具有的氨基酸序列選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ IDNO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24，具有的序列相似性至少為50％，較佳的至少60％，更佳的至少70％，例如至少80％，如至少90％、95％或98％，并具有類(lèi)似的生物功能。
術(shù)語(yǔ)“類(lèi)似的生物功能”指該蛋白表現(xiàn)出類(lèi)似于可從肺炎衣原體膜蛋白衍生獲得的蛋白的性質(zhì)特征。這些蛋白包括GGAI的重復(fù)基序(至少2個(gè)，較佳的至少3個(gè)重復(fù)序列)和/或保守的色氨酸(w)位置。
比較編碼Omp4-15的基因的DNA序列，結(jié)果表明，單個(gè)基因之間的總體相似性范圍在43-55％之間。比較Omp4-15的氨基酸序列結(jié)果表明相同性為34-49％，相似性為53-64％。同源性一般沿推導(dǎo)的氨基酸整個(gè)長(zhǎng)度分散散布。然而，從圖8A-J看出，存在一些區(qū)域其同源性更明確。這見(jiàn)于重復(fù)序列中，其中序列GGAI在基因中重復(fù)4-7次。令人感興趣的是，對(duì)于編碼四個(gè)氨基酸GGAI的序列，DNA同源性并不保守。這可能表明了蛋白該部分的功能性作用，并表明該重復(fù)結(jié)構(gòu)并不是由基因復(fù)制而產(chǎn)生的。除了四個(gè)氨基酸的重復(fù)序列GGAI外，氨基酸400-490的區(qū)域所具有的同源性要高于該蛋白的其它部分，在所有序列中均有保守序列FYDPI。功能上相似性進(jìn)一步表明，氨基酸色氨酸(W)在該蛋白C端部分4-6個(gè)局部區(qū)域完全保守。
由于本發(fā)明的基因和推導(dǎo)的氨基酸序列沒(méi)有一個(gè)相同，因此下列內(nèi)容在本發(fā)明的范圍內(nèi)單特異性抗體的生產(chǎn)，用所述抗體來(lái)鑒別哪個(gè)肺炎衣原體蛋白被表達(dá)，用所述抗體鑒別所述肺炎衣原體蛋白在發(fā)育生命周期的何時(shí)被表達(dá)，用所述抗體鑒定所述肺炎衣原體蛋白的精確的細(xì)胞定位。用針對(duì)本發(fā)明蛋白的單特異性抗體來(lái)測(cè)定所述蛋白的哪個(gè)部分是外露的，肺炎衣原體COMC中的蛋白是怎樣相互作用的，這些也在本發(fā)明的范圍內(nèi)。
本發(fā)明較佳的實(shí)例涉及包含本發(fā)明蛋白質(zhì)亞序列的多肽，所述亞序列包含序列GGAI。本發(fā)明其它較佳的實(shí)例涉及包含本發(fā)明蛋白亞序列的多肽，所述亞序列包含序列FSGE。
本發(fā)明的多肽的長(zhǎng)度通常為至少6個(gè)氨基酸，較佳的至少15個(gè)氨基酸，較佳的至少20個(gè)氨基酸，較佳的至少25個(gè)氨基酸，較佳的至少30個(gè)氨基酸，較佳的至少35個(gè)氨基酸，較佳的至少40個(gè)氨基酸，較佳的至少45個(gè)氨基酸，較佳的至少50個(gè)氨基酸，較佳的至少55個(gè)氨基酸，較佳的至少100個(gè)氨基酸。
本發(fā)明非常重要的一方面涉及本發(fā)明衍生自肺炎衣原體的核酸片段，其變體和亞序列。
本發(fā)明另一個(gè)重要的方面涉及針對(duì)本發(fā)明蛋白的抗體，這些抗體包括針對(duì)具有選自序列SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24的蛋白的多克隆單特異性抗體和單克隆抗體。
本發(fā)明的一個(gè)非常重要的方面涉及用來(lái)診斷哺乳動(dòng)物(如人)感染肺炎衣原體的診斷試劑盒，所述試劑盒包含的一種或多種蛋白具有選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24的氨基酸序列。
本發(fā)明另一非常重要的方面涉及用來(lái)診斷哺乳動(dòng)物(如人)感染肺炎衣原體的診斷試劑盒，所述試劑盒包含針對(duì)具有選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24的氨基酸序列的蛋白的抗體。本發(fā)明的診斷試劑盒中包括的抗體可以是多克隆抗體或單克隆抗體或其混合物。
本發(fā)明還有一個(gè)重要的方面涉及用來(lái)診斷哺乳動(dòng)物(如人)感染肺炎衣原體的診斷試劑盒，所述試劑盒包含具有選自SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQID NO17、SEQ ID NO19、SEQ ID NO21和SEQ ID NO23的序列的一種或多種核酸片段。
本發(fā)明一方面涉及一種用于免疫接種哺乳動(dòng)物(如人)抵抗肺炎衣原體的組合物，所述組合物包含具有選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ IDNO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24的氨基酸序列的一種或多種蛋白質(zhì)。
預(yù)計(jì)本發(fā)明的蛋白在預(yù)防哺乳動(dòng)物(如人)感染肺炎衣原體上有重要作用。因此，通常用重組技術(shù)產(chǎn)生本發(fā)明的蛋白(包括其變體和亞序列)，然后用作免疫接種哺乳動(dòng)物(如家兔)的抗原。隨后，用組織培養(yǎng)試驗(yàn)分析免疫獲得的超級(jí)免疫血清抵抗肺炎衣原體的保護(hù)作用。另外，可以考慮生產(chǎn)單克隆抗體(通常用標(biāo)準(zhǔn)的雜交瘤技術(shù))，并分析其抵抗肺炎衣原體感染的保護(hù)作用。
預(yù)計(jì)在本發(fā)明的蛋白(包括所述蛋白的亞序列)中可以找到特別感興趣的和免疫原性表位。較佳的是用包含本發(fā)明蛋白的這些亞序列的多肽來(lái)免疫哺乳動(dòng)物(如人)以抵抗肺炎衣原體。
本發(fā)明的一個(gè)重要的方面涉及下述蛋白在診斷哺乳動(dòng)物(如人)受肺炎衣原體感染中的應(yīng)用，該蛋白具有的序列選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24。
本發(fā)明的一個(gè)較佳的實(shí)例涉及非變性形式的本發(fā)明蛋白在診斷哺乳動(dòng)物(如人)受肺炎衣原體感染中的應(yīng)用。
本發(fā)明的一個(gè)非常重要的方面涉及下述蛋白在免疫哺乳動(dòng)物(如人)抵抗肺炎衣原體中的應(yīng)用，該蛋白具有的序列選自SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22和SEQ ID NO24。
本發(fā)明的一個(gè)較佳的實(shí)例涉及非變性形式的本發(fā)明蛋白在免疫哺乳動(dòng)物(如人)抵抗肺炎衣原體中的應(yīng)用。
本發(fā)明的一個(gè)非常重要的方面涉及下述核酸片段在免疫哺乳動(dòng)物(如人)抵抗肺炎衣原體中的應(yīng)用，該核酸片段具有的核苷酸序列選自SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21和SEQ ID NO23。
預(yù)計(jì)可以開(kāi)發(fā)出一類(lèi)用基因槍接種小鼠抗肺炎衣原體的疫苗。通常，含有本發(fā)明的核酸片段、核酸片段組合的不同基因構(gòu)建物可用于基因槍方法。隨后分析小鼠產(chǎn)生的體液和細(xì)胞免疫應(yīng)答以及在攻擊后其抵抗肺炎衣原體感染的保護(hù)作用。
鑒于此，本發(fā)明還涉及本發(fā)明蛋白作為藥物(疫苗)的用途以及用來(lái)制備抵抗肺炎衣原體感染的疫苗的用途。
含有蛋白序列作為活性組分的疫苗的制備方法是本領(lǐng)域中所熟知的，例子有美國(guó)專利4,608,251；4,601,903；4,599,231；4,599,230；4,596,792和4,578,770，所有這些文獻(xiàn)均納入本文作參考。通常，這些疫苗被制成液體溶液或懸浮液形式的可注射劑；還可制成適合在注射液體前溶于溶液或懸浮液的固體形式。制劑還可乳化?；钚悦庖呓M分通常和藥學(xué)上可接受的并與活性組分相容的賦形劑混合。合適的賦形劑例如是水、鹽水、葡萄糖、甘油、乙醇等以及它們的組合。另外，如果需要，疫苗還可含有少量輔助物質(zhì)如潤(rùn)濕劑或乳化劑、pH緩沖劑、或增強(qiáng)疫苗效果的佐劑。
疫苗通常是經(jīng)腸胃外注射給予，例如皮下注射或肌內(nèi)注射。適用于其它給藥方式的其它制劑包括栓劑以及在一些場(chǎng)合下的口服制劑。這些組合物的形式是溶液、懸浮液、片劑、丸劑、膠囊劑、緩釋制劑或粉末，含有10-95％(較佳的為25-70％)的活性組分，并任選地含有合適的載體。
蛋白序列可以本領(lǐng)域已知的中性或鹽形式配制入疫苗內(nèi)。疫苗以相容于劑型的方式給予，其量在治療上是有效的并且有免疫原性。給藥量取決于待治療的對(duì)象。合適的劑量范圍為每次接種約幾百微克活性組分，較佳的范圍約為0.1-1000微克。如果疫苗還包含本領(lǐng)域已知的佐劑物質(zhì)，則可增強(qiáng)免疫應(yīng)答。其它可能性包括采用免疫調(diào)節(jié)物質(zhì)如淋巴因子(如IFN-γ、IL-2和IL-12)或合成的IFN-γ誘導(dǎo)劑如與上述佐劑組合的聚I∶C。
通過(guò)下述方式還可產(chǎn)生活疫苗將編碼本發(fā)明的蛋白片段或蛋白的至少一個(gè)核酸片段導(dǎo)入非病原性微生物中，使蛋白片段或蛋白在微生物表面表達(dá)(例如包括膜錨著部分的融合蛋白形式或攜帶允許錨著到膜上的脂質(zhì)化(lipidation)信號(hào)的略經(jīng)修飾的蛋白或蛋白片段形式)。技術(shù)人員知道怎樣使相關(guān)表達(dá)系統(tǒng)適合此目的。
本發(fā)明另一部分依據(jù)的事實(shí)是，最近的研究揭示，克隆到在載體中的在真核細(xì)胞內(nèi)的非復(fù)制性的DNA片段可通過(guò)(例如)肌內(nèi)注射或經(jīng)皮膚給藥(所謂的“基因槍”方法)導(dǎo)入動(dòng)物(包括人)體內(nèi)。DNA被肌細(xì)胞攝取，感興趣的基因被在真核細(xì)胞內(nèi)起作用的啟動(dòng)子(例如病毒啟動(dòng)子)表達(dá)，隨后基因產(chǎn)物刺激免疫系統(tǒng)。Ulmer等人在1993年回顧了這些新發(fā)現(xiàn)的方法，該文納入本文作參考。
因此，編碼本發(fā)明蛋白的核酸片段可用來(lái)實(shí)現(xiàn)體內(nèi)表達(dá)抗原，即核酸片段可用作所謂的DNA疫苗。因此，本發(fā)明還涉及一種疫苗，它包含編碼本發(fā)明的蛋白片段或蛋白的核酸片段，該疫苗使已給予疫苗的哺乳動(dòng)物(如人)在體內(nèi)表達(dá)抗原，表達(dá)的抗原量能有效地使哺乳動(dòng)物(如人)對(duì)體內(nèi)肺炎衣原體感染的抵抗力大大增加。
通過(guò)將編碼表達(dá)產(chǎn)物的基因和編碼能調(diào)節(jié)免疫應(yīng)答的蛋白的DNA片段一起給予，可以增強(qiáng)這種“DNA疫苗”的效率。例如，通過(guò)給予兩種分開(kāi)的DNA片段或給予包含在同一載體中的兩種DNA片段，編碼淋巴因子前體或淋巴因子(例如IFN-γ、IL-2或IL-12)的基因可以和編碼免疫原性蛋白片段或蛋白的基因一同給予。還有一個(gè)可能性是給予包含多個(gè)核苷酸序列的DNA片段，其中每個(gè)核苷酸序列編碼本文公開(kāi)的蛋白片段和蛋白的相對(duì)表位，用這些表位的寬譜來(lái)實(shí)現(xiàn)連續(xù)致敏免疫應(yīng)答。
下列實(shí)驗(yàn)為非限制性實(shí)施例，目的是為了描述本發(fā)明的某些特征和實(shí)例。
附例

圖1顯示了負(fù)染色的純化肺炎衣原體EB(A)和純化的OMC(B)的電鏡結(jié)果。
圖2顯示了純化的EB和OMC銀染的15％SDS-PAGE結(jié)果。泳道1，純化的肺炎衣原體EB；泳道2，肺炎衣原體OMC；泳道3，純化的沙眼衣原體EB；和泳道4，沙眼衣原體OMC。
圖3顯示了經(jīng)10％SDS-PAGE分離、轉(zhuǎn)移到硝酸纖維素膜并和家兔抗肺炎衣原體OME血清反應(yīng)的免疫印跡。
圖4顯示了經(jīng)家兔抗肺炎衣原體血清檢測(cè)的重組pEX的考馬斯藍(lán)染色7.5％SDS-PAGE結(jié)果。箭頭表示117kDaβ半乳糖苷酶蛋白的定位。
圖5顯示了經(jīng)菌落印跡檢測(cè)、7.5％SDS-PAGE分離、轉(zhuǎn)移到硝酸纖維素膜上并和家兔抗肺炎衣原體OMC反應(yīng)的重組體pEX克隆的免疫印跡。泳道1，海藍(lán)色(seablue)分子量標(biāo)準(zhǔn)。泳道2-6，于42℃培育以誘導(dǎo)產(chǎn)生β半乳糖苷酶融合蛋白的pEX克隆。
圖6顯示了Omp4和Omp5的序列策略。箭頭表示用來(lái)測(cè)序的引物。
圖7。肺炎衣原體omp基因?；蚺帕性趦蓚€(gè)簇中。在第1簇中發(fā)現(xiàn)Omp12、11、10、5、4、13和14。在第2簇中發(fā)現(xiàn)Omp6、7、8、9和15。
圖8A-J顯示了肺炎衣原體4-15的序列對(duì)比，采用GCG軟件包中的pile up程序。
圖9顯示了肺炎衣原體感染的HeLa細(xì)胞感染后72小時(shí)與針對(duì)pEX3-36融合蛋白的小鼠單特異性抗血清反應(yīng)的免疫熒光。pEX3-36是Omp5基因的一部分。
圖10顯示了肺炎衣原體EB的免疫印跡。泳道1-3，在SDS-樣品緩沖液中加熱至100℃，泳道4-6未加熱。泳道1和家兔抗肺炎衣原體OMC反應(yīng)；泳道2和4是前血清(pre-serum)；泳道3和5是多克隆家兔抗pEX1-1融合蛋白；泳道6是單克隆抗體26.1。
圖11顯示了肺炎衣原體的免疫印跡。泳道1-4，在SDS-緩沖液中加熱至100℃，泳道5-6未加熱。與107CFU的肺炎衣原體感染后14天的C57-黑色小鼠的血清反應(yīng)。泳道1和5，小鼠1；泳道2和6小鼠2；泳道3和5，小鼠3；泳道4和8，小鼠4。
圖12顯示了小鼠肺組織的免疫組織學(xué)分析，肺炎包涵體存在于支氣管上皮組織和肺胞壁中(箭頭所指處)。
實(shí)施例1編碼98/95 kDa肺炎衣原體COMC蛋白的基因的克隆肺炎衣原體EB和COMC的純化在HeLa細(xì)胞中培育肺炎衣原體。培育按照Miyashita和Matsumoto(1992)的說(shuō)明書(shū)進(jìn)行，只是略作修改，以100,000Xg離心上清液和后一次沉淀以及混濁的底層。通過(guò)1000xg離心30分鐘，使微生物附著于HeLa細(xì)胞，然后在5％CO2氣氛下在含有5％胎牛血清(FCS，Gibco BRL，Germany目錄號(hào)10106.169)慶大霉素的RPMI1640培養(yǎng)基(Gibco BRL，Germany目錄號(hào)51 800-27)中37℃培育2小時(shí)。將培養(yǎng)基換成另外還含有1毫克/毫升放線菌酮的培養(yǎng)基。培育48小時(shí)后，從培養(yǎng)中取出蓋玻片，用對(duì)肺炎衣原體有特異性的抗體(單克隆抗體26.1)(Christiansen等人，1994)和對(duì)種沙眼衣原體有特異性的單克隆抗體(單克隆抗體32.3，Loke diagnostics，ArhusDenmark)測(cè)試包涵體，以確保沒(méi)有發(fā)生沙眼衣原體污染。通過(guò)Hoechst染色并在BEa和BEg培養(yǎng)基(Freund等人，1979)中培養(yǎng)來(lái)測(cè)試HeLa細(xì)胞中有無(wú)支原體污染。另外，通過(guò)在BEa和BEg培養(yǎng)基中培育來(lái)測(cè)試肺炎衣原體原種中有無(wú)支原體污染。在培養(yǎng)物或細(xì)胞中沒(méi)有檢測(cè)到沙眼衣原體、支原體或細(xì)菌污染。感染后72小時(shí)，以PBS洗滌單層，在PBS中用橡膠刮棒打松細(xì)胞，用超聲法從宿主細(xì)胞中釋放出衣原體。在不連續(xù)密度梯度上純化肺炎衣原體EB和RB(Miyashita等人，1992)。用負(fù)染色和電子顯微鏡確認(rèn)衣原體EB的純度(圖1)，只檢測(cè)到符合肺炎衣原體EB結(jié)構(gòu)的大小為0.3-0.5mm的顆粒。如Caldwell等人(1981)中所述的那樣，對(duì)純化的衣原體EB進(jìn)行十二烷基肌氨酸鈉提取，改動(dòng)之處在于用簡(jiǎn)單的超聲破碎來(lái)懸浮COMC。用電子鏡和負(fù)染色測(cè)試純化的COMC(圖1)，從中看到折疊的外膜復(fù)合物。
SDS-PAGE分析純化的EB和COMC在15％SDS-聚丙烯酰胺凝膠上分離純化的EB和肺炎衣原體OMC中的蛋白，對(duì)凝膠銀染(圖2)，在泳道1中看到純化的EB含有100/95 kDa的主要蛋白和38kDa的蛋白，在純化的COMC(泳道2)中這兩種蛋白組也是主要的。另外，分子量為62/60kDa、55kDa和12kDa的蛋白已經(jīng)在COMC制備物中增濃。在比較純化的肺炎衣原體EB和純化的沙眼衣原體EB(泳道3)時(shí)，看到沙眼衣原體EB中主要的蛋白是主要外膜蛋白(MOMP)，它也是沙眼衣原體COMC制備物(泳道4)中的主要條帶，在該制備物中看到60/62kDa的Omp2和12kDa的Omp3。然而沒(méi)有檢測(cè)到如同肺炎衣原體COMC制備物的大小為100/95kDa的主要條帶。
抗肺炎衣原體COMC的家兔多克隆抗體的產(chǎn)生為了確保產(chǎn)生在免疫印跡和菌落印跡中識(shí)別所有肺炎衣原體蛋白的家兔抗體，將10微克COMC抗原溶解在20微升SDS樣品緩沖液中，然后分裝在5個(gè)小管中。用1毫升PBS和1毫升Freund不完全佐劑(Difco laboratories，USA目錄號(hào)0639-606)進(jìn)一步稀釋溶解的抗原，并注射新西蘭白兔四頭肌內(nèi)。以間隔一周時(shí)間肌內(nèi)注射三次，再過(guò)三周，靜脈內(nèi)注射稀釋在1毫升PBS中的溶解的COMC蛋白，兩周后重復(fù)該步驟。在開(kāi)始免疫后的11周后，從家兔獲得血清。用SDS-PAGE分離純化的肺炎衣原體EB，將蛋白電轉(zhuǎn)移到硝酸纖維素膜上。用多克隆COMC抗體封閉膜并免疫染色(圖3)。該血清識(shí)別EB制備物中大小為100/95，60和38kDa的蛋白。這與外膜蛋白的大小相符。
COMC蛋白的克隆由于在HeLa細(xì)胞中培育肺炎衣原體，因此EB制備物中可能存在污染性的宿主細(xì)胞DNA。因此，用DNA酶處理純化的EB制備物以除去污染性DNA。然后用CsCl梯度離心純化肺炎衣原體DNA。用Sau3A部分消化肺炎衣原體DNA，將含有大小約為0.5-0.4kb的DNA片段的級(jí)分克隆到表達(dá)載體系統(tǒng)pEX(Boehringer，Germany目錄號(hào)1034766，1034774，1034782)中。pEX載體系統(tǒng)具有β半乳糖苷酶基因，β-半乳糖苷酶基因的3′端有多個(gè)克隆位點(diǎn)。基因的表達(dá)由PR啟動(dòng)子調(diào)節(jié)，因此可以通過(guò)將溫度從32℃升高到42℃來(lái)誘導(dǎo)蛋白的表達(dá)。將重組細(xì)菌菌落轉(zhuǎn)移到硝酸纖維素膜上，將溫度升高至42℃2小時(shí)。將硝酸纖維素膜置于濾膜上浸泡在5％SDS中，使細(xì)菌裂解。用針對(duì)肺炎衣原體COMC的多克隆抗體檢測(cè)表達(dá)外膜蛋白的菌落。將陽(yáng)性克隆培育在懸浮液中并于42℃誘導(dǎo)2小時(shí)。用SDS-PAGE分析克隆的蛋白全貌，觀察到誘導(dǎo)的β半乳糖苷酶的大小增加(圖4)。另外，將蛋白電泳轉(zhuǎn)移到硝酸纖維素膜上，確認(rèn)與針對(duì)COMC的多克隆血清的反應(yīng)(圖5)。
陽(yáng)性COMC克隆的測(cè)序?yàn)榱藢?duì)pEX克隆進(jìn)行特性分析，對(duì)插入的肺炎衣原體DNA測(cè)序。在GenEmbl數(shù)據(jù)庫(kù)中針對(duì)原核序列搜索所得DNA序列。搜索結(jié)果鑒定了6個(gè)克隆是Omp2基因的部分，2個(gè)克隆是Omp3基因的部分，2個(gè)克隆是MOMP基因的部分，提示COMC蛋白已經(jīng)被成功克隆。另外，獲得32個(gè)克隆，其中含有在GenEmbl數(shù)據(jù)庫(kù)中未發(fā)現(xiàn)的DNA序列。然而，這些序列可能以兩個(gè)4-6個(gè)克隆的毗連群(contic)簇集，三個(gè)克隆是相同的。發(fā)現(xiàn)另外19個(gè)克隆與該毗連群不重疊(圖7)。為了獲得關(guān)于基因的更多的序列信息，用BamHI限制性酶整個(gè)消化肺炎衣原體DNA，將片段克隆到載體pBluescript中。將連接的DNA電轉(zhuǎn)化到大腸桿菌XL1-Blue中，并在含有氨芐青霉素的平板上選擇。將重組細(xì)菌菌落轉(zhuǎn)移到硝酸纖維素膜上，用pEX1-1克隆的插入物作為探針進(jìn)行菌落雜交。發(fā)現(xiàn)了含有4.5kb的單個(gè)BamHI片段的一個(gè)克隆，用Southern印跡確認(rèn)此克隆和探針雜交。用各約為300bp的合成引物對(duì)此克隆的插入物進(jìn)行雙向測(cè)序。BamHI片段的序列使得其能連接pEX克隆的兩個(gè)毗連群。和pEX克隆一起總共能裝配出編碼兩個(gè)新COMC蛋白的6.5kb DNA序列(圖6)。
采用來(lái)自已知Omp基因和來(lái)自其它已知基因的引物在純化的肺炎衣原體DNA上進(jìn)行PCR，獲得另外的序列。對(duì)獲得的PCR產(chǎn)物進(jìn)行測(cè)序。序列結(jié)構(gòu)顯示在圖7中。檢測(cè)到另外8個(gè)Omp基因。推導(dǎo)的氨基酸序列的序列對(duì)比顯示在圖8A和B中。
DNA序列的分析DNA序列編碼大小為89.6-100.3kDa的Omp4-15蛋白(Omp13為56.1kDa)。Omp4和Omp5以相反方向轉(zhuǎn)錄?？赡艿慕K止結(jié)構(gòu)位于Omp4下游。Omp5基因的3′端沒(méi)有被克隆，因?yàn)樵摶騼?nèi)存在BamHI限制性酶切位點(diǎn)。用GCG軟件包(Wisconsin軟件包，8.1-UNIX版，1995年8月，序列分析軟件包)的gap程序比較Omp4和Omp5的翻譯的DNA序列。兩個(gè)基因的氨基酸相同性為41％(相似性為61％)，在Omp4中，信號(hào)肽酶1的可能的斷裂位點(diǎn)在氨基酸17位，而在Omp5中在氨基酸25位。當(dāng)將其它兩個(gè)pEX克隆編碼的氨基酸序列與Omp4和Omp5相比時(shí)，它們與這些基因也具有氨基酸同源性。發(fā)現(xiàn)兩個(gè)克隆與Omp4和Omp5蛋白中的同一區(qū)域有同源性。因此，pEX克隆必定源自另兩個(gè)基因。因此將這些基因命名為Omp6和Omp7。對(duì)其它基因進(jìn)行類(lèi)似的分析。與Omp4和Omp5中所見(jiàn)相反，其它推定的Omp蛋白均沒(méi)有信號(hào)肽斷裂位點(diǎn)。
實(shí)施例2針對(duì)pEX融合蛋白以及加上Omp4的全長(zhǎng)重組的多克隆單特異性抗體為了調(diào)查Omp4-7蛋白的拓?fù)浣Y(jié)構(gòu)，從每個(gè)基因中選出代表性的pEX克隆。誘導(dǎo)出β-半乳糖苷酶/Omp的融合蛋白，如同包涵體那樣部分純化。用該抗原以一周的間隔對(duì)Balb/c小時(shí)肌內(nèi)免疫三次，6周后獲得小鼠血清。用肺炎衣原體感染HeLa細(xì)胞。感染72小時(shí)后用3.7％甲醛固定單層。由于外膜蛋白被甲醛廣泛交聯(lián)，因此該處理使得抗體不能透過(guò)衣原體外膜。用0.2％ Triton X100滲透HeLa細(xì)胞，用PBS洗滌單層，然后用20％(v/v)FCS培育，以滅活甲醛游離基團(tuán)。用含20％(v/v)FCS的PBS稀釋小鼠血清1∶100，并和單層一起培育半小時(shí)。用PBS洗滌單層，加入偶聯(lián)FITCH的第二家兔抗小鼠血清反應(yīng)半小時(shí)，洗滌單層并固定。一些抗體與包涵體中的EB強(qiáng)烈反應(yīng)(圖9)。盡管作了甲醛固定，但是也不能排除EB表面因處理而改變，這樣抗體能到達(dá)Omp4-7。因此，用針對(duì)克隆pEX3-36的抗體進(jìn)行免疫電子顯微鏡法確認(rèn)反應(yīng)。將純化的肺炎衣原體EB吸收到碳涂覆的鎳柵格上。吸收后，用PBS洗滌柵格，用溶解在PBS中的0.5％卵白蛋白封閉。用相同緩沖液1∶100稀釋抗體，并培育30分鐘。用PBS洗滌柵格。在柵格中加入用含1％明膠的PBS稀釋的偶聯(lián)10nm膠體金的家兔抗小鼠Ig，放置半小時(shí)。柵格用含1％明膠的PBS洗3次，再用PBS洗3次，柵格用0.7％磷鎢酸反染色(contrastain)。在40kV的Jeol 1010電鏡中分析柵格?？吹浇痤w粒覆蓋了純化的EB的表面。由于肺炎衣原體EB在純化或和抗體反應(yīng)條件下沒(méi)有接觸洗滌劑或固定，因此這些結(jié)果表明克隆的蛋白具有外露的表位。
針對(duì)Omp4的多克隆單特異性抗體用含有LIC位點(diǎn)的引物作PCR擴(kuò)增Omp4基因，將PCR產(chǎn)物克隆到pET-30 LIC載體(Novagen)中。通過(guò)IPTG誘導(dǎo)合成來(lái)表達(dá)組氨酸標(biāo)記的融合蛋白，并在鎳柱上純化。用純化的Omp4蛋白免疫家兔(6次，每次8微克)。
用針對(duì)重組體Omp4的家兔多克隆抗體檢測(cè)石蠟包埋切片中的肺炎衣原體在經(jīng)鼻感染后3天，獲得肺炎衣原體感染的小鼠的肺。將組織樣品固定在4％甲醛中，用石蠟包埋，切片并在染色前去除石蠟。使切片和以TBS 1∶200稀釋的家兔血清室溫培育30分鐘。在TBS中洗滌兩次后，用1∶300稀釋于TBS中的第二抗體(生物素化山羊抗家兔抗體)培育切片，然后用TBS洗滌兩次。用鏈霉親和素-生物素復(fù)合物(streptABCompelx/AP，Dako)對(duì)切片染色30分鐘，洗滌，并在鏡檢下用chromagen+new fuchsin(Vector Laboratories)顯影。用蘇木素對(duì)切片反染色，用顯微鏡分析。
用超免疫單特異性家兔抗血清進(jìn)行免疫印跡分析用含有LTC位點(diǎn)的引物PCR擴(kuò)增pEX1-1克隆的插入物。因此，PCR產(chǎn)物能插入pET-32 LIC載體(Novagen，UK目錄號(hào)69076-1)中。因此，pEX1-1克隆的插入物序列在新載體中以融合蛋白形式表達(dá)，由pET-32 LIC載體編碼的融合蛋白部分具有一排6個(gè)組氨酸殘基。在該載體中誘導(dǎo)融合蛋白的表達(dá)，由于組氨酸殘基對(duì)二價(jià)陽(yáng)離子有高親合力，因此該融合蛋白可在變性條件下在Ni2+柱上純化。用純化的蛋白免疫新西蘭白色家兔。肌內(nèi)免疫6次、靜脈內(nèi)免疫2次后，獲得家兔血清。將純化的肺炎衣原體EB溶解在SDS樣品緩沖液中。一半樣品在樣品緩沖液中加熱至100℃，而另一半樣品則不加熱。用SDS-PAGE分離樣品，將蛋白轉(zhuǎn)移到硝酸纖維素膜上，使血清和條帶反應(yīng)。對(duì)于加熱至100℃的樣品，血清識(shí)別約98kDa的高分子量條帶。這與Omp5的預(yù)計(jì)大小相符，pEX1-1克隆是其一部分，然而，當(dāng)抗體與未加熱EB條帶反應(yīng)時(shí)，圖形是不同的。現(xiàn)在發(fā)現(xiàn)了大小為75kDa的條帶，另外在條帶上方還觀察到較弱的條帶(圖1a)。這些數(shù)據(jù)證明，Omp5需要在SDS-樣品緩沖液中煮沸來(lái)完全變性，并遷移至從基因產(chǎn)物預(yù)計(jì)的大小處。當(dāng)樣品未煮沸時(shí)，蛋白沒(méi)有完全變性，更少的SDS與蛋白結(jié)合，它具有更具球形的結(jié)構(gòu)，其在丙烯酰胺凝膠中會(huì)遷移至更遠(yuǎn)處。條帶圖形看上去與用單克隆抗體(MAb26.1)的結(jié)果相同(泳道6)(我們以前已經(jīng)描述過(guò)(Christiansen等人，1994))，即與肺炎衣原體EB表面反應(yīng)，但是抗體不與免疫印跡中SDS完全變性的肺炎衣原體EB反應(yīng)。
C57黑色小鼠的實(shí)驗(yàn)性感染由于了解到?jīng)]有煮沸的Omp4-7蛋白的遷移發(fā)生改變，我們選擇在小鼠受實(shí)驗(yàn)性感染后分析針對(duì)肺炎衣原體EB的抗體。為了從肺炎衣原體引起的感染獲得抗體，在微量乙醚麻醉下用107CFI肺炎衣原體鼻內(nèi)接種C57黑色小鼠。感染14天后獲得血清樣品，分析肺的病理學(xué)變化。在兩個(gè)小鼠的肺切片中觀察到嚴(yán)重的肺炎，而在第3只小鼠中只發(fā)現(xiàn)有少量改變。1∶100稀釋小鼠血清，并和溶解在煮沸和沒(méi)有煮沸的樣品緩沖液中的純化的EB反應(yīng)。在已加熱至100℃的制備物中，來(lái)自兩只小鼠的血清與60/62kDa條帶以及55kDa的較弱條帶反應(yīng)強(qiáng)烈，但是對(duì)于大小為Omp4-7的蛋白沒(méi)有觀察到反應(yīng)(圖11)。然而，當(dāng)血清和未加熱過(guò)的制備物反應(yīng)時(shí)，它們和大小約為75kDa的寬條帶均有強(qiáng)反應(yīng)。這與未加熱制備物中Omp4-7蛋白的大小相符。因此，可以歸結(jié)為，肺炎衣原體感染后抗體識(shí)別的Omp4-7蛋白的表位是不連續(xù)的表位，因?yàn)榭乖耆冃酝耆茐牧吮砦?。在未加熱樣品中觀察到的75kD蛋白不是Omp2(如Omp2特異性抗體免疫印跡所表明)。
實(shí)施例3肺炎衣原體Omp4-7和鸚鵡熱衣原體推定的外膜蛋白(POMP)的比較Longbottom等人1996年已經(jīng)公開(kāi)了鸚鵡熱衣原體的98-90kDa的部分序列。他們已經(jīng)將該家族中5個(gè)基因的全序列輸入了EMBL數(shù)據(jù)庫(kù)。他們已將基因命名為“推定的外膜蛋白”(POMP)，因?yàn)檫€未確定它們的精確位置。該家族由兩個(gè)完全相同的基因組成，這兩個(gè)基因與這些基因高度同源。他們計(jì)算出分子大小為90和91kDa。第5個(gè)基因編碼98kDa的蛋白。用GCG軟件包的pileup程序比較肺炎衣原體Omp4-7蛋白的序列和鸚鵡熱衣原體POMP蛋白的序列。氨基酸同源性在51-63％范圍內(nèi)。發(fā)現(xiàn)肺炎衣原體Omp4-5蛋白與鸚鵡熱衣原體的98kDaPOMP蛋白最相關(guān)。令人感興趣的是，與其它鸚鵡熱衣原體基因相比，98kDa的鸚鵡熱衣原體POMP蛋白和肺炎衣原體基因更相關(guān)。GGAI的重復(fù)序列在98kDa POMP蛋白中是保守的，但是在90和91kDa鸚鵡熱衣原體POMP蛋白中只有3個(gè)GGAI重復(fù)序列。對(duì)于鸚鵡熱衣原體，已經(jīng)顯示，針對(duì)這些蛋白的抗體似乎對(duì)感染有保護(hù)作用。
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序列表(1)一般信息(i)申請(qǐng)人(A)姓名Svend Birkelund(B)街道Dept.of Medical Microbiology and Immunology，University of Arhus(C)城市Arhus C(D)省份(E)國(guó)家Denmark(F)郵政編碼(郵編)8000(ii)發(fā)明名稱肺炎衣原體抗原(iii)序列數(shù)目30(iv)計(jì)算機(jī)可讀形式(A)記錄介質(zhì)類(lèi)型軟盤(pán)(B)計(jì)算機(jī)IBM兼容型(C)操作系統(tǒng)DOS(D)軟件FastSEQ for Windows Version 2.0(v)本申請(qǐng)資料(A)申請(qǐng)?zhí)?2)SEQ ID NO1的信息(i)序列特征(A)長(zhǎng)度3200堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ix)特征(A)名稱/關(guān)鍵字編碼序列(B)位置205...2987(D)其它信息(xi)序列描述SEQ ID NO1CAATGTCGAA GAGAGCACTA ACCAGGAAAA TTGCGATTTC ATAAACCCAC TTTATTATTA 60AATTCTTACT TGCGTCATAT AAAATAGAAA ACTCAGAGAG TCAAGATAAA AATTCTTGAC 120AGCTGTTTTG TCATCTTTAA CTTGATTTAC TTATTTTGTT TCTATATTGA TGCGAATAGT 180TCTCTAAAAAA ACAAAAAGCAT TACC ATG AAG ACT TCG ATT CCT TGG GTT TTA 231Met Lys Thr Ser Ile Pro Trp Val Leu1 5GTT TCC TCC GTG TTA GCT TTC TCA TGT CAC CTA CAG TCA CTA GCT AAC 279Val Ser Ser Val Leu Ala Phe Ser Cys His Leu Gln Ser Leu Ala Asn10 15 20 25GAG GAA CTT TTA TCA CCT GAT GAT AGC TTT AAT GGA AAT ATC GAT TCA 327Glu Glu Leu Leu Ser Pro Asp Asp Ser Phe Asn Gly Asn Ile Asp Ser30 35 40GGA ACG TTT ACT CCA AAA ACT TCA GCC ACA ACA TAT TCT CTA ACA GGA 375Gly Thr Phe Thr Pro Lys Thr Ser Ala Thr Thr Tyr Ser Leu Thr Gly45 50 55GAT GTC TTC TTT TAC GAG CCT GGA AAA GGC ACT CCC TTA TCT GAC AGT 423Asp Val Phe Phe Tyr Glu Pro Gly Lys Gly Thr Pro Leu Ser Asp Ser60 65 70TGT TTT AAG CAA ACC ACG GAC AAT CTT ACC TTC TTG GGG AAC GGT CAT 471Cys Phe Lys Gln Thr Thr Asp Asn Leu Thr Phe Leu Gly Asn Gly His75 80 85AGC TTA ACG TTT GGC TTT ATA GAT GCT GGC ACT CAT GCA GGT GCT GCT 519Ser Leu Thr Phe Gly Phe Ile Asp Ala Gly Thr His Ala Gly Ala Ala90 95 100 105GCA TCT ACA ACA GCA AAT AAG AAT CTT ACC TTC TCA GGG TTT TCC TTA 567Ala Ser Thr Thr Ala Asn Lys Asn Leu Thr Phe Ser Gly Phe Ser Leu110 115 120CTG AGT TTT GAT TCC TCT CCT AGC ACA ACG GTT ACT ACA GGT CAG GGA 615Leu Ser Phe Asp Ser Ser Pro Ser Thr Thr Val Thr Thr Gly Gln Gly125 130 135ACG CTT TCC TCA GCA GGA GGC GTA AAT TTA GAA AAT ATT CGT AAA CTT 663Thr Leu Ser Ser Ala Gly Gly Val Asn Leu Glu Asn Ile Arg Lys Leu140 145 150GTA GTT GCT GGG AAT TTT TCT ACT GCA GAT GGT GGA GCT ATC AAA GGA 711Val Val Ala Gly Asn Phe Ser Thr Ala Asp Gly Gly Ala Ile Lys Gly155 160 165GCG TCT TTC CTT TTA ACT GGC ACT TCT GGA GAT GCT CTT TTT AGT AAC 759Ala Ser Phe Leu Leu Thr Gly Thr Ser Gly Asp Ala Leu Phe Ser Asn170 175 180 185AAC TCT TCA TCA ACA AAG GGA GGA GCA ATT GCT ACT ACA GCA GGC GCT 807Asn Ser Ser Ser Thr Lys Gly Gly Ala Ile Ala Thr Thr Ala Gly Ala190 195 200CGC ATA GCA AAT AAC ACA GGT TAT GTT AGA TTC CTA TCT AAC ATA GCG 855Arg Ile Ala Asn Asn Thr Gly Tyr Val Arg Phe Leu Ser Asn Ile Ala205 210 215TCT ACG TCA GGA GGC GCT ATC GAT GAT GAA GGC ACG TCG ATA CTA TCG 903Ser Thr Ser Gly Gly Ala Ile Asp Asp Glu Gly Thr Ser Ile Leu Ser
220 225 230AAC AAC AAA TTT CTA TAT TTT GAA GGG AAT GCA GCG AAA ACT ACT GGC 951Asn Asn Lys Phe Leu Tyr Phe Glu Gly Asn Ala Ala Lys Thr Thr Gly235 240 245GGT GCG ATC TGC AAC ACC AAG GCG AGT GGA TCT CCT GAA CTG ATA ATC 999Gly Ala Ile Cys Asn Thr Lys Ala Ser Gly Ser Pro Glu Leu Ile Ile250 255 260 265TCT AAC AAT AAG ACT CTG ATC TTT GCT TCA AAC GTA GCA GAA ACA AGC 1047Ser Asn Asn Lys Thr Leu Ile Phe Ala Ser Asn Val Ala Glu Thr Ser270 275 280GGT GGC GCC ATC CAT GCT AAA AAG CTA GCC CTT TCC TCT GGA GGC TTT 1095Gly Gly Ala Ile His Ala Lys Lys Leu Ala Leu Ser Ser Gly Gly Phe285 290 295ACA GAG TTT CTA CGA AAT AAT GTC TCA TCA GCA ACT CCT AAG GGG GGT 1143Thr Glu Phe Leu Arg Asn Asn Val Ser Ser Ala Thr Pro Lys Gly Gly300 305 310GCT ATC AGC ATC GAT GCC TCA GGA GAG CTC AGT CTT TCT GCA GAG ACA 1191Ala Ile Ser Ile Asp Ala Ser Gly Glu Leu Ser Leu Ser Ala Glu Thr315 320 325GGA AAC ATT ACC TTT GTA AGA AAT ACC CTT ACA ACA ACC GGA AGT ACC 1239Gly Asn Ile Thr Phe Val Arg Asn Thr Leu Thr Thr Thr Gly Ser Thr330 335 340 345GAT ACT CCT AAA CGT AAT GCG ATC AAC ATA GGA AGT AAC GGG AAA TTC 1287Asp Thr Pro Lys Arg Asn Ala Ile Asn Ile Gly Ser Asn Gly Lys Phe350 355 360ACG GAA TTA CGG GCT GCT AAA AAT CAT ACA ATT TTC TTC TAT GAT CCC 1335Thr Glu Leu Arg Ala Ala Lys Asn His Thr Ile Phe Phe Tyr Asp Pro365 370 375ATC ACT TCA GAA GGA ACC TCA TCA GAC GTA TTG AAG ATA AAT AAC GGC 1383Ile Thr Ser Glu Gly Thr Ser Ser Asp Val Leu Lys Ile Asn Asn Gly380 385 390TCT GCG GGA GCT CTC AAT CCA TAT CAA GGA ACG ATT CTA TTT TCT GGA 1431Ser Ala Gly Ala Leu Asn Pro Tyr Gln Gly Thr Ile Leu Phe Ser Gly395 400 405GAA ACC CTA ACA GCA GAT GAA CTT AAA GTT GCT GAC AAT TTA AAA TCT 1479Glu Thr Leu Thr Ala Asp Glu Leu Lys Val Ala Asp Asn Leu Lys Ser410 415 420 425TCA TTC ACG CAG CCA GTC TCC CTA TCC GGA GGA AAG TTA TTG CTA CAA 1527Ser Phe Thr Gln Pro Val Ser Leu Ser Gly Gly Lys Leu Leu Leu Gln
430 435 440AAG GGA GTC ACT TTA GAG AGC ACG AGC TTC TCT CAA GAG GCC GGT TCT 1575Lys Gly Val Thr Leu Glu Ser Thr Ser Phe Ser Gln Glu Ala Gly Ser445 450 455CTC CTC GGC ATG GAT TCA GGA ACG ACA TTA TCA ACT ACA GCT GGG AGT 1623Leu Leu Gly Met Asp Ser Gly Thr Thr Leu Ser Thr Thr Ala Gly Ser460 465 470ATT ACA ATC ACG AAC CTA GGA ATC AAT GTT GAC TCC TTA GGT CTT AAG 1671Ile Thr Ile Thr Asn Leu Gly Ile Asn Val Asp Ser Leu Gly Leu Lys475 480 485CAG CCC GTC AGC CTA ACA GCA AAA GGT GCT TCA AAT AAA GTG ATC GTA 1719Gln Pro Val Ser Leu Thr Ala Lys Gly Ala Ser Asn Lys Val Ile Val490 495 500 505TCT GGG AAG CTC AAC CTG ATT GAT ATT GAA GGG AAC ATT TAT GAA AGT 1767Ser Gly Lys Leu Asn Leu Ile Asp Ile Glu Gly Asn Ile Tyr Glu Ser510 515 520CAT ATG TTC AGC CAT GAC CAG CTC TTC TCT CTA TTA AAA ATC ACG GTT 1815His Met Phe Ser His Asp Gln Leu Phe Ser Leu Leu Lys Ile Thr Val525 530 535GAT GCT GAT GTT GAT ACT AAC GTT GAC ATC AGC AGC CTT ATC CCT GTT 1863Asp Ala Asp Val Asp Thr Asn Val Asp Ile Ser Ser Leu Ile Pro Val540 545 550CCT GCT GAG GAT CCT AAT TCA GAA TAC GGA TTC CAA GGA CAA TGG AAT 1911Pro Ala Glu Asp Pro Asn Ser Glu Tyr Gly Phe Gln Gly Gln Trp Asn555 560 565GTT AAT TGG ACT ACG GAT ACA GCT ACA AAT ACA AAA GAG GCC ACG GCA 1959Val Asn Trp Thr Thr Asp Thr Ala Thr Asn Thr Lys Glu Ala Thr Ala570 575 580 585ACT TGG ACC AAA ACA GGA TTT GTT CCC AGC CCC GAA AGA AAA TCT GCG 2007Thr Trp Thr Lys Thr Gly Phe Val Pro Ser Pro Glu Arg Lys Ser Ala590 595 600TTA GTA TGC AAT ACC CTA TGG GGA GTC TTT ACT GAC ATT CGC TCT CTG 2055Leu Val Cys Asn Thr Leu Trp Gly Val Phe Thr Asp Ile Arg Ser Leu605 610 615CAA CAG CTT GTA GAG ATC GGC GCA ACT GGT ATG GAA CAC AAA CAA GGT 2103Gln Gln Leu Val Glu Ile Gly Ala Thr Gly Met Glu His Lys Gln Gly620 625 630TTC TGG GTT TCC TCC ATG ACG AAC TTC CTG CAT AAG ACT GGA GAT GAA 2151Phe Trp Val Ser Ser Met Thr Asn Phe Leu His Lys Thr Gly Asp Glu
635 640 645AAT CGC AAA GGC TTC CGT CAT ACC TCT GGA GGC TAC GTC ATC GGT GGA 2199Asn Arg Lys Gly Phe Arg His Thr Ser Gly Gly Tyr Val Ile Gly Gly650 655 660 665AGT GCT CAC ACT CCT AAA GAC GAC CTA TTT ACC TTT GCG TTC TGC CAT 2247Ser Ala His Thr Pro Lys Asp Asp Leu Phe Thr Phe Ala Phe Cys His670 675 680CTC TTT GCT AGA GAC AAA GAT TGT TTT ATC GCT CAC AAC AAC TCT AGA 2295Leu Phe Ala Arg Asp Lys Asp Cys Phe Ile Ala His Asn Asn Ser Arg685 690 695ACC TAC GGT GGA ACT TTA TTC TTC AAG CAC TCT CAT ACC CTA CAA CCC 2343Thr Tyr Gly Gly Thr Leu Phe Phe Lys His Ser His Thr Leu Gln Pro700 705 710CAA AAC TAT TTG AGA TTA GGA AGA GCA AAG TTT TCT GAA TCA GCT ATA 2391Gln Asn Tyr Leu Arg Leu Gly Arg Ala Lys Phe Ser Glu Ser Ala Ile715 720 725GAA AAA TTC CCT AGG GAA ATT CCC CTA GCC TTG GAT GTC CAA GTT TCG 2439Glu Lys Phe Pro Arg Glu Ile Pro Leu Ala Leu Asp Val Gln Val Ser730 735 740 745TTC AGC CAT TCA GAC AAC CGT ATG GAA ACG CAC TAT ACC TCA TTG CCA 2487Phe Ser His Ser Asp Asn Arg Met Glu Thr His Tyr Thr Ser Leu Pro750 755 760GAA TCC GAA GGT TCT TGG AGC AAC GAG TGT ATA GCT GGT GGT ATC GGC 2535Glu Ser Glu Gly Ser Trp Ser Asn Glu Cys Ile Ala Gly Gly Ile Gly765 770 775CTA GAC CTT CCT TTT GTT CTT TCC AAC CCA CAT CCT CTT TTC AAG ACC 2583Leu Asp Leu Pro Phe Val Leu Ser Asn Pro His Pro Leu Phe Lys Thr780 785 790TTC ATT CCA CAG ATG AAA GTC GAA ATG GTT TAT GTA TCA CAA AAT AGC 2631Phe Ile Pro Gln Met Lys Val Glu Met Val Tyr Val Ser Gln Asn Ser795 800 805TTC TTC GAA AGC TCT AGT GAT GGC CGT GGT TTT AGT ATT GGA AGG CTG 2679Phe Phe Glu Ser Ser Ser Asp Gly Arg Gly Phe Ser Ile Gly Arg Leu810 815 820 825CTT AAC CTC TCG ATT CCT GTG GGT GCG AAA TTC GTG CAG GGG GAT ATC 2727Leu Asn Leu Ser Ile Pro Val Gly Ala Lys Phe Val Gln Gly Asp Ile830 835 840GGA GAT TCC TAC ACC TAT GAT CTC TCA GGA TTC TTT GTT TCC GAT GTC 2775Gly Asp Ser Tyr Thr Tyr Asp Leu Ser Gly Phe Phe Val Ser Asp Val
845 850 855TAT CGT AAC AAT CCC CAA TCT ACA GCG ACT CTT GTG ATG AGC CCA GAC 2823Tyr Arg Asn Asn Pro Gln Ser Thr Ala Thr Leu Val Met Ser Pro Asp860 865 870TCT TGG AAA ATT CGC GGT GGC AAT CTT TCA AGA CAG GCA TTT TTA CTG 2871Ser Trp Lys Ile Arg Gly Gly Asn Leu Ser Arg Gln Ala Phe Leu Leu875 880 885AGG GGT AGC AAC AAC TAC GTC TAC AAC TCC AAT TGT GAG CTC TTC GGA 2919Arg Gly Ser Asn Asn Tyr Val Tyr Asn Ser Asn Cys Glu Leu Phe Gly890 895 900 905CAT TAC GCT ATG GAA CTC CGT GGA TCT TCA AGG AAC TAC AAT GTA GAT 2967His Tyr Ala Met Glu Leu Arg Gly Ser Ser Arg Asn Tyr Asn Val Asp910 915 920GTT GGT ACC AAA CTC CGA TT CTAGATTGCT AAAACTCCCT AGTTCTTCTA GGGAG 3022Val Gly Thr Lys Leu Arg Phe925TTTTCTCATA CTTTTAGGGA AATATTTGCT ATAGGGAATG CTTTCCTTGC AAACTGTAAA 3082AAATAACATT TGTCCCTCTT CAAAAAAGAT TTCTTTTAAT AATTTCTAGT TATAATTTTA 3142TTTTAAAAAC AGTTAAATAA TTAATAGACA ATAATCTATT CTTATTGACT TCTTTTTT 3200(2)SEQ ID NO2的信息(i)序列特征(A)長(zhǎng)度928氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型蛋白質(zhì)(v)片段類(lèi)型內(nèi)部(xi)序列描述SEQ ID NO2Met Lys Thr Ser Ile Pro Trp Val Leu Val Ser Ser Val Leu Ala Phe1 5 10 15Ser Cys His Leu Gln Ser Leu Ala Asn Glu Glu Leu Leu Ser Pro Asp20 25 30Asp Ser Phe Asn Gly Asn Ile Asp Ser Gly Thr Phe Thr Pro Lys Thr35 40 45Ser Ala Thr Thr Tyr Ser Leu Thr Gly Asp Val Phe Phe Tyr Glu Pro50 55 60Gly Lys Gly Thr Pro Leu Ser Asp Ser Cys Phe Lys Gln Thr Thr Asp65 70 75 80Asn Leu Thr Phe Leu Gly Asn Gly His Ser Leu Thr Phe Gly Phe Ile85 90 95Asp Ala Gly Thr His Ala Gly Ala Ala Ala Ser Thr Thr Ala Asn Lys
100 105 110Asn Leu Thr Phe Ser Gly Phe Ser Leu Leu Ser Phe Asp Ser Ser Pro115 120 125Ser Thr Thr Val Thr Thr Gly Gln Gly Thr Leu Ser Ser Ala Gly Gly130 135 140Val Asn Leu Glu Asn Ile Arg Lys Leu Val Val Ala Gly Asn Phe Ser145 150 155 160Thr Ala Asp Gly Gly Ala Ile Lys Gly Ala Ser Phe Leu Leu Thr Gly165 170 175Thr Ser Gly Asp Ala Leu Phe Ser Asn Asn Ser Ser Ser Thr Lys Gly180 185 190Gly Ala Ile Ala Thr Thr Ala Gly Ala Arg Ile Ala Asn Asn Thr Gly195 200 205Tyr Val Arg Phe Leu Ser Asn Ile Ala Ser Thr Ser Gly Gly Ala Ile210 215 220Asp Asp Glu Gly Thr Ser Ile Leu Ser Asn Asn Lys Phe Leu Tyr Phe225 230 235 240Glu Gly Asn Ala Ala Lys Thr Thr Gly Gly Ala Ile Cys Asn Thr Lys245 250 255Ala Ser Gly Ser Pro Glu Leu Ile Ile Ser Asn Asn Lys Thr Leu Ile260 265 270Phe Ala Ser Asn Val Ala Glu Thr Ser Gly Gly Ala Ile His Ala Lys275 280 285Lys Leu Ala Leu Ser Ser Gly Gly Phe Thr Glu Phe Leu Arg Asn Asn290 295 300Val Ser Ser Ala Thr Pro Lys Gly Gly Ala Ile Ser Ile Asp Ala Ser305 310 315 320Gly Glu Leu Ser Leu Ser Ala Glu Thr Gly Asn Ile Thr Phe Val Arg325 330 335Asn Thr Leu Thr Thr Thr Gly Ser Thr Asp Thr Pro Lys Arg Asn Ala340 345 350Ile Asn Ile Gly Ser Asn Gly Lys Phe Thr Glu Leu Arg Ala Ala Lys355 360 365Asn His Thr Ile Phe Phe Tyr Asp Pro Ile Thr Ser Glu Gly Thr Ser370 375 380Ser Asp Val Leu Lys Ile Asn Asn Gly Ser Ala Gly Ala Leu Asn Pro385 390 395 400Tyr Gln Gly Thr Ile Leu Phe Ser Gly Glu Thr Leu Thr Ala Asp Glu405 410 415Leu Lys Val Ala Asp Asn Leu Lys Ser Ser Phe Thr Gln Pro Val Ser420 425 430Leu Ser Gly Gly Lys Leu Leu Leu Gln Lys Gly Val Thr Leu Glu Ser435 440 445Thr Ser Phe Ser Gln Glu Ala Gly Ser Leu Leu Gly Met Asp Ser Gly450 455 460Thr Thr Leu Ser Thr Thr Ala Gly Ser Ile Thr Ile Thr Asn Leu Gly465 470 475 480Ile Asn Val Asp Ser Leu Gly Leu Lys Gln Pro Val Ser Leu Thr Ala485 490 495Lys Gly Ala Ser Asn Lys Val Ile Val Ser Gly Lys Leu Asn Leu Ile500 505 510Asp Ile Glu Gly Asn Ile Tyr Glu Ser His Met Phe Ser His Asp Gln
515 520 525Leu Phe Ser Leu Leu Lys Ile Thr Val Asp Ala Asp Val Asp Thr Asn530 535 540Val Asp Ile Ser Ser Leu Ile Pro Val Pro Ala Glu Asp Pro Asn Ser545 550 555 560Glu Tyr Gly Phe Gln Gly Gln Trp Asn Val Asn Trp Thr Thr Asp Thr565 570 575Ala Thr Asn Thr Lys Glu Ala Thr Ala Thr Trp Thr Lys Thr Gly Phe580 585 590Val Pro Ser Pro Glu Arg Lys Ser Ala Leu Val Cys Asn Thr Leu Trp595 600 605Gly Val Phe Thr Asp Ile Arg Ser Leu Gln Gln Leu Val Glu Ile Gly610 615 620Ala Thr Gly Met Glu His Lys Gln Gly Phe Trp Val Ser Ser Met Thr625 630 635 640Asn Phe Leu His Lys Thr Gly Asp Glu Asn Arg Lys Gly Phe Arg His645 650 655Thr Ser Gly Gly Tyr Val Ile Gly Gly Ser Ala His Thr Pro Lys Asp660 665 670Asp Leu Phe Thr Phe Ala Phe Cys His Leu Phe Ala Arg Asp Lys Asp675 680 685Cys Phe Ile Ala His Asn Asn Ser Arg Thr Tyr Gly Gly Thr Leu Phe690 695 700Phe Lys His Ser His Thr Leu Gln Pro Gln Asn Tyr Leu Arg Leu Gly705 710 715 720Arg Ala Lys Phe Ser Glu Ser Ala Ile Glu Lys Phe Pro Arg Glu Ile725 730 735Pro Leu Ala Leu Asp Val Gln Val Ser Phe Ser His Ser Asp Asn Arg740 745 750Met Glu Thr His Tyr Thr Ser Leu Pro Glu Ser Glu Gly Ser Trp Ser755 760 765Asn Glu Cys Ile Ala Gly Gly Ile Gly Leu Asp Leu Pro Phe Val Leu770 775 780Ser Asn Pro His Pro Leu Phe Lys Thr Phe Ile Pro Gln Met Lys Val785 790 795 800Glu Met Val Tyr Val Ser Gln Asn Ser Phe Phe Glu Ser Ser Ser Asp805 810 815Gly Arg Gly Phe Ser Ile Gly Arg Leu Leu Asn Leu Ser Ile Pro Val820 825 830Gly Ala Lys Phe Val Gln Gly Asp Ile Gly Asp Ser Tyr Thr Tyr Asp835 840 845Leu Ser Gly Phe Phe Val Ser Asp Val Tyr Arg Asn Asn Pro Gln Ser850 855 860Thr Ala Thr Leu Val Met Ser Pro Asp Ser Trp Lys Ile Arg Gly Gly865 870 875 880Asn Leu Ser Arg Gln Ala Phe Leu Leu Arg Gly Ser Asn Asn Tyr Val885 890 895Tyr Asn Ser Asn Cys Glu Leu Phe Gly His Tyr Ala Met Glu Leu Arg900 905 910Gly Ser Ser Arg Asn Tyr Asn Val Asp Val Gly Thr Lys Leu Arg Phe915 920 925
(2)SEQ ID NO3的信息(i)序列特征(A)長(zhǎng)度2815堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO3ATGAAATCGC AATTTTCCTG GTTAGTGCTC TCTTCGACAT TGGCATGTTT TACTAGTTGT 60TCCACTGTTT TTGCTGCAAC TGCTGAAAAT ATAGGCCCCT CTGATAGCTT TGACGGAAGT 120ACTAACACAG GCACCTATAC TCCTAAAAAT ACGACTACTG GAATAGACTA TACTCTGACA 180GGAGATATAA CTCTGCAAAA CCTTGGGGAT TCGGCAGCTT TAACGAAGGG TTGTTTTTCT 240GACACTACGG AATCTTTAAG CTTTGCCGGT AAGGGGTACT CACTTTCTTT TTTAAATATT 300AAGTCTAGTG CTGAAGGCGC AGCACTTTCT GTTACAACTG ATAAAAATCT GTCGCTAACA 360GGATTTTCGA GTCTTACTTT CTTAGCGGCC CCATCATCGG TAATCACAAC CCCCTCAGGA 420AAAGGTGCAG TTAAATGTGG AGGGGATCTT ACATTTGATA ACAATGGAAC TATTTTATTT 480AAACAAGATT ACTGTGAGGA AAATGGCGGA GCCATTTCTA CCAAGAATCT TTCTTTGAAA 540AACAGCACGG GATCGATTTC TTTTGAAGGG AATAAATCGA GCGCAACAGG GAAAAAAGGT 600GGGGCTATTT GTGCTACTGG TACTGTAGAT ATTACAAATA ATACGGCTCC TACCCTCTTC 660TCGAACAATA TTGCTGAAGC TGCAGGTGGA GCTATAAATA GCACAGGAAA CTGTACAATT 720ACAGGGAATA CGTCTCTTGT ATTTTCTGAA AATAGTGTGA CAGCGACCGC AGGAAATGGA 780GGAGCTCTTT CTGGAGATGC CGATGTTACC ATATCTGGGA ATCAGAGTGT AACTTTCTCA 840GGAAACCAAG CTGTAGCTAA TGGCGGAGCC ATTTATGCTA AGAAGCTTAC ACTGGCTTCC 900GGGGGGGGGG GGGGTATCTC CTTTTCTAAC AATATAGTCC AAGGTACCAC TGCAGGTAAT 960GGTGGAGCCA TTTCTATACT GGCAGCTGGA GAGTGTAGTC TTTCAGCAGA AGCAGGGGAC 1020ATTACCTTCA ATGGGAATGC CATTGTTGCA ACTACACCAC AAACTACAAA AAGAAATTCT 1080ATTGACATAG GATCTACTGC AAAGATCACG AATTTACGTG CAATATCTGG GCATAGCATC 1140TTTTTCTACG ATCCGATTAC TGCTAATACG GCTGCGGATT CTACAGATAC TTTAAATCTC 1200AATAAGGCTG ATGCAGGTAA TAGTACAGAT TATAGTGGGT CGATTGTTTT TTCTGGTGAA 1260AAGCTCTCTG AAGATGAAGC AAAAGTTGCA GACAACCTCA CTTCTACGCT GAAGCAGCCT 1320GTAACTCTAA CTGCAGGAAA TTTAGTACTT AAACGTGGTG TCACTCTCGA TACGAAAGGC 1380TTTACTCAGA CCGCGGGTTC CTCTGTTATT ATGGATGCGG GCACAACGTT AAAAGCAAGT 1440ACAGAGGAGG TCACTTTAAC AGGTCTTTCC ATTCCTGTAG ACTCTTTAGG CGAGGGTAAG 1500AAAGTTGTAA TTGCTGCTTC TGCAGCAAGT AAAAATGTAG CCCTTAGTGG TCCGATTCTT 1560CTTTTGGATA ACCAAGGGAA TGCTTATGAA AATCACGACT TAGGAAAAAC TCAAGACTTT 1620TCATTTGTGC AGCTCTCTGC TCTGGGTACT GCAACAACTA CAGATGTTCC AGCGGTTCCT 1680ACAGTAGCAA CTCCTACGCA CTATGGGTAT CAAGGTACTT GGGGAATGAC TTGGGTTGAT 1740GATACCGCAA GCACTCCAAA GACTAAGACA GCGACATTAG CTTGGACCAA TACAGGCTAC 1800CTTCCGAATC CTGAGCGTCA AGGACCTTTA GTTCCTAATA GCCTTTGGGG ATCTTTTTCA 1860GACATCCAAG CGATTCAAGG TGTCATAGAG AGAAGTGCTT TGACTCTTTG TTCAGATCGA 1920GGCTTCTGGG CTGCGGGAGT CGCCAATTTC TTAGATAAAG ATAAGAAAGG GGAAAAACGC 1980AAATACCGTC ATAAATCTGG TGGATATGCT ATCGGAGGTG CAGCGCAAAC TTGTTCTGAA 2040AACTTAATTA GCTTTGCCTT TTGCCAACTC TTTGGTAGCG ATAAAGATTT CTTAGTCGCT 2100AAAAATCATA CTGATACCTA TGCAGGAGCC TTCTATATCC AACACATTAC AGAATGTAGT 2160GGGTTCATAG GTTGTCTCTT AGATAAACTT CCTGGCTCTT GGAGTCATAA ACCCCTCGTT 2220TTAGAAGGGC AGCTCGCTTA TAGCCACGTC AGTAATGATC TGAAGACAAA GTATACTGCG 2280TATCCTGAGG TGAAAGGTTC TTGGGGGAAT AATGCTTTTA ACATGATGTT GGGAGCTTCT 2340TCTCATTCTT ATCCTGAATA CCTGCATTGT TTTGATACCT ATGCTCCATA CATCAAACTG 2400AATCTGACCT ATATACGTCA GGACAGCTTC TCGGAGAAAG GTACAGAAGG AAGATCTTTT 2460GATGACAGCA ACCTCTTCAA TTTATCTTTG CCTATAGGGG TGAAGTTTGA GAAGTTCTCT 2520GATTGTAATG ACTTTTCTTA TGATCTGACT TTATCCTATG TTCCTGATCT TATCCGCAAT 2580GATCCCAAAT GCACTACAGC ACTTGTAATC AGCGGAGCCT CTTGGGAAAC TTATGCCAAT 2640AACTTAGCAC GACAGGCCTT GCAAGTGCGT GCAGGCAGTC ACTACGCCTT CTCTCCTATG 2700TTTGAAGTGC TCGGCCAGTT TGTCTTTGAA GTTCGTGGAT CCTCACGGAT TTATAATGTA 2760GATCTTGGGG GTAAGTTCCA ATTCTAGGAG CGTCTCTCAT GTCTCAGAAA TTCTG 2815(2)SEQ ID NO4的信息(i)序列特征(A)長(zhǎng)度928氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO4Met Lys Ser Gln Phe Ser Trp Leu Val Leu Ser Ser Thr Leu Ala Cys1 5 10 15Phe Thr Ser Cys Ser Thr ValPhe Ala Ala Thr Ala Glu Asn Ile Gly20 25 30Pro Ser Asp Ser Phe Asp Gly Ser Thr Asn Thr Gly Thr Tyr Thr Pro35 40 45Lys Asn Thr Thr Thr Gly Ile Asp Tyr Thr Leu Thr Gly Asp Ile Thr50 55 60Leu Gln Asn Leu Gly Asp Ser Ala Ala Leu Thr Lys Gly Cys Phe Ser65 70 75 80Asp Thr Thr Glu Ser Leu Ser Phe Ala Gly Lys Gly Tyr Ser Leu Ser85 90 95Phe Leu Asn Ile Lys Ser Ser Ala Glu Gly Ala Ala Leu Ser Val Thr100 105 110Thr Asp Lys Asn Leu Ser Leu Thr Gly Phe Ser Ser Leu Thr Phe Leu115 120 125Ala Ala Pro Ser Ser Val Ile Thr Thr Pro Ser Gly Lys Gly Ala Val130 135 140Lys Cys Gly Gly Asp Leu Thr Phe Asp Asn Asn Gly Thr Ile Leu Phe145 150 155 160Lys Gln Asp Tyr Cys Glu Glu Asn Gly Gly Ala Ile Ser Thr Lys Asn165 170 175Leu Ser Leu Lys Asn Ser Thr Gly Ser Ile Ser Phe Glu Gly Asn Lys180 185190Ser Ser Ala Thr Gly Lys Lys Gly Gly Ala Ile Cys Ala Thr Gly Thr195 200 205Val Asp Ile Thr Asn Asn Thr Ala Pro Thr Leu Phe Ser Asn Asn Ile210 215 220Ala Glu Ala Ala Gly Gly Ala Ile Asn Ser Thr Gly Asn Cys Thr Ile225 230 235 240Thr Gly Asn Thr Ser Leu Val Phe Ser Glu Asn Ser Val Thr Ala Thr245 250 255Ala Gly Asn Gly Gly Ala Leu Ser Gly Asp Ala Asp Val Thr Ile Ser260 265 270Gly Asn Gln Ser Val Thr Phe Ser Gly Asn Gln Ala Val Ala Asn Gly275 280 285Gly Ala Ile Tyr Ala Lys Lys Leu Thr Leu Ala Ser Gly Gly Gly Gly290 295 300Gly Ile Ser Phe Ser Asn Asn He Val Gln Gly Thr Thr Ala Gly Asn305 310315 320Gly Gly Ala Ile Ser Ile Leu Ala Ala Gly Gh Cys Ser Leu Ser Ala325 330335Gh Ala Gly Asp Ile Thr Phe Asn Gly Asn Ala Ile Val Ala Thr Thr340 345 350Pro Gln Thr Thr Lys Arg Asn Ser Ile Asp Ile Gly Ser Thr Ala Lys355 360 365Ile Thr Asn Leu Arg Ala Ile Ser Gly His Ser Ile Phe Phe Tyr Asp370 375 380Pro Ile Thr Ala Asn Thr Ala Ala Asp Ser Thr Asp Thr Leu Asn Leu385 390 395 400Asn Lys Ala Asp Ala Gly Asn Ser Thr Asp Tyr Ser Gly Ser Ile Val405 410 415Phe Ser Gly Glu Lys Leu Ser Glu Asp Glu Ala Lys Val Ala Asp Asn420 425430Leu Thr Ser Thr Leu Lys Gln Pro ValThr Leu Thr Ala Gly Asn Leu435 440445Val Leu Lys Arg Gly Val Thr Leu Asp Thr Lys Gly Phe Thr Gln Thr450 455 460Ala Gly Ser Ser Val Ile Met Asp Ala Gly Thr Thr Leu Lys Ala Ser465 470 475 480Thr Glu Glu Val Thr Leu Thr Gly Leu Ser Ile Pro Val Asp Ser Leu485 490 495Gly Glu Gly Lys Lys Val Val Ile Ala Ala Ser Ala Ala Ser Lys Asn500 505 510Val Ala Leu Ser Gly Pro Ile Leu Leu Leu Asp Asn Gln Gly Asn Ala515 520 525Tyr Glu Asn His Asp Leu Gly Lys Thr Gln Asp Phe Ser Phe Val Gln530 535 540Leu Ser Ala Leu Gly Thr Ala Thr Thr Thr Asp Val Pro Ala Val Pro545 550 555 560Thr Val Ala Thr Pro Thr His Tyr Gly Tyr Gln Gly Thr Trp Gly Met565 570 575Thr Trp Val Asp Asp Thr Ala Ser Thr Pro Lys Thr Lys Thr Ala Thr580 585 590Leu Ala Trp Thr Asn Thr Gly Tyr Leu Pro Asn Pro Glu Arg Gln Gly595 600 605Pro Leu Val Pro Asn Ser Leu Trp Gly Ser Phe Ser Asp Ile Gln Ala610 615 620Ile Gln Gly Val Ile Glu Arg Ser Ala Leu Thr Leu Cys Ser Asp Arg625 630 635 640Gly Phe Trp Ala Ala Gly Val Ala Asn Phe Leu Asp Lys Asp Lys Lys
645 650 655Gly Glu Lys Arg Lys Tyr Arg His Lys Ser Gly Gly Tyr Ala Ile Gly660 665 670Gly Ala Ala Gln Thr Cys Ser Glu Asn Leu Ile Ser Phe Ala Phe Cys675 680 685Gln Leu Phe Gly Ser Asp Lys Asp Phe Leu Val Ala Lys Asn His Thr690 695 700Asp Thr Tyr Ala Gly Ala Phe Tyr Ile Gln His Ile Thr Glu Cys Ser705 710 715 720Gly Phe Ile Gly Cys Leu Leu Asp Lys Leu Pro Gly Ser Trp Ser His725 730 735Lys Pro Leu Val Leu Glu Gly Gln Leu Ala Tyr Ser His Val Ser Asn740 745 750Asp Leu Lys Thr Lys Tyr Thr Ala Tyr Pro Glu Val Lys Gly Ser Trp755 760 765Gly Asn Asn Ala Phe Asn Met Met Leu Gly Ala Ser Ser His Ser Tyr770 775 780Pro Glu Tyr Leu His Cys Phe Asp Thr Tyr Ala Pro Tyr Ile Lys Leu785 790 795 800Asn Leu Thr Tyr Ile Arg Gln Asp Ser Phe Ser Glu Lys Gly Thr Glu805 810 815Gly Arg Ser Phe Asp Asp Ser Asn Leu Phe Asn Leu Ser Leu Pro Ile820 825 830Gly Val Lys Phe Glu Lys Phe Ser Asp Cys Asn Asp Phe Ser Tyr Asp835 840 845Leu Thr Leu Ser Tyr Val Pro Asp Leu Ile Arg Asn Asp Pro Lys Cys850 855 860Thr Thr Ala Leu Val Ile Ser Gly Ala Ser Trp Glu Thr Tyr Ala Asn865 870 875 880Asn Leu Ala Arg Gln Ala Leu Gln Val Arg Ala Gly Ser His Tyr Ala885 890 895Phe Ser Pro Met Phe Glu Val Leu Gly Gln Phe ValPhe Glu Val Arg900 905910Gly Ser Ser Arg Ile Tyr Asn Val Asp Leu Gly Gly Lys Phe Gln Phe915 920 925(2)SEQ ID NO5的信息(i)序列特征(A)長(zhǎng)度3052堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO5ATGCGATTTT CGCTCTGCGG ATTTCCTCTA GTTTTTTCTT TAACATTGCT CTCAGTCTTC 60GACACTTCTT TGAGTGCTAC TACGATTTCT TTAACCCCAG AAGATAGTTT TCATGGAGAT 120AGTCAGAATG CAGAACGTTC TTATAATGTT CAAGCTGGGG ATGTCTATAG CCTTACTGGT 180GATGTCTCAA TATCTAACGT CGATAACTCT GCATTAAATA AAGCCTGCTT CAATGTGACC 240TCAGGAAGTG TGACGTTCGC AGGAAATCAT CATGGGTTAT ATTTTAATAA TATTTCCTCA 300GGAACTACAA AGGAAGGGGC TGTACTTTGT TGCCAAGATC CTCAAGCAAC GGCACGTTTT 360TCTGGGTTCT CCACGCTCTC TTTTATTCAG AGCCCCGGAG ATATTAAAGA ACAGGGATGT 420CTCTATTCAA AAAATGCACT TATGCTCTTA AACAATTATG TAGTGCGTTT TGAACAAAAC 480CAAAGTAAGA CTAAAGGCGG AGCTATTAGT GGGGCGAATG TTACTATAGT AGGCAACTAC 540GATTCCGTCT CTTTCTATCA GAATGCAGCC ACTTTTGGAG GTGCTATCCA TTCTTCAGGT 600CCCCTACAGA TTGCAGTAAA TCAGGCAGAG ATAAGATTTG CACAAAATAC TGCCAAGAAT 660GGTTCTGGAG GGGCTTTGTA CTCCGATGGT GATATTGATA TTGATCAGAA TGCTTATGTT 720CTATTTCGAG AAAATGAGGC ATTGACTACT GCTATAGGTA AGGGAGGGGC TGTCTGTTGT 780CTTCCCACTT CAGGAAGTAG TACTCCAGTT CCTATTGTGA CTTTCTCTGA CAATAAACAG 840TTAGTCTTTG AAAGAAACCA TTCCATAATG GGTGGCGGAG CCATTTATGC TAGGAAACTT 900AGCATCTCTT CAGGAGGTCC TACTCTATTT ATCAATAATA TATCATATGC AAATTCGCAA 960AATTTAGGTG GAGCTATTGC CATTGATACT GGAGGGGAGA TCAGTTTATC AGCAGAGAAA 1020GGAACAATTA CATTCCAAGG AAACCGGACG AGCTTACCGT TTTTGAATGG CATCCATCTT 1080TTACAAAATG CTAAATTCCT GAAATTACAG GCGAGAAATG GATGCTCTAT AGAATTTTAT 1140GATCCTATTA CTTCTGAAGC AGATGGGTCT ACCCAATTGA ATATCAACGG AGATCCTAAA 1200AATAAAGAGT ACACAGGGAC CATACTCTTT TCTGGAGAAA AGAGTCTAGC AAACGATCCT 1260AGGGATTTTA AATCTACAAT CCCTCAGAAC GTCAACCTGT CTGCAGGATA CTTAGTTATT 1320AAAGAGGGGG CCGAAGTCAC AGTTTCAAAA TTCACGCAGT CTCCAGGATC GCATTTAGTT 1380TTAGATTTAG GAACCAAACT GATAGCCTCT AAGGAAGACA TTGCCATCAC AGGCCTCGCG 1440ATAGATATAG ATAGCTTAAG CTCATCCTCA ACAGCAGCTG TTATTAAAGC AAACACCGCA 1500AATAAACAGA TATCCGTGAC GGACTCTATA GAACTTATCT CGCCTACTGG CAATGCCTAT 1560GAAGATCTCA GAATGAGAAA TTCACAGACG TTCCCTCTGC TCTCTTTAGA GCCTGGAGCC 1620GGGGGTAGTG TGACTGTAAC TGCTGGAGAT TTCCTACCGG TAAGTCCCCA TTATGGTTTT 1680CAAGGCAATT GGAAATTAGC TTGGACAGGA ACTGGAAACA AAGTTGGAGA ATTCTTCTGG 1740GATAAAATAA ATTATAAGCC TAGACCTGAA AAAGAAGGAA ATTTAGTTCC TAATATCTTG 1800TGGGGGAATG CTGTAAATGT CAGATCCTTA ATGCAGGTTC AAGAGACCCA TGCATCGAGC 1860TTACAGACAG ATCGAGGGCT GTGGATCGAT GGAATTGGGA ATTTCTTCCA TGTATCTGCC 1920TCCGAAGACA ATATAAGGTA CCGTCATAAC AGCGGTGGAT ATGTTCTATC TGTAAATAAT 1980GAGATCACAC CTAAGCACTA TACTTCGATG GCATTTTCCC AACTCTTTAG TAGAGACAAG 2040GACTATGCGG TTTCCAACAA CGAATACAGA ATGTATTTAG GATCGTATCT CTATCAATAT 2100ACAACCTCCC TAGGGAATAT TTTCCGTTAT GCTTCGCGTA ACCCTAATGT AAACGTCGGG 2160ATTCTCTCAA GAAGGTTTCT TCAAAATCCT CTTATGATTT TTCATTTTTT GTGTGCTTAT 2220GGTCATGCCA CCAATGATAT GAAAACAGAC TACGCAAATT TCCCTATGGT GAAAAACAGC 2280TGGAGAAACA ATTGTTGGGC TATAGAGTGC GGAGGGAGCA TGCCTCTATT GGTATTTGAG 2340AACGGAAGAC TTTTCCAAGG TGCCATCCCA TTTATGAAAC TACAATTAGT TTATGCTTAT 2400CAGGGAGATT TCAAAGAGAC GACTGCAGAT GGCCGTAGAT TTAGTAATGG GAGTTTAACA 2460TCGATTTCTG TACCTCTAGG CATACGCTTT GAGAAGCTGG CACTTTCTCA GGATGTACTC 2520TATGACTTTA GTTTCTCCTA TATTCCTGAT ATTTTCCGTA AGGATCCCTC ATGTGAAGCT 2580GCTCTGGTGA TTAGCGGAGA CTCCTGGCTT GTTCCGGCAG CACACGTATC AAGACATGCT 2640TTTGTAGGGA GTGGAACGGG TCGGTATCAC TTTAACGACT ATACTGAGCT CTTATGTCGA 2700GGAAGTATAG AATGCCGCCC CCATGCTAGG AATTATAATA TAAACTGTGG AAGCAAATTT 2760CGTTTTTAGA AGGTTTCCAT TGCCTGTGTG GTTCCGGATC TTAACTATAA ATCCTGGACT 2820ATGGATCATA GGCATTGGGT TTCTCGAACT TGTGTGGAGA ATAACGACAT TTTATATGCA 2880TAACGGAATA CTCGTATCAC CTCAGCCCCT AGAGACATTC TTTAGGGGTT CTTTATTTGT 2940CTAAACTTCG TATTTTATCG AGAATCCTTT ACGTTCTTGG TTTGCTTGTC TCCGAGGAGT 3000TCTCTAACGA ATCATAGGGA TTCCAGGGTT CTGTTCCTTG AGTCCTTTGG CA 3052(2)SEQ ID NO6的信息(i)序列特征(A)長(zhǎng)度922氨基酸
(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO6Met Arg Phe Ser Leu Cys Gly Phe Pro Leu ValPhe Ser Leu Thr Leu1 5 10 15Leu Ser Val Phe Asp Thr Ser Leu Ser Ala Thr Thr Ile Ser Leu Thr20 25 30Pro Glu Asp Ser Phe His Gly Asp Ser Gln Asn Ala Glu Arg Ser Tyr35 40 45Asn Val Gln Ala Gly Asp Val Tyr Ser Leu Thr Gly Asp Val Ser Ile50 55 60Ser Asn Val Asp Asn Ser Ala Leu Asn Lys Ala Cys Phe Asn Val Thr65 70 75 80Ser Gly Ser Val Thr Phe Ala Gly Asn His His Gly Leu Tyr Phe Asn85 90 95Asn Ile Ser Ser Gly Thr Thr Lys Glu Gly Ala Val Leu Cys Cys Gln100 105 110Asp Pro Gln Ala Thr Ala Arg Phe Ser Gly Phe Ser Thr Leu Ser Phe115 120 125Ile Gln Ser Pro Gly Asp Ile Lys Glu Gln Gly Cys Leu Tyr Ser Lys130 135 140Asn Ala Leu Met Leu Leu Asn Asn Tyr Val Val Arg Phe Glu Gln Asn145 150 155 160Gln Ser Lys Thr Lys Gly Gly Ala Ile Ser Gly Ala Asn Val Thr Ile165 170 175Val Gly Asn Tyr Asp Ser Val Ser Phe Tyr Gln Asn Ala Ala Thr Phe180 185 190Gly Gly Ala Ile His Ser Ser Gly Pro Leu Gln Ile Ala Val Asn Gln195 200 205Ala Glu Ile Arg Phe Ala Gln Asn Thr Ala Lys Asn Gly Ser Gly Gly210 215 220Ala Leu Tyr Ser Asp Gly Asp Ile Asp Ile Asp Gln Asn Ala Tyr Val225 230 235 240Leu Phe Arg Glu Asn Glu Ala Leu Thr Thr Ala Ile Gly Lys Gly Gly245 250 255Ala Val Cys Cys Leu Pro Thr Ser Gly Ser Ser Thr Pro Val Pro Ile260 265 270Val Thr Phe Ser Asp Asn Lys Gln Leu Val Phe Glu Arg Asn His Ser275 280 285Ile Met Gly Gly Gly Ala Ile Tyr Ala Arg Lys Leu Ser Ile Ser Ser290 295 300Gly Gly Pro Thr Leu Phe Ile Asn Asn Ile Ser Tyr Ala Asn Ser Gln305 310 315 320Asn Leu Gly Gly Ala Ile Ala Ile Asp Thr Gly Gly Glu Ile Ser Leu325 330 335Ser Ala Glu Lys Gly Thr Ile Thr Phe Gln Gly Asn Arg Thr Ser Leu340 345 350Pro Phe Leu Asn Gly Ile His Leu Leu Gln Asn Ala Lys Phe Leu Lys355 360 365Leu Gln Ala Arg Asn Gly Cys Ser Ile Glu Phe Tyr Asp Pro Ile Thr370 375 380Ser Glu Ala Asp Gly Ser Thr Gln Leu Asn Ile Asn Gly Asp Pro Lys385 390 395 400Asn Lys Glu Tyr Thr Gly Thr Ile Leu Phe Ser Gly Glu Lys Ser Leu405 410 415Ala Asn Asp Pro Arg Asp Phe Lys Ser Thr Ile Pro Gln Asn Val Asn420 425 430Leu Ser Ala Gly Tyr Leu Val Ile Lys Glu Gly Ala Glu Val Thr Val435 440 445Ser Lys Phe Thr Gln Ser Pro Gly Ser His Leu Val Leu Asp Leu Gly450 455 460Thr Lys Leu Ile Ala Ser Lys Glu Asp Ile Ala Ile Thr Gly Leu Ala465 470 475 480Ile Asp Ile Asp Ser Leu Ser Ser Ser Ser Thr Ala Ala Val Ile Lys485 490 495Ala Asn Thr Ala Asn Lys Gln Ile Ser Val Thr Asp Ser Ile Glu Leu500 505 510Ile Ser Pro Thr Gly Asn Ala Tyr Glu Asp Leu Arg Met Arg Asn Ser515 520 525Gln Thr Phe Pro Leu Leu Ser Leu Glu Pro Gly Ala Gly Gly Ser Val530535 540Thr Val Thr Ala Gly Asp Phe Leu Pro Val Ser Pro His Tyr Gly Phe545 550 555 560Gln Gly Asn Trp Lys Leu Ala Trp Thr Gly Thr Gly Asn Lys Val Gly565 570 575Glu Phe Phe Trp Asp Lys Ile Asn Tyr Lys Pro Arg Pro Glu Lys Glu580 585 590Gly Asn Leu Val Pro Asn Ile Leu Trp Gly Asn Ala Val Asn Val Arg595 600 605Ser Leu Met Gln Val Gln Glu Thr His Ala Ser Ser Leu Gln Thr Asp610615 620Arg Gly Leu Trp Ile Asp Gly Ile Gly Asn Phe Phe His ValSer Ala625 630 635640Ser Glu Asp Asn Ile Arg Tyr Arg His Asn Ser Gly Gly Tyr Val Leu645 650 655Ser Val Asn Asn Glu Ile Thr Pro Lys His Tyr Thr Ser Met Ala Phe660 665 670Ser Gln Leu Phe Ser Arg Asp Lys Asp Tyr Ala Val Ser Asn Asn Glu675 680 685Tyr Arg Met Tyr Leu Gly Ser Tyr Leu Tyr Gln Tyr Thr Thr Ser Leu690 695 700Gly Asn Ile Phe Arg Tyr Ala Ser Arg Asn Pro Asn Val Asn Val Gly705 710 715 720Ile Leu Ser Arg Arg Phe Leu Gln Asn Pro Leu Met Ile Phe His Phe725 730 735Leu Cys Ala Tyr Gly His Ala Thr Asn Asp Met Lys Thr Asp Tyr Ala740745 750Asn Phe Pro Met Val Lys Asn Ser Trp Arg Asn Asn Cys Trp Ala Ile755 760 765Glu Cys Gly Gly Ser Met Pro Leu Leu Val Phe Glu Asn Gly Arg Leu770775 780Phe Gln Gly Ala Ile Pro Phe Met Lys Leu Gln Leu Val Tyr Ala Tyr785 790 795 800Gln Gly Asp Phe Lys Glu Thr Thr Ala Asp Gly Arg Arg Phe Ser Asn805 810 815Gly Ser Leu Thr Ser Ile Ser Val Pro Leu Gly Ile Arg Phe Glu Lys820 825 830Leu Ala Leu Ser Gln Asp Val Leu Tyr Asp Phe Ser Phe Ser Tyr Ile835 840 845Pro Asp Ile Phe Arg Lys Asp Pro Ser Cys Glu Ala Ala Leu Val Ile850 855 860Ser Gly Asp Ser Trp Leu Val Pro Ala Ala His Val Ser Arg His Ala865 870 875 880Phe Val Gly Ser Gly Thr Gly Arg Tyr His Phe Asn Asp Tyr Thr Glu885 890 895Leu Leu Cys Arg Gly Ser Ile Glu Cys Arg Pro His Ala Arg Asn Tyr900 905 910Asn Ile Asn Cys Gly Ser Lys Phe Arg Phe915 920(2)SEQ ID NO7的信息(i)序列特征(A)長(zhǎng)度2526堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO7ATGAAGATTC CACTCCGCTT TTTATTGATA TCATTAGTAC CTACGCTTTC TATGTCGAAT 60TTATTAGGAG CTGCTACTAC CGAAGAGCTA TCGGCTAGCA ATAGCTTCGA TGGAACTACA 120TCAACAACAA GCTTTTCTAG TAAAACATCA TCGGCTACAG ATGGCACCAA TTATGTTTTT 180AAAGATTCTG TAGTTATAGA AAATGTACCC AAAACAGGGG AAACTCAGTC TACTAGTTGT 240TTTAAAAATG ACGCTGCAGC TGGAGATCTA AATTTCTTAG GAGGGGGATT TTCTTTCACA 300TTTAGCAATA TCGATGCAAC CACGGCTTCT GGAGCTGCTA TTGGAAGTGA AGCAGCTAAT 360AAGACAGTCA CGTTATCAGG ATTTTCGGCA CTTTCTTTTC TTAAATCCCC AGCAAGTACA 420GTGACTAATG GATTGGGAGC TATCAATGTT AAAGGGAATT TAAGCCTATT GGATAATGAT 480AAGGTATTGA TTCAGGACAA TTTCTCAACA GGAGATGGCG GAGCAATTAA TTGTGCAGGC 540TCCTTGAAGA TCGCAAACAA TAAGTCCCTT TCTTTTATTG GAAATAGTTC TTCAACACGT 600GGCGGAGCGA TTCATACCAA AAACCTCACA CTATCTTCTG GTGGGGAAAC TCTATTTCAG 660GGGAATACAG CGCCTACGGC TGCTGGTAAA GGAGGTGCTA TCGCGATTGC AGACTCTGGC 720ACCCTATCCA TTTCTGGAGA CAGTGGCGAC ATTATCTTTG AAGGCAATAC GATAGGAGCT 780ACAGGAACCG TCTCTCATAG TGCTATTGAT TTAGGAACTA GCGCTAAGAT AACTGCGTTA 840CGTGCTGCGC AAGGACATAC GATATACTTT TATGATCCGA TTACTGTAAC AGGATCGACA 900TCTGTTGCTG ATGCTCTCAA TATTAATAGC CCTGATACTG GAGATAACAA AGAGTATACG 960GGAACCATAG TCTTTTCTGG AGAGAAGCTC ACGGAGGCAG AAGCTAAAGA TGAGAAGAAC 1020CGCACTTCTA AATTACTTCA AAATGTTGCT TTTAAAAATG GGACTGTAGT TTTAAAAGGT 1080GATGTCGTTT TAAGTGCGAA CGGTTTCTCT CAGGATGCAA ACTCTAAGTT GATTATGGAT 1140TTAGGGACGT CGTTGGTTGC AAACACCGAA AGTATCGAGT TAACGAATTT GGAAATTAAT 1200ATAGACTCTC TCAGGAACGG GAAAAAGATA AAACTCAGTG CTGCCACAGC TCAGAAAGAT 1260ATTCGTATAG ATCGTCCTGT TGTACTGGCA ATTAGCGATG AGAGTTTTTA TCAAAATGGC 1320TTTTTGAATG AGGACCATTC CTATGATGGG ATTCTTGAGT TAGATGCTGG GAAAGACATC 1380GTGATTTCTG CAGATTCTCG CAGTATAAAT GCTGTACAAT CTCCGTATGG CTATCAGGGA 1440AAGTGGACAA TCAATTGGTC TACTGATGAT AAGAAAGCTA CGGTTTCTTG GGCAAAGCAA 1500AGTTTTAATC CCACTGCTGA GCAGGAGGCT CCGTTAGTTC CTAATCTTCT TTGGGGTTCT 1560TTTATAGATG TTCGTCCCTT CCAAAATTTT ATAGAGCTAG GTACTGAAGG TGCTCCTTAC 1620GAAAAGAGAT TTTGGGTTGC AGGCATTTCC AATGTTTTGC ATAGGAGCGG TCGTGAAAAT 1680CAAAGGAAAT TCCGTCATGT GAGTGGAGGT GCTGTAGTAG GTGCTAGCAC GAGGATGCCG 1740GGTGGTGATA CCTTGTCTCT GGGTTTTGCT CAGCTCTTTG CGCGTGACAA AGACTACTTT 1800ATGAATACCA ATTTCGCAAA GACCTACGCA GGATCTTTAC GTTTGCAGCA CGATGCTTCC 1860CTATACTCTG TGGTGAGTAT CCTTTTAGGA GAGGGAGGAC TCCGCGAGAT CCTGTTGCCT 1920TATGTTTCCA AGACTCTGCC GTGCTCTTTC TATGGGCAGC TTAGCTACGG CCATACGGAT 1980CATCGCATGA AGACCGAGTC TCTACCCCCC CCCCCCCCGA CGCTCTCGAC GGATCATACT 2040TCTTGGGGAG GATATGTCTG GGCTGGAGAG CTGGGAACTC GAGTTGCTGT TGAAAATACC 2100AGCGGCAGAG GATTTTTCCG AGAGTACACT CCATTTGTAA AAGTCCAAGC TGTTTACTCG 2160CGCCAAGATA GCTTTGTTGA ACTAGGAGCT ATCAGTCGTG ATTTTAGTGA TTCGCATCTT 2220TATAACCTTG CGATTCCTCT TGGAATCAAG TTAGAGAAAC GGTTTGCAGA GCAATATTAT 2280CATGTTGTAG CGATGTATTC TCCAGATGTT TGTCGTAGTA ACCCCAAATG TACGACTACC 2340CTACTTTCCA ACCAAGGGAG TTGGAAGACC AAAGGTTCGA ACTTAGCAAG ACAGGCTGGT 2400ATTGTTCAGG CCTCAGGTTT TCGATGTTTG GGAGCTGCAG CAGAGCTTTT CGGGAACTTT 2460GGCTTTGAAT GGCGGGGATC TTCTCGTAGC TATAATGTAG ATGCGGGTAG CAAAATCAAA 2520TTTTAG2526(2)SEQ ID NO8的信息(i)序列特征(A)長(zhǎng)度841氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO8Met Lys Ile Pro Leu Arg Phe Leu Leu Ile Ser Leu Val Pro Thr Leu1 5 10 15Ser Met Ser Asn Leu Leu Gly Ala Ala Thr Thr Glu Glu Leu Ser Ala20 25 30Ser Asn Ser Phe Asp Gly Thr Thr Ser Thr Thr Ser Phe Ser Ser Lys35 40 45Thr Ser Ser Ala Thr Asp Gly Thr Asn Tyr Val Phe Lys Asp Ser Val50 55 60Val Ile Glu Asn Val Pro Lys Thr Gly Glu Thr Gln Ser Thr Ser Cys65 70 75 80Phe Lys Asn Asp Ala Ala Ala Gly Asp Leu Asn Phe Leu Gly Gly Gly85 90 95Phe Ser Phe Thr Phe Ser Asn Ile Asp Ala Thr Thr Ala Ser Gly Ala100 105 110Ala Ile Gly Ser Glu Ala Ala Asn Lys Thr Val Thr Leu Ser Gly Phe
115 120 125Ser Ala Leu Ser Phe Leu Lys Ser Pro Ala Ser Thr Val Thr Asn Gly130 135140Leu Gly Ala Ile Asn Val Lys Gly Asn Leu Ser Leu Leu Asp Asn Asp145 150 155 160Lys Val Leu Ile Gln Asp Asn Phe Ser Thr Gly Asp Gly Gly Ala Ile165 170 175Asn Cys Ala Gly Ser Leu Lys Ile Ala Asn Asn Lys Ser Leu Ser Phe180 185 190Ile Gly Asn Ser Ser Ser Thr Arg Gly Gly Ala Ile His Thr Lys Asn195 200 205Leu Thr Leu Ser Ser Gly Gly Glu Thr Leu Phe Gln Gly Asn Thr Ala210 215 220Pro Thr Ala Ala Gly Lys Gly Gly Ala Ile Ala Ile Ala Asp Ser Gly225 230 235 240Thr Leu Ser Ile Ser Gly Asp Ser Gly Asp Ile Ile Phe Glu Gly Asn245 250 255Thr Ile Gly Ala Thr Gly Thr Val Ser His Ser Ala Ile Asp Leu Gly260 265 270Thr Ser Ala Lys Ile Thr Ala Leu Arg Ala Ala Gln Gly His Thr Ile275 280 285Tyr Phe Tyr Asp Pro Ile Thr Val Thr Gly Ser Thr Ser Val Ala Asp290 295 300Ala Leu Asn Ile Asn Ser Pro Asp Thr Gly Asp Asn Lys Glu Tyr Thr305 310 315 320Gly Thr Ile Val Phe Ser Gly Glu Lys Leu Thr Glu Ala Glu Ala Lys325 330 335Asp Glu Lys Asn Arg Thr Ser Lys Leu Leu Gln Asn Val Ala Phe Lys340 345 350Asn Gly Thr Val Val Leu Lys Gly Asp Val Val Leu Ser Ala Asn Gly355 360 365Phe Ser Gln Asp Ala Asn Ser Lys Leu Ile Met Asp Leu Gly Thr Ser370 375 380Leu Val Ala Asn Thr Glu Ser Ile Glu Leu Thr Asn Leu Glu Ile Asn385 390 395 400Ile Asp Ser Leu Arg Asn Gly Lys Lys Ile Lys Leu Ser Ala Ala Thr405 410 415Ala Gln Lys Asp Ile Arg Ile Asp Arg Pro Val Val Leu Ala Ile Ser420 425 430Asp Glu Ser Phe Tyr Gln Asn Gly Phe Leu Asn Glu Asp His Ser Tyr435 440 445Asp Gly Ile Leu Glu Leu Asp Ala Gly Lys Asp Ile Val Ile Ser Ala450 455 460Asp Ser Arg Ser Ile Asn Ala Val Gln Ser Pro Tyr Gly Tyr Gln Gly465 470 475 480Lys Trp Thr Ile Asn Trp Ser Thr Asp Asp Lys Lys Ala Thr Val Ser485 490 495Trp Ala Lys Gln Ser Phe Asn Pro Thr Ala Glu Gln Glu Ala Pro Leu500 505 510Val Pro Asn Leu Leu Trp Gly Ser Phe Ile Asp Val Arg Pro Phe Gln515 520 525Asn Phe Ile Glu Leu Gly Thr Glu Gly Ala Pro Tyr Glu Lys Arg Phe
530 535 540Trp Val Ala Gly Ile Ser Asn Val Leu His Arg Ser Gly Arg Glu Asn545 550 555 560Gln Arg Lys Phe Arg His Val Ser Gly Gly Ala Val Val Gly Ala Ser565 570 575Thr Arg Met Pro Gly Gly Asp Thr Leu Ser Leu Gly Phe Ala Gln Leu580 585 590Phe Ala Arg Asp Lys Asp Tyr Phe Met Asn Thr Asn Phe Ala Lys Thr595 600 605Tyr Ala Gly Ser Leu Arg Leu Gln His Asp Ala Ser Leu Tyr Ser Val610 615 620Val Ser Ile Leu Leu Gly Glu Gly Gly Leu Arg Glu Ile Leu Leu Pro625 630 635 640Tyr Val Ser Lys Thr Leu Pro Cys Ser Phe Tyr Gly Gln Leu Ser Tyr645 650 655Gly His Thr Asp His Arg Met Lys Thr Glu Ser Leu Pro Pro Pro Pro660 665 670Pro Thr Leu Ser Thr Asp His Thr Ser Trp Gly Gly Tyr Val Trp Ala675 680 685Gly Glu Leu Gly Thr Arg Val Ala Val Glu Asn Thr Ser Gly Arg Gly690 695 700Phe Phe Arg Glu Tyr Thr Pro Phe Val Lys Val Gln Ala Val Tyr Ser705 710 715 720Arg Gln Asp Ser Phe Val Glu Leu Gly Ala Ile Ser Arg Asp Phe Ser725 730 735Asp Ser His Leu Tyr Asn Leu Ala Ile Pro Leu Gly Ile Lys Leu Glu740 745 750Lys Arg Phe Ala Glu Gln Tyr Tyr His Val Val Ala Met Tyr Ser Pro755 760 765Asp Val Cys Arg Ser Asn Pro Lys Cys Thr Thr Thr Leu Leu Ser Asn770 775 780Gln Gly Ser Trp Lys Thr Lys Gly Ser Asn Leu Ala Arg Gln Ala Gly785 790 795 800Ile Val Gln Ala Ser Gly Phe Arg Ser Leu Gly Ala Ala Ala Glu Leu805 810 815Phe Gly Asn Phe Gly Phe Glu Trp Arg Gly Ser Ser Arg Ser Tyr Asn820 825 830Val Asp Ala Gly Ser Lys Ile Lys Phe835 840(2)SEQ ID NO9的信息(i)序列特征(A)長(zhǎng)度2787堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO9ATGAAGTCTT CTTTCCCCAA GTTTGTATTT TCTACATTTG CTATTTTCCC TTTGTCTATG 60ATTGCTACCG AGACAGTTTT GGATTCAAGT GCGAGTTTCG ATGGGAATAA AAATGGTAAT 120TTTTCAGTTC GTGAGAGTCA GGAAGATGCT GGAACTACCT ACCTATTTAA GGGAAATGTC 180ACTCTAGAAA ATATTCCTGG AACAGGCACA GCAATCACAA AAAGCTGTTT TAACAACACT 240AAGGGCGATT TGACTTTCAC AGGTAACGGG AACTCTCTAT TGTTCCAAAC GGTGGATGCA 300GGGACTGTAG CAGGGGCTGC TGTTAACAGC AGCGTGGTAG ATAAATCTAC CACGTTTATA 360GGGTTTTCTT CGCTATCTTT TATTGCGTCT CCTGGAAGTT CGATAACTAC CGGCAAAGGA 420GCCGTTAGCT GCTCTACGGG TAGCTTGAAG TTTGACAAAA ATGTCAGTTT GCTCTTCAGC 480AAAAACTTTT CAACGGATAA TGGCGGTGCT ATCACCGCAA AAACTCTTTC ATTAACAGGG 540ACTACAATGT CAGCTCTGTT TTCTGAAAAT ACCTCCTCAA AGAAAGGCGG AGCCATTCAG 600ACTTCCGATG CCCTTACCAT TACTGGAAAC CAAGGGGAAG TCTCTTTTTC TGACAATACT 660TCTTCGGATT CTGGAGCTGC AATTTTTACA GAAGCCTCGG TGACTATTTC TAATAATGCT 720AAAGTTTCCT TTATTGACAA TAAGGTCACA GGAGCGAGCT CCTCAACAAC GGGGGATATG 780TCAGGAGGTG CTATCTGTGC TTATAAAACT AGTACAGATA CTAAGGTCAC CCTCACTGGA 840AATCAGATGT TACTCTTCAG CAACAATACA TCGACAACAG CGGGAGGAGC TATCTATGTG 900AAAAAGCTCG AACTGGCTTC CGGAGGACTT ACCCTATTCA GTAGAAATAG TGTCAATGGA 960GGTACAGCTC CTAAAGGTGG AGCCATAGCT ATCGAAGATA GTGGGGAATT GAGTTTATCC 1020GCCGATAGTG GTGACATTGT CTTTTTAGGG AATACAGTCA CTTCTACTAC TCCTGGGACG 1080AATAGAAGTA GTATCGACTT AGGAACGAGT GCAAAGATGA CAGCTTTGCG TTCTGCTGCT 1140GGTAGAGCCA TCTACTTCTA TGATCCCATA ACTACAGGAT CTTCCACAAC AGTTACAGAT 1200GTCTTAAAAG TTAATGAGAC TCCGGCAGAT TCTGCACTAC AATATACAGG GAACATCATC 1260TTCACAGGAG AAAAGTTATC AGAGACAGAG GCCGCAGATT CTAAAAATCT TACTTCGAAG 1320CTACTACAGC CTGTAACTCT TTCAGGAGGT ACTCTATCTT TAAAACATGG AGTGACTCTG 1380CAGACTCAGG CATTCACTCA ACAGGCAGAT TCTCGTCTCG AAATGGACGT AGGAACTACT 1440CTAGAACCTG CTGATACTAG CACCATAAAC AATTTGGTCA TTAACATCAG TTCTATAGAC 1500GGTGCAAAGA AGGCAAAAAT AGAAACCAAA GCTACGTCAA AAAATCTGAC TTTATCTGGA 1560ACCATCACTT TATTGGACCC GACGGGCACG TTTTATGAAA ATCATAGTTT AAGAAATCCT 1620CAGTCCTACG ACATCTTAGA GCTCAAAGCT TCTGGAACTG TAACAAGCAC CGCAGTGACT 1680CCAGATCCTA TAATGGGTGA GAAATTCCAT TACGGCTATC AGGGAACTTG GGGCCCAATT 1740GTTTGGGGGA CAGGGGCTTC TACGACTGCA ACCTTCAACT GGACTAAAAC TGGCTATATT 1800CCTAATCCCG AGCGTATCGG CTCTTTAGTC CCTAATAGCT TATGGAATGC ATTTATAGAT 1860ATTAGCTCTC TCCATTATCT TATGGAGACT GCAAACGAAG GGTTGCAGGG AGACCGTGCT 1920TTTTGGTGTG CTGGATTATC TAACTTCTTC CATAAGGATA GTACAAAAAC ACGACGCGGG 1980TTTCGCCATT TGAGTGGCGG TTATGTCATA GGAGGAAACC TACATACTTG TTCAGATAAG 2040ATTCTTAGTG CTGCATTTTG TCAGCTCTTT GGAAGAGATA GAGACTACTT TGTAGCTAAG 2100AATCAAGGTA CAGTCTACGG AGGAACTCTC TATTACCAGC ACAACGAAAC CTATATCTCT 2160CTTCCTTGCA AACTACGGCC TTGTTCGTTG TCTTATGTTC CTACAGAGAT TCCTGTTCTC 2220TTTTCAGGAA ACCTTAGCTA CACCCATACG GATAACGATC TGAAAACCAA GTATACAACA 2280TATCCTACTG TTAAAGGAAG CTGGGGGAAT GATAGTTTCG CTTTAGAATT CGGTGGAAGA 2340GCTCCGATTT GCTTAGATGA AAGTGCTCTA TTTGAGCAGT ACATGCCCTT CATGAAATTG 2400CAGTTTGTCT ATGCACATCA GGAAGGTTTT AAAGAACAGG GAACAGAAGC TCGTGAATTT 2460GGAAGTAGCC GTCTTGTGAA TCTTGCCTTA CCTATCGGGA TCCGATTTGA TAAGGAATCA 2520GACTGCCAAG ATGCAACGTA CAATCTAACT CTTGGTTATA CTGTGGATCT TGTTCGTAGT 2580AACCCCGACT GTACGACAAC ACTGCGAATT AGCGGTGATT CTTGGAAAAC CTTCGGTACG 2640AATTTGGCAA GACAAGCTTT AGTCCTTCGT GCAGGGAACC ATTTTTGCTT TAACTCAAAT 2700TTTGAAGCCT TTAGCCAATT TTCTTTTGAA TTGCGTGGGT CATCTCGCAA TTACAATGTA 2760GACTTAGGAG CAAAATACCA ATTCTAA 2787(2)SEQ ID NO10的信息(i)序列特征(A)長(zhǎng)度928氨基酸
(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO10Met Lys Ser Ser Phe Pro Lys Phe Val Phe Ser Thr Phe Ala Ile Phe1 5 10 15Pro Leu Ser Met Ile Ala Thr Glu Thr Val Leu Asp Ser Ser Ala Ser20 25 30Phe Asp Gly Asn Lys Asn Gly Asn Phe Ser Val Arg Glu Ser Gln Glu35 40 45Asp Ala Gly Thr Thr Tyr Leu Phe Lys Gly Asn Val Thr Leu Glu Asn50 55 60Ile Pro Gly Thr Gly Thr Ala Ile Thr Lys Ser Cys Phe Asn Asn Thr65 70 75 80Lys Gly Asp Leu Thr Phe Thr Gly Asn Gly Asn Ser Leu Leu Phe Gln85 90 95Thr Val Asp Ala Gly Thr Val Ala Gly Ala Ala Val Asn Ser Ser Val100 105 110Val Asp Lys Ser Thr Thr Phe Ile Gly Phe Ser Ser Leu Ser Phe Ile115 120 125Ala Ser Pro Gly Ser Ser Ile Thr Thr Gly Lys Gly Ala Val Ser Cys130 135 140Ser Thr Gly Ser Leu Lys Phe Asp Lys Asn Val Ser Leu Leu Phe Ser145 150 155 160Lys Asn Phe Ser Thr Asp Asn Gly Gly Ala Ile Thr Ala Lys Thr Leu165 170 175Ser Leu Thr Gly Thr Thr Met Ser Ala Leu Phe Ser Glu Asn Thr Ser180 185 190Ser Lys Lys Gly Gly Ala Ile Gln Thr Ser Asp Ala Leu Thr Ile Thr195 200 205Gly Asn Gln Gly Glu Val Ser Phe Ser Asp Asn Thr Ser Ser Asp Ser210 215 220Gly Ala Ala Ile Phe Thr Glu Ala Ser Val Thr Ile Ser Asn Asn Ala225 230 235 240Lys Val Ser Phe Ile Asp Asn Lys Val Thr Gly Ala Ser Ser Ser Thr245 250 255Thr Gly Asp Met Ser Gly Gly Ala Ile Cys Ala Tyr Lys Thr Ser Thr260 265 270Asp Thr Lys Val Thr Leu Thr Gly Asn Gln Met Leu Leu Phe Ser Asn275 280 285Asn Thr Ser Thr Thr Ala Gly Gly Ala Ile Tyr Val Lys Lys Leu Glu290295 300Leu Ala Ser Gly Gly Leu Thr Leu Phe Ser Arg Asn Ser Val Asn Gly305 310 315 320Gly Thr Ala Pro Lys Gly Gly Ala Ile Ala Ile Glu Asp Ser Gly Glu325 330 335Leu Ser Leu Ser Ala Asp Ser Gly Asp Ile Val Phe Leu Gly Asn Thr340 345 350Val Thr Ser Thr Thr Pro Gly Thr Asn Arg Ser Ser Ile Asp Leu Gly355 360 365Thr Ser Ala Lys Met Thr Ala Leu Arg Ser Ala Ala Gly Arg Ala Ile370 375 380Tyr Phe Tyr Asp Pro Ile Thr Thr Gly Ser Ser Thr Thr Val Thr Asp385 390 395 400Val Leu Lys Val Asn Glu Thr Pro Ala Asp Ser Ala Leu Gln Tyr Thr405 410 415Gly Asn Ile Ile Phe Thr Gly Glu Lys Leu Ser Glu Thr Glu Ala Ala420 425 430AspSer Lys Asn Leu Thr Ser Lys Leu Leu Gln Pro Val Thr Leu Ser435 440 445Gly Gly Thr Leu Ser Leu Lys His Gly Val Thr Leu Gln Thr Gln Ala450 455 460Phe Thr Gln Gln Ala Asp Ser Arg Leu Glu Met Asp Val Gly Thr Thr465 470 475 480Leu Glu Pro Ala Asp Thr Ser Thr Ile Asn Asn Leu Val Ile Asn Ile485 490 495Ser Ser Ile Asp Gly Ala Lys Lys Ala Lys Ile Glu Thr Lys Ala Thr500 505 510Ser Lys Asn Leu Thr Leu Ser Gly Thr Ile Thr Leu Leu Asp Pro Thr515 520 525Gly Thr Phe Tyr Glu Asn His Ser Leu Arg Asn Pro Gln Ser Tyr Asp530 535 540Ile Leu Glu Leu Lys Ala Ser Gly Thr Val Thr Ser Thr Ala Val Thr545 550 555 560Pro Asp Pro Ile Met Gly Glu Lys Phe His Tyr Gly Tyr Gln Gly Thr565 570 575Trp Gly Pro Ile Val Trp Gly Thr Gly Ala Ser Thr Thr Ala Thr Phe580 585 590Asn Trp Thr Lys Thr Gly Tyr Ile Pro Asn Pro Glu Arg Ile Gly Ser595 600 605Leu Val Pro Asn Ser Leu Trp Asn Ala Phe Ile Asp Ile Ser Ser Leu610 615 620His Tyr Leu Met Glu Thr Ala Asn Glu Gly Leu Gln Gly Asp Arg Ala625 630 635 640Phe Trp Cys Ala Gly Leu Ser Asn Phe Phe His Lys Asp Ser Thr Lys645 650 655Thr Arg Arg Gly Phe Arg His Leu Ser Gly Gly Tyr Val Ile Gly Gly660 665 670Asn Leu His Thr Cys Ser Asp Lys Ile Leu Ser Ala Ala Phe Cys Gln675 680 685Leu Phe Gly Arg Asp Arg Asp Tyr Phe Val Ala Lys Asn Gln Gly Thr690 695 700Val Tyr Gly Gly Thr Leu Tyr Tyr Gln His Asn Glu Thr Tyr Ile Ser705 710 715 720Leu Pro Cys Lys Leu Arg Pro Cys Ser Leu Ser Tyr Val Pro Thr Glu725 730 735Ile Pro Val Leu Phe Ser Gly Asn Leu Ser Tyr Thr His Thr Asp Asn740 745 750Asp Leu Lys Thr Lys Tyr Thr Thr Tyr Pro Thr Val Lys Gly Ser Trp755 760 765Gly Asn Asp Ser Phe Ala Leu Glu Phe Gly Gly Arg Ala Pro Ile Cys770 775 780Leu Asp Glu Ser Ala Leu Phe Glu Gln Tyr Met Pro Phe Met Lys Leu785 790 795 800Gln Phe Val Tyr Ala His Gln Glu Gly Phe Lys Glu Gln Gly Thr Glu805 810 815Ala Arg Glu Phe Gly Ser Ser Arg Leu Val Asn Leu Ala Leu Pro Ile820 825 830Gly Ile Arg Phe Asp Lys Glu Ser Asp Cys Gln Asp Ala Thr Tyr Asn835 840 845Leu Thr Leu Gly Tyr Thr Val Asp Leu ValArg Ser Asn Pro Asp Cys850 855860Thr Thr Thr Leu Arg Ile Ser Gly Asp Ser Trp Lys Thr Phe Gly Thr865 870 875 880Asn Leu Ala Arg Gln Ala Leu Val Leu Arg Ala Gly Asn His Phe Cys885 890 895Phe Asn Ser Asn Phe Glu Ala Phe Ser Gln Phe Ser Phe Glu Leu Arg900 905 910Gly Ser Ser Arg Asn Tyr Asn Val Asp Leu Gly Ala Lys Tyr Gln Phe915 920 925(2)SEQ ID NO11的信息(i)序列特征(A)長(zhǎng)度2757堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO11ATGAGATCGT CTTTTTCCTT GTTATTAATA TCTTCATCTC TAGCCTTTCC TCTCTTAATG 60AGTGTTTCTG CAGATGCTGC CGATCTCACA TTAGGGAGTC GTGACAGTTA TAATGGTGAT 120ACAAGCACCA CAGAATTTAC TCCTAAAGCG GCAACTTCTG ATGCTAGTGG CACGACCTAT 180ATTCTCGATG GGGATGTCTC GATAAGCCAA GCAGGGAAAC AAACGAGCTT AACCACAAGT 240TGTTTTTCTA ACACTGCAGG AAATCTTACC TTCTTAGGGA ACGGATTTTC TCTTCATTTT 300GACAATATTA TTTCGTCTAC TGTTGCAGGT GTTGTTGTTA GCAATACAGC AGCTTCTGGG 360ATTACGAAAT TCTCAGGATT TTCAACTCTT CGGATGCTTG CAGCTCCTAG GACCACAGGT 420AAAGGAGCCA TTAAAATTAC CGATGGTCTG GTGTTTGAGA GTATAGGGAA TCTTGACCAA 480AATGAAAATG CCTCTAGTGA AAATGGGGGA GCCATCAATA CGAAGACTTT GTCTTTGACT 540GGGAGTACGC GGTTTGTAGC GTTCCTTGGC AATAGCTCGT CGCAACAAGG GGGAGCGATC 600TATGCTTCTG GTGACTCTGT GATTTCTGAG AATGCAGGAA TCTTGAGCTT CGGAAACAAC 660AGTGCGACAA CATCAGGAGG CGCGATCTCT GCTGAAGGGA ACCTTGTGAT CTCCAATAAC 720CAAAATATCT TTTTCGATGG CTGCAAAGCA ACTACAAATG GCGGAGCTAT TGATTGTAAC 780AAAGCAGGGG CGAACCCAGA CCCTATCTTG ACTCTTTCAG GAAATGAGAG CCTGCATTTT 840CTGAATAACA CAGCAGGAAA TAGTGGAGGT GCGATTTATA CCAAAAAATT GGTGTTATCC 900TCAGGACGAG GAGGAGTGTT ATTTTCTAAC AACAAAGCTG CGAATGCTAC TCCTAAAGGA 960GGGGCAATTG CGATTCTAGA TTCTGGAGAG ATTAGCATTT CTGCAGATCT CGGCAATATC 1020ATTTTCGAGG GCAATACTAC GAGCACTACA GGAAGTCCTG CGAGTGTGAC CAGAAATGCT 1080ATAGATCTTG CATCGAATGC AAAATTTTTA AATCTCCGAG CGACTCGGGG AAATAAAGTT 1140ATTTTCTATG ATCCTATCAC GAGCTCAGGA GCTACTGATA AGCTCTCTTT GAATAAAGCT 1200GACGCAGGAT CTGGAAATAC CTATGAAGGC TACATCGTTT TCTCTGGAGA GAAACTCTCA 1260GAAGAGGAAC TTAAGAAACC TGACAATCTG AAGTCTACAT TTACACAGGC TGTAGAGCTT 1320GCTGCAGGTG CCTTAGTATT GAAAGATGGA GTGACTGTAG TTGCAAATAC TATAACGCAG 1380GTCGAGGGAT CGAAAGTCGT TATGGATGGA GGGACTACTT TTGAGGCAAG CGCTGAGGGG 1440GTCACTCTCA ATGGCCTAGC CATTAATATA GATTCCTTAG ATGGGACAAA TAAAGCTATC 1500ATTAAGGCGA CGGCAGCAAG TAAGGATGTT GCCTTATCAG GGCCTATCAT GCTTGTAGAT 1560GCTCAGGGGA ACTATTATGA GCATCATAAT CTCAGTCAAC AGCAGGTCTT TCCTTTAATA 1620GAGCTTTCTG CACAAGGAAC GATGACTACT ACAGATATCC CCGATACCCC AATTCTAAAT 1680ACTACGAATC ACTATGGGTA TCAAGGAACT GGAATAATTG TTTGGGTCGA CGATGCAACT 1740GCAAAAACAA AAAATGCTAC CTTAACTTGG ACTAAAACAG GATACAAGCC GAATCCAGAA 1800CGTCAGGGAC CTTTGGTTCC TAATAGCCTG TGGGGTTCTT TTGTCGATGT CCGCTCCATT 1860CAGAGCCTCA TGGACCGGAG CACAAGTTCG TTATCTTCGT CAACAAATTT GTGGGTATCA 1920GGAATCGCGG ACTTTTTGCA TGAAGATCAG AAAGGAAACC AACGTAGTTA TCGTCATTCT 1980AGCGCGGGTT ATGCATTAGG AGGAGGATTC TTCACGGCTT CTGAAAATTT CTTTAATTTT 2040GCTTTTTGTC AGCTTTTTGG CTACGACAAG GACCATCTTG TGGCTAAGAA CCATACCCAT 2100GTATATGCAG GGGCAATGAG TTACCGACAC CTCGGAGAGT CTAAGACCCT CGCTAAGATT 2160TTGTCAGGAA ATTCTGACTC CCTACCTTTT GTCTTCAATG CTCGGTTTGC TTATGGCCAT 2220ACCGACAATA ACATGACCAC AAAGTACACT GGCTATTCTC CTGTTAAGGG AAGCTGGGGA 2280AATGATGCCT TCGGTATAGA ATGTGGAGGA GCTATCCCGG TAGTTGCTTC AGGACGTCGG 2340TCTTGGGTGG ATACCCACAC GCCATTTCTA AACCTAGAGA TGATCTATGC ACATCAGAAT 2400GACTTTAAGG AAAACGGCAC AGAAGGCCGT TCTTTCCAAA GTGAAGACCT CTTCAATCTA 2460GCGGTTCCTG TAGGGATAAA ATTTGAGAAA TTCTCCGATA AGTCTACGTA TGATCTCTCC 2520ATAGCTTACG TTCCCGATGT GATTCGTAAT GATCCAGGCT GCACGACAAC TCTTATGGTT 2580TCTGGGGATT CTTGGTCGAC ATGTGGTACA AGCTTGTCTA GACAAGCTCT TCTTGTACGT 2640GCTGGAAATC ATCATGCCTT TGCTTCAAAC TTTGAAGTTT TCAGTCAGTT TGAAGTCGAG 2700TTGCGAGGTT CTTCTCGTAG CTATGCTATC GATCTTGGAG GAAGATTCGG ATTTTAA2757(2)SEQ ID NO12的信息(i)序列特征(A)長(zhǎng)度918氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO12Met Arg Ser Ser Phe Ser Leu Leu Leu Ile Ser Ser Ser Leu Ala Phe1 5 10 15Pro Leu Leu Met Ser Val Ser Ala Asp Ala Ala Asp Leu Thr Leu Gly20 25 30Ser Arg Asp Ser Tyr Asn Gly Asp Thr Ser Thr Thr Glu Phe Thr Pro35 40 45Lys Ala Ala Thr Ser Asp Ala Ser Gly Thr Thr Tyr Ile Leu Asp Gly50 55 60Asp Val Ser Ile Ser Gln Ala Gly Lys Gln Thr Ser Leu Thr Thr Ser65 70 75 80Cys Phe Ser Asn Thr Ala Gly Asn Leu Thr Phe Leu Gly Asn Gly Phe85 90 95Ser Leu His Phe Asp Asn Ile Ile Ser Ser Thr Val Ala Gly Val Val100 105 110Val Ser Asn Thr Ala Ala Ser Gly Ile Thr Lys Phe Ser Gly Phe Ser115 120 125Thr Leu Arg Met Leu Ala Ala Pro Arg Thr Thr Gly Lys Gly Ala Ile130 135 140Lys Ile Thr Asp Gly Leu Val Phe Glu Ser Ile Gly Asn Leu Asp Gln145 150 155 160Asn Glu Asn Ala Ser Ser Glu Asn Gly Gly Ala Ile Asn Thr Lys Thr165 170 175Leu Ser Leu Thr Gly Ser Thr Arg Phe Val Ala Phe Leu Gly Asn Ser180 185 190Ser Ser Gln Gln Gly Gly Ala Ile Tyr Ala Ser Gly Asp Ser Val Ile195 200 205Ser Glu Asn Ala Gly Ile Leu Ser Phe Gly Asn Asn Ser Ala Thr Thr210 215 220Ser Gly Gly Ala Ile Ser Ala Glu Gly Asn Leu Val Ile Ser Asn Asn225 230 235 240Gln Asn Ile Phe Phe Asp Gly Cys Lys Ala Thr Thr Asn Gly Gly Ala245 250 255Ile Asp Cys Asn Lys Ala Gly Ala Asn Pro Asp Pro Ile Leu Thr Leu260 265 270Ser Gly Asn Glu Ser Leu His Phe Leu Asn Asn Thr Ala Gly Asn Ser275 280 285Gly Gly Ala Ile Tyr Thr Lys Lys Leu Val Leu Ser Ser Gly Arg Gly290 295 300Gly Val Leu Phe Ser Asn Asn Lys Ala Ala Asn Ala Thr Pro Lys Gly305 310 315 320Gly Ala Ile Ala Ile Leu Asp Ser Gly Glu Ile Ser Ile Ser Ala Asp325 330 335Leu Gly Asn Ile Ile Phe Glu Gly Asn Thr Thr Ser Thr Thr Gly Ser340 345 350Pro Ala Ser Val Thr Arg Asn Ala Ile Asp Leu Ala Ser Asn Ala Lys355 360 365Phe Leu Asn Leu Arg Ala Thr Arg Gly Asn Lys Val Ile Phe Tyr Asp370 375 380Pro Ile Thr Ser Ser Gly Ala Thr Asp Lys Leu Ser Leu Asn Lys Ala385 390 395 400Asp Ala Gly Ser Gly Asn Thr Tyr Glu Gly Tyr Ile Val Phe Ser Gly405 410 415Glu Lys Leu Ser Glu Glu Glu Leu Lys Lys Pro Asp Asn Leu Lys Ser420 425 430Thr Phe Thr Gln Ala Val Glu Leu Ala Ala Gly Ala Leu Val Leu Lys435 440 445Asp Gly Val Thr Val Val Ala Asn Thr Ile Thr Gln Val Glu Gly Ser450 455 460Lys Val Val Met Asp Gly Gly Thr Thr Phe Glu Ala Ser Ala Glu Gly465 470 475 480Val Thr Leu Asn Gly Leu Ala Ile Asn Ile Asp Ser Leu Asp Gly Thr485 490 495Asn Lys Ala Ile Ile Lys Ala Thr Ala Ala Ser Lys Asp Val Ala Leu500 505 510Ser Gly Pro Ile Met Leu Val Asp Ala Gln Gly Asn Tyr Tyr Glu His515 520 525His Asn Leu Ser Gln Gln Gln Val Phe Pro Leu Ile Glu Leu Ser Ala530 535 540Gln Gly Thr Met Thr Thr Thr Asp Ile Pro Asp Thr Pro Ile Leu Asn545 550 555 560Thr Thr Asn His Tyr Gly Tyr Gln Gly Thr Gly Ile Ile Val Trp Val565 570 575Asp Asp Ala Thr Ala Lys Thr Lys Asn Ala Thr Leu Thr Trp Thr Lys580 585 590Thr Gly Tyr Lys Pro Asn Pro Glu Arg Gln Gly Pro Leu Val Pro Asn595 600 605Ser Leu Trp Gly Ser Phe Val Asp Val Arg Ser Ile Gln Ser Leu Met610 615 620Asp Arg Ser Thr Ser Ser Leu Ser Ser Ser Thr Asn Leu Trp Val Ser625 630 635 640Gly Ile Ala Asp Phe Leu His Glu Asp Gln Lys Gly Asn Gln Arg Ser645 650 655Tyr Arg His Ser Ser Ala Gly Tyr Ala Leu Gly Gly Gly Phe Phe Thr660 665 670Ala Ser Glu Asn Phe Phe Asn Phe Ala Phe Cys Gln Leu Phe Gly Tyr675 680 685Asp Lys Asp His Leu Val Ala Lys Asn His Thr His Val Tyr Ala Gly690 695 700AIa Met Ser Tyr Arg His Leu Gly Glu Ser Lys Thr Leu Ala Lys Ile705 710 715 720Leu Ser Gly Asn Ser Asp Ser Leu Pro Phe Val Phe Asn Ala Arg Phe725 730 735Ala Tyr Gly His Thr Asp Asn Asn Met Thr Thr Lys Tyr Thr Gly Tyr740 745 750Ser Pro Val Lys Gly Ser Trp Gly Asn Asp Ala Phe Gly Ile Glu Cys755 760 765Gly Gly Ala Ile Pro Val Val Ala Ser Gly Arg Arg Ser Trp Val Asp770 775 780Thr His Thr Pro Phe Leu Asn Leu Glu Met Ile Tyr Ala His Gln Asn785 790 795 800Asp Phe Lys Glu Asn Gly Thr Glu Gly Arg Ser Phe Gln Ser Glu Asp805 810 815Leu Phe Asn Leu Ala Val Pro Val Gly Ile Lys Phe Glu Lys Phe Ser820 825 830Asp Lys Ser Thr Tyr Asp Leu Ser Ile Ala Tyr Val Pro Asp Val Ile835 840 845Arg Asn Asp Pro Gly Cys Thr Thr Thr Leu Met Val Ser Gly Asp Ser850 855 860Trp Ser Thr Cys Gly Thr Ser Leu Ser Arg Gln Ala Leu Leu Val Arg865 870 875 880Ala Gly Asn His His Ala Phe Ala Ser Asn Phe Glu Val Phe Ser Gln885 890 895Phe Glu Val Glu Leu Arg Gly Ser Ser Arg Ser Tyr Ala Ile Asp Leu900 905 910Gly Gly Arg Phe Gly Phe915
(2)SEQ ID NO13的信息(i)序列特征(A)長(zhǎng)度2787堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO13ATGAAATCCT CTCTTCATTG GTTTGTAATC TCGTCATCTT TAGCACTTCC CTTGTCACTA 60AATTTCTCTG CGTTTGCTGC TGTTGTTGAA ATCAATCTAG GACCTACCAA TAGCTTCTCT 120GGACCAGGAA CCTACACTCC TCCAGCCCAA ACAACAAATG CAGATGGAAC TATCTATAAT 180CTAACAGGGG ATGTCTCAAT CACCAATGCA GGATCTCCGA CAGCTCTAAC CGCTTCCTGC 240TTTAAAGAAA CTACTGGGAA TCTTTCTTTC CAAGGCCACG GCTACCAATT TCTCCTACAA 300AATATCGATG CGGGAGCGAA CTGTACCTTT ACCAATACAG CTGCAAATAA GCTTCTCTCC 360TTTTCAGGAT TCTCCTATTT GTCACTAATA CAAACCACGA ATGCTACCAC AGGAACAGGA 420GCCATCAAGT CCACAGGAGC TTGTTCTATT CAGTCGAACT ATAGTTGCTA CTTTGGCCAA 480AACTTTTCTA ATGACAATGG AGGCGCCCTC CAAGGCAGCT CTATCAGTCT ATCGCTAAAC 540CCCAACCTAA CGTTTGCCAA AAACAAAGCA ACGCAAAAAG GGGGTGCCCT CTATTCCACG 600GGAGGGATTA CAATTAACAA TACGTTAAAC TCAGCATCAT TTTCTGAAAA TACCGCGGCG 660AACAATGGCG GAGCCATTTA CACGGAAGCT AGCAGTTTTA TTAGCAGCAA CAAAGCAATT 720AGCTTTATAA ACAATAGTGT GACCGCAACC TCAGCTACAG GGGGAGCCAT TTACTGTAGT 780AGTACATCAG CCCCCAAACC AGTCTTAACT CTATCAGACA ACGGGGAACT GAACTTTATA 840GGAAATACAG CAATTACTAG TGGTGGGGCG ATTTATACTG ACAATCTAGT TCTTTCTTCT 900GGAGGACCTA CGCTTTTTAA AAACAACTCT GCTATAGATA CTGCAGCTCC CTTAGGAGGA 960GCAATTGCGA TTGCTGACTC TGGATCTTTG AGTCTTTCGG CTCTTGGTGG AGACATCACT 1020TTTGAAGGAA ACACAGTAGT CAAAGGAGCT TCTTCGAGTC AGACCACTAC CAGAAATTCT 1080ATTAACATCG GAAACACCAA TGCTAAGATT GTACAGCTGC GAGCCTCTCA AGGCAATACT 1140ATCTACTTCT ATGATCCTAT AACAACTAAC CATACTGCAG CTCTCTCAGA TGCTCTAAAC 1200TTAAATGGTC CTGACCTTGC AGGGAATGCT GCATATCAAG GAACCATCGT ATTTTCTGGA 1260GAGAAGCTCT CGGAAGCAGA AGCTGCAGAA GCTGATAATC TCAAATCTAC AATTCAGCAA 1320CCTCTAACTC TTGCGGGAGG GCAACTCTCT CTTAAATCAG GAGTCACTCT AGTTGCTAAG 1380TCCTTTTCGC AATCTCCGGG CTCTACCCTC CTCATGGATG CAGGGACCAC ATTAGAAACC 1440GCTGATGGGA TCACTATCAA TAATCTTGTT CTCAATGTAG ATTCCTTAAA AGAGACCAAG 1500AAGGCTACGC TAAAAGCAAC ACAAGCAAGT CAGACAGTCA CTTTATCTGG ATCGCTCTCT 1560CTTGTAGATC CTTCTGGAAA TGTCTACGAA GATGTCTCTT GGAATAACCC TCAAGTCTTT 1620TCTTGTCTCA CTCTTACTGC TGACGACCCC GCGAATATTC ACATCACAGA CTTAGCTGCT 1680GATCCCCTAG AAAAAAATCC TATCCATTGG GGATACCAAG GGAATTGGGC ATTATCTTGG 1740CAAGAGGATA CTGCGACTAA ATCCAAAGCA GCGACTCTTA CCTGGACAAA AACAGGATAC 1800AATCCGAATC CTGAGCGTCG TGGAACCTTA GTTGCTAACA CGCTATGGGG ATCCTTTGTT 1860GATGTGCGCT CCATACAACA GCTTGTAGCC ACTAAAGTAC GCCAATCTCA AGAAACTCGC 1920GGCATCTGGT GTGAAGGGAT CTCGAACTTC TTCCATAAAG ATAGCACGAA GATAAATAAA 1980GGTTTTCGCC ACATAAGTGC AGGTTATGTT GTAGGAGCGA CTACAACATT AGCTTCTGAT 2040AATCTTATCA CTGCAGCCTT CTGCCAATTA TTCGGGAAAG ATAGAGATCA CTTTATAAAT 2100AAAAATAGAG CTTCTGCCTA TGCAGCTTCT CTCCATCTCC AGCATCTAGC GACCTTGTCT 2160TCTCCAAGCT TGTTACGCTA CCTTCCTGGA TCTGAAAGTG AGCAGCCTGT CCTCTTTGAT 2220GCTCAGATCA GCTATATCTA TAGTAAAAAT ACTATGAAAA CCTATTACAC CCAAGCACCA 2280AAGGGAGAGA GCTCGTGGTA TAATGACGGT TGCGCTCTGG AACTTGCGAG CTCCCTACCA 2340CACACTGCTT TAAGCCATGA GGGTCTCTTC CACGCGTATT TTCCTTTCAT CAAAGTAGAA 2400GCTTCGTACA TACACCAAGA TAGCTTCAAA GAACGTAATA CTACCTTGGT ACGATCTTTC 2460GATAGCGGTG ATTTAATTAA CGTCTCTGTG CCTATTGGAA TTACCTTCGA GAGATTCTCG 2520AGAAACGAGC GTGCGTCTTA CGAAGCTACT GTCATCTACG TTGCCGATGT CTATCGTAAG 2580AATCCTGACT GCACGACAGC TCTCCTAATC AACAATACCT CGTGGAAAAC TACAGGAACG 2640AATCTCTCAA GACAAGCTGG TATCGGAAGA GCAGGGATCT TTTATGCCTT CTCTCCAAAT 2700CTTGAGGTCA CAAGTAACCT ATCTATGGAA ATTCGTGGAT CTTCACGCAG CTACAATGCA 2760GATCTTGGAG GTAAGTTCCA GTTCTAA 2787(2)SEQ ID NO14的信息(i)序列特征(A)長(zhǎng)度928氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO14Met Lys Ser Ser Leu His Trp Phe Val Ile Ser Ser Ser Leu Ala Leu1 5 10 15Pro Leu Ser Leu Asn Phe Ser Ala Phe Ala Ala ValVal Glu Ile Asn20 25 30Leu Gly Pro Thr Asn Ser Phe Ser Gly Pro Gly Thr Tyr Thr Pro Pro35 40 45Ala Gln Thr Thr Asn Ala Asp Gly Thr Ile Tyr Asn Leu Thr Gly Asp50 55 60Val Ser Ile Thr Asn Ala Gly Ser Pro Thr Ala Leu Thr Ala Ser Cys65 70 75 80Phe Lys Glu Thr Thr Gly Asn Leu Ser Phe Gln Gly His Gly Tyr Gln85 90 95Phe Leu Leu Gln Asn Ile Asp Ala Gly Ala Asn Cys Thr Phe Thr Asn100 105 110Thr Ala Ala Asn Lys Leu Leu Ser Phe Ser Gly Phe Ser Tyr Leu Ser115 120 125Leu Ile Gln Thr Thr Asn Ala Thr Thr Gly Thr Gly Ala Ile Lys Ser130 135 140Thr Gly Ala Cys Ser Ile Gln Ser Asn Tyr Ser Cys Tyr Phe Gly Gln145 150 155 160Asn Phe Ser Asn Asp Asn Gly Gly Ala Leu Gln Gly Ser Ser Ile Ser165 170 175Leu Ser Leu Asn Pro Asn Leu Thr Phe Ala Lys Asn Lys Ala Thr Gln180 185 190Lys Gly Gly Ala Leu Tyr Ser Thr Gly Gly lle Thr Ile Asn Asn Thr195 200 205Leu Asn Ser Ala Ser Phe Ser Glu Asn Thr Ala Ala Asn Asn Gly Gly210 215 220Ala Ile Tyr Thr Glu Ala Ser Ser Phe Ile Ser Ser Asn Lys Ala Ile225 230 235 240Ser Phe Ile Asn Asn Ser Val Thr Ala Thr Ser Ala Thr Gly Gly Ala
245250 255lle Tyr Cys Ser Ser Thr Ser Ala Pro Lys Pro Val Leu Thr Leu Ser260 265 270Asp Asn Gly Glu Leu Asn Phe Ile Gly Asn Thr Ala Ile Thr Ser Gly275 280 285Gly Ala Ile Tyr Thr Asp Asn Leu Val Leu Ser Ser Gly Gly Pro Thr290 295 300Leu Phe Lys Asn Asn Ser Ala Ile Asp Thr Ala Ala Pro Leu Gly Gly305 310 315 320Ala Ile Ala Ile Ala Asp Ser Gly Ser Leu Ser Leu Ser Ala Leu Gly325 330 335Gly Asp Ile Thr Phe Glu Gly Asn Thr Val Val Lys Gly Ala Ser Ser340 345 350Ser Gln Thr Thr Thr Arg Asn Ser Ile Asn Ile Gly Asn Thr Asn Ala355 360 365Lys Ile Val Gln Leu Arg Ala Ser Gln Gly Asn Thr Ile Tyr Phe Tyr370 375 380Asp Pro Ile Thr Thr Asn His Thr Ala Ala Leu Ser Asp Ala Leu Asn385 390 395 400Leu Asn Gly Pro Asp Leu Ala Gly Asn Pro Ala Tyr Gln Gly Thr Ile405 410 415Val Phe Ser Gly Glu Lys Leu Ser Glu Ala Glu Ala Ala Glu Ala Asp420 425 430Asn Leu Lys Ser Thr Ile Gln Gln Pro Leu Thr Leu Ala Gly Gly Gln435 440 445Leu Ser Leu Lys Ser Gly Val Thr Leu Val Ala Lys Ser Phe Ser Gln450 455 460Ser Pro Gly Ser Thr Leu Leu Met Asp Ala Gly Thr Thr Leu Glu Thr465 470 475 480Ala Asp Gly Ile Thr Ile Asn Asn Leu Val Leu Asn Val Asp Ser Leu485 490 495Lys Glu Thr Lys Lys Ala Thr Leu Lys Ala Thr Gln Ala Ser Gln Thr500 505 510Val Thr Leu Ser Gly Ser Leu Ser Leu Val Asp Pro Ser Gly Asn Val515 520 525Tyr Glu Asp Val Ser Trp Asn Asn Pro Gln Val Phe Ser Cys Leu Thr530 535 540Leu Thr Ala Asp Asp Pro Ala Asn Ile His Ile Thr Asp Leu Ala Ala545 550 555 560Asp Pro Leu Glu Lys Asn Pro Ile His Trp Gly Tyr Gln Gly Asn Trp565 570 575Ala Leu Ser Trp Gln Glu Asp Thr Ala Thr Lys Ser Lys Ala Ala Thr580585 590Leu Thr Trp Thr Lys Thr Gly Tyr Asn Pro Asn Pro Glu Arg Arg Gly595 600 605Thr Leu Val Ala Asn Thr Leu Trp Gly Ser Phe Val Asp Val Arg Ser610 615 620Ile Gln Gln Leu Val Ala Thr Lys Val Arg Gln Ser Gln Glu Thr Arg625 630 635 640Gly Ile Trp Cys Glu Gly Ile Ser Asn Phe Phe His Lys Asp Ser Thr645 650 655Lys Ile Asn Lys Gly Phe Arg His Ile Ser Ala Gly Tyr Val Val Gly
660 665670Ala Thr Thr Thr Leu Ala Ser Asp Asn Leu Ile Thr Ala Ala Phe Cys675 680 685Gln Leu Phe Gly Lys Asp Arg Asp His Phe Ile Asn Lys Asn Arg Ala690 695 700Ser Ala Tyr Ala Ala Ser Leu His Leu Gln His Leu Ala Thr Leu Ser705 710 715 720Ser Pro Ser Leu Leu Arg Tyr Leu Pro Gly Ser Glu Ser Glu Gln Pro725 730 735Val Leu Phe Asp Ala Gln Ile Ser Tyr Ile Tyr Ser Lys Asn Thr Met740 745 750Lys Thr Tyr Tyr Thr Gln Ala Pro Lys Gly Glu Ser Ser Trp Tyr Asn755 760 765Asp Gly Cys Ala Leu Glu Leu Ala Ser Ser Leu Pro His Thr Ala Leu770 775 780Ser His Glu Gly Leu Phe His Ala Tyr Phe Pro Phe Ile Lys Val Glu785 790 795 800Ala Ser Tyr Ile His Gln Asp Ser Phe Lys Glu Arg Asn Thr Thr Leu805 810 815Val Arg Ser Phe Asp Ser Gly Asp Leu Ile Asn Val Ser Val Pro Ile820 825 830Gly Ile Thr Phe Glu Arg Phe Ser Arg Asn Glu Arg Ala Ser Tyr Glu835 840 845Ala Thr Val Ile Tyr Val Ala Asp Val Tyr Arg Lys Asn Pro Asp Cys850 855 860Thr Thr Ala Leu Leu Ile Asn Asn Thr Ser Trp Lys Thr Thr Gly Thr865 870 875 880Asn Leu Ser Arg Gln Ala Gly Ile Gly Arg Ala Gly Ile Phe Tyr Ala885 890 895Phe Ser Pro Asn Leu Glu Val Thr Ser Asn Leu Ser Met Glu Ile Arg900 905 910Gly Ser Ser Arg Ser Tyr Asn Ala Asp Leu Gly Gly Lys Phe Gln Phe915 920 925(2)SEQ ID NO15的信息(i)序列特征(A)長(zhǎng)度2793堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO15ATGAAAATAC CCTTGCACAA ACTCCTGATC TCTTCGACTC TTGTCACTCC CATTCTATTG 60AGCATTGCAA CTTACGGAGC AGATGCTTCT TTATCCCCTA CAGATAGCTT TGATGGAGCG 120GGCGGCTCTA CATTTACTCC AAAATCTACA GCAGATGCCA ATGGAACGAA CTATGTCTTA 180TCAGGAAATG TCTATATAAA CGATGCTGGG AAAGGCACAG CATTAACAGG CTGCTGCTTT 240ACAGAAACTA CGGGTGATCT GACATTTACT GGAAAGGGAT ACTCATTTTC ATTCAACACG 300GTAGATGCGG GTTCGAATGC AGGAGCTGCG GCAAGCACAA CTGCTGATAA AGCCCTAACA 360TTCACAGGAT TTTCTAACCT TTCCTTCATT GCAGCTCCTG GAACTACAGT TGCTTCAGGA 420AAAAGTACTT TAAGTTCTGC AGGAGCCTTA AATCTTACCG ATAATGGAAC GATTCTCTTT 480AGCCAAAACG TCTCCAATGA AGCTAATAAC AATGGCGGAG CGATCACCAC AAAAACTCTT 540TCTATTTCTG GGAATACCTC TTCTATAACC TTCACTAGTA ATAGCGCAAA AAAATTAGGT 600GGAGCGATCT ATAGCTCTGC GGCTGCAAGT ATTTCAGGAA ACACCGGCCA GTTAGTCTTT 660ATGAATAATA AAGGAGAAAC TGGGGGCGGG GCTCTGGGCT TTGAAGCCAG CTCCTCGATT 720ACTCAAAATA GCTCCCTTTT CTTCTCTGGA AACACTGCAA CAGATGCTGC AGGCAAGGGC 780GGGGCCATTT ATTGTGAAAA AACAGGAGAG ACTCCTACTC TTACTATCTC TGGAAATAAA 840AGTCTGACCT TCGCCGAGAA CTCTTCAGTA ACTCAAGGCG GAGCAATCTG TGCCCATGGT 900CTAGATCTTT CCGCTGCTGG CCCTACCCTA TTTTCAAATA ATAGATGCGG GAACACAGCT 960GCAGGCAAGG GCGGCGCTAT TGCAATTGCC GACTCTGGAT CTTTAAGTCT CTCTGCAAAT 1020CAAGGAGACA TCACGTTCCT TGGCAACACT CTAACCTCAA CCTCCGCGCC AACATCGACA 1080CGGAATGCTA TCTACCTGGG ATCGTCAGCA AAAATTACGA ACTTAAGGGC AGCCCAAGGC 1140CAATCTATCT ATTTCTATGA TCCGATTGCA TCTAACACCA CAGGAGCTTC AGACGTTCTG 1200ACCATCAACC AACCGGATAG CAACTCGCCT TTAGATTATT CAGGAACGAT TGTATTTTCT 1260GGGGAAAAGC TCTCTGCAGA TGAAGCGAAA GCTGCTGATA ACTTCACATC TATATTAAAG 1320CAACCATTGG CTCTAGCCTC TGGAACCTTA GCACTCAAAG GAAATGTCGA GTTAGATGTC 1380AATGGTTTCA CACAGACTGA AGGCTCTACA CTCCTCATGC AACCAGGAAC AAAGCTCAAA 1440GCAGATACTG AAGCTATCAG TCTTACCAAA CTTGTCGTTG ATCTTTCTGC CTTAGAGGGA 1500AATAAGAGTG TGTCCATTGA AACAGCAGGA GCCAACAAAA CTATAACTCT AACCTCTCCT 1560CTTGTTTTCC AAGATAGTAG CGGCAATTTT TATGAAAGCC ATACGATAAA CCAAGCCTTC 1620ACGCAGCCTT TGGTGGTATT CACTGCTGCT ACTGCTGCTA GCGATATTTA TATCGATGCG 1680CTTCTCACTT CTCCAGTACA AACTCCAGAA CCTCATTACG GGTATCAGGG ACATTGGGAA 1740GCCACTTGGG CAGACACATC AACTGCAAAA TCAGGAACTA TGACTTGGGT AACTACGGGC 1800TACAACCCTA ATCCTGAGCG TAGAGCTTCC GTAGTTCCCG ATTCATTATG GGCATCCTTT 1860ACTGACATTC GCACTCTACA GCAGATCATG ACATCTCAAG CGAATAGTAT CTATCAGCAA 1920CGAGGACTCT GGGCATCAGG AACTGCGAAT TTCTTCCATA AGGATAAATC AGGAACTAAC 1980CAAGCATTCC GACATAAAAG CTACGGCTAT ATTGTTGGAG GAAGTGCTGA AGATTTTTCT 2040GAAAATATCT TCAGTGTAGC TTTCTGCCAG CTCTTCGGTA AAGATAAAGA CCTGTTTATA 2100GTTGAAAATA CCTCTCATAA CTATTTAGCG TCGCTATACC TGCAACATCG AGCATTCCTA 2160GGAGGACTTC CCATGCCCTC ATTTGGAAGT ATCACCGACA TGCTGAAAGA TATTCCTCTC 2220ATTTTGAATG CCCAGCTAAG CTACAGCTAC ACTAAAAATG ATATGGATAC TCGCTATACT 2280TCCTATCCTG AAGCTCAAGG TTCTTGGACC AATAATTCTG GGGCTCTAGA GCTCGGAGGA 2340TCTCTGGCTC TATATCTCCC TAAAGAAGCA CCGTTCTTCC AGGGATATTT CCCCTTCTTA 2400AAGTTCCAGG CAGTCTACAG CCGCCAACAA AACTTTAAAG AGAGTGGCGC TGAAGCCCGT 2460GCTTTTGATG ATGGAGACCT AGTGAACTGC TCTATCCCTG TCGGCATTCG GTTAGAAAAA 2520ATCTCCGAAG ATGAAAAAAA TAATTTCGAG ATTTCTCTAG CCAACATTGG TGATGTGTAT 2580CGTAAAAATC CCCGTTCGCG TACTTCTCTA ATGGTCAGTG GAGCCTCTTG GACTTCGCTA 2640TGTAAAAACC TCGCACGACA AGCCTTCTTA GCAAGTGCTG GAAGCCATCT GACTCTCTCC 2700CCTCATGTAG AACTCTCTGG GGAAGCTGCT TATGAGCTTC GTGGCTCAGC ACACATCTAC 2760AATGTAGATT GTGGGCTAAG ATACTCATTC TAG 2793(2)SEQ ID NO16的信息(i)序列特征(A)長(zhǎng)度930氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽
(xi)序列描述SEQ ID NO16Met Lys Ile Pro Leu His Lys Leu Leu Ile Ser Ser Thr Leu Val Thr1 5 10 15Pro Ile Leu Leu Ser Ile Ala Thr Tyr Gly Ala Asp Ala Ser Leu Ser20 25 30Pro Thr Asp Ser Phe Asp Gly Ala Gly Gly Ser Thr Phe Thr Pro Lys35 40 45Ser Thr Ala Asp Ala Asn Gly Thr Asn Tyr Val Leu Ser Gly Asn Val50 55 60Tyr Ile Asn Asp Ala Gly Lys Gly Thr Ala Leu Thr Gly Cys Cys Phe65 70 75 80Thr Glu Thr Thr Gly Asp Leu Thr Phe Thr Gly Lys Gly Tyr Ser Phe85 90 95Ser Phe Asn Thr Val Asp Ala Gly Ser Asn Ala Gly Ala Ala Ala Ser100 105 110Thr Thr Ala Asp Lys Ala Leu Thr Phe Thr Gly Phe Ser Asn Leu Ser115 120 125Phe Ile Ala Ala Pro Gly Thr Thr Val Ala Ser Gly Lys Ser Thr Leu130 135 140Ser Ser Ala Gly Ala Leu Asn Leu Thr Asp Asn Gly Thr Ile Leu Phe145 150 155 160Ser Gln Asn Val Ser Asn Glu Ala Asn Asn Asn Gly Gly Ala Ile Thr165 170 175Thr Lys Thr Leu Ser Ile Ser Gly Asn Thr Ser Ser Ile Thr Phe Thr180 185 190Ser Asn Ser Ala Lys Lys Leu Gly Gly Ala Ile Tyr Ser Ser Ala Ala195 200 205Ala Ser Ile Ser Gly Asn Thr Gly Gln Leu Val Phe Met Asn Asn Lys210 215 220Gly Glu Thr Gly Gly Gly Ala Leu Gly Phe Glu Ala Ser Ser Ser Ile225 230 235 240Thr Gln Asn Ser Ser Leu Phe Phe Ser Gly Asn Thr Ala Thr Asp Ala245 250 255Ala Gly Lys Gly Gly Ala Ile Tyr Cys Glu Lys Thr Gly Glu Thr Pro260 265 270Thr Leu Thr Ile Ser Gly Asn Lys Ser Leu Thr Phe Ala Glu Asn Ser275 280 285Ser Val Thr Gln Gly Gly Ala Ile Cys Ala His Gly Leu Asp Leu Ser290 295 300Ala Ala Gly Pro Thr Leu Phe Ser Asn Asn Arg Cys Gly Asn Thr Ala305 310 315 320Ala Gly Lys Gly Gly Ala Ile Ala Ile Ala Asp Ser Gly Ser Leu Ser325 330 335Leu Ser Ala Asn Gln Gly Asp Ile Thr Phe Leu Gly Asn Thr Leu Thr340 345 350Ser Thr Ser Ala Pro Thr Ser Thr Arg Asn Ala Ile Tyr Leu Gly Ser355 360 365Ser Ala Lys Ile Thr Asn Leu Arg Ala Ala Gln Gly Gln Ser Ile Tyr370 375 380Phe Tyr Asp Pro Ile Ala Ser Asn Thr Thr Gly Ala Ser Asp Val Leu385 390 395 400Thr Ile Asn Gln Pro Asp Ser Asn Ser Pro Leu Asp Tyr Ser Gly Thr405 410 415Ile Val Phe Ser Gly Glu Lys Leu Ser Ala Asp Glu Ala Lys Ala Ala420 425 430Asp Asn Phe Thr Ser Ile Leu Lys Gln Pro Leu Ala Leu Ala Ser Gly435 440 445Thr Leu Ala Leu Lys Gly Asn Val Glu Leu Asp Val Asn Gly Phe Thr450 455 460Gln Thr Glu Gly Ser Thr Leu Leu Met Gln Pro Gly Thr Lys Leu Lys465 470 475 480Ala Asp Thr Glu Ala Ile Ser Leu Thr Lys Leu Val Val Asp Leu Ser485 490 495Ala Leu Glu Gly Asn Lys Ser Val Ser Ile Glu Thr Ala Gly Ala Asn500 505 510Lys Thr Ile Thr Leu Thr Ser Pro Leu Val Phe Gln Asp Ser Ser Gly515 520 525Asn Phe Tyr Glu Ser His Thr Ile Asn Gln Ala Phe Thr Gln Pro Leu530 535 540Val Val Phe Thr Ala Ala Thr Ala Ala Ser Asp Ile Tyr Ile Asp Ala545 550 555 560Leu Leu Thr Ser Pro Val Gln Thr Pro Glu Pro His Tyr Gly Tyr Gln565 570 575Gly His Trp Glu Ala Thr Trp Ala Asp Thr Ser Thr Ala Lys Ser Gly580 585 590Thr Met Thr Trp Val Thr Thr Gly Tyr Asn Pro Asn Pro Glu Arg Arg595 600 605Ala Ser Val Val Pro Asp Ser Leu Trp Ala Ser Phe Thr Asp Ile Arg610 615 620Thr Leu Gln Gln Ile Met Thr Ser Gln Ala Asn Ser Ile Tyr Gln Gln625 630 635 640Arg Gly Leu Trp Ala Ser Gly Thr Ala Asn Phe Phe His Lys Asp Lys645 650 655Ser Gly Thr Asn Gln Ala Phe Arg His Lys Ser Tyr Gly Tyr Ile Val660 665 670Gly Gly Ser Ala Glu Asp Phe Ser Glu Asn Ile Phe Ser Val Ala Phe675 680 685Cys Gln Leu Phe Gly Lys Asp Lys Asp Leu Phe Ile Val Glu Asn Thr690 695 700Ser His Asn Tyr Leu Ala Ser Leu Tyr Leu Gln His Arg Ala Phe Leu705 710 715 720Gly Gly Leu Pro Met Pro Ser Phe Gly Ser Ile Thr Asp Met Leu Lys725 730 735Asp Ile Pro Leu Ile Leu Asn Ala Gln Leu Ser Tyr Ser Tyr Thr Lys740 745 750Asn Asp Met Asp Thr Arg Tyr Thr Ser Tyr Pro Glu Ala Gln Gly Ser755 760765Trp Thr Asn Asn Ser Gly Ala Leu Glu Leu Gly Gly Ser Leu Ala Leu770 775 780Tyr Leu Pro Lys Glu Ala Pro Phe Phe Gln Gly Tyr Phe Pro Phe Leu785 790 795 800Lys Phe Gln Ala Val Tyr Ser Arg Gln Gln Asn Phe Lys Glu Ser Gly805 810 815Ala Glu Ala Arg Ala Phe Asp Asp Gly Asp Leu Val Asn Cys Ser Ile820 825 830Pro Val Gly Ile Arg Leu Glu Lys Ile Ser Glu Asp Glu Lys Asn Asn835 840 845Phe Glu Ile Ser Leu Ala Asn Ile Gly Asp Val Tyr Arg Lys Asn Pro850 855 860Arg Ser Arg Thr Ser Leu Met Val Ser Gly Ala Ser Trp Thr Ser Leu865 870 875 880Cys Lys Asn Leu Ala Arg Gln Ala Phe Leu Ala Ser Ala Gly Ser His885 890 895Leu Thr Leu Ser Pro His Val Glu Leu Ser Gly Glu Ala Ala Tyr Glu900 905 910Leu Arg Gly Ser Ala His Ile Tyr Asn Val Asp Cys Gly Leu Arg Tyr915 920 925Ser Phe930(2)SEQ ID NO17的信息(i)序列特征(A)長(zhǎng)度840堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO17GAAGACAATA TAAGGTACCG TCATAACAGC GGGGGTTATG CACTAGGGAT CACAGCAACA 60ACTCCTGCCG AGGATCAGCT TACTTTTGCC TTCTGCCAGC TCTTTGCTAG AGATCGCAAT 120CATATTACAG GTAAGAACCA CGGAGATACT TACGGTGCCT CTTTGTATTT CCACCATACA 180GAAGGGCTCT TCGACATCGC CAATTTCCTC TGGGGAAAAG CAACCCGAGC TCCCTGGGTG 240CTCTCTGAGA TCTCCCAGAT CATTCCTTTA TCGTTCGATG CTAAATTCAG TTATCTCCAT 300ACAGACAACC ACATGAAGAC ATATTATACC GATAACTCTA TCATCAAGGG TTCTTGGAGA 360AACGATGCCT TCTGTGCAGA TCTTGGAGCT AGCCTGCCTT TTGTTATTTC CGTTCCGTAT 420CTTCTGAAAG AAGTCGAACC TTTTGTCAAA GTACAGTATA TCTATGCGCA TCAGCAAGAC 480TTCTACGAGC GTCATGCTGA AGGACGCGCT TTCAATAAAA GCGAGCTTAT CAACGTAGAG 540ATTCCTATAG GCGTCACCTT CGAAAGAGAC TCAAAATCAG AAAAGGGAAC TTACGATCTT 600ACTCTTATGT ATATACTCGA TGCTTACCGA CGCAATCCTA AATGTCAAAC TTCCCTAATA 660GCTAGCGATG CTAACTGGAT GGCCTATGGT ACCAACCTCG CACGACAAGG TTTTTCTGTT 720CGTGCTGCGA ACCATTTCCA AGTGAACCCC CACATGGAAA TCTTCGGTCA ATTCGCTTTT 780GAAGTACGAA GTTCTTCACG AAATTATAAT ACAAACCTAG GCTCTAAGTT TTGTTTCTAG 840(2)SEQ ID NO18的信息(i)序列特征(A)長(zhǎng)度279氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性
(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO18Glu Asp Asn Ile Arg Tyr Arg His Asn Ser Gly Gly Tyr Ala Leu Gly1 5 10 15Ile Thr Ala Thr Thr Pro Ala Glu Asp Gln Leu Thr Phe Ala Phe Cys20 25 30Gln Leu Phe Ala Arg Asp Arg Asn His Ile Thr Gly Lys Asn His Gly35 40 45Asp Thr Tyr Gly Ala Ser Leu Tyr Phe His His Thr Glu Gly Leu Phe50 55 60Asp Ile Ala Asn Phe Leu Trp Gly Lys Ala Thr Arg Ala Pro Trp Val65 70 75 80Leu Ser Glu Ile Ser Gln Ile Ile Pro Leu Ser Phe Asp Ala Lys Phe85 90 95Ser Tyr Leu His Thr Asp Asn His Met Lys Thr Tyr Tyr Thr Asp Asn100 105 110Ser Ile Ile Lys Gly Ser Trp Arg Asn Asp Ala Phe Cys Ala Asp Leu115 120 125Gly Ala Ser Leu Pro Phe Val Ile Ser Val Pro Tyr Leu Leu Lys Glu130 135 140Val Glu Pro Phe Val Lys Val Gln Tyr Ile Tyr Ala His Gln Gln Asp145 150 155 160Phe Tyr Glu Arg His Ala Glu Gly Arg Ala Phe Asn Lys Ser Glu Leu165 170 175lle Asn Val Glu Ile Pro Ile Gly Val Thr Phe Glu Arg Asp Ser Lys180 185 190Ser Glu Lys Gly Thr Tyr Asp Leu Thr Leu Met Tyr Ile Leu Asp Ala195 200 205Tyr Arg Arg Asn Pro Lys Cys Gln Thr Ser Leu Ile Ala Ser Asp Ala210 215 220Asn Trp Met Ala Tyr Gly Thr Asn Leu Ala Arg Gln Gly Phe Ser Val225 230 235 240Arg Ala Ala Asn His Phe Gln Val Asn Pro His Met Glu Ile Phe Gly245 250 255Gln Phe Ala Phe Glu Val Arg Ser Ser Ser Arg Asn Tyr Asn Thr Asn260 265 270Leu Gly Ser Lys Phe Cys Phe275(2)SEQ ID NO19的信息(i)序列特征(A)長(zhǎng)度1545堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO19ATGACCATAC TTCGAAATTT TCTTACCTGC TCGGCTTTAT TCCTCGCTCT CCCTGCAGCA 60GCACAAGTTG TATATCTTCA TGAAAGTGAT GGTTATAACG GTGCTATCAA TAATAAAAGC 120TTAGAACCTA AAATTACCTG TTATCCAGAA GGAACTTCTT ACATCTTTCT AGATGACGTG 180AGGATTTCCA ACGTTAAGCA TGATCAAGAA GATGCTGGGG TTTTTATAAA TCGATCTGGG 240AATCTTTTTT TCATGGGCAA CCGTTGCAAC TTCACTTTTC ACAACCTTAT GACCGAGGGT 300TTTGGCGCTG CCATTTCGAA CCGCGTTGGA GACACCACTC TCACTCTCTC TAATTTTTCT 360TACTTAACGT TCACCTCAGC ACCTCTACTA CCTCAAGGAC AAGGAGCGAT TTATAGTCTT 420GGTTCCGTGA TGATCGAAAA TAGTGAGGAA GTGACTTTCT GTGGGAACTA CTCTTCGTGG 480AGTGGAGCTG CGATTTATAC TCCCTACCTT TTAGGTTCTA AGGCGAGTCG TCCTTCAGTA 540AATCTCAGCG GGAACCGCTA CCTGGTGTTT AGAGACTATG TGAGCCAAGG TTATGGCGGC 600GCCGTATCTA CCCACAATCT CACACTCACG ACTCGAGGAC CTTCGTGTTT TGAAAATAAT 660CATGCTTATC ATGACGTGAA TAGTAATGGA GGAGCCATTG CCATTGCTCC TGGAGGATCG 720ATCTCTATAT CCGTGAAAAG CGGAGATCTC ATCTTCAAAG GAAATACAGC ATCACAAGAC 780GGAAATACAA TACACAACTC CATCCATCTG CAATCTGGAG CACAGTTTAA GAACCTACGT 840GCTGTTTCAG AATCCGGAGT TTATTTCTAT GATCCTATAA GCCATAGCGA GTCGCATAAA 900ATTACAGATC TTGTAATCAA TGCTCCTGAA GGAAAGGAAA CTTATGAAGG AACAATTAGC 960TTCTCAGGAC TATGCCTGGA TGATCATGAA GTTTGTGCGG AAAATCTTAC TTCCACAATC 1020CTACAAGATG TCACATTAGC AGGAGGAACT CTCTCTCTAT CGGATGGGGT TACCTTGCAA 1080CTGCATTCTT TTAAGCAGGA AGCAAGCTCT ACGCTTACTA TGTCTCCAGG AACCACTCTG 1140CTCTGCTCAG GAGATGCTCG GGTTCAGAAT CTGCACATCC TGATTGAAGA TACCGACAAC 1200TTTGTTCCTG TAAGGATTCG CGCCGAGGAC AAGGATGCTC TTGTCTCATT AGAAAAACTT 1260AAAGTTGCCT TTGAGGCTTA TTGGTCCGTC TATGACTTTC CTCAATTTAA GGAAGCCTTT 1320ACGATTCCTC TTCTTGAACT TCTAGGGCCT TCTTTTGACA GTCTTCTCCT AGGGGAGACC 1380ACTTTGGAGA GAACCCAAGT CACAACAGAG AATGACGCCG TTCGAGGTTT CTGGTCCCTA 1440AGCTGGGAAG AGTACCCCCC TTCTCTGGAT AAAGACAGAA GGATCACACC AACTAAGAAA 1500ACTGTTTTCC TCACTTGGAA TCCTGAGATC ACTTCTACGC CATAA 1545(2)SEQ ID NO20的信息(i)序列特征(A)長(zhǎng)度514氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO20Met Thr Ile Leu Arg Asn Phe Leu Thr Cys Ser Ala Leu Phe Leu Ala1 5 10 15Leu Pro Ala Ala Ala Gln Val Val Tyr Leu His Glu Ser Asp Gly Tyr20 25 30Asn Gly Ala Ile Asn Asn Lys Ser Leu Glu Pro Lys Ile Thr Cys Tyr35 40 45Pro Glu Gly Thr Ser Tyr Ile Phe Leu Asp Asp Val Arg Ile Ser Asn50 55 60Val Lys His Asp Gln Glu Asp Ala Gly Val Phe Ile Asn Arg Ser Gly65 70 75 80Asn Leu Phe Phe Met Gly Asn Arg Cys Asn Phe Thr Phe His Asn Leu85 90 95Met Thr Glu Gly Phe Gly Ala Ala Ile Ser Asn Arg Val Gly Asp Thr100 105 110Thr Leu Thr Leu Ser Asn Phe Ser Tyr Leu Thr Phe Thr Ser Ala Pro115 120 125Leu Leu Pro Gln Gly Gln Gly Ala Ile Tyr Ser Leu Gly Ser Val Met130 135 140Ile Glu Asn Ser Glu Glu Val Thr Phe Cys Gly Asn Tyr Ser Ser Trp145 150 155 160Ser Gly Ala Ala Ile Tyr Thr Pro Tyr Leu Leu Gly Ser Lys Ala Ser165 170 175Arg Pro Ser Val Asn Leu Ser Gly Asn Arg Tyr Leu Val Phe Arg Asp180 185 190Tyr Val Ser Gln Gly Tyr Gly Gly Ala Val Ser Thr His Asn Leu Thr195 200 205Leu Thr Thr Arg Gly Pro Ser Cys Phe Glu Asn Asn His Ala Tyr His210 215 220Asp Val Asn Ser Asn Gly Gly Ala Ile Ala Ile Ala Pro Gly Gly Ser225 230 235 240Ile Ser Ile Ser Val Lys Ser Gly Asp Leu Ile Phe Lys Gly Asn Thr245 250 255Ala Ser Gln Asp Gly Asn Thr Ile His Asn Ser Ile His Leu Gln Ser260 265 270Gly Ala Gln Phe Lys Asn Leu Arg Ala Val Ser Glu Ser Gly Val Tyr275 280 285Phe Tyr Asp Pro Ile Ser His Ser Glu Ser His Lys Ile Thr Asp Leu290 295 300Val Ile Asn Ala Pro Glu Gly Lys Glu Thr Tyr Glu Gly Thr Ile Ser305 310 315 320Phe Ser Gly Leu Cys Leu Asp Asp His Glu Val Cys Ala Glu Asn Leu325 330 335Thr Ser Thr Ile Leu Gln Asp Val Thr Leu Ala Gly Gly Thr Leu Ser340 345 350Leu Ser Asp Gly Val Thr Leu Gln Leu His Ser Phe Lys Gln Glu Ala355 360 365Ser Ser Thr Leu Thr Met Ser Pro Gly Thr Thr Leu Leu Cys Ser Gly370 375 380Asp Ala Arg Val Gln Asn Leu His Ile Leu Ile Glu Asp Thr Asp Asn385 390 395 400Phe Val Pro Val Arg Ile Arg Ala Glu Asp Lys Asp Ala Leu Val Ser405 410 415Leu Glu Lys Leu Lys Val Ala Phe Glu Ala Tyr Trp Ser Val Tyr Asp420 425 430Phe Pro Gln Phe Lys Glu Ala Phe Thr Ile Pro Leu Leu Glu Leu Leu435 440 445Gly Pro Ser Phe Asp Ser Leu Leu Leu Gly Glu Thr Thr Leu Glu Arg450 455 460Thr Gln Val Thr Thr Glu Asn Asp Ala Val Arg Gly Phe Trp Ser Leu465 470 475 480Ser Trp Glu Glu Tyr Pro Pro Ser Leu Asp Lys Asp Arg Arg Ile Thr485 490 495Pro Thr Lys Lys Thr Val Phe Leu Thr Trp Asn Pro Glu Ile Thr Ser500 505 510Thr Pro(2)SEQ ID NO21的信息(i)序列特征(A)長(zhǎng)度787堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO21ATGAAAACGT CTATTCGTAA GTTCTTAATT TCTACCACAC TGGCGCCATG TTTTGCTTCA 60ACAGCGTTTA CTGTAGAAGT TATCATGCCT TCCGAGAACT TTGATGGATC GAGTGGGAAG 120ATTTTTCCTT ACACAACACT TTCTGATCCT AGAGGGACAC TCTGTATTTT TTCAGGGGAT 180CTCTACATTG CGAATCTTGA TAATGCCATA TCCAGAACCT CTTCCAGTTG CTTTAGCAAT 240AGGGCGGGAG CACTACAAAT CTTAGGAAAA GGTGGGGTTT TCTCCTTCTT AAATATCCGT 300TCTTCAGCTG ACGGAGCCGC GATTAGTAGT GTAATCACCC AAAATCCTGA ACTATGTCCC 360TTGAGTTTTT CAGGATTTAG TCAGATGATC TTCGATAACT GTGAATCTTT GACTTCAGAT 420ACCTCAGCGA GTAATGTCAT ACCTCACGCA TCGGCGATTT ACGCTACAAC GCCCATGCTC 480TTTACAAACA ATGACTCCAT ACTATTCCAA TACAACCGTT CTGCAGGATT TGGAGCTGCC 540ATTCGAGGCA CAAGCATCAC AATAGAAAAT ACGAAAAAGA GCCTTCTCTT TAATGGTAAT 600GGATCCATCT CTAATGGAGG GGCCCTCACG GGATCTGCAG CGATCAACCT CATCAACAAT 660AGCGCTCCTG TGATTTTCTC AACGAATGCT ACAGGGATCT ATGGTGGGGC TATTTACCTT 720ACCGGAGGAT CTATGCTCAC CTCTGGGAAC CTCTCAGGAG TCTTGTTCGT TTATAATAGC 780TCGCGCT 787(2)SEQ ID NO22的信息(i)序列特征(A)長(zhǎng)度262氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO22Met Lys Thr Ser Ile Arg Lys Phe Leu Ile Ser Thr Thr Leu Ala Pro1 5 10 15Cys Phe Ala Ser Thr Ala Phe Thr Val Glu Val Ile Met Pro Ser Glu20 25 30Asn Phe Asp Gly Ser Ser Gly Lys Ile Phe Pro Tyr Thr Thr Leu Ser35 40 45Asp Pro Arg Gly Thr Leu Cys Ile Phe Ser Gly Asp Leu Tyr Ile Ala50 55 60Asn Leu Asp Asn Ala Ile Ser Arg Thr Ser Ser Ser Cys Phe Ser Asn65 70 75 80Arg Ala Gly Ala Leu Gln Ile Leu Gly Lys Gly Gly Val Phe Ser Phe85 90 95Leu Asn Ile Arg Ser Ser Ala Asp Gly Ala Ala Ile Ser Ser Val Ile100 105 110Thr Gln Asn Pro Glu Leu Cys Pro Leu Ser Phe Ser Gly Phe Ser Gln115 120 125Met Ile Phe Asp Asn Cys Glu Ser Leu Thr Ser Asp Thr Ser Ala Ser130 135 140Asn Val Ile Pro His Ala Ser Ala Ile Tyr Ala Thr Thr Pro Met Leu145 150 155 160Phe Thr Asn Asn Asp Ser Ile Leu Phe Gln Tyr Asn Arg Ser Ala Gly165 170 175Phe Gly Ala Ala Ile Arg Gly Thr Ser Ile Thr Ile Glu Asn Thr Lys180 185 190Lys Ser Leu Leu Phe Asn Gly Asn Gly Ser Ile Ser Asn Gly Gly Ala195 200 205Leu Thr Gly Ser Ala Ala Ile Asn Leu Ile Asn Asn Ser Ala Pro Val210 215 220Ile Phe Ser Thr Asn Ala Thr Gly Ile Tyr Gly Gly Ala Ile Tyr Leu225 230 235 240Thr Gly Gly Ser Met Leu Thr Ser Gly Asn Leu Ser Gly Val Leu Phe245 250 255Val Tyr Asn Ser Ser Arg260(2)SEQ ID NO23的信息(i)序列特征(A)長(zhǎng)度2838堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型基因組DNA(xi)序列描述SEQ ID NO23ATGAAGACTT CAGTTTCTAT GTTGTTGGCC CTGCTTTGCT CGGGGGCTAG CTCTATTGTA 60CTCCATGCCG CAACCACTCC ACTAAATCCT GAAGATGGGT TTATTGGGGA GGGCAATACA 120AATACTTTTT CTCCGAAATC TACAACGGAT GCTGCAGGAA CTACCTACTC TCTCACAGGA 180GAGGTTCTGT TTATAGATCC GGGGAAAGGT GGTTCAATTA CAGGAACTTG CTTTGTAGAA 240ACTGCTGGCG ATCTTACATT TTTAGGTAAT GGAAATACCC TAAAGTTCCT GTCGGTAGAT 300GCAGGTGCTA ATATCGCGGT TGCTCATGTA CAAGGAAGTA AGAATTTAAG CTTCACAGAT 360TTCCTTTCTC TGGTGATCAC AGAATCTCCA AAATCCGCTG TTAGTACAGG AAAAGGTAGC 420CTAGTCAGTT CAGGTGCAGT CCAACTGCAA GATATAAACA CTCTAGTTCT TACAAGCAAT 480GCCTCTGTCG AAGATGGTGG CGTGATTAAA GGAAACTCCT GCTTGATTCA GGGAATCAAA 540AATAGTGCGA TTTTTGGACA AAATACATCT TCGAAAAAAG GAGGGGCGAT CTCCACGACT 600CAAGGACTCA CCATAGAGAA TAACTTAGGG ACGCTAAAGT TCAATGAAAA CAAAGCAGTG 660ACCTCAGGAG GCGCCTTAGA TTTAGGAGCC GCGTCTACAT TCACTGCGAA CCATGAGTTG 720ATATTTTCAC AAAATAAGAC TTCTGGGAAT GCTGCAAATG GCGGAGCCAT AAATTGCTCA 780GGCGACCTAA CATTTACTGA TAACACTTCT TTGTTACTTC AAGAAAATAG CACAATGCAG 840GATGGTGGAG CTTTGTGTAG CACAGGAACC ATAAGCATTA CCGGTAGTGA TTCTATCAAT 900GTGATAGGAA ATACTTCAGG ACAAAAAGGA GGAGCGATTT CTGCAGCTTC TCTCAAGATT 960TTGGGAGGGC AGGGAGGCGC TCTCTTTTCT AATAACGTAG TGACTCATGC CACCCCTCTA 1020GGAGGTGCCA TTTTTATCAA CACAGGAGGA TCCTTGCAGC TCTTCACTCA AGGAGGGGAT 1080ATCGTATTCG AGGGGAATCA GGTCACTACA ACAGCTCCAA ATGCTACCAC TAAGAGAAAT 1140GTAATTCACC TCGAGAGCAC CGCGAAGTGG ACGGGACTTG CTGCAAGTCA AGGTAACGCT 1200ATCTATTTCT ATGATCCCAT TACCACCAAC GATACGGGAG CAAGCGATAA CTTACGTATC 1260AATGAGGTCA GTGCAAATCA AAAGCTCTCG GGATCTATAG TATTTTCTGG AGAGAGATTG 1320TCGACAGCAG AAGCTATAGC TGAAAATCTT ACTTCGAGGA TCAACCAGCC TGTCACTTTA 1380GTAGAGGGGA GCTTAGAACT TAAACAGGGA GTGACCTTGA TCACACAAGG ATTCTCGCAG 1440GAGCCAGAAT CCACGCTTCT TTTGGATTTG GGGACCTCAT TACAAGCTTC TACAGAAGAT 1500ATCGTCATCA CAAATTCATC TATAAATGCC GATACCATTT ACGGAAAGAA TCCAATCAAT 1560ATTGTAGCTT CAGCAGCGAA TAAGAACATT ACCCTAACAG GAACCTTAGC ACTTGTAAAT 1620GCAGATGGAG CTTTGTATGA GAACCATACC TTGCAAGACT CTCAAGATTA TAGCTTTGTA 1680AAGTTATCTC CAGGAGCGGG AGGGACTATA ATTACTCAAG ATGCTTCTCA GAAGCTTCTT 1740GAAGTAGCTC CTTCTAGACC ACATTATGGC TATCAAGGAC ATTGGAATGT GCAAGTCATC 1800CCAGGAACGG GAACTCAACC GAGCCAGGCA AATTTAGAAT GGGTGCGGAC AGGATACCTT 1860CCGAATCCCG AACGGCAAGG ATTTTTAGTT CCCAATAGCC TGTGGGGTTC TTTTGTTGAT 1920CAGCGTGCTA TCCAAGAAAT CATGGTAAAT AGTAGCCAAA TCTTATGTCA GGAACGGGGA 1980GTCTGGGGAG CTGGAATTGC TAATTTCCTA CATAGAGATA AAATTAATGA GCACGGCTAT 2040CGCCATAGCG GTGTCGGTTA TCTTGTGGGA GTTGGCACTC ATGCTTTTTC TGATGCTACG 2100ATAAATGCGG CTTTTTGCCA GCTCTTCAGT AGAGATAAAG ACTACGTAGT ATCCAAAAAT 2160CATGGAACTA GCTACTCAGG GGTCGTATTT CTTGAGGATA CCCTAGAGTT TAGAAGTCCA 2220CAGGGATTCT ATACTGATAG CTCCTCAGAA GCTTGCTGTA ACCAAGTCGT CACTATAGAT 2280ATGCAGTTGT CTTACAGCCA TAGAAATAAT GATATGAAAA CCAAATACAC GACATATCCA 2340GAAGCTCAGG GATCTTGGGC AAATGATGTT TTTGGTCTTG AGTTTGGAGC GACTACATAC 2400TACTACCCTA ACAGTACTTT TTTATTTGAT TACTACTCTC CGTTTCTCAG GCTGCAGTGC 2460ACCTATGCTC ACCAGGAAGA CTTCAAAGAG ACAGGAGGTG AGGTTCGTCA CTTTACTAGC 2520GGAGATCTTT TCAATTTAGC AGTTCCTATT GGCGTGAAGT TTGAGAGATT TTCAGACTGT 2580AAAAGGGGAT CTrATGAACT TACCCTTGCT TATGTTCCTG ATGTGATTCG CAAAGATCCC 2640AAGAGCACGG CAACATTGGC TAGTGGAGCT ACGTGGAGCA CCCACGGAAA CAATCTCTCC 2700AGACAAGGAT TACAACTGCG TTTAGGGAAC CACTGTCTCA TAAATCCTGG AATTGAGGTG 2760TTCAGTCACG GAGCTATTGA ATTGCGGGGA TCCTCTCGTA ATTATAACAT CAATCTCGGG 2820GGTAAATACC GATTTTAA 2838(2)SEQ ID NO24的信息(i)序列特征(A)長(zhǎng)度946氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型肽(xi)序列描述SEQ ID NO24Met Lys Thr Ser Val Ser Met Leu Leu Ala Leu Leu Cys Ser Gly Ala1 5 10 15Ser Ser Ile Val Leu His Ala Ala Thr Thr Pro Leu Asn Pro Glu Asp20 25 30Gly Phe Ile Gly Glu Gly Asn Thr Asn Thr Phe Ser Pro Lys Ser Thr35 40 45Thr Asp Ala Ala Gly Thr Thr Tyr Ser Leu Thr Gly Glu Val Leu Phe50 55 60Ile Asp Pro Gly Lys Gly Gly Ser Ile Thr Gly Thr Cys Phe Val Glu65 70 75 80Thr Ala Gly Asp Leu Thr Phe Leu Gly Asn Gly Asn Thr Leu Lys Phe85 90 95Leu Ser Val Asp Ala Gly Ala Asn Ile Ala Val Ala His Val Gln Gly100 105 110Ser Lys Asn Leu Ser Phe Thr Asp Phe Leu Ser Leu Val Ile Thr Glu115 120 125Ser Pro Lys Ser Ala Val Ser Thr Gly Lys Gly Ser Leu Val Ser Ser130 135 140Gly Ala Val Gln Leu Gln Asp Ile Asn Thr Leu Val Leu Thr Ser Asn145 150 155 160Ala Ser Val Glu Asp Gly Gly Val Ile Lys Gly Asn Ser Cys Leu Ile165 170 175Gln Gly Ile Lys Asn Ser Ala Ile Phe Gly Gln Asn Thr Ser Ser Lys180 185 190Lys Gly Gly Ala Ile Ser Thr Thr Gln Gly Leu Thr Ile Glu Asn Asn195 200 205Leu Gly Thr Leu Lys Phe Asn Glu Asn Lys Ala Val Thr Ser Gly Gly210 215 220Ala Leu Asp Leu Gly Ala Ala Ser Thr Phe Thr Ala Asn His Glu Leu225 230 235 240Ile Phe Ser Gln Asn Lys Thr Ser Gly Asn Ala Ala Asn Gly Gly Ala245 250 255Ile Asn Cys Ser Gly Asp Leu Thr Phe Thr Asp Asn Thr Ser Leu Leu260 265 270Leu Gln Glu Asn Ser Thr Met Gln Asp Gly Gly Ala Leu Cys Ser Thr275 280 285Gly Thr Ile Ser Ile Thr Gly Ser Asp Ser Ile Asn Val Ile Gly Asn290 295 300Thr Ser Gly Gln Lys Gly Gly Ala Ile Ser Ala Ala Ser Leu Lys Ile305 310 315 320Leu Gly Gly Gln Gly Gly Ala Leu Phe Ser Asn Asn Val Val Thr His325 330 335Ala Thr Pro Leu Gly Gly Ala Ile Phe Ile Asn Thr Gly Gly Ser Leu340 345 350Gln Leu Phe Thr Gln Gly Gly Asp Ile Val Phe Glu Gly Asn Gln Val355 360 365Thr Thr Thr Ala Pro Asn Ala Thr Thr Lys Arg Asn Val Ile His Leu370 375 380Glu Ser Thr Ala Lys Trp Thr Gly Leu Ala Ala Ser Gln Gly Asn Ala385 390 395 400Ile Tyr Phe Tyr Asp Pro Ile Thr Thr Asn Asp Thr Gly Ala Ser Asp405 410 415Asn Leu Arg Ile Asn Glu Val Ser Ala Asn Gln Lys Leu Ser Gly Ser420 425 430Ile Val Phe Ser Gly Glu Arg Leu Ser Thr Ala Glu Ala Ile Ala Glu435 440 445Asn Leu Thr Ser Arg Ile Asn Gln Pro Val Thr Leu Val Glu Gly Ser450 455 460Leu Glu Leu Lys Gln Gly Val Thr Leu Ile Thr Gln Gly Phe Ser Gln465 470 475 480Glu Pro Glu Ser Thr Leu Leu Leu Asp Leu Gly Thr Ser Leu Gln Ala485 490 495Ser Thr Glu Asp Ile Val Ile Thr Asn Ser Ser Ile Asn Ala Asp Thr500 505 510Ile Tyr Gly Lys Asn Pro Ile Asn Ile Val Ala Ser Ala Ala Asn Lys515 520 525Asn Ile Thr Leu Thr Gly Thr Leu Ala Leu Val Asn Ala Asp Gly Ala530 535 540Leu Tyr Glu Asn His Thr Leu Gln Asp Ser Gln Asp Tyr Ser Phe Val545 550 555 560Lys Leu Ser Pro Gly Ala Gly Gly Thr Ile Ile Thr Gln Asp Ala Ser565 570 575Gln Lys Leu Leu Glu Val Ala Pro Ser Arg Pro His Tyr Gly Tyr Gln580 585 590Gly His Trp Asn Val Gln Val Ile Pro Gly Thr Gly Thr Gln Pro Ser595 600 605Gln Ala Asn Leu Glu Trp Val Arg Thr Gly Tyr Leu Pro Asn Pro Glu610 615 620Arg Gln Gly Phe Leu Val Pro Asn Ser Leu Trp Gly Ser Phe Val Asp625 630 635 640Gln Arg Ala Ile Gln Glu Ile Met Val Asn Ser Ser Gln Ile Leu Cys645 650 655Gln Glu Arg Gly Val Trp Gly Ala Gly Ile Ala Asn Phe Leu His Arg660 665 670Asp Lys Ile Asn Glu His Gly Tyr Arg His Ser Gly Val Gly Tyr Leu675 680 685Val Gly Val Gly Thr His Ala Phe Ser Asp Ala Thr Ile Asn Ala Ala690 695 700Phe Cys Gln Leu Phe Ser Arg Asp Lys Asp Tyr Val Val Ser Lys Asn705 710 715 720His Gly Thr Ser Tyr Ser Gly Val Val Phe Leu Glu Asp Thr Leu Glu725 730 735Phe Arg Ser Pro Gln Gly Phe Tyr Thr Asp Ser Ser Ser Glu Ala Cys740 745 750Cys Asn Gln Val Val Thr Ile Asp Met Gln Leu Ser Tyr Ser His Arg755 760 765Asn Asn Asp Met Lys Thr Lys Tyr Thr Thr Tyr Pro Glu Ala Gln Gly770 775 780Ser Trp Ala Asn Asp Val Phe Gly Leu Glu Phe Gly Ala Thr Thr Tyr785 790 795 800Tyr Tyr Pro Asn Ser Thr Phe Leu Phe Asp Tyr Tyr Ser Pro Phe Leu805 810 815Arg Leu Gln Cys Thr Tyr Ala His Gln Glu Asp Phe Lys Glu Thr Gly820 825 830Gly Glu Val Arg His Phe Thr Ser Gly Asp Leu Phe Asn Leu Ala Val835 840 845Pro IIe Gly Val Lys Phe Glu Arg Phe Ser Asp Cys Lys Arg Gly Ser850 855 860Tyr Glu Leu Thr Leu Ala Tyr Val Pro Asp Val Ile Arg Lys Asp Pro865 870 875 880Lys Ser Thr Ala Thr Leu Ala Ser Gly Ala Thr Trp Ser Thr His Gly885 890 895Asn Asn Leu Ser Arg Gln Gly Leu Gln Leu Arg Leu Gly Asn His Cys900 905 910Leu Ile Asn Pro Gly Ile Glu Val Phe Ser His Gly Ala Ile Glu Leu915 920 925Arg Gly Ser Ser Arg Asn Tyr Asn Ile Asn Leu Gly Gly Lys Tyr Arg930 935 940Phe945(2)SEQ ID NO25的信息(i)序列特征(A)長(zhǎng)度3000堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ix)特征(A)名稱/關(guān)鍵字編碼序列(B)位置259...3000(D)其它信息(xi)序列描述SEQ ID NO25ATCAGGTGAT AAAAGTTCCT CGTTAGCTAG TGACTGTAGG TGACATGAGA AAGCTAACAC 60GGAGGAAACT AAAACCCAAG GAATCGAAGT CTTCATGGTA ATGCTTTTGT TTTTTAGAGA 120ACTATTCGCA TCAATATAGA AACAAAATAA GTAAATCAAG TTAAAGATGA CAAAACAGCT 180GTCAAGAATT TTTATCTTGA CTCTCTGAGT TTTCTATTTT ATATGACGCA AGTAAGAATT 240TAATAATAAA GTGGGTTT ATG AAA TCG CAA TTT TCC TGG TTA GTG CTC TCT 291Met Lys Ser Gln Phe Ser Trp Leu Val Leu Ser1 5 10TCG ACA TTG GCA TGT TTT ACT AGT TGT TCC ACT GTT TTT GCT GCA ACT 339Ser Thr Leu Ala Cys Phe Thr Ser Cys Ser Thr Val Phe Ala Ala Thr15 20 25GCT GAA AAT ATA GGC CCC TCT GAT AGC TTT GAC GGA AGT ACT AAC ACA 387Ala Glu Asn Ile Gly Pro Ser Asp Ser Phe Asp Gly Ser Thr Asn Thr30 35 40GGC ACC TAT ACT CCT AAA AAT ACG ACT ACT GGA ATA GAC TAT ACT CTG 435Gly Thr Tyr Thr Pro Lys Asn Thr Thr Thr Gly Ile Asp Tyr Thr Leu45 50 55ACA GGA GAT ATA ACT CTG CAA AAC CTT GGG GAT TCG GCA GCT TTA ACG 483Thr Gly Asp Ile Thr Leu Gln Asn Leu Gly Asp Ser Ala Ala Leu Thr60 65 70 75AAG GGT TGT TTT TCT GAC ACT ACG GAA TCT TTA AGC TTT GCC GGT AAG 531Lys Gly Cys Phe Ser Asp Thr Thr Glu Ser Leu Ser Phe Ala Gly Lys80 85 90GGG TAC TCA CTT TCT TTT TTA AAT ATT AAG TCT AGT GCT GAA GGC GCA 579Gly Tyr Ser Leu Ser Phe Leu Asn Ile Lys Ser Ser Ala Glu Gly Ala95 100 105GCA CTT TCT GTT ACA ACT GAT AAA AAT CTG TCG CTA ACA GGA TTT TCG 627Ala Leu Ser Val Thr Thr Asp Lys Asn Leu Ser Leu Thr Gly Phe Ser110 115 120AGT CTT ACT TTC TTA GCG GCC CCA TCA TCG GTA ATC ACA ACC CCC TCA 675Ser Leu Thr Phe Leu Ala Ala Pro Ser Ser Val Ile Thr Thr Pro Ser125 130 135GGA AAA GGT GCA GTT AAA TGT GGA GGG GAT CTT ACA TTT GAT AAC AAT 723Gly Lys Gly Ala Val Lys Cys Gly Gly Asp Leu Thr Phe Asp Asn Asn140 145 150 155GGA ACT ATT TTA TTT AAA CAA GAT TAC TGT GAG GAA AAT GGC GGA GCC 771Gly Thr Ile Leu Phe Lys Gln Asp Tyr Cys Glu Glu Asn Gly Gly Ala160 165 170ATT TCT ACC AAG AAT CTT TCT TTG AAA AAC AGC ACG GGA TCG ATT TCT 819Ile Ser Thr Lys Asn Leu Ser Leu Lys Asn Ser Thr Gly Ser Ile Ser175 180 185TTT GAA GGG AAT AAA TCG AGC GCA ACA GGG AAA AAA GGT GGG GCT ATT 867Phe Glu Gly Asn Lys Ser Ser Ala Thr Gly Lys Lys Gly Gly Ala Ile190 195 200TGT GCT ACT GGT ACT GTA GAT ATT ACA AAT AAT ACG GCT CCT ACC CTC 915Cys Ala Thr Gly Thr Val Asp Ile Thr Asn Asn Thr Ala Pro Thr Leu205 210 215TTC TCG AAC AAT ATT GCT GAA GCT GCA GGT GGA GCT ATA AAT AGC ACA 963Phe Ser Asn Asn Ile Ala Glu Ala Ala Gly Gly Ala Ile Asn Ser Thr220 225 230 235GGA AAC TGT ACA ATT ACA GGG AAT ACG TCT CTT GTA TTT TCT GAA AAT 1011Gly Asn Cys Thr Ile Thr Gly Asn Thr Ser Leu Val Phe Ser Glu Asn240 245 250AGT GTG ACA GCG ACC GCA GGA AAT GGA GGA GCT CTT TCT GGA GAT GCC 1059Ser Val Thr Ala Thr Ala Gly Asn Gly Gly Ala Leu Ser Gly Asp Ala255 260 265GAT GTT ACC ATA TCT GGG AAT CAG AGT GTA ACT TTC TCA GGA AAC CAA 1107Asp Val Thr Ile Ser Gly Asn Gln Ser Val Thr Phe Ser Gly Asn Gln270 275 280GCT GTA GCT AAT GGC GGA GCC ATT TAT GCT AAG AAG CTT ACA CTG GCT 1155Ala Val Ala Asn Gly Gly Ala Ile Tyr Ala Lys Lys Leu Thr Leu Ala285 290 295TCC GGG GGG GGG GGG GGT ATC TCC TTT TCT AAC AAT ATA GTC CAA GGT 1203Ser Gly Gly Gly Gly Gly Ile Ser Phe Ser Asn Asn Ile Val Gln Gly300 305 310 315ACC ACT GCA GGT AAT GGT GGA GCC ATT TCT ATA CTG GCA GCT GGA GAG 1251Thr Thr Ala Gly Asn Gly Gly Ala Ile Ser Ile Leu Ala Ala Gly Glu320 325 330TGT AGT CTT TCA GCA GAA GCA GGG GAC ATT ACC TTC AAT GGG AAT GCC 1299Cys Ser Leu Ser Ala Glu Ala Gly Asp Ile Thr Phe Asn Gly Asn Ala335 340 345ATT GTT GCA ACT ACA CCA CAA ACT ACA AAA AGA AAT TCT ATT GAC ATA 1347Ile Val Ala Thr Thr Pro Gln Thr Thr Lys Arg Asn Ser Ile Asp Ile350 355 360GGA TCT ACT GCA AAG ATC ACG AAT TTA CGT GCA ATA TCT GGG CAT AGC 1395Gly Ser Thr Ala Lys Ile Thr Asn Leu Arg Ala Ile Ser Gly His Ser365 370 375ATC TTT TTC TAC GAT CCG ATT ACT GCT AAT ACG GCT GCG GAT TCT ACA 1443Ile Phe Phe Tyr Asp Pro Ile Thr Ala Asn Thr Ala Ala Asp Ser Thr380 385 390 395GAT ACT TTA AAT CTC AAT AAG GCT GAT GCA GGT AAT AGT ACA GAT TAT 1491Asp Thr Leu Asn Leu Asn Lys Ala Asp Ala Gly Asn Ser Thr Asp Tyr400 405 410AGT GGG TCG ATT GTT TTT TCT GGT GAA AAG CTC TCT GAA GAT GAA GCA 1539Ser Gly Ser Ile Val Phe Ser Gly Glu Lys Leu Ser Glu Asp Glu Ala415 420 425AAA GTT GCA GAC AAC CTC ACT TCT ACG CTG AAG CAG CCT GTA ACT CTA 1587Lys Val Ala Asp Asn Leu Thr Ser Thr Leu Lys Gln Pro Val Thr Leu430 435 440ACT GCA GGA AAT TTA GTA CTT AAA CGT GGT GTC ACT CTC GAT ACG AAA 1635Thr Ala Gly Asn Leu Val Leu Lys Arg Gly Val Thr Leu Asp Thr Lys445 450 455GGC TTT ACT CAG ACC GCG GGT TCC TCT GTT ATT ATG GAT GCG GGC ACA 1683Gly Phe Thr Gln Thr Ala Gly Ser Ser Val Ile Met Asp Ala Gly Thr460 465 470 475ACG TTA AAA GCA AGT ACA GAG GAG GTC ACT TTA ACA GGT CTT TCC ATT 1731Thr Leu Lys Ala Ser Thr Glu Glu Val Thr Leu Thr Gly Leu Ser Ile480 485 490CCT GTA GAC TCT TTA GGC GAG GGT AAG AAA GTT GTA ATT GCT GCT TCT 1779Pro Val Asp Ser Leu Gly Glu Gly Lys Lys Val Val Ile Ala Ala Ser495 500 505GCA GCA AGT AAA AAT GTA GCC CTT AGT GGT CCG ATT CTT CTT TTG GAT 1827Ala Ala Ser Lys Asn Val Ala Leu Ser Gly Pro Ile Leu Leu Leu Asp510 515 520AAC CAA GGG AAT GCT TAT GAA AAT CAC GAC TTA GGA AAA ACT CAA GAC 1875Asn Gln Gly Asn Ala Tyr Glu Asn His Asp Leu Gly Lys Thr Gln Asp525 530 535TTT TCA TTT GTG CAG CTC TCT GCT CTG GGT ACT GCA ACA ACT ACA GAT 1923Phe Ser Phe Val Gln Leu Ser Ala Leu Gly Thr Ala Thr Thr Thr Asp540 545 550 555GTT CCA GCG GTT CCT ACA GTA GCA ACT CCT ACG CAC TAT GGG TAT CAA 1971Val Pro Ala Val Pro Thr Val Ala Thr Pro Thr His Tyr Gly Tyr Gln560 565 570GGT ACT TGG GGA ATG ACT TGG GTT GAT GAT ACC GCA AGC ACT CCA AAG 2019Gly Thr Trp Gly Met Thr Trp Val Asp Asp Thr Ala Ser Thr Pro Lys575 580 585ACT AAG ACA GCG ACA TTA GCT TGG ACC AAT ACA GGC TAC CTT CCG AAT 2067Thr Lys Thr Ala Thr Leu Ala Trp Thr Asn Thr Gly Tyr Leu Pro Asn590 595 600CCT GAG CGT CAA GGA CCT TTA GTT CCT AAT AGC CTT TGG GGA TCT TTT 2115Pro Glu Arg Gln Gly Pro Leu Val Pro Asn Ser Leu Trp Gly Ser Phe605 610 615TCA GAC ATC CAA GCG ATT CAA GGT GTC ATA GAG AGA AGT GCT TTG ACT 2163Ser Asp Ile Gln Ala Ile Gln Gly Val Ile Glu Arg Ser Ala Leu Thr620 625 630 635CTT TGT TCA GAT CGA GGC TTC TGG GCT GCG GGA GTC GCC AAT TTC TTA 2211Leu Cys Ser Asp Arg Gly Phe Trp Ala Ala Gly Val Ala Asn Phe Leu640 645 650GAT AAA GAT AAG AAA GGG GAA AAA CGC AAA TAC CGT CAT AAA TCT GGT 2259Asp Lys Asp Lys Lys Gly Glu Lys Arg Lys Tyr Arg His Lys Ser Gly655 660 665GGA TAT GCT ATC GGA GGT GCA GCG CAA ACT TGT TCT GAA AAC TTA ATT 2307Gly Tyr Ala Ile Gly Gly Ala Ala Gln Thr Cys Ser Glu Asn Leu Ile670 675 680AGC TTT GCC TTT TGC CAA CTC TTT GGT AGC GAT AAA GAT TTC TTA GTC 2355Ser Phe Ala Phe Cys Gln Leu Phe Gly Ser Asp Lys Asp Phe Leu Val685 690 695GCT AAA AAT CAT ACT GAT ACC TAT GCA GGA GCC TTC TAT ATC CAA CAC 2403Ala Lys Asn His Thr Asp Thr Tyr Ala Gly Ala Phe Tyr Ile Gln His700 705 710 715ATT ACA GAA TGT AGT GGG TTC ATA GGT TGT CTC TTA GAT AAA CTT CCT 2451Ile Thr Glu Cys Ser Gly Phe Ile Gly Cys Leu Leu Asp Lys Leu Pro720 725 730GGC TCT TGG AGT CAT AAA CCC CTC GTT TTA GAA GGG CAG CTC GCT TAT 2499Gly Ser Trp Ser His Lys Pro Leu Val Leu Glu Gly Gln Leu Ala Tyr735 740 745AGC CAC GTC AGT AAT GAT CTG AAG ACA AAG TAT ACT GCG TAT CCT GAG 2547Ser His Val Ser Asn Asp Leu Lys Thr Lys Tyr Thr Ala Tyr Pro Glu750 755 760GTG AAA GGT TCT TGG GGG AAT AAT GCT TTT AAC ATG ATG TTG GGA GCT 2595Val Lys Gly Ser Trp Gly Asn Asn Ala Phe Asn Met Met Leu Gly Ala765 770 775TCT TCT CAT TCT TAT CCT GAA TAC CTG CAT TGT TTT GAT ACC TAT GCT 2643Ser Ser His Ser Tyr Pro Glu Tyr Leu His Cys Phe Asp Thr Tyr Ala780 785 790 795CCA TAC ATC AAA CTG AAT CTG ACC TAT ATA CGT CAG GAC AGC TTC TCG 2691Pro Tyr Ile Lys Leu Asn Leu Thr Tyr Ile Arg Gln Asp Ser Phe Ser800 805 810GAG AAA GGT ACA GAA GGA AGA TCT TTT GAT GAC AGC AAC CTC TTC AAT 2739Glu Lys Gly Thr Glu Gly Arg Ser Phe Asp Asp Ser Asn Leu Phe Asn815 820 825TTA TCT TTG CCT ATA GGG GTG AAG TTT GAG AAG TTC TCT GAT TGT AAT 2787Leu Ser Leu Pro Ile Gly Val Lys Phe Glu Lys Phe Ser Asp Cys Asn830 835 840GAC TTT TCT TAT GAT CTG ACT TTA TCC TAT GTT CCT GAT CTT ATC CGC 2835Asp Phe Ser Tyr Asp Leu Thr Leu Ser Tyr Val Pro Asp Leu Ile Arg845 850 855AAT GAT CCC AAA TGC ACT ACA GCA CTT GTA ATC AGC GGA GCC TCT TGG 2883Asn Asp Pro Lys Cys Thr Thr Ala Leu Val Ile Ser Gly Ala Ser Trp860 865 870 875GAA ACT TAT GCC AAT AAC TTA GCA CGA CAG GCC TTG CAA GTG CGT GCA 2931Glu Thr Tyr Ala Asn Asn Leu Ala Arg Gln Ala Leu Gln Val Arg Ala880 885 890GGC AGT CAC TAC GCC TTC TCT CCT ATG TTT GAA GTG CTC GGC CAG TTT 2979Gly Ser His Tyr Ala Phe Ser Pro Met Phe Glu Val Leu Gly Gln Phe895 900 905GTC TTT GAA GTT CGT GGA TCC 3000Val Phe Glu Val Arg Gly Ser910(2)SEQ ID NO26的信息(i)序列特征(A)長(zhǎng)度914氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型蛋白質(zhì)(v)片段類(lèi)型內(nèi)部(xi)序列描述SEQ ID NO26Met Lys Ser Gln Phe Ser Trp Leu Val Leu Ser Ser Thr Leu Ala Cys1 5 10 15Phe Thr Ser Cys Ser Thr Val Phe Ala Ala Thr Ala Glu Asn Ile Gly20 25 30Pro Ser Asp Ser Phe Asp Gly Ser Thr Asn Thr Gly Thr Tyr Thr Pro35 40 45Lys Asn Thr Thr Thr Gly Ile Asp Tyr Thr Leu Thr Gly Asp Ile Thr50 55 60Leu Gln Asn Leu Gly Asp Ser Ala Ala Leu Thr Lys Gly Cys Phe Ser65 70 75 80Asp Thr Thr Glu Ser Leu Ser Phe Ala Gly Lys Gly Tyr Ser Leu Ser85 90 95Phe Leu Asn Ile Lys Ser Ser Ala Glu Gly Ala Ala Leu Ser Val Thr100 105 110Thr Asp Lys Asn Leu Ser Leu Thr Gly Phe Ser Ser Leu Thr Phe Leu115 120 125Ala Ala Pro Ser Ser Val Ile Thr Thr Pro Ser Gly Lys Gly Ala Val130 135 140Lys Cys Gly Gly Asp Leu Thr Phe Asp Asn Asn Gly Thr Ile Leu Phe145 150 155 160Lys Gln Asp Tyr Cys Glu Glu Asn Gly Gly Ala Ile Ser Thr Lys Asn165 170 175Leu Ser Leu Lys Asn Ser Thr Gly Ser Ile Ser Phe Glu Gly Asn Lys180 185 190Ser Ser Ala Thr Gly Lys Lys Gly Gly Ala Ile Cys Ala Thr Gly Thr195 200 205Val Asp Ile Thr Asn Asn Thr Ala Pro Thr Leu Phe Ser Asn Asn Ile210 215 220Ala Glu Ala Ala Gly Gly Ala Ile Asn Ser Thr Gly Asn Cys Thr Ile225 230 235 240Thr Gly Asn Thr Ser Leu Val Phe Ser Glu Asn Ser Val Thr Ala Thr245 250 255Ala Gly Asn Gly Gly Ala Leu Ser Gly Asp Ala Asp Val Thr Ile Ser
260 265 270Gly Asn Gln Ser Val Thr Phe Ser Gly Asn Gln Ala Val Ala Asn Gly275 280 285Gly Ala Ile Tyr Ala Lys Lys Leu Thr Leu Ala Ser Gly Gly Gly Gly290 295 300Gly Ile Ser Phe Ser Asn Asn Ile Val Gln Gly Thr Thr Ala Gly Asn305 310 315 320Gly Gly Ala Ile Ser Ile Leu Ala Ala Gly Glu Cys Ser Leu Ser Ala325 330 335Glu Ala Gly Asp Ile Thr Phe Asn Gly Asn Ala Ile Val Ala Thr Thr340 345 350Pro Gln Thr Thr Lys Arg Asn Ser Ile Asp Ile Gly Ser Thr Ala Lys355 360 365Ile Thr Asn Leu Arg Ala Ile Ser Gly His Ser Ile Phe Phe Tyr Asp370 375 380Pro Ile Thr Ala Asn Thr Ala Ala Asp Ser Thr Asp Thr Leu Asn Leu385 390 395 400Asn Lys Ala Asp Ala Gly Asn Ser Thr Asp Tyr Ser Gly Ser Ile Val405 410 415Phe Ser Gly Glu Lys Leu Ser Glu Asp Glu Ala Lys Val Ala Asp Asn420 425 430Leu Thr Ser Thr Leu Lys Gln Pro ValThr Leu Thr Ala Gly Asn Leu435 440445Val Leu Lys Arg Gly Val Thr Leu Asp Thr Lys Gly Phe Thr Gln Thr450 455 460Ala Gly Ser Ser Val Ile Met Asp Ala Gly Thr Thr Leu Lys Ala Ser465 470 475 480Thr Glu Glu Val Thr Leu Thr Gly Leu Ser Ile Pro Val Asp Ser Leu485 490 495Gly Glu Gly Lys Lys Val Val Ile Ala Ala Ser Ala Ala Ser Lys Asn500 505 510Val Ala Leu Ser Gly Pro Ile Leu Leu Leu Asp Asn Gln Gly Asn Ala515 520 525Tyr Glu Asn His Asp Leu Gly Lys Thr Gln Asp Phe Ser Phe Val Gln530 535 540Leu Ser Ala Leu Gly Thr Ala Thr Thr Thr Asp Val Pro Ala Val Pro545 550 555 560Thr Val Ala Thr Pro Thr His Tyr Gly Tyr Gln Gly Thr Trp Gly Met565 570 575Thr Trp Val Asp Asp Thr Ala Ser Thr Pro Lys Thr Lys Thr Ala Thr580 585 590Leu Ala Trp Thr Asn Thr Gly Tyr Leu Pro Asn Pro Glu Arg Gln Gly595 600 605Pro Leu Val Pro Asn Ser Leu Trp Gly Ser Phe Ser Asp Ile Gln Ala610 615 620Ile Gln Gly Val Ile Glu Arg Ser Ala Leu Thr Leu Cys Ser Asp Arg625 630 635 640Gly Phe Trp Ala Ala Gly Val Ala Asn Phe Leu Asp Lys Asp Lys Lys645 650 655Gly Glu Lys Arg Lys Tyr Arg His Lys Ser Gly Gly Tyr Ala Ile Gly660 665 670Gly Ala Ala Gln Thr Cys Ser Glu Asn Leu Ile Ser Phe Ala Phe Cys
675 680 685Gln Leu Phe Gly Ser Asp Lys Asp Phe Leu Val Ala Lys Asn His Thr690 695 700Asp Thr Tyr Ala Gly Ala Phe Tyr Ile Gln His Ile Thr Glu Cys Ser705 710 715 720Gly Phe Ile Gly Cys Leu Leu Asp Lys Leu Pro Gly Ser Trp Ser His725 730 735Lys Pro Leu Val Leu Glu Gly Gln Leu Ala Tyr Ser His Val Ser Asn740 745 750Asp Leu Lys Thr Lys Tyr Thr Ala Tyr Pro Glu Val Lys Gly Ser Trp755 760 765Gly Asn Asn Ala Phe Asn Met Met Leu Gly Ala Ser Ser His Ser Tyr770 775 780Pro Glu Tyr Leu His Cys Phe Asp Thr Tyr Ala Pro Tyr Ile Lys Leu785 790 795 800Asn Leu Thr Tyr Ile Arg Gln Asp Ser Phe Ser Glu Lys Gly Thr Glu805 810 815Gly Arg Ser Phe Asp Asp Ser Asn Leu Phe Asn Leu Ser Leu Pro Ile820 825 830Gly Val Lys Phe Glu Lys Phe Ser Asp Cys Asn Asp Phe Ser Tyr Asp835 840 845Leu Thr Leu Ser Tyr Val Pro Asp Leu Ile Arg Asn Asp Pro Lys Cys850 855 860Thr Thr Ala Leu Val Ile Ser Gly Ala Ser Trp Glu Thr Tyr Ala Asn865 870 875 880Asn Leu Ala Arg Gln Ala Leu Gln Val Arg Ala Gly Ser His Tyr Ala885 890 895Phe Ser Pro Met Phe Glu Val Leu Gly Gln Phe Val Phe Glu Val Arg900 905 910Gly Ser(2)SEQ ID N027的信息(i)序列特征(A)長(zhǎng)度1200堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ix)特征(A)名稱/關(guān)鍵字編碼序列(B)位置1...1200(D)其它信息(xi)序列描述SEQ ID NO27GAT CCT AAA AAT AAA GAG TAC ACA GGG ACC ATA CTC TTT TCT GGA GAA 48Asp Pro Lys Asn Lys Glu Tyr Thr Gly Thr Ile Leu Phe Ser Gly Glu1 5 10 15AAG AGT CTA GCA AAC GAT CCT AGG GAT TTT AAA TCT ACA ATC CCT CAG 96Lys Ser Leu Ala Asn Asp Pro Arg Asp Phe Lys Ser Thr Ile Pro Gln20 25 30AAC GTC AAC CTG TCT GCA GGA TAC TTA GTT ATT AAA GAG GGG GCC GAA 144Asn Val Asn Leu Ser Ala Gly Tyr Leu Val Ile Lys Glu Gly Ala Glu35 40 45GTC ACA GTT TCA AAA TTC ACG CAG TCT CCA GGA TCG CAT TTA GTT TTA 192Val Thr Val Ser Lys Phe Thr Gln Ser Pro Gly Ser His Leu Val Leu50 55 60GAT TTA GGA ACC AAA CTG ATA GCC TCT AAG GAA GAC ATT GCC ATC ACA 240Asp Leu Gly Thr Lys Leu Ile Ala Ser Lys Glu Asp Ile Ala Ile Thr65 70 75 80GGC CTC GCG ATA GAT ATA GAT AGC TTA AGC TCA TCC TCA ACA GCA GCT 288Gly Leu Ala Ile Asp Ile Asp Ser Leu Ser Ser Ser Ser Thr Ala Ala85 90 95GTT ATT AAA GCA AAC ACC GCA AAT AAA CAG ATA TCC GTG ACG GAC TCT 336Val Ile Lys Ala Asn Thr Ala Asn Lys Gln Ile Ser Val Thr Asp Ser100 105 110ATA GAA CTT ATC TCG CCT ACT GGC AAT GCC TAT GAA GAT CTC AGA ATG 384Ile Glu Leu Ile Ser Pro Thr Gly Asn Ala Tyr Glu Asp Leu Arg Met115 120 125AGA AAT TCA CAG ACG TTC CCT CTG CTC TCT TTA GAG CCT GGA GCC GGG 432Arg Asn Ser Gln Thr Phe Pro Leu Leu Ser Leu Glu Pro Gly Ala Gly130 135 140GGT AGT GTG ACT GTA ACT GCT GGA GAT TTC CTA CCG GTA AGT CCC CAT 480Gly Ser Val Thr Val Thr Ala Gly Asp Phe Leu Pro Val Ser Pro His145 150 155 160TAT GGT TTT CAA GGC AAT TGG AAA TTA GCT TGG ACA GGA ACT GGA AAC 528Tyr Gly Phe Gln Gly Asn Trp Lys Leu Ala Trp Thr Gly Thr Gly Asn165 170 175AAA GTT GGA GAA TTC TTC TGG GAT AAA ATA AAT TAT AAG CCT AGA CCT 576Lys Val Gly Glu Phe Phe Trp Asp Lys Ile Asn Tyr Lys Pro Arg Pro180 185 190GAA AAA GAA GGA AAT TTA GTT CCT AAT ATC TTG TGG GGG AAT GCT GTA 624Glu Lys Glu Gly Asn Leu Val Pro Asn Ile Leu Trp Gly Asn Ala Val195 200 205AAT GTC AGA TCC TTA ATG CAG GTT CAA GAG ACC CAT GCA TCG AGC TTA 672Asn Val Arg Ser Leu Met Gln Val Gln Glu Thr His Ala Ser Ser Leu210 215 220CAG ACA GAT CGA GGG CTG TGG ATC GAT GGA ATT GGG AAT TTC TTC CAT 720Gln Thr Asp Arg Gly Leu Trp Ile Asp Gly Ile Gly Asn Phe Phe His225 230 235 240GTA TCT GCC TCC GAA GAC AAT ATA AGG TAC CGT CAT AAC AGC GGT GGA 768Val Ser Ala Ser Glu Asp Asn Ile Arg Tyr Arg His Asn Ser Gly Gly245 250 255TAT GTT CTA TCT GTA AAT AAT GAG ATC ACA CCT AAG CAC TAT ACT TCG 816Tyr Val Leu Ser Val Asn Asn Glu Ile Thr Pro Lys His Tyr Thr Ser260 265 270ATG GCA TTT TCC CAA CTC TTT AGT AGA GAC AAA GAC TAT GCG GTT TCC 864Met Ala Phe Ser Gln Leu Phe Ser Arg Asp Lys Asp Tyr Ala Val Ser275 280 285AAC AAC GAA TAC AGA ATG TAT TTA GGA TCG TAT CTC TAT CAA TAT ACA 912Asn Asn Glu Tyr Arg Met Tyr Leu Gly Ser Tyr Leu Tyr Gln Tyr Thr290 295 300ACC TCC CTA GGG AAT ATT TTC CGT TAT GCT TCG CGT AAC CCT AAT GTA 960Thr Ser Leu Gly Asn Ile Phe Arg Tyr Ala Ser Arg Asn Pro Asn Val305 310 315 320AAC GTC GGG ATT CTC TCA AGA AGG TTT CTT CAA AAT CCT CTT ATG ATT 1008Asn Val Gly Ile Leu Ser Arg Arg Phe Leu Gln Asn Pro Leu Met Ile325 330 335TTT CAT TTT TTG TGT GCT TAT GGT CAT GCC ACC AAT GAT ATG AAA ACA 1056Phe His Phe Leu Cys Ala Tyr Gly His Ala Thr Asn Asp Met Lys Thr340 345 350GAC TAC GCA AAT TTC CCT ATG GTG AAA AAC AGC TGG AGA AAC AAT TGT 1104Asp Tyr Ala Asn Phe Pro Met Val Lys Asn Ser Trp Arg Asn Asn Cys355 360 365TGG GCT ATA AAA TGC GGA GGG AGC ATG CCT CTA TTG GTA TTT GAA AAC 1152Trp Ala Ile Lys Cys Gly Gly Ser Met Pro Leu Leu Val Phe Glu Asn370 375 380GGA AAA CTT TTC CAA GGT GCC ATC CCA TTT ATG AAA CTA CAA TTA GTT 1200Gly Lys Leu Phe Gln Gly Ala Ile Pro Phe Met Lys Leu Gln Leu Val385 390 395 400(2)SEQ ID NO28的信息(i)序列特征(A)長(zhǎng)度400氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性
(ii)分子類(lèi)型蛋白質(zhì)(v)片段類(lèi)型內(nèi)部(xi)序列描述SEQ ID NO28Asp Pro Lys Asn Lys Glu Tyr Thr Gly Thr Ile Leu Phe Ser Gly Glu1 5 10 15Lys Ser Leu Ala Asn Asp Pro Arg Asp Phe Lys Ser Thr Ile Pro Gln20 25 30Asn Val Asn Leu Ser Ala Gly Tyr Leu Val Ile Lys Glu Gly Ala Glu35 40 45Val Thr Val Ser Lys Phe Thr Gln Ser Pro Gly Ser His Leu Val Leu50 55 60Asp Leu Gly Thr Lys Leu Ile Ala Ser Lys Glu Asp Ile Ala Ile Thr65 70 75 80Gly Leu Ala Ile Asp Ile Asp Ser Leu Ser Ser Ser Ser Thr Ala Ala85 90 95Val Ile Lys Ala Asn Thr Ala Asn Lys Gln Ile Ser Val Thr Asp Ser100 105 110Ile Glu Leu Ile Ser Pro Thr Gly Asn Ala Tyr Glu Asp Leu Arg Met115 120 125Arg Asn Ser Gln Thr Phe Pro Leu Leu Ser Leu Glu Pro Gly Ala Gly130 135 140Gly Ser Val Thr Val Thr Ala Gly Asp Phe Leu Pro Val Ser Pro His145 150 155 160Tyr Gly Phe Gln Gly Asn Trp Lys Leu Ala Trp Thr Gly Thr Gly Asn165 170 175Lys Val Gly Glu Phe Phe Trp Asp Lys Ile Asn Tyr Lys Pro Arg Pro180 185 190Glu Lys Glu Gly Asn Leu Val Pro Asn Ile Leu Trp Gly Asn Ala Val195 200 205Asn Val Arg Ser Leu Met Gln Val Gln Glu Thr His Ala Ser Ser Leu210 215 220Gln Thr Asp Arg Gly Leu Trp Ile Asp Gly Ile Gly Asn Phe Phe His225 230 235 240Val Ser Ala Ser Glu Asp Asn Ile Arg Tyr Arg His Asn Ser Gly Gly245 250 255Tyr Val Leu Ser Val Asn Asn Glu Ile Thr Pro Lys His Tyr Thr Ser260 265 270Met Ala Phe Ser Gln Leu Phe Ser Arg Asp Lys Asp Tyr Ala Val Ser275 280 285Asn Asn Glu Tyr Arg Met Tyr Leu Gly Ser Tyr Leu Tyr Gln Tyr Thr290 295 300Thr Ser Leu Gly Asn Ile Phe Arg Tyr Ala Ser Arg Asn Pro Asn Val305 310 315 320Asn Val Gly Ile Leu Ser Arg Arg Phe Leu Gln Asn Pro Leu Met Ile325 330 335Phe His Phe Leu Cys Ala Tyr Gly His Ala Thr Asn Asp Met Lys Thr340 345 350Asp Tyr Ala Asn Phe Pro Met Val Lys Asn Ser Trp Arg Asn Asn Cys355 360 365Trp Ala Ile Lys Cys Gly Gly Ser Met Pro Leu Leu Val Phe Glu Asn370 375 380Gly Lys Leu Phe Gln Gly Ala Ile Pro Phe Met Lys Leu Gln Leu Val385 390 395 400(2)SEQ ID NO29的信息(i)序列特征(A)長(zhǎng)度1830堿基對(duì)(B)類(lèi)型核酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型cDNA(ix)特征(A)名稱/關(guān)鍵字編碼序列(B)位置1...1830(D)其它信息(xi)序列描述SEQ ID NO29GAT CTC ACA TTA GGG AGT CGT GAC AGT TAT AAT GGT GAT ACA AGC ACC 48Asp Leu Thr Leu Gly Ser Arg Asp Ser Tyr Asn Gly Asp Thr Ser Thr1 5 10 15ACA GAA TTT ACT CCT AAA GCG GCA ACT TCT GAT GCT AGT GGC ACG ACC 96Thr Glu Phe Thr Pro Lys Ala Ala Thr Ser Asp Ala Ser Gly Thr Thr20 25 30TAT ATT CTC GAT GGG GAT GTC TCG ATA AGC CAA GCA GGG AAA CAA ACG 144Tyr Ile Leu Asp Gly Asp Val Ser Ile Ser Gln Ala Gly Lys Gln Thr35 40 45AGC TTA ACC ACA AGT TGT TTT TCT AAC ACT GCA GGA AAT CTT ACC TTC 192Ser Leu Thr Thr Ser Cys Phe Ser Asn Thr Ala Gly Asn Leu Thr Phe50 55 60TTA GGG AAC GGA TTT TCT CTT CAT TTT GAC AAT ATT ATT TCG TCT ACT 240Leu Gly Asn Gly Phe Ser Leu His Phe Asp Asn Ile Ile Ser Ser Thr65 70 75 80GTT GCA GGT GTT GTT GTT AGC AAT ACA GCA GCT TCT GGG ATT ACG AAA 288Val Ala Gly Val Val Val Ser Asn Thr Ala Ala Ser Gly Ile Thr Lys85 90 95TTC TCA GGA TTT TCA ACT CTT CGG ATG CTT GCA GCT CCT AGG ACC ACA 336Phe Ser Gly Phe Ser Thr Leu Arg Met Leu Ala Ala Pro Arg Thr Thr100 105 110GGT AAA GGA GCC ATT AAA ATT ACC GAT GGT CTG GTG TTT GAG AGT ATA 384Gly Lys Gly Ala Ile Lys Ile Thr Asp Gly Leu Val Phe Glu Ser Ile
115 120 125GGG AAT CTT GAT CCG ATT ACT GTA ACA GGA TCG ACA TCT GTT GCT GAT 432Gly Asn Leu Asp Pro Ile Thr Val Thr Gly Ser Thr Ser Val Ala Asp130 135 140GCT CTC AAT ATT AAT AGC CCT GAT ACT GGA GAT AAC AAA GAG TAT ACG 480Ala Leu Asn Ile Asn Ser Pro Asp Thr Gly Asp Asn Lys Glu Tyr Thr145 150 155 160GGA ACC ATA GTC TTT TCT GGA GAG AAG CTC ACG GAG GCA GAA GCT AAA 528Gly Thr Ile Val Phe Ser Gly Glu Lys Leu Thr Glu Ala Glu Ala Lys165 170 175GAT GAG AAG AAC CGC ACT TCT AAA TTA CTT CAA AAT GTT GCT TTT AAA 576Asp Glu Lys Asn Arg Thr Ser Lys Leu Leu Gln Asn Val Ala Phe Lys180 185 190AAT GGG ACT GTA GTT TTA AAA GGT GAT GTC GTT TTA AGT GCG AAC GGT 624Asn Gly Thr Val Val Leu Lys Gly Asp Val Val Leu Ser Ala Asn Gly195 200 205TTC TCT CAG GAT GCA AAC TCT AAG TTG ATT ATG GAT TTA GGG ACG TCG 672Phe Ser Gln Asp Ala Asn Ser Lys Leu Ile Met Asp Leu Gly Thr Ser210 215 220TTG GTT GCA AAC ACC GAA AGT ATC GAG TTA ACG AAT TTG GAA ATT AAT 720Leu Val Ala Asn Thr Glu Ser Ile Glu Leu Thr Asn Leu Glu Ile Asn225 230 235 240ATA GAC TCT CTC AGG AAC GGG AAA AAG ATA AAA CTC AGT GCT GCC ACA 768Ile Asp Ser Leu Arg Asn Gly Lys Lys Ile Lys Leu Ser Ala Ala Thr245 250 255GCT CAG AAA GAT ATT CGT ATA GAT CGT CCT GTT GTA CTG GCA ATT AGC 816Ala Gln Lys Asp Ile Arg Ile Asp Arg Pro Val Val Leu Ala Ile Ser260 265 270GAT GAG AGT TTT TAT CAA AAT GGC TTT TTG AAT GAG GAC CAT TCC TAT 864Asp Glu Ser Phe Tyr Gln Asn Gly Phe Leu Asn Glu Asp His Ser Tyr275 280 285GAT GGG ATT CTT GAG TTA GAT GCT GGG AAA GAC ATC GTG ATT TCT GCA 912Asp Gly Ile Leu Glu Leu Asp Ala Gly Lys Asp Ile Val Ile Ser Ala290 295 300GAT TCT CGC AGT ATA GAT GCT GTA CAA TCT CCG TAT GGC TAT CAG GGA 960Asp Ser Arg Ser Ile Asp Ala Val Gln Ser Pro Tyr Gly Tyr Gln Gly305 310 315 320AAG TGG ACG ATC AAT TGG TCT ACT GAT GAT AAG AAA GCT ACG GTT TCT 1008Lys Trp Thr Ile Asn Trp Ser Thr Asp Asp Lys Lys Ala Thr Val Ser
325 330 335TGG GCG AAG CAG AGT TTT AAT CCC ACT GCT GAG CAG GAG GCT CCG TTA 1056Trp Ala Lys Gln Ser Phe Asn Pro Thr Ala Glu Gln Glu Ala Pro Leu340 345 350GTT CCT AAT CTT CTT TGG GGT TCT TTT ATA GAT GTT CGT TCC TTC CAG 1104Val Pro Asn Leu Leu Trp Gly Ser Phe Ile Asp Val Arg Ser Phe Gln355 360 365AAT TTT ATA GAG CTA GGT ACT GAA GGT GCT CCT TAC GAA AAG AGA TTT 1152Asn Phe Ile Glu Leu Gly Thr Glu Gly Ala Pro Tyr Glu Lys Arg Phe370 375 380TGG GTT GCA GGC ATT TCC AAT GTT TTG CAT AGG AGC GGT CGT GAA AAT 1200Trp Val Ala Gly Ile Ser Asn Val Leu His Arg Ser Gly Arg Glu Asn385 390 395 400CAA AGG AAA TTC CGT CAT GTG AGT GGA GGT GCT GTA GTA GGT GCT AGC 1248Gln Arg Lys Phe Arg His Val Ser Gly Gly Ala Val Val Gly Ala Ser405 410 415ACG AGG ATG CCG GGT GGT GAT ACC TTG TCT CTG GGT TTT GCT CAG CTC 1296Thr Arg Met Pro Gly Gly Asp Thr Leu Ser Leu Gly Phe Ala Gln Leu420 425 430TTT GCG CGT GAC AAA GAC TAC TTT ATG AAT ACC AAT TTC GCA AAG ACC 1344Phe Ala Arg Asp Lys Asp Tyr Phe Met Asn Thr Asn Phe Ala Lys Thr435 440 445TAC GCA GGA TCT TTA CGT TTG CAG CAC GAT GCT TCC CTA TAC TCT GTG 1392Tyr Ala Gly Ser Leu Arg Leu Gln His Asp Ala Ser Leu Tyr Ser Val450 455 460GTG AGT ATC CTT TTA GGA GAG GGA GGA CTC CGC GAG ATC CTG TTG CCT 1440Val Ser Ile Leu Leu Gly Glu Gly Gly Leu Arg Glu Ile Leu Leu Pro465 470 475 480TAT GTT TCC AAT ACT CTG CCG TGC TCT TTC TAT GGG CAG CTT AGC TAC 1488Tyr Val Ser Asn Thr Leu Pro Cys Ser Phe Tyr Gly Gln Leu Ser Tyr485 490 495GGC CAT ACG GAT CAT CGC ATG AAG ACC GAG TCT CTA CCC CCC CCC CCC 1536Gly His Thr Asp His Arg Met Lys Thr Glu Ser Leu Pro Pro Pro Pro500 505 510CCG ACG CTC TCG ACG GAT CAT ACT TCT TGG GGA GGA TAT GTC TGG GCT 1584Pro Thr Leu Ser Thr Asp His Thr Ser Trp Gly Gly Tyr Val Trp Ala515 520 525GGA GAG CTG GGA ACT CGA GTT GCT GTT GAA AAT ACC AGC GGC AGA GGA 1632Gly Glu Leu Gly Thr Arg Val Ala Val Glu Asn Thr Ser Gly Arg Gly
530 535 540TTT TTC CGA GAG TAC ACT CCA TTT GTA AAA GTC CAA GCT GTT TAC TCG 1680Phe Phe Arg Glu Tyr Thr Pro Phe Val Lys Val Gln Ala Val Tyr Ser545 550 555 560CGC CAA GAT AGC TTT GTT GAA CTA GGA GCT ATC AGT CGT GAT TTT AGT 1728Arg Gln Asp Ser Phe Val Glu Leu Gly Ala Ile Ser Arg Asp Phe Ser565 570 575GAT TCG CAT CTT TAT AAC CTT GCG ATT CCT CTT GGA ATC AAG TTA GAG 1776Asp Ser His Leu Tyr Asn Leu Ala Ile Pro Leu Gly Ile Lys Leu Glu580 585 590AAA CGG TTT GCA GAG CAA TAT TAT CAT GTT GTT GCG ATG TAT TCT CCA 1824Lys Arg Phe Ala Glu Gln Tyr Tyr His Val Val Ala Met Tyr Ser Pro595 600 605GAT GTT 1830Asp Val610(2)SEQ ID NO30的信息(i)序列特征(A)長(zhǎng)度610氨基酸(B)類(lèi)型氨基酸(C)鏈型單鏈(D)拓?fù)浣Y(jié)構(gòu)線性(ii)分子類(lèi)型蛋白質(zhì)(v)片段類(lèi)型內(nèi)部(xi)序列描述SEQ ID NO30Asp Leu Thr Leu Gly Ser Arg Asp Ser Tyr Asn Gly Asp Thr Ser Thr1 5 10 15Thr Glu Phe Thr Pro Lys Ala Ala Thr Ser Asp Ala Ser Gly Thr Thr20 25 30Tyr Ile Leu Asp Gly Asp Val Ser Ile Ser Gln Ala Gly Lys Gln Thr35 40 45Ser Leu Thr Thr Ser Cys Phe Ser Asn Thr Ala Gly Asn Leu Thr Phe50 55 60Leu Gly Asn Gly Phe Ser Leu His Phe Asp Asn Ile Ile Ser Ser Thr65 70 75 80Val Ala Gly Val Val Val Ser Asn Thr Ala Ala Ser Gly Ile Thr Lys85 90 95Phe Ser Gly Phe Ser Thr Leu Arg Met Leu Ala Ala Pro Arg Thr Thr100 105 110Gly Lys Gly Ala Ile Lys Ile Thr Asp Gly Leu Val Phe Glu Ser Ile115 120 125Gly Asn Leu Asp Pro Ile Thr Val Thr Gly Ser Thr Ser Val Ala Asp130 135 140Ala Leu Asn Ile Asn Ser Pro Asp Thr Gly Asp Asn Lys Glu Tyr Thr145 150 155 160Gly Thr Ile Val Phe Ser Gly Glu Lys Leu Thr Glu Ala Glu Ala Lys165 170 175Asp Glu Lys Asn Arg Thr Ser Lys Leu Leu Gln Asn Val Ala Phe Lys180 185 190Asn Gly Thr Val Val Leu Lys Gly Asp Val Val Leu Ser Ala Asn Gly195 200 205Phe Ser Gln Asp Ala Asn Ser Lys Leu Ile Met Asp Leu Gly Thr Ser210 215 220Leu Val Ala Asn Thr Glu Ser Ile Glu Leu Thr Asn Leu Glu Ile Asn225 230 235 240Ile Asp Ser Leu Arg Asn Gly Lys Lys Ile Lys Leu Ser Ala Ala Thr245 250 255Ala Gln Lys Asp Ile Arg Ile Asp Arg Pro Val Val Leu Ala Ile Ser260 265 270Asp Glu Ser Phe Tyr Gln Asn Gly Phe Leu Asn Glu Asp His Ser Tyr275 280 285Asp Gly Ile Leu Glu Leu Asp Ala Gly Lys Asp Ile Val Ile Ser Ala290 295 300Asp Ser Arg Ser Ile Asp Ala Val Gln Ser Pro Tyr Gly Tyr Gln Gly305 310 315 320Lys Trp Thr Ile Asn Trp Ser Thr Asp Asp Lys Lys Ala Thr Val Ser325 330 335Trp Ala Lys Gln Ser Phe Asn Pro Thr Ala Glu Gln Glu Ala Pro Leu340 345 350Val Pro Asn Leu Leu Trp Gly Ser Phe Ile Asp Val Arg Ser Phe Gln355 360 365Asn Phe Ile Glu Leu Gly Thr Glu Gly Ala Pro Tyr Glu Lys Arg Phe370 375 380Trp Val Ala Gly Ile Ser Asn Val Leu His Arg Ser Gly Arg Glu Asn385 390 395 400Gln Arg Lys Phe Arg His Val Ser Gly Gly Ala Val Val Gly Ala Ser405 410 415Thr Arg Met Pro Gly Gly Asp Thr Leu Ser Leu Gly Phe Ala Gln Leu420 425 430Phe Ala Arg Asp Lys Asp Tyr Phe Met Asn Thr Asn Phe Ala Lys Thr435 440 445Tyr Ala Gly Ser Leu Arg Leu Gln His Asp Ala Ser Leu Tyr Ser Val450 455 460Val Ser Ile Leu Leu Gly Glu Gly Gly Leu Arg Glu Ile Leu Leu Pro465 470 475 480Tyr Val Ser Asn Thr Leu Pro Cys Ser Phe Tyr Gly Gln Leu Ser Tyr485 490 495Gly His Thr Asp His Arg Met Lys Thr Glu Ser Leu Pro Pro Pro Pro500 505 510Pro Thr Leu Ser Thr Asp His Thr Ser Trp Gly Gly Tyr Val Trp Ala515 520 525Gly Glu Leu Gly Thr Arg Val Ala Val Glu Asn Thr Ser Gly Arg Gly530 535 540Phe Phe Arg Glu Tyr Thr Pro Phe Val Lys Val Gln Ala Val Tyr Ser545 550 555 560Arg Gln Asp Ser Phe Val Glu Leu Gly Ala Ile Ser Arg Asp Phe Ser565 570 575Asp Ser His Leu Tyr Asn Leu Ala Ile Pro Leu Gly Ile Lys Leu Glu580 585 590Lys Arg Phe Ala Glu Gln Tyr Tyr His Val Val Ala Met Tyr Ser Pro595 600 605Asp Val610
權(quán)利要求
1.一種鑒定哺乳動(dòng)物如人感染肺炎衣原體的種特異性診斷測(cè)試方法，所述測(cè)試方法包括檢測(cè)患者體內(nèi)或患者樣品內(nèi)是否有針對(duì)肺炎衣原體外膜的一種或多種蛋白的抗體存在，所述蛋白的分子量為100.3-89.6kDa或56.1kDa，或檢測(cè)編碼所述外膜蛋白的核酸片段是否存在。
2.根據(jù)權(quán)利要求1所述的診斷測(cè)試方法，其中外膜蛋白具有SEQ ID NO2、SEQID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示的序列，或其變體或亞序列。
3.根據(jù)權(quán)利要求1所述的診斷測(cè)試方法，其中核酸片段具有SEQ ID NO1、SEQID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21或SEQ ID NO23所示的序列或其變體或亞序列。
4.根據(jù)權(quán)利要求3所述的診斷測(cè)試方法，其中用核酸擴(kuò)增檢測(cè)核酸片段。
5.根據(jù)權(quán)利要求4所述的診斷測(cè)試方法，其中用聚合酶鏈反應(yīng)檢測(cè)核酸片段。
6.一種衍生自肺炎衣原體的核酸片段，它包含SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21或SEQ ID NO23所示的核苷酸序列，或與任一所述序列的序列同源性至少為50％的所述核苷酸序列的變體或亞序列。
7.一種衍生自肺炎衣原體的蛋白質(zhì)，它具有SEQ ID NO2、SEQ ID NO4、SEQID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ IDNO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示的氨基酸序列，或序列同源性至少為50％且具有類(lèi)似生物功能的它的變體或亞序列。
8.一種多克隆單特異性抗體，它針對(duì)具有SEQ ID NO2、SEQ ID NO4、SEQ IDNO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示序列的蛋白或其變體或亞序列。
9.一種用來(lái)診斷哺乳動(dòng)物如人感染肺炎衣原體的診斷試劑盒，所述試劑盒包含具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示氨基酸序列的蛋白質(zhì)或其變體或亞序列。
10.一種用來(lái)診斷哺乳動(dòng)物如人感染肺炎衣原體的診斷試劑盒，所述試劑盒包含抗體，該抗體針對(duì)具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示氨基酸序列的蛋白或其變體或亞序列。
11.一種用來(lái)診斷哺乳動(dòng)物如人感染肺炎衣原體的診斷試劑盒，所述試劑盒包含具有SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21或SEQ ID NO23所示序列的核酸片段或其變體或亞序列。
12.一種用來(lái)免疫接種哺乳動(dòng)物如人來(lái)抵抗肺炎衣原體的組合物，所述組合物包含具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示氨基酸序列的蛋白質(zhì)或其變體或亞序列。
13.具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ IDNO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ IDNO20、SEQ ID NO22或SEQ ID NO24所示序列的蛋白質(zhì)或其變體或亞序列在診斷哺乳動(dòng)物如人感染肺炎衣原體中的應(yīng)用。
14.非變性形式的具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示序列的蛋白質(zhì)或其變體或亞序列在診斷哺乳動(dòng)物如人感染肺炎衣原體中的應(yīng)用。
15.具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ IDNO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ IDNO20、SEQ ID NO22或SEQ ID NO24所示序列的蛋白質(zhì)或其變體或亞序列在免疫接種哺乳動(dòng)物如人以抵抗肺炎衣原體中的應(yīng)用。
16.非變性形式的具有SEQ ID NO2、SEQ ID NO4、SEQ ID NO6、SEQ ID NO8、SEQ ID NO10、SEQ ID NO12、SEQ ID NO14、SEQ ID NO16、SEQ ID NO18、SEQ ID NO20、SEQ ID NO22或SEQ ID NO24所示序列的蛋白質(zhì)或其變體或亞序列在免疫接種哺乳動(dòng)物如人以抵抗肺炎衣原體中的應(yīng)用。
17.核酸片段或其變體或亞序列在免疫接種哺乳動(dòng)物如人以抵抗肺炎衣原體中的應(yīng)用，其中核酸片段具有SEQ ID NO1、SEQ ID NO3、SEQ ID NO5、SEQ ID NO7、SEQ ID NO9、SEQ ID NO11、SEQ ID NO13、SEQ ID NO15、SEQ ID NO17、SEQ ID NO19、SEQ ID NO21或SEQ ID NO23所示序列，所述變體或亞序列與所述任一序列的序列同源性至少為50％。
全文摘要
本發(fā)明涉及對(duì)來(lái)自人呼吸道病原體肺炎衣原體的、編碼大小約為89—101kDa和56—57kDa(較佳的約為89.6—100.3kDa和約為56.1kDa)的表面膜蛋白的基因家族成員的鑒定。本發(fā)明涉及新的DNA序列,對(duì)應(yīng)蛋白質(zhì)的推定的氨基酸序列,以及該DNA序列和蛋白在病理學(xué)和流行病學(xué)上診斷由肺炎衣原體引起的感染的用途,以及作為疫苗組分的用途。
文檔編號(hào)A61K39/395GK1261403SQ98806428
公開(kāi)日2000年7月26日 申請(qǐng)日期1998年6月19日 優(yōu)先權(quán)日1997年6月23日
發(fā)明者斯萬(wàn)德·伯克倫德, 根納·克里斯蒂安森, K·克努森, A－S·赫布斯加德·彼澤森, P·邁金迪 申請(qǐng)人:洛克診斷Aps