專利名稱：幽門螺桿菌致病島疾病基因檢測芯片的制作方法
技術(shù)領(lǐng)域：
本發(fā)明屬于臨床醫(yī)學(xué)應(yīng)用領(lǐng)域，涉及一種基因檢測芯片，進(jìn)一步地，本發(fā)明涉及幽門螺桿菌致病島疾病基因檢測芯片。
背景技術(shù)：
幽門螺桿菌(H.pylori)感染世界人口的50％以上，我國成人感染率60-70％，臨床資料表明，H.pylori的感染與消化性潰瘍、慢性胃炎、胃黏膜淋巴組織樣淋巴瘤和胃癌等疾病直接相關(guān)。與其他致病菌一樣，H.pylori的致病基因大多數(shù)與致病關(guān)系密切的基因集中在被稱為cag致病島的DNA區(qū)域。H.pylori cag致病島基因組具有較大的變異，決定了其毒力(致病能力)的差異，也是出現(xiàn)不同臨床感染結(jié)局重要因素之一。了解H.pylori cag致病島基因組多態(tài)性對于判斷疾病預(yù)后、選擇治療決策以及進(jìn)一步研究H.pylori的致病機(jī)制都有重要的意義。
目前，在分子生物學(xué)和醫(yī)學(xué)相關(guān)領(lǐng)域，H.pylori cag致病島基因組多態(tài)性研究方法主要有兩類。一類是用于分析基因結(jié)構(gòu)的分子生物學(xué)技術(shù)，涉及多聚酶鏈反應(yīng)(PCR)、DNA測序、限制性內(nèi)切酶技術(shù)和凝膠電泳等技術(shù)。原理是以目標(biāo)核酸分子為模板，通過PCR操作獲取特異性片段，再通過測序等技術(shù)了解其結(jié)構(gòu)特征。但是，現(xiàn)有分子生物學(xué)技術(shù)操作繁瑣，每一個(gè)實(shí)驗(yàn)流程只能了解一處基因信息，要完成cag致病島30個(gè)基因及超過60-70個(gè)位點(diǎn)的分析是非常龐大的工作量和實(shí)驗(yàn)周期。國內(nèi)采用此類方法的研究迄今未能獲得較為全面的H.pylori cag致病島基因致病特異性信息，因此不可能具有臨床使用價(jià)值。
另一類是采用DNA基因芯片技術(shù)研究H.pylori基因組，國外已有幾篇文獻(xiàn)報(bào)道，原理是將H.pylori所含基因的片段點(diǎn)樣至基因芯片上，有熒光標(biāo)記待測H.pylori核酸標(biāo)本與芯片雜交，再通過掃描及信息處理獲得所測H.pylori的基因組信息。但是，目前H.pylori基因組的基因芯片研究的主要目的是了解H.pylori整個(gè)基因組整體特征及完整性，未能分析與其毒力最相關(guān)的致病島基因的具體細(xì)節(jié)，不能充分提供臨床可用的信息。而且，整個(gè)基因組的芯片規(guī)模較大，費(fèi)用昂貴，不適合臨床應(yīng)用。況且，我國人群感染的H.pylori cag致病島基因組特征與國外菌株有較大差異，西方國家的資料和研究工具不適用于我國菌株的研究分析。
我國是H.pylori感染高發(fā)區(qū)，我國H.pylori菌株cag致病島完整性較高，通過我們的研究，基因結(jié)構(gòu)差異明顯、有臨床意義的cag致病島基因特征檢測工具應(yīng)該涵蓋較為具體和更加具有臨床針對性的信息。本發(fā)明是在獲得我國人群H.pylori疾病特異性菌株cag致病島全基因序列的基礎(chǔ)上，通過臨床特征相關(guān)分析，設(shè)計(jì)包含cag致病島基因及特異性基因內(nèi)部結(jié)構(gòu)細(xì)節(jié)的檢測用基因芯片?？梢暂^好地解決現(xiàn)有技術(shù)存在的不足。
此項(xiàng)發(fā)明思路和技術(shù)支持來源于三個(gè)領(lǐng)域的進(jìn)展。其一是生物工程技術(shù)中的基因芯片技術(shù)，也就是基因微矩陣技術(shù)(microarry)。原理是在玻璃、硅等載體上有序地、高密度地(目前點(diǎn)與點(diǎn)之間的距離小于500μm)排列、固定大量的靶基因片段(也稱分子探針)。樣品核酸分子經(jīng)過標(biāo)記，與固定在載體上的DNA陣列同時(shí)雜交，雜交信息通過計(jì)算機(jī)進(jìn)行集約化和平行處理，就是基因芯片技術(shù)。與傳統(tǒng)基因研究方法相比，該技術(shù)的突出特點(diǎn)是無可比擬的高信息量，高通量，快速、準(zhǔn)確地分析數(shù)以千計(jì)基因組信息，具有極高的工作效率，被譽(yù)為“芯片上的實(shí)驗(yàn)室”(Lab-on-a-chip)。其二是幽門螺桿菌分類培養(yǎng)技術(shù)。原理是體外模擬幽門螺桿菌在胃內(nèi)生長的微需氧環(huán)境和適度的營養(yǎng)環(huán)境，通過胃鏡檢查獲得H.pylori并在體外培養(yǎng)擴(kuò)增。其三是相關(guān)的分子生物學(xué)技術(shù)，包括細(xì)菌基因組DNA分離純化和熒光標(biāo)記。原理是利用化學(xué)試劑破壞菌體核酸以外的成分，再通過分離吸附等技術(shù)，獲得試驗(yàn)純度要求的基因組DNA。本發(fā)明利用現(xiàn)有技術(shù)，從解決臨床難題的角度出發(fā)，發(fā)明了一種可以一次性了解臨床病例感染H.pylori cag致病島較全面基因信息的工具。

發(fā)明內(nèi)容
本發(fā)明所要解決的技術(shù)問題在于提供一種幽門螺桿菌致病島疾病基因檢測芯片，所述的芯片上固定設(shè)置有探針，所述的探針包含SEQ ID NO1-26中85％以上保守序列的PCR產(chǎn)物，所述的PCR產(chǎn)物是以國際標(biāo)準(zhǔn)菌株HP26695序列為參考序列設(shè)計(jì)特異性引物，以臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到的。
進(jìn)一步的，本發(fā)明的目的還在于提供一種幽門螺桿菌致病島疾病基因檢測芯片，所述的芯片上還固定設(shè)置有SEQ ID NO27-40中的cag致病島基因特異性寡核苷酸。
可選擇地，本發(fā)明的幽門螺桿菌致病島疾病基因檢測芯片在制備時(shí)，所述的PCR產(chǎn)物也可以以國際標(biāo)準(zhǔn)菌株J99的序列為參考序列設(shè)計(jì)特異性引物。
進(jìn)一步的，本發(fā)明的目的還在于提供一種幽門螺桿菌致病島疾病基因檢測芯片的制作方法，包括在H.pylori感染患者中選取代表性菌株，測定其全基因組序列；與已知的標(biāo)準(zhǔn)菌株HP26695序列相比較，找出特異性的DNA多態(tài)性特征；參照保守序列設(shè)計(jì)PCR引物，將臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到產(chǎn)物作為DNA基因芯片的探針之一；對于基因內(nèi)部多態(tài)性明顯、且保守序列較短者，增加設(shè)計(jì)保守序列開放閱讀框(ORF)片段對應(yīng)的寡核苷酸片段；按照DNA基因芯片的制作工藝制備DNA基因芯片。
進(jìn)一步的，在本發(fā)明所述的幽門螺桿菌致病島疾病基因檢測芯片的制作方法中，包括下列步驟a)H.pylori感染患者中選取胃癌患者、十二指腸潰瘍患者或慢性胃炎患者，獲取代表性菌株，并測序獲得其全基因組序列；b)與已知的標(biāo)準(zhǔn)菌株HP26695序列相比較，并將不同代表菌株cag致病島基因序列相比較，尋找特異性的DNA多態(tài)性特征；c)參照保守序列設(shè)計(jì)PCR引物，將臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到產(chǎn)物作為DNA基因芯片的探針之一；對于基因內(nèi)部多態(tài)性明顯、且保守序列較短者，增加設(shè)計(jì)保守序列開放閱讀框(ORF)片段(70bp的oligoDNA，序列SEQ ID NO27-40)對應(yīng)的寡核苷酸片段，合成該片段作為額外探針；d)按照DNA基因芯片的制作工藝，將探針經(jīng)點(diǎn)樣儀點(diǎn)印在經(jīng)預(yù)處理的玻片上，經(jīng)固定工藝，制成成品DNA基因芯片。
可選擇地，在本發(fā)明所述的幽門螺桿菌致病島疾病基因檢測芯片的制作方法中，與已知的標(biāo)準(zhǔn)菌株J99的序列相比較，找出DNA序列多態(tài)性特征。
另一方面，本發(fā)明所要解決的技術(shù)問題還在于提供所述的幽門螺桿菌致病島疾病基因檢測芯片的質(zhì)量控制和檢測效率驗(yàn)證方法，其特征在于，包括下列步驟a)量待測DNA標(biāo)本(來源于待測幽門螺桿菌基因組DNA)經(jīng)隨機(jī)九聚體Cy3-dCTP或Cy5-dCTP熒光標(biāo)記原料的逆轉(zhuǎn)錄獲得不同熒光標(biāo)記的雜交樣本；b)樣本純化；c)芯片雜交實(shí)驗(yàn)；d)經(jīng)計(jì)算機(jī)熒光掃描獲取雜交信號數(shù)據(jù)；e)熒光信號數(shù)據(jù)經(jīng)標(biāo)準(zhǔn)化處理，計(jì)算Cy5/Cy3的Ratio值，所有的雜交點(diǎn)對應(yīng)的Ratio值均在0.5-2.0之間為合格。
另一方面，本發(fā)明所要解決的技術(shù)問題還在于提供所述的幽門螺桿菌致病島疾病基因檢測芯片的使用方法，包括下列步驟b)檢查獲取的幽門螺桿菌感染者胃黏膜組織標(biāo)本經(jīng)分離、培養(yǎng)48小時(shí)，得到待測菌株，并經(jīng)DNA分離純化操作獲得待測菌株的基因組DNA；c)相關(guān)上述方法獲得待測熒光標(biāo)記樣本(標(biāo)準(zhǔn)菌株DNA以Cy3熒光標(biāo)記，待測菌株DNA以Cy5標(biāo)記)；d)進(jìn)行芯片雜交實(shí)驗(yàn)，并進(jìn)行數(shù)據(jù)分析。
本發(fā)明用于檢測臨床患者(宿主)感染幽門螺桿菌菌株cag致病島疾病特異性基因型。了解這些基因型，對預(yù)后不同感染結(jié)局、制定治療和隨防策略提供指導(dǎo)。
本發(fā)明的特點(diǎn)是具有最密切的臨床聯(lián)系，我們選擇最有代表性的臨床資料在H.pylori感染患者中選取胃癌患者(H.pylori感染病史較長，考慮最有相關(guān)性)、十二指腸潰瘍患者和較輕的慢性胃炎患者(H.pylori感染病史較長，考慮H.pylori毒力較弱)，分離培養(yǎng)并單克隆化(DNA指紋分析)，獲得代表性菌株。細(xì)菌標(biāo)本來源于胃鏡檢查的胃黏膜組織標(biāo)本。如在本發(fā)明的實(shí)施例中，本發(fā)明人從臨床上獲得了161b、813f、g3e細(xì)菌。將細(xì)菌接種于Columbia瓊脂培養(yǎng)基(馬血7％，添加Skirrow選擇性抗生素)，三氣培養(yǎng)箱(N285％，CO210％，O25％)培養(yǎng)，48小時(shí)收菌。
然后，以國際標(biāo)準(zhǔn)菌株HP26695(HP26695的具體信息描述在http:∥www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl？database＝ghp)為模板，使用合適的引物設(shè)計(jì)軟件(如Primer Premier5軟件)設(shè)計(jì)PCR引物，反復(fù)PCR反應(yīng)并測序獲得上述代表菌株cag致病島的全基因組序列，獲得的PCR產(chǎn)物用常規(guī)方法純化、測序。與國外標(biāo)準(zhǔn)菌株HP26695序列相比較，并將不同代表菌株cag致病島基因序列相比較，尋找特異性的DNA多態(tài)性?；蛘撸蛇x擇地，也可以以國際標(biāo)準(zhǔn)菌株J99的序列[http:∥www.ncbi.nlm.nih.gov/entrez/viewer.fcgi？db＝nucleotide&val＝15611071，GeneBank登錄號NC_000921]為參考序列設(shè)計(jì)特異性引物。
接下來，就不同之處設(shè)計(jì)芯片點(diǎn)樣的寡核苷酸片段(對于部分內(nèi)部多態(tài)性明顯的基因增加設(shè)計(jì)保守序列開放閱讀框(ORF)片段(70bp的oligoDNA)對應(yīng)的寡核苷酸片段)，將基因?qū)?yīng)的PCR產(chǎn)物共同作為芯片點(diǎn)樣模板。待測DNA標(biāo)本的標(biāo)記采用隨機(jī)九聚體、Cy3-dCTP或Cy5-dCTP以及KlenowFragment。探針純化并芯片雜交，掃描分析。
應(yīng)理解，在通過本發(fā)明的方法獲得了特異性的cag致病島基因序列以后，本領(lǐng)域的技術(shù)人員參考本發(fā)明所提供DNA序列以及思路，可以很方便的通過各種基因工程手段制備本發(fā)明的探針，這種通過不同途徑獲得的產(chǎn)物同樣也包含在本本項(xiàng)發(fā)明可以填補(bǔ)目前國內(nèi)外專門檢測H.pylori cag致病島與臨床感染結(jié)局相關(guān)基因完整性和多態(tài)性上的空缺。與現(xiàn)有技術(shù)相比，克服了傳統(tǒng)分子生物學(xué)技術(shù)繁重的工作量和低效率，可以獲得H.pylori全基因組基因芯片分析所不能提供的具體和更具臨床應(yīng)用價(jià)值的信息。本發(fā)明的芯片規(guī)模較小、成本低、而信息量卻很大。
具體實(shí)施例方式
本發(fā)明公開了一種幽門螺桿菌致病島疾病基因檢測芯片及其制備方法，在經(jīng)修飾的載玻片上固定有探針，其中，所述探針至少包含一種與SEQ ID NO1-26序列具有85％以上同源性的序列。
由于本發(fā)明是參考國際標(biāo)準(zhǔn)菌株設(shè)計(jì)出相應(yīng)的引物，以國內(nèi)人的幽門螺桿菌菌株的為模板，獲得基因探針，制備DNA微矩陣，作為疾病的檢測的工具。國內(nèi)人的幽門螺桿菌菌株同源性非常高，在本具體實(shí)施方式
中，僅僅列舉了以本發(fā)明人在臨床上獲得的若干菌株作為對照獲得的芯片，本領(lǐng)域的人員很容易從臨床上獲得其他的菌株，獲得與本例中所列有一定差異的探針，從而可能制備出不同的芯片。因此，應(yīng)理解，這些根據(jù)本發(fā)明的方法和思路獲得的探針以及芯片包括在本發(fā)明的范圍內(nèi)。
在以下的實(shí)施例中，所述的點(diǎn)樣探針是如下獲得的，首先，在H.pylori感染患者中選取胃癌患者、十二指腸潰瘍患者和較輕的慢性胃炎患者，分離培養(yǎng)并單克隆化，獲得代表性菌株。在本實(shí)施例中，本發(fā)明利用從臨床上獲得了代表性的161b、813f、g3e細(xì)菌，將細(xì)菌接種培養(yǎng)。然后，以國際標(biāo)準(zhǔn)菌株HP26695(或J99)為模板，設(shè)計(jì)PCR引物，反復(fù)PCR反應(yīng)并測序獲得上述代表菌株cag致病島的全基因組序列，與HP26695(或J99)序列相比較，并將不同代表菌株cag致病島基因序列相比較，尋找特異性的DNA多態(tài)性。接著，參照保守序列設(shè)計(jì)PCR引物，以臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到產(chǎn)物作為DNA基因芯片的探針。
進(jìn)一步的，對于基因內(nèi)部多態(tài)性明顯、且保守序列較短者，增加設(shè)計(jì)保守序列開放閱讀框(ORF)片段對應(yīng)的寡核苷酸片段，合成所述片段作為額外探針。因此，在本發(fā)明的基因芯片上，還固定有至少一種探針，所述探針的寡核苷酸序列如表2(SEQ ID NO27-40)所示。
實(shí)施例1芯片的設(shè)計(jì)和制作1.H.pylori的培養(yǎng)及鑒定在H.pylori感染患者中選取胃癌患者、十二指腸潰瘍患者和較輕的慢性胃炎患者，分離培養(yǎng)并單克隆化，獲得代表性菌株。
將細(xì)菌接種于Columbia瓊脂培養(yǎng)基，加入7％馬血(上海生物制品所)，再添加Skirrow選擇性抗生物(5mg/l TMP，10mg/ml萬古霉素和2500U/l多粘菌素B和5mg/l兩性霉素)，在三氣培養(yǎng)箱中37℃微氧條件下(N285％，CO210％，O25％)培養(yǎng)，48h時(shí)收集菌體。經(jīng)菌落形態(tài)、涂片染色以及生化反應(yīng)(尿素酶、氧化酶和觸酶)鑒定細(xì)菌形態(tài)和活力，獲得合適的細(xì)菌。
2.、引物的設(shè)計(jì)本發(fā)明人從臨床H.pylori感染患者中獲得了161b、813f、g3e代表性菌株，采用如前所述的方法進(jìn)行培養(yǎng)，然后，提取菌株的DNA，進(jìn)行全基因組測序，獲得的161b的全基因組序列見SEQ ID NO117(161b-1，列出了其全基因組的第11-11395位核苷酸)和SEQ ID NO118(161b-2，列出了其全基因組的第13040-39189位核苷酸)；獲得的813f見SEQ ID NO119(813f-1，列出了其全基因組的第7-11146位核苷酸)和SEQ ID NO120(813f-2，列出了其全基因組的第13349-39217位核苷酸)；獲得的g3e見SEQ ID NO121(g3e-1，列出了其全基因組的第1-11329位核苷酸)和SEQ ID NO122(g3e-2，列出了其全基因組的第13155-38626位核苷酸。其中，上述菌株在測序過程中，一部分序列由于AT含量高，引物結(jié)合位點(diǎn)多，特異性差，PCR產(chǎn)物條帶多，測序無法測通，因此在有的位點(diǎn)上少了一些核苷酸，但是由于缺少的是極少量核苷酸，不影響后續(xù)的引物和探針設(shè)計(jì)。
將上述獲得的序列與已知的標(biāo)準(zhǔn)菌株HP26695的序列(參見如http:∥www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl？database＝ghp)相比較，尋找特異性的DNA多態(tài)性特征。獲得菌株基因特異性O(shè)RF和基因內(nèi)特異性變異片段，PCR操作或DNA合成得到這些片段。PCR操作引物設(shè)計(jì)的原則是長度多為18bp-22bp、具有最佳的解鏈溫度52℃-56℃和最小形成二級結(jié)構(gòu)和發(fā)卡結(jié)構(gòu)的可能性。為了保證芯片上的基因片段可以特異性地檢測相應(yīng)的基因，提交給引物設(shè)計(jì)程序的ORF序列不包括與基因組其它部分有交叉同源性的區(qū)域以及與鄰近ORF重疊的區(qū)域。同源性基因序列長度大于85％。所有引物序列通過BLAST_2sequence(來源于www.ncbi.nlm.nih.gov)與H.pylori 26695的全基因組序列進(jìn)行同源性比對，僅與基因組序列有唯一匹配位點(diǎn)的引物被采用。具體的引物選擇結(jié)果見表1所列，下表中的HP0521代表國際標(biāo)準(zhǔn)菌株HP26695的cag2基因，HP0522代表cag3基因，依次類推。由于發(fā)現(xiàn)cag1基因較短，只有300多bp，且變異明顯，因此在芯片上只使用70bp OligoDNA作為探針，未使用PCR產(chǎn)物，故這里不涉及cag1的引物。以HP編號的探針為PCR產(chǎn)物，以P編號的探針為包括多個(gè)基因長片段的PCR產(chǎn)物。獲得的正向和反向引物見表1。
表1




表2


3.模板DNA的制備以及探針的PCR擴(kuò)增反應(yīng)以前述選擇的引物作為PCR引物，以臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板(如前述臨床獲得的161b、813f、g3e)，進(jìn)行PCR反應(yīng)，得到產(chǎn)物作為DNA基因芯片的探針。其中，模板DNA的制備如下培養(yǎng)4小時(shí)后刮取細(xì)菌，于PBS中洗1-2次，每次10分鐘，采用Qiagen公司的組織DNA抽提試劑盒抽取H.pylori基因組DNA，并保存于-70℃?zhèn)溆谩?br> 接著，上述探針進(jìn)行PCR擴(kuò)增。PCR反應(yīng)總體積均為100μl。反應(yīng)體系為10×反應(yīng)緩沖液10.0μl，MgCl22.0mmol/l，dNTP 200μmol/l，引物10pmol，TaqDNA聚合酶0.6U，模板DNA 20ng。擴(kuò)增條件為94℃預(yù)變性5min，94℃ 30sec、50℃ 45sec、72℃ 1min30sec，共40個(gè)循環(huán)，終以72℃延長5min。通過1％瓊脂電泳鑒定PCR反應(yīng)是否成功，產(chǎn)物成功的標(biāo)志是條帶特異、單一、大小正確，條帶亮度適中。首次未擴(kuò)增出的基因重新設(shè)計(jì)引物進(jìn)行擴(kuò)增。
4.PCR產(chǎn)物純化和定量采用Millipore Multiscreen-PCR板純化PCR產(chǎn)物，具體純化方法參見Millipore公司提供的說明書。純化后的產(chǎn)物以雙蒸水稀釋成終濃度600ng/μl，再加以等體積的DMSO，即成50％DMSO，留待點(diǎn)片使用。選用6個(gè)與幽門螺桿菌基因組序列無同源性的人類基因和空白對照(見表3)作為陰性對照。
表3

5.點(diǎn)樣及點(diǎn)樣后的處理過程將樣品從4℃冰箱中取出，在濕度為50％-60％的環(huán)境中采用Genemachine點(diǎn)樣儀進(jìn)行點(diǎn)樣。除了將上述制備獲得的探針點(diǎn)樣在芯片上；還點(diǎn)樣了表2所列的寡核苷酸探針。每個(gè)基因片段重復(fù)點(diǎn)樣4次。點(diǎn)樣完后在400mJ能量下采用交聯(lián)儀CL-1000 Ultraviolet Crosslinker進(jìn)行交聯(lián)。置芯片于室溫干燥24h。抽查并以掃描儀觀察芯片上各個(gè)點(diǎn)的形狀和強(qiáng)度。
實(shí)施例2.本發(fā)明的芯片與待測DNA標(biāo)本的雜交以下雜交過程中所用到的試劑均可商購，來源于上海博星基因芯片公司，如標(biāo)III、DNA雜交液I、DNA雜交液II等。
1.在50ul反應(yīng)體系中待測基因的標(biāo)記過程(1)將樣本DNA 3μg溶解在水中，制成25μl的溶液。取1μg/μl的隨機(jī)引物6μl，于95℃變性5分鐘，然后在冰上放置2分鐘。
(2)在暗室，在上述溶液中加入5ul的10×Buffer、2ul的dNTP、1ul的1mMCy5-dCTP、1ul的Klenow酶(50u/ul)，將混合物在37℃水浴2小時(shí)，接著在95℃水浴2分鐘。然后合并Cy5、Cy3，過柱純化并質(zhì)檢。
(3)加標(biāo)III 8ul，抽干。
(4)加入DNA雜交液I(除去魚精DNA)6.5ul和雜交液II 6.5ul，在95℃變性2分鐘，在42℃雜交16小時(shí)。
(5)在50℃，洗滌芯片。
2.芯片的預(yù)雜交(1)將配制好的預(yù)雜交液置于95℃，變性30秒；用無水乙醇處理30秒。
(2)加入13ul DNA雜交液I(除去魚精DNA)，在95℃變性2分鐘。
(3)在42℃雜交6小時(shí)；然后雙蒸水沖洗芯片。
(4)將芯片置于95℃，變性30秒；用無水乙醇處理30秒；用于標(biāo)記DNA雜交。
3.雜交反應(yīng)采用隨機(jī)九聚體、Cy3-dCTP/Cy5-dCTP以及Klenow Fragment進(jìn)行基因組DNA標(biāo)記，分別用Cy3和Cy5標(biāo)記參照標(biāo)本[熒光標(biāo)記的26695菌株的DNA]和試驗(yàn)標(biāo)本[熒光標(biāo)記的待測菌株的DNA]。步驟1).超聲波粉碎的基因組DNA樣品。2).取2ug粉碎后的基因組DNA樣品，加入8ul 2.5×隨機(jī)引物/反應(yīng)緩沖液，于100℃變性5min，然后冰上放置5min。3).加入2ul 10×dNTP混合物、1.2ulCy5-dCTP或Cy3-dCTP(Amersham，1mM stocks)、0.5ul klenow Fragment，反應(yīng)混合物在37℃保溫3小時(shí)。4).在PCR管中加入2μl 0.5M EDTA(pH8.0)以終止反應(yīng)。5)熒光探針的純化和雜交過程。未吸收的熒光染料采用QIAquick NucleotideRemoval Kit(Qiagen，Germany)進(jìn)行去除。步驟在標(biāo)記反應(yīng)結(jié)束后，加入10倍體積的緩沖液PN，6000rpm，離心30sec。加入750ul緩沖液PE，12000rpm，離心1min。
換用新的離心管，12000rpm，離心1min。終以120ul EB洗脫探針。探針轉(zhuǎn)入酶標(biāo)板進(jìn)行定量，分別測定550nm和650nm波長處的吸光值。各取出30pmolCy3和Cy5標(biāo)記的探針進(jìn)行雜交。在Speed Vac抽干儀中進(jìn)行探針抽干。將探針重懸于9μl ddH2O中，95℃變性5min。加入11μg鮭精DNA，在45℃孵育45min。加入10μl 4×Buffer和20μl甲酰胺，將混合物平鋪于芯片上。將芯片水平放入加有PBS的雜交盒或者濕盒，置65℃雜交箱中避光雜交6小時(shí)。將芯片取出，分別在50℃洗液I和洗液II中洗10min，各2次。最終在室溫下洗液III中洗5min，2次。1500rpm離心5min，避光待掃描。
4.數(shù)據(jù)的讀取、處理與分析芯片的掃描采用Agilent掃描儀，讀圖軟件為Imagene 5.1。芯片數(shù)據(jù)的標(biāo)化方法采用Standford Microarray Database中的缺省值標(biāo)化方法。由標(biāo)準(zhǔn)化后的數(shù)據(jù)取得Cy3/Cy5的ratio值。ratio值的cut-off值設(shè)定為0.5，小于0.5者認(rèn)為是陰性，大于或等于0.5為陽性，數(shù)據(jù)分析時(shí)分別以0和1表示。用于研究用的大樣本檢測還可以進(jìn)行聚類分析。
表3顯示了用該芯片檢測27例臨床幽門螺桿菌得到的結(jié)果，其中，第一行表示臨床菌株，第一列表示基因芯片的探針相關(guān)的基因信息。
表3








<110>上海第二醫(yī)科大學(xué)附屬仁濟(jì)醫(yī)院蕭樹東，陳勝良<120>幽門螺桿菌致病島疾病基因檢測芯片<160>34<170>PATENTIN VERSION 3.1<210>1<211>345<212>DNA<213>HELICOBACTER PYLORI<220>
<221>GENE<222>(1)..(348)<223>
<400>1ATGGCTGACA CAATCAATAC AACTGAAGCA ACTCATGAAA CAAAAAAACC AAACGCTTTT60GTAAATTTTT TCAAAAACAA TTTGACTGAT AAGCGTTATG ATTCATTAGG TCTCATTGGA120GCAGGGGTTT TATGTTGTGT CTTGAGCGGT GCTATGGGGA TTGTTGGGAT AATCTTTGTC180GCAATAGGAA TCTTTTTGTC TTTTTCTAAT ATCAACTTAG TGAAATTAGT TGAAAAATTG240TCCAAAAAAC AATCTAAAGT GCCAACAACT GTCAATAACG AAACTCAAAA ATCTCAAGCA300ACAAGCGTTA CCAACGAACC AACTGAAGCC AAAGAGACTA AAGATTGA 348<210>2<211>243<212>DNA<213>HELICOBACTER PYLORI<400>2ATGATACAAA GAGGATTGAG TAGTGTCTCA TTTTATTATG GTTGCTCTAA TTATGTAAAT60AAAGGCGGCT GTAAGGGCGT TTTACGAGAA ATAAATGGCT CAATGAAAAT GGTTTGCTTG120GCTAGTATAA AAGCTAATAT TAAAGAAAAT TCTTTTTTCT TGGGGTGTAA AATTATCTTA180AGTGTAAATG GACTGCTAGT GTGGATTCAC AAAATCCTAA ATATCCCAAA TGCAATGGAT240
TGA 243<210>3<211>1446<212>DNA<213>HELICOBACTER PYLORI<400>3ATGTTTAGAA AACTAGCAAC CGCTGTATCG CTCATAGGCT TACTAACCTC TAACACTCTT60TATGCTAAAG AAATAAGTGA AGCCGATAAG GTCATTAAGG CCACTAAAGA AACTAAAGAG120ACCAAGAAAG AAGCTAAACG ACTCAAAAAA GAAGCTAAAC AGCGCCAACA GATCCCTGAT180CATAAGAAAC CTCAATATGT CTCTGTTGAT GACACAAAAA CTCAAGCGCT GTTTGATATA240TACGACACCT TGAATGTGAA TGACAAAAGC TTTGGGGATT GGTTTGGTAA TAGCGCTTTG300AAAGACAAAA CCTATCTCTA CGCTATGGAT CTATTGGATT ACAACAACTA TTTATCCATA360GAAAACCCCA TTATCAAAAC AAGAGCAATG GGAACTTATG CGGATTTGAT TATCATCACA420GGCTCATTAG AGCAAGTCAA TGGGTATTAC AACATTCTAA AAGCGCTCAA CAAACGCAAC480GCTAAGTTTG TGTTAAAAAT CAATGAGAAC ATGCCTTATG CCCAAGCGAC TTTTTTAAGA540GTGCCAAAAA GAAGCGATCC TAATGCCCAC ACGCTTGATA AGGGAGCGTC AATTGATGAG600AATAAGCTTT TTGAACAACA AAAGAAAATG TATTTCAATT ACGCTAACGA TGTGATCTGC660AGACCCGATG ATGAAGTGTG TTCGCCCCTA AGAGATGAGA TGGTAGCTAT GCCCACTAGC720GATAGCGTTA CTCAAAAACC CAATATCATT GCTCCTTATA GCTTGTATAG ACTAAAAGAG780ACAAATAACG CCAATGAGGC CCAACCATCA CCTTATGCCA CTCAAACCGC TCCCGAAAAC840AGCAAAGAGA AGCTCATAGA AGAGCTAATC GCTAACTCCC AACTCGTAGC CAATGAAGAA900GAGAGGGAAA AGAAACTCTT AGCAGAAAAA GAAAAACAAG AGGCTGAATT GGCTAAATAT960AAGCTCAAAG ACTTAGAAAA TCAAAAGAAA CTAAAAGCTT TAGAAGCAGA GTTGAAAAAG1020AAAAACGCTA AGAAACCTAG AGTAGTGGAA GTGCCTGTTT CTCCTCAAAC AAGTAATTCT1080GATGAAACAA TGAGAGTTGT CAAAGAAAAA GAGAACTATA ATGGGTTATT AGTGGATAAG1140GAGACCACGA TCAAAAGAAG CTATGAGGGG ACTTTGATCA GTGAAAATTC TTACAGCAAA1200AAAACACCTC TCAACCCTAA TGACTTGAGG AGCTTAGAAG AAGAAATTAA GAGCTACTAT1260ATCAAGTCTA ATGGCTTGTG TTATACTAAT GGCATTAACC TCTATGTAAA AATCAAAAAC1320GACCCCTATA AAGAGGGAAT GCTGTGTGGT TATGAGAGCG TTCAAAATCT GCTCTCACCT1380
CTAAAGGACA AGCTCAAATA CGACAAACAA AAGTTACAAA AAGCGTTACT GAAAGATTCA 1440AAGTAA 1446<210>4<211>510<212>DNA<213>HELICOBACTER PYLORI<400>4TTGTTCGAGA AATGGATTGG TCTGACTTTG CTTCTTAATT CCTTAGCCTA TCCATGCCAA60AAGGTAACCA TTAGTTTCAA GCAGTATGAA AATCTTCTCC ATATCCATCA AAAAGGTTGC120GACAATGAAG TGATGTGCAG AACGCTCATC TCTATCGCTT TGTTAGAAAG CTCTCTAGGG180TTGAACAACA GGCGAGAAAA ATCCCTTAAA GACACTTCTT ATTCCATGTT TCATATCACC240CTAAACACCG CTAAAAAATT CTACCCTACC TACTCTAAAA CGCTCCTCAA ATTCAAATTG300CTAAACGATG TGGGTTTTGC GATCCAATTA GCCAAACAAA TTTTAAAAGA AAATTTTGAT360TATTACAAAC AAAAACACCC CAACAAAAGC GTGTATCAAT TAGTAGAAAT GGCAATAGGC420GCTTACAATG GGGGAATGAA ACACAACCCT AATGGCGCTT ACGTGAAAAA ATTCCGTTGC480ATTTATTCTC AAGTGCGATA TAACGAGTAG 510<210>5<211>2247<212>DNA<213>HELICOBACTER PYLORI<400>5ATGGAAGACT TTTTGTATAA CACCTTATAT TTCATAGAGG ATTATAAGTT GGTTGTTATT60TTTAGTTTCA TAGGGTTAAT AGCGTTATTT TTTCTTTACA AATTCATAAA AGCTCAAAAA120AAGGCTTTTA AAGATAAAGC TAACCAGCCT CAAAAGAAAA AAAGCTTTAA AGAAATCATT180ATAGATGGGC TGAAAGAAAG GGTTAAAACC TTTGGCTTTT GGTTGCAAGC TATACTATTA240CTATCCTATT CTTTTATCAC ATCAGGATTA TTTTTCTTGA TTCTCTTAGG TAATTTTTAT300GATGATAATC GATCGCCTGA GAGCGATGAT GATCTTTTTG ATATATGGAT CTATGCGATA360CAAGATTTTC CTAATTACTA TTTTAAAGCG CTTGGTTTTA GTTCACTCAA GATTTATGGG420TTCAATATAT CCTTAGTCGT ATATGGTTCT ATTTTATGCT CTTATATCTT CATTACCTTT480
TTTGTGTGGT TCTTAAAATA CTTAACTCGG ACTAGAGATA TAGGAGCGAA TAAAAAAGTT540GATGATCTCT TTGGTAGCGC GAGTTGGGAA ACTGAAGAGA AAATGATCAA AGCCAAACTC600ATCACGCCCA ACAATAAAAA ACGCGCCTTT GACAAACGAG AGGTGATTGT AGGCAGGCGT660GGCTTGGGGG ATTTTATCGC TTACGCAGGG CAGGCGTTCA TTGGCTTGAT TGCTCCTACT720AGAAGCGGTA AGGGGGTGGG TTTCATCATG CCCAATATGA TCAATTATCC TCAAAATATC780GTTGTGTTTG ACCCTAAAGC TGACACTATG GAGACTTGCG GAAAAATCAG AGAAAAACGC840TTCAACCAAA AAGTGTTCAT CTATGAACCT TTCTCCTTAA AAACACACCG ATTTAATCCT900TTCGCTTATG TGGATTTTGG TAATGATGTG GTTTTGACCG AAGACATACT CTCTCAAATT960GACACACGCC TAAAAGGGCA TGGCATGGTG GCTAGTGGAG GGGATTTTTC CACTCAAATC1020TTTGGATTAG CAAAGCTCGT GTTCCCTGAA AGACCTAATG AAAAAGATCC TTTCTTTAGC1080AATCAAGCGC GAAATCTTTT TGTCATCAAT TGCAATATTT ACAGGGATCT CATGTGGACT1140AAAAAGGGGC TTGAGTTTGT CAAAAGAAAA AAAATCATCA TGCCTGAAAC ACCCACGATG1200TTTTTCATAG GTTCTATGGC AAGCGGGATC AACTTGATTG ATGAAGACAC AAACATGGAA1260AAAGTCGTGT CTTTAATGGA ATTTTTTGGA GGTGAAGAAG ATAAGAGTGG CGATAATCTA1320AGAGTGCTTA GTCCTGCCAC TAGAAACATG TGGAATAGCT TCAAGACAAT GGGCGGCGCT1380AGAGAAACTT ATAGCTCGGT TCAAGGGGTA TACACTTCAG CCTTTGCGCC TTATAATAAC1440GCAATGATTA GAAATTTCAC GAGCGCCAAT GATTTTGATT TCAGGCGTTT AAGGATCGAT1500GAAGTGAGTA TTGGTGTGAT CGCTAATCCT AAAGAAAGCA CTATTGTTGG ACCGATATTA1560GAGCTGTTTT TCAATGTGAT GATTTATAGC AATTTGATTC TGCCAATCCA TGATCCACAG1620TGCAAAAGAA GTTGCTTGAT GCTCATGGAC GAATTCACTT TATGTGGCTA TTTAGAGACC1680TTTGTTAAAG CGGTAGGGAT TATGGCAGAA TACAACATGC GCCCCGCTTT TGTGTTTCAA1740AGTAAGGCGC AACTAGAGAA TGACCCCCCA CTTGGTTATG GTAGGAATGG CGCTAAGACT1800ATTTTAGACA ACCTTTCTTT GAATATGTAT TATGGGATTA ACAACGATAA CTACTATGAA1860CATTTTGAAA AACTTTCTAA GGTATTAGGG AAATACACAA GGCAAGACGT GAGCCGAAGC1920ATTGATGATA ATACAGGTAA GACCAACACT TCTATCAGCA ACAAAGAGCG GTTTTTGATG1980ACCCCTGATG AATTGATGAC TATGGGCGAT GAGCTTATCA TTCTAGAGAA TACGCTCAAA2040CCTATCAAGT GCCACAAGGC GCTTTACTAT GATGATCCAT TCTTCACCGA TGAACTCATT2100AAGGTAAGTC CAAGCTTGAG CAAGAAATAC AAATTGGGGA AAGTGCCTAA TCAAGCAACT2160TTCTATGATG ATTTGCAAGC CGCTAAAACT AGAGGTGAAT TGAGTTATGA TAAATCTTTA2220GTGCCTGTGG GTTCAAGTGA ACTGTGA2247
<210>6<211>600<212>DNA<213>HELICOBACTER PYLORI<400>6ATGGAACTCG GTTTCAATGA AGCAGAAAGA CAAAAGATCT TAGATAGCAA CAGCTCTCTT60ATGGGAAATG CAAATGAAGT AAGGGATAAG TTTATTCAAA ATTACGCCTC TTCTTTAAAA120GATAGCAACG ATCCGCAAGA TTTTTTGAGA AGAGTTCAAG AGTTAAGAAT CAATATGCAA180AAGAATTTTA TTAGTTTTGA TGTTTATTAC AACTATTTGA ACAACCTTGT GTTGGCCAGT240TACAATCGTT GCAAACAAGA AAAGACTTTT GCAGAAAGCA CGATCAAAAA TGAACTAACG300CTTGGGGAGT TTGTTGCAGA AATTTCTGAC AACTTCAATA ATTTCATGTG TGATGAAGTG360GCAAGAATTT CAGACCTAGT GGCTTCTTAT CTGCCAAGAG AGTATTTACC GCCATTCATA420GATGGCAATA TGATGGGCGT GGCGTTTCAG ATCCTAGGGA TAGATGATTT TGGAAGGAAG480CTCAATGAGA TTGTCCAAGA TATAGGGACT AAATATATTA TTTTGAGCAA AAATAAGACT540TATCTCACTT CTTTAGAAAG AGCTAAATTG ATAACCCAAT TAAAATTAAA TTTGGAATAA600<210>7<211>5784<212>DNA<213>HELICOBACTER PYLORI<400>7ATGAATGAAG AAAACGATAA ACTTGAAACT TCTAAAAAAG CCCAACAAGA TTCACCCCAA60GATTTATCCA ATGAAGAAGC AACAGAAGCC AATCATTTTG AAAATCTTTT AAAAGAATCC120AAAGAAAGCT CAGATCATCA TCTTGACAAC CCCACAGAAA CTCAAACCCA TTTTGATGGA180GACAAGTCAG AAGAAACCCA AACTCAAATG GATTCTGAAG GTAATGAAAC TTCAGAATCT240AGCAATGGCA GTCTAGCAGA CAAGTTATTC AAAAAAGCCA GAAAATTAGT TGATAATAAA300AAACCTTTCA CTCAGCAAAA GAATTTAGAT GAAGAAACCC AAGAACTGAA CGAAGAAGAC360GATCAAGAAA ATAATGAGTA TCAAGAAGAA ACTCAAACGG ACTTAATTGA TGATGAAACT420TCTAAAAAAA CCCAACAACA TTCACCCCAA GATTTATCCA ATGAAGAAGC AACAGAAGCC480AATCATTTTG AAAATCTTTT AAAAGAATCC AAAGAAAGCT CAGATCATCA TCTTGACAAC540
CCCACAGAAA CTCAAACCAA TTTTGATGGA GACAAGTCAG AAGAAACCCA AACTCAAATG600GATTCTGAAG GTAATGAAAC TTCAGAATCT AGCAATGGCA GTCTAGCAGA CAAGTTATTC660AAAAAAGCCA GAAAATTAGT TGATAATAAA AAACCTTTCA CTCAGCAAAA GAATTTAGAT720GAAGAAACCC AAGAACTGAA CGAAGAAGAC GATCAAGAAA ATAATGAGTA TCAAGAAGAA780ACTCAAACGG ACTTAATTGA TGATGAAACT TCTAAAAAAA CCCAACAACA TTCACCCCAA840GATTTATCCA ATGAAGAAGC AACAGAAGCC AATCATTTTG AAAATCTTTT AAAAGAATCC900AAAGAAAGCT CAGATCATCA TCTTGACAAC CCCACAGAAA CTCAAACCAA TTTTGATGGA960GACAAGTCAG AAGAAATAAC TGACGACTCT AACGATCAAG AGATTATCAA AGGAAGCAAA1020AAGAAATATA TTATTGGTGG CATTGTAGTC GCTGTTCTTA TCGTGATTAT TTTATTTTCT1080AGAAGCATTT TTCACTACTT CATGCCTTTG GAAGATAAAA GCTCTCGTTT TAGCAAAGAC1140AGGAATCTTT ATGTCAATGA TGAAATCCAA ATAAGGCAAG AGTATAACCG ATTGCTGAAA1200GAACGGAATG AAAAAGGCAA TATGATCGAT AAGAATCTTT TCTTCAATGA CGATCCCAAT1260AGAACCTTAT ACAACTATTT GAATATTGCA GAAATTGAGG ACAAAAACCC GTTGAGAGCC1320TTTTATGAAT GTATTAGTAA TGGTGGCAAC TATGAAGAAT GTTTGAAGCT TATCAAAGAC1380AAAAAACTTC AAGATCAGAT GAAAAAGACT CTAGAGGCTT ATAACGACTG CATCAAAAAT1440GCCAAAACTG AAGAAGAAAG GATCAAGTGT TTAGATTTAA TCAAAGATGA AAACCTAAAA1500AAAAGCTTAC TGAACCAACA AAAAGTTCAA GTGGCGCTAG ATTGTTTGAA AAACGCTAAA1560ACCGATGAAG AACGAAACGA GTGCCTAAAA CTCATAAATG ACCCTGAGAT TAGAGAGAAA1620TTCCGTAAGG AATTAGAGCT TCAAAAAGAG CTTCAAGAGT ATAAGGATTG TATCAAAAAC1680GCCAAAACAG AAGCTGAGAA AAACAAATGC TTGAAAGGCT TGTCTAAAGA AGCTATAGAG1740AGATTGAAAC AGCAAGCGCT AGATTGTTTG AAAAACGCTA AAACCGATGA AGAACGAAAC1800GAGTGCTTGA AAAATATTCC CCAAGACTTG CAAAAAGAAC TATTAGCTGA TATGAGCGTC1860AAGGCTTACA AGGATTGCGT ATCAAAAGCT AGAAATGAAA AAGAGAAACA AGAATGCGAG1920AAATTGCTCA CGCCTGAAGC GAGGAAAAAG TTAGAACAAC AGGTTCTAGA TTGTTTGAAA1980AACGCTAAAA CCGATGAAGA ACGAAAAAAG TGTTTGAAAG ATCTCCCTAA AGACTTACAA2040AGCGATATTC TAGCCAAAGA GAGCCTGAAA GCTTATAAAG ACTGCGTATC TCAAGCCAAA2100ACCGAAGCTG AGAAAAAAGA ATGCGAGAAA TTACTCACCC CTGAAGCGAA AAAACTTTTA2160GAAGAAGAAG CCAAAGAGAG CGTTAAGGCT TATTTGGATT GCGTATCTCA AGCCAAAACC2220GAAGCTGAGA AAAAAGAATG CGAGAAATTG CTCACCCCTG AAGCGAAAAA AAAGTTAGAA2280GAAGCTAAAA AAAGCGTTAA AGCTTACTTG GATTGCGTAT CAAGAGCTAG GAATGAAAAA2340GAGAAAAAAG AATGCGAGAA ATTGCTCACC CCTGAAGCGA AAAAACTTTT AGAGCAACAA2400
GCACTAGATT GTTTGAAAAA CGCTAAAACC GATAAAGAAC GAAAAAAGTG TTTGAAAGAT2460CTCCCTAAAG ACTTGCAGAA AAAGGTTTTA GCTAAAGAAA GCGTTAAAGC TTACTTGGAT2520TGCGTATCTC AAGCCAAAAC TGAAGCTGAG AAAAAAGAAT GCGAGAAATT ACTCACCCCT2580GAAGCGAGAA AACTTTTAGA AGAAGCTAAA AAAAGCGTTA AGGCTTATTT GGATTGCGTA2640TCTCAAGCCA AAACTGAAGC TGAGAAAAAA GAATGCGAGA AATTACTCAC CCCTGAAGCG2700AGAAAACTTT TAGAAGAAGA MGCCAAAGAG AGCGTTAAAG CTTACTTGGA TTGCGTATCT2760CAAGCCAAAA ACGAAGCTGA GAAAAAAGAA TGCGAGAAAT TGCTCACCCT TGAATCGAAA2820AAAAAGTTAG AAGAAGCTAA AAAAAGCGTT AAGGCTTATT TGGATTGCGT ATCTCAAGCC2880AAAACCGAAG CTGAGAAAAA AGAATGCGAA AAATTGCTCA CGCCTGAAGC GAAAAAACTT2940TTAGAGCAAC AAGCGCTAGA TTGTTTGAAA AACGCTAAAA CCGAAGCTGA TAAAAAAAGG3000TGTGTCAAAG ATCTCCCTAA AGACTTGCAG AAAAAGGTTT TAGCCAAAGA GAGCCTGAAA3060GCTTATAAAG ACTGCGTATC AAAAGCTAGG AATGAAAAAG AGAAAAAAGA ATGCGAGAAA3120TTACTCACCC CTGAAGCGAA AAAACTTTTA GAAGAAGCTA AAAAAAGCGT TAAGGCTTAC3180TTGGATTGCG TATCTCAAGC CAAAACTGAA GCTGAGAAAA AAGAATGCGA GAAATTACTC3240ACCCCTGAAG CGAGAAAACT CTTAGAAGAA GCTAAAGAGA GCGTTAAAGC TTATAAAGAC3300TGCGTATCAA AAGCTAGGAA TGAAAAAGAG AAAAAAGAAT GCGAGAAATT ACTCACGCCT3360GAAGCGAAAA AACTTTTAGA GCAACAAGTG CTAGATTGTT TGAAAAACGC TAAAACCGAA3420GCTGATAAAA AAAGGTGTGT CAAAGATCTC CCTAAAGACT TGCAGAAAAA GGTTTTAGCT3480AAAGAGAGCG TTAAGGCTTA TTTGGACTGC GTATCAAGAG CTAGGAATGA AAAAGAGAAA3540AAAGAATGCG AGAAATTGCT CACCCCTGAA GCGAAAAAAC TTTTAGAAGA AGCCAAAGAG3600AGTCTTAAAG CTTATAAAGA CTGCCTCTCT CAAGCTAGAA ATGAAGAAGA AAGGAGAGCT3660TGCGAGAAAC TACTCACGCC TGAAGCGAGA AAACTCTTAG AGCAAGAAGT TAAGAAAAGC3720ATTAAGGCTT ATTTGGACTG CGTATCAAGA GCTAGGAATG AAAAAGAGAA AAAAGAATGC3780GAGAAATTAC TCACGCCTGA AGCGAGAAAA TTTTTAGCGA AGCAAGTGCT AAATTGTTTG3840GAAAAAGCTG GAAATGAAGA AGAAAGAAAA GCATGTCTTA AAAATCTCCC TAAAGACTTA3900CAGGAAAATA TTTTAGCTAA AGAGAGTCTT AAAGCTTATA AAGACTGCCT CTCTCAAGCT3960AGAAATGAAG AAGAAAGGAG AGCTTGCGAG AAACTACTCA CGCCTGAAGC GAGAAAACTC4020TTAGAGCAAG AAGTTAAGAA AAGCGTTAAG GCTTATTTGG ACTGCGTATC AAGAGCTAGG4080AATGAAAAAG AGAAAAAAGA ATGCGAGAAA TTACTCACGC CTGAAGCGAG AAAATTTTTA4140GCGAAAGAAC TCCAACAAAA AGATAAAGCG ATCAAAGATT GCTTGAAAAA CGCCGATCCT4200AACGACAGAG CGGCTATCAT GAAGTGTTTG GATGGTTTGA GCGATGAAGA GAAGCTCAAA4260
TACCTGCAAG AAGCTAGAGA AAAGGCTGTT GCGGATTGTT TGGCTATGGC TAAAACCGAT4320GAAGAAAAAA GGAAATGCCA AAACCTTTAT AGCGATTTGA TCCAAGAAAT CCAAAATAAA4380AGGACACAAA ACAAACAAAA TCAATTGAGT AAAACAGAAA GGTTGCATCA AGCAAGCGAG4440TGCTTGGATA ACTTAGATGA CCCTACTGAT CAAGAGGCCA TAGAGCAATG TTTAGAGGGC4500TTGAGCGATA GTGAAAGGGC GCTAATTCTA GGAATTAAAC GACAAGCTGA TGAAGTGGAT4560CTGATTTATA GCGATCTAAG AAACCGTAAA ACCTTTGATA ACATGGCGGC TAAAGGTTAT4620CCATTGTTAC CAATGGATTT CAAAAATGGC GGCGATATTG CCACTATTAA CGCCACTAAT4680GTTGATGCGG ACAAAATAGC TAGCGATAAT CCTATTTATG CTTCCATAGA GCCTGATATT4740GCCAAGCAAT ACGAAACAGA AAAAACCATT AAGGATAAGA ATTTAGAAGC TAAATTAGCT4800AAGGCTTTAG GTGGCAATAA AAAAGATGAC GATAAAGAAA AAAGTAAAAA ATCCACAGCA4860GAAGCTAAAG CAGAAAACAA TAAGATAGAC AAAGATGTCG CAGAAACTGC CAAGAATATC4920AGTGAAATCG CTCTTAAGAA CAAAAAAGAA AAGAGTGGGG AATTTGTAGA TGAAAATGGT4980AATCCCATTG ATGACAAAAA GAAAGCAGAA AAACAAGATG AAACAAGCCC TGTCAAACAG5040GCCTTTATAG GCAAGAGTGA TCCCACATTT GTTTTAGCGC AATACACCCC CATTGAAATC5100ACTCTGACTT CTAAAGTAGA TGCCACTCTC ACAGGTATAG TGAGTGGGGT TGTAGCCAAA5160GATGTATGGA ACATGAACGG CACTATGATC TTATTAGACA AAGGCACTAA GGTGTATGGG5220AATTATCAAA GCGTGAAAGG TGGCACACCC ATTATGACAC GCTTAATGAT AGTCTTTACT5280AAAGCCATTA CGCCTGATGG TGTGATAATA CCTCTAGCAA ACGCTCAAGC AGCAGGCATG5340TTGGGTGAAG CAGGGGTAGA TGGCTATGTG AATAATCACT TTATGAAGCG CATAGGCTTT5400GCTGTGATAG CAAGCGTGGT TAATAGCTTC TTGCAAACTG CGCCTATCAT AGCTCTAGAT5460AAACTCATAG GCCTTGGCAA AGGTAGAAGT GAAAGGACAC CTGAATTTAA TTACGCTTTG5520GGTCAAGCTA TCAATGGTAG CATGCAAAGT TCAGCTCAGA TGTCTAATCA AATTCTAGGG5580CAACTGATGA ATATCCCCCC AAGTTTTTAC AAAAACGAGG GCGATAGTAT TAAGATTCTC5640ACAATGGACG ATATTGATTT TAGCGGTGTG TATGATGTTA AAATTACTAA CAAATCTGTG5700GTAGATGAAA TTATCAAACA AAGCACCAAA ACTTTGTCTA GAGAACATGA AGAAATCACC5760ACAAGCCCCA AAGGTGGCAA TTAA 5784<210>8<211>1569<212>DNA
<213>HELICOBACTER PYLORI<400>8ATGGGGCAGG CATTTTTTAA AAAAATTGTT GGCTGTTTCT GTCTTGGTTA TTTATTTTTA60TCTAGCGCAA TAGAAGCAGC AGCACTTGAC ATTAAGAATT TTAATCGTGG TAGGGTGAAA120GTGGTGAATA AGAAGATTGC TTATTTGGGA GATGAAAAAC CTATTACGAT TTGGACTTCA180TTAGACAATG TTACCGTGAT CCAACTTGAA AAAGATGAAA CTATTTCTTA CATCACAACA240GGTTTCAATA AAGGTTGGAG TATTGTGCCT AATTCTAATC ATATATTCAT TCAACCTAAA300TCGGTAAAAA GTAATCTCAT GTTTGAAAAA GAAGCAGTGA ATTTTGCCCT AATGACAAGA360GATTACCAAG AATTTTTAAA AACAAAAAAA CTTATCGTAG ATGCGCCTGA CCCTAAAGAA420TTAGAAGAAC AAAAAAAAGC TCTAGAAAAA GAAAAAGAAG CTAAAGAACA AGCGCAAAAA480GCACAAAAAG ATAAAAGAGA AAAAAGAAAA GAAGAGCGTG CAAAAAATAG AGCCAATTTA540GAAAATCTCA CTAACGCTAT GAGTAACCCA CAAAATTTGA GCAATAACAA AAATCTTAGC600GAATTTATCA AGCAACAACG AGAAAATGAA TTAGACCAAA TGGAACGACT AGAGGACATG660CAAGAACAGG CTCAAGCTAA TGCGCTCAAA CAAATTGAAG AACTCAACAA GAAACAAGCT720GAAGAGACAA TCAAGCAAAG AGCCAAAGAT AAAATCAATA TTAAGACAGA TAAGCCTCAA780AAAAGCCCTG AGGATAACTC CATAGAATTA TCTCCTAGCG ATAGCGCTTG GAGAACTAAT840CTTGTTGTGC GGACTAATAA AGCCTTGTAT CAATTCATTT TGAGAATAGC TCAAAAAGAC900AATTTTGCTT CAGCGTATCT AACAGTCAAA TTAGAATACC CACAAAGACA CGAAGTCTCT960AGCGTTATTG AAGAGGAGTT AAAAAAGAGA GAAGAAGCAA AGAGGCAGAA AGAATTGATT1020AAGCAAGAAA ATCTTAACAC CACAGCCTAC ATCAATAGAG TGATGATGGC GAGCAATGAA1080CAGATTATCA ACAAAGAAAA AATAAGAGAA GAAAAGCAAA AAATTATCTT AGATCAAGCA1140AAGGCGCTAG AGACTCAATA TGTGCATAAT GCCTTAAAAA GAAACCCCGT GCCTAGAAAC1200TACAACTACT ACCAAGCGCC TGAAAAACGC TCTAAACATA TTATGCCCTC TGAAATTTTT1260GATGATGGCA CATTCACTTA TTTTGGTTTC AAAAACATCA CTCTCCAACC TGCTATTTTT1320GTGGTTCAAC CTGATGGGAA ATTGAGCATG ACTGATGCCG CCATTGATCC TAACATGACC1380AATTCAGGAT TGAGATGGTA TAGAGTTAAT GAAATTGCAG AAAAATTTAA GCTCATTAAA1440GACAAAGCCC TTGTAACAGT AATCAATAAA GGCTATGGGA AAAATCCATT GACAAAAAAT1500TACAATATCA AAAACTATGG TGAATTGGAG CGTGTGATTA AAAAGCTCCC TCTTGTCAGA1560GATAAATAA1569<210>9
<211>1608<212>DNA<213>HELICOBACTER PYLORI<400>9ATGTTTAATA TTAAAAGGAA TTTTTTAATA ACGATCATAA GTTTTTTTCT CATTGTTCCT60AATTGGTTGA AAGCTATTGA TTTGCCCATT GTTTCAAATC TCAAAATTTA CCAAACAGTT120TATTGCATGC TGATACCGAG TTATGTTTTA ACCAACAAAA GTTTTGCAGA TATTTTAACA180GGCTATACAT CTATTGGTGC ATCAGGGAGT GGAAAGAGTT CAGGGCAGGG TGTGATTGAA240GCGCTTAGCA CACCATTAGC CACAAGTTTA GCCGCTAGCA ATCTGGTGAA ATATTTGAAT300ACTTTAGGTC CTTTATGGGG ATCGGCGTGG GCAAGTGTTG CTACAGCTAT ACAAGGTTTT360GCTCTAACGC CATCAAGTGG CTGTAATTTT GGTTGGAACG CATTGATAAA TAAAAACATA420GATGTATCCA TGGATAGCGT GCTAGACAAT TTGAGCAACA AAATTCAGAA TTTTACCAAA480GGCGGTGTTG AGGACAATGT GAAAGGCAAT ATTCTTTTAC AAATAATTGG CTCAATAACC540GCTCAAGCTT CTACGAATAT TACAGCTGAT GGTTTAATTT GGCTGATTGG TAAAGAATTC600ACTGCAAATA AACTGCAAAA CAACACTACA GCCATGCTTG CTTTTGCCGC ATTAGAATCT660GTTGTCAAAG GAGCGGACGC TGCTGTTCTT CCTGCATATG GTGTAGTCAA TCTGCCTGAT720ATTATCATAG GGCAAGGGTC ATATCTTGAT TTTGTTTCTT ACCTAATTTA TATCGTTTTT780GGGATTTTTG TTTTTATTTC TTTTATGAAA TTGAGGGATA TTTCAAGTAA CATTCAGATT840AACATAGGTT TTGAATACAT GCGATTTGTT GGGGGGACAT TATTCAAAAT GGCGATGGTC900TCTTTTATCG CCTATGCAGG TTTTGGTTAT CTTTATAAAA TCTCTTATTC TATTTATTTT960GGTTTAGCAG GCGCTTTTGG GCTGAATCAA GTTCTTTTTT GGGCTTTAGA TTTAGTGCTG1020AATTACACTG TTAATTCAAT TTTACCTGCG GTAAGAGCTG TTTTTTCTAA TGTTGGCAAC1080AACGCTCCTA GTTTGTTACA AGGCTTGCAA GTGGCAGGTA TTTCTTTATT CGCCATTTTT1140ATGCAAGTAA CTATCATTAT GAGAATAAGC ACTGTTGTTG TGAAACCTTT GATAGCTGGG1200GCTTTTAGCG GTATTGTGTT CCCTATTGCA GTAAGTTTGA TCGTGCTAGA TTGGTTCAAA1260GATTCTATGA AAAACATATT GATATGGTTT ATTAATAATT TGTTTATCTT GGTTCTAGCT1320ATTCCTATTT TGCTCTTTGG TGTTTTGGCA TTATTGGCAT TCAATTTGAC CATAACGCCC1380TCTGTTGCTA TACAAAACAT CAATCAAGGG GGACTAGGTA TCGATTCAAC TATTGCGAGT1440TTGATCACCC TATTTATTTT AAAAGGTTTC ATAGAGACGA TTATTGAGAG CGTCAATGCG1500ATCGTTAATA CCATTTTTAG CTCTGTTTCT ATGGATGGTA GCAGAATGGA TAGAGAAAGA1560GATGCCTTAA TGGTTGGAAG AGTTGGTGGA TCTATGTTTA AAGGATAA 1608
<210>10<211>759<212>DNA<213>HELICOBACTER PYLORI<400>10ATGTTAGGGA AAAAAAACGA AGAAGTCTTG ATTGATGAAA ATTTGGTTGG GGGTGTGATA60GCCCTTGATA GATTGGCAAA ACTCAATAAG GCCAATAGGA CTTTCAAAAG GGCTTTTTAT120CTCTCTATGG CACTCAATGT CGCCGCTGTA ACGAGTATTG TGATGATGAT GCCTTTGAAG180AAAACGGATA TATTTGTTTA TGGCATTGAT CGATACACAG GAGAATTTAA AATTGTCAAA240CGCTCCGATG CTAGGCAAAT CGTCAATTCT GAAGCCGTTG TGGATAGTGC AACTTCAAAA300TTTGTATCAT TGCTGTTTGG TTATAGCAAA AATTCTTTGA GGGATCGCAA GGATCAACTA360ATGCAGTATT GCGATGTGAG TTTCCAAACC CAAGCAATGA GAATGTTCAA TGAAAATATC420AGACAATTCG TAGATAAAGT CCGAGCAGAA GCTATCATTA GCTCTAACAT ACAAAGAGAA480AAAGTCAAAA ATAGTCCCTT AACGAGATTA ACATTTTTCA TTACCATCAA AATCACACCT540GATACAATGG AAAATTATGA ATATATCACT AAAAAACAAG TAACTATTTA TTATGATTTT600GCTAGAGGTA ACTCTTCTCA AGAAAATCTT ATCATCAATC CTTTTGGCTT CAAAGTGTTT660GACATTCAAA TCACAGATTT ACAAAACGAA CAGACGGTAA GCGAAATTTT GAGAAAGATC720AGAGAAGTGG AATCAAAAAA TAAGGCATTA AATAAATAA 759<210>11<211>657<212>DNA<213>HELICOBACTER PYLORI<400>11ATGAACGATA CAACAGAGCA TCATGGACCT AATCCGCTAA ACGCTCCACC ACCTAGCAAC60TCACAGAGCA ATGATCTTTT AAATTTGCTA GACTCATTAT ATCCTAAAGG GAGTTTAGGG120GAGCAAAGAT TTCACGAAGC TTTAAAGAAT CAAGAAGAGT TGAAAAATAT CCTAATAGAA180ATAGAAAAGC TACCGCAAGA AAAAAGGTAT GAACTTCTGA TGCAGATAGG ACAAGCCAAG240CAAAGAATAA TGGAAGCATA TGCTCATTCA TTCTTAGGAT ATATAGGGGG ACTAGAGCAT300CTGTTAGGAT TGTGTATGGG TGGGATATTT GTTTTGTTTG CAATCTATTT TGTATTTTTA360
AGAACTAGCA AAAACATGGA GCTAGTGGAA AGTCTAAAAA CAAAACTAAA ACTTCAGTAT420TTTTACTATG CCTTTGGTGT GGGTGCGGTT TTGTTTTTTG GATTAGAAAC AATTAGATCT480ATTTATGAAC TATATATCTT AGGAATTGGT AGCACTAACG ACAAGGTGCT CTTTGTTTTG540AAAAACATTT GCTTCATAGG TATGGGCTAT TTGATTTATA AAGTTATTAA GGTTATTGGT600ATAAAAAATT TTATCAATGG TCTTTTCACT TCAAAGAAAC AAGGCGGTGC AGAATGA 657<210>12<211>839<212>DNA<213>HELICOBACTER PYLORI<400>12ATGAAACTGA GAGCAAGTGT TTTAATCGGT GCGACAATTC TGTGCTTAAT TTTAAGCGCA60TGCAGTAATT ATGCGAAAAA AGTGGTGAAA CAAAAGAACC ATGTTTATAC GCCTGTGTAT120AATGAACTGA TAGAGAAGTA TAGTGAGATA CCCTTAAATG ACAAACTCAA AGACACACCA180TTCATGGTGC AAGTGAAGTT GCCAAATTAC AAGGACTATT TGTTGGATAA TAAACAAGTT240GTACTAACTT TCAAACTTGT TCACCATTCT AAAAAGATTA CGCTCATAGG CGATGCCAAT300AAGATACTTC AATACAAGAA TTACTTCCAA GCTAATGGAG CGAGATCTGA CATTGATTTT360TACTTGCAAC CCACTTTGAA TCAAAAGGGT GTGGTGATGA TAGCGAGTAA CTACAATGAT420AATCCTAACA GCAAAGAAAA ACCACAGACC TTTGATGTGT TGCAAGGAAG TCAGCCAATG480CTAGGAGCTA ACACAAAAAA CTTGCATGGC TATGATGTGA GTGGAGCAAA CAACAAGCAA540GTGATCAATG AAGTGGCAAG AGAAAAAGCT CAGCTAGAAA AAATCAATCA GTATTACAAA600ACTCTTTTAC AAGACAAGGA ACAAGAATAT ACCACTAGGA AAAATAACCA ACGAGAAATT660TTAGAAACAT TGAGTAATCG TGCAGGTTAT CAAATGAGGC AGAATGTGAT TAGTTCTGAG720ATTTTTAAGA ATGGCAACTT GAACATGCAA GCCAAAGAGG AAGAAGTTAG GGAGAAGCTA780CAAGAAGAAA GAGAGAATGA ATACTTGCGA AATCAAATCA GAAGTTTGCT CAGTGGTAAG840TGA 843<210>13<211>591<212>DNA<213>HELICOBACTER PYLORI
<400>13ATGAGTAATA ACATGCGAAA ACTCTTCTCA ATGATTGCTG ACTCAAAAGA TAAGAAAGAA60AAACTTATTG AGAGTTTGCA AGAGAACGAA CTTTTAAGCA CTGATGAAAA AAAGAAAATC120ATAGATCAAA TAAAAACCAT GCATGATTTT TTCAAACAGA TGCATACAAA CAAGGGAGCG180TTAGATAAGG TTCTAAGAAA TTACATGAAA GATTATCGCG CTGTTATCAA AAGCATTGGT240GTTGATAAGT TTAAAAAGGT TTATCGATTG CTTGAGAGTG AGACTATGGA GCTGTTGCAT300GCCATTGCAG AGAATCCTAA TTTCTTATTC TCCAAATTTG ATCGATCAAT TCTTGGAATA360TTTCTGCCTT TCTTCAGTAA GCCTATCATG TTCAAGATGA GTATTAGAGA AATGGACTCG420CAAATAGAAT TGTATGGCAC TAAGCTCCCA CTATTGAAAT TGTTTGTGAT GACAGATGAA480GAAATGAATT TCTACGCTAA TCTAAAAACC ATTGAACAAT ATAACGACTA TGTTAGGGAT540TTGCTGATGA AATTTGATCT TGAAAAATAC ATGAAAGAAA AAGGAGTGTA A 591<210>14<211>381<212>DNA<213>HELICOBACTER PYLORI<400>14ATGCTTCCTA CTAAAACACG CATTAGAGAT CCGAACAAGC AAGAACTTAC ACAACCAAAA60ATAAAAGGAC TGATCATGGG AAAAATTTTA GCTTCTTTGT TGGGTGGCGG AACAAATCTT120TTTACAGGTT TATCCAGTGA TTTGTTTTCT ATGATATTAA ATTTTTTGTT CTTCCTGATG180TTAATGATGG GACTTAATGA AATATTAGGG AAAAAATTCA ACTTGCCTAT GGACAATATC240AAGAATTTTA TGGCAGAAGT GTTGAAGAAT GGATTCGATA GTATCAAAAA CATGGGATCT300GCTTTGGTTG GTAATGGTTT TGGTAGCAAC AAATCAGACA AAACCACTAA TAAAATGAGT360GTCTCTCAAG TAAGACTCTA G 381<210>15<211>345<212>DNA<213>HELICOBACTER PYLORI<400>15
ATGAAACGAC CGATTAGCAA GTTAAAACAA AACTTTTTAC AATTCAAACA TTCTTTCAAC60AAACATTTAG ATAAATACAG CCTTTATTAT AGGCTGTTCA ATATCAGTTC TATCGTTATA120GGTTTTTTAG TAGCCCTTTT TTCTTATGGG GCAGGGGTGA TTTTAGTTTA TCCAATATTA180TTCCTATTTG CTCTTATAAT AAAACCTAGC TTTTTTTATT ACACTACTTA TCTTTTGCTA240CTCGTTTCTC TCAGCATAAT AAGCAAATAC TATCTCTTAA GCCACGCAAA TTTCACAATG300AAGCTAATCA TACTTATGAC TCAATGGCAA AATTGGTTCT TATGA345<210>16<211>1131<212>DNA<213>HELICOBACTER PYLORI<400>16ATGCTTGCAA AAATCGTTTT TAGCTCATTG GTTGCGTTTG GAGTTTTGTC GGCTAATGTG60GAGCAGTTTG GTTCATTTTT CAACGAGATA AAAAAAGAAC AAGAAGAAGT GGCTGCAAAA120GAAGACGCTC TTAAGGCTCG CAAGAAGCTC TTAAACAATA CGCATGATTT CTTAGAAGAC180TTGATTTTTA GAAAACAAAA AATCAAAGAG CTTATGGATC ATAGAGCTAA AGTTCTTTCA240GACTTAGAAA ACAAATACAA AAAAGAAAAA GAGGCTCTAG AGAAAGAGAC AAGAGGTAAA300ATCCTTACTG CTAAGTCAAA GGCTTATGGG GATTTGGAGC AAGCCTTAAA AGATAACCCT360CTCTATAGGA AACTTCTTCC TAACCCTTAT GCCTATGTTT TAAACCAAGA AACATTCACC420AAAGAAGATA GGGAGCGTTT GAGTTATTAC TACCCCCAGG TGAAAACGAG CAGTATTTTT480AAAAAAACCA CCGCTACCAC TAAAGATAAG GCTCAGGCTT TGCTTCAAAT GGGTGTGTTT540TCTTTAGATG AAGAACAAAA CAAAAAAGCG AGCCGATTAG CTTTATCTTA CAAGCAAGCG600ATTGAAGAAT ATTCCAATAA CGTTTCTAAT TTATTGAGCA GAAAAGAATT GGATAATATA660GATTATTACT TACAGCTTGA AAGAAACAAG TTTGACTCCA AAGCAAAAGA TATTGCTCAA720AAGGCTACTA ACACGCTTAT TTTTAATTCG GAACGCTTGG CGTTTAGCAT GGCGATTGAT780AAGATCAATG AGAAATACTT AAGGGGCTAT GAGGCTTTTT CTAACTTGTT GAAAAATGTC840AAAGATGATG TGGAATTGAA TACTCTGACT AAAAATTTTA CCAATCAAAA ATTGAGTTTC900GCACAAAAAC AAAAATTGTG TTTGTTGGTT TTAGACAGCT TCAATTTTGA TACCCAATCC960AAAAAATCTA TATTAAAAAA GACTAATGAA TACAATATTT TCGTAGATAG CGATCCTATG1020ATGAGCGACA AAACCACTAT GCAAAAAGAA CACTACAAGA TATTTAATTT CTTCAAAACA1080GTGGTTTCTG CATACCGAAA CAATGTTGCC AAGAATAATC CCTTTGAATA G 1131
<210>17<211>921<212>DNA<213>HELICOBACTER PYLORI<400>17TTGAAAAGTA TCTTCAAAAA ACTAGGTTCT GTCGCTCTTT ATTCTTTAGT TATTTATGGG60GGCTTAAACG CTATCAATAC AGCATTATTG CCGAGTGAAT ACAAAGAATT AGTGGCTTTG120GGCTTTAAAA AAATCAAAAC ACTCCATCAA AGACATGACG ACGAAGAAGT TACAAAAGAG180GAAAAAGAAT TCGCCACTAA CGCTTTGAGA GAAAAATTAC GAAATGATAG GGCAAGAGCA240GAGCAAATTC AAAAGAATAT TGAAGCGTTT GAAAAAAAGA ACAACTCTTC TATTCAAAAA300AAAGCGGCTA AGCACAAAGG ATTACAAGAA TTAAACGAAA TTAACGCTAC CCCTTTGAAT360GACAACCCTA ATAGCAATTC TTCCACTGAA ACCAAATCTA ATAAAGATGA TAACTTTGAT420GAGATGATCA ATAAGGTGAA TGGAGCTTTT GTGAAACCTG CTACTCCGCT TGTGCCTGAT480GAGTGGAGAA CGCCTGAAAT TGAAATCATT ATCAATGAGT GTATTATTTC AAGCAACGAT540TATGATGGGT TAAGAAAGTG TTTGATCAAA GGCATCAAGG ATCAAAAAAT TCTTGCCCCC600TTATTAGAAA AAATTCAAGA AATAGAGACA GAAAATAACA AATTTTCTAG ACAACACTTG660AGTGGTTTAA AACTCGCTCT TAATAACAGC AACAATAGAA CCTTTCTTAT AGCTTCGTGC720GCTATTTGTG AGAAGAGAAA AAAAGAAATG GAGCAAGAAA ATAACTACCA AGATACTACA780AATGCAAGTG AGTTTGGAGC TACTGATACA AAAGAAAATG AAGCAAAAGA TGCAGCATTC840TCAAACAATC GCTCTAAATC TGAACTGCCC AATAGCGTCA TTAATCAAAT AGAACAAAGC900ATCGCTCATG GGAAAAAATA G 921<210>18<211>714<212>DNA<213>HELICOBACTER PYLORI<400>18ATGAAAACAC TCGTGAAAAA TACCATATCT TCTTTTTTGC TCTTGTCTGT TTTGATGGCA60GAAGATATAA CAAGCGGTTT AAAGCAACTG GATAGCACCT ACCAAGAGAC CAACCAACAA120
GTGCTCAAAA ACTTAGATGA GATTTTTTCA ACCACTAGCC CTAGTGCTAA TAATGAAATG180GGTGAAGAAG ATGCTCTAAA CATCAAAAAA GCGGCCATTG CTTTGAGAGG AGATTTAGCG240TTATTGAAAG CCAATTTTGA AGCGAATGAG TTATTTTTCA TCTCAGAAGA TGTGATTTTC300AAAACTTATA TGTCTAGCCC TGAACTTTTA TTAACCTATA TGAAAATCAA TCCCTTAGAC360CAAAATACTG CTGAGCAACA ATGCGGAATA TCCGATAAAG TTTTAGTTCT TTATTGTGAA420GGGAAGCTGA AAATCGAGCA AGAAAAACAA AATATAAGAG AGCGTTTAGA AACTTCTCTA480AAGGCATATC AGAGCAACAT TGGAGGTACA GCTTCCTTAA TCACTGCTTC ACAGACGCTT540GTAGAAAGCC TAAAAAATAA AAATTTCATC AAAGGAATCA GAAAGCTTAT GTTAGCTCAC600AACAAGGTCT TTTTAAATTA TTTAGAGGAG TTGGACGCAT TAGAAAGATC CCTAGAACAA660AGTAAGCGAC AATACCTACA AGAAAGGCAA TCAAGTAAGA TCATTGTTAA ATGA 714<210>19<211>1146<212>DNA<213>HELICOBACTER PYLORI<400>19GTGAAATGTT TTTTAAGCAT ATTTTCTTTC TTGACTTTTT GTGGTTTGTC TCTGAATGGC60ACAGAAGTAG TAATAACGCT TGAACCCGCC TTAAAAGCCA TTCAGGCGGA CGCACAAGCC120AAACAAAAAA CCGCTCAAGC TGAATTAAAA GCCATAGAAG CTCAGTCTAG TGCCAAAGAA180AAAGCCATTC AAGCGCAAAT AGAGGGAGAA TTGAGGACTC AGCTTGCAAC CATGAGTGCT240ATGTTAAAAG GGGCTAATGG CGTTATTAAT GGTGTCAATG GCATGACAGG GGGGTTTTTT300GCAGGTTCAG ACATCTTGCT TGGCGTCATG GAAGGGTATT CAAGCGCGCT TAGTGCATTG360GGGGGGAATG TTAAAATGAT CGTGGAAAAA CAAAAAATTA ACACCCAAAC AGAAATCCAA420AACATGCAAA TCGCGCTCCA AAAAAATAAC GAAATAATCA AGCTCAAAAT GAACCAGCAA480AACGCTCTCT TAGAAGCGTT AAAAAATAGC TTTGAACCGA GCGTTACTCT AAAAACACAA540ATGGAAATGC TTTCTCAAGC TCTAGGAAGT TCTTCTGACA ACGCTCAATA CATCGCTTAC600AATACGATTG GTATCAAGGC GTTTGAAGAA ACCTTAAAAG GTTTTGAAAC ATGGTTGAAA660GTGGCTATGC AAAAAGCGAC CCTTATTGAT TATAATTCCC TAACGGGTCA GGCTTTGTTT720CAAAGTGCCA TCTATGCGCC TGCTCTTAGT TTTTTTTCAA GCATGGGTGC ACCATTTGGA780ATCATTGAAA CATTCACTCT AGCGCCCACA AAATGCCCCT ATCTTGATGG GCTAAAAATT840TCAGCATGCC TTATGGAACA GGTTATTCAG AATTACAGAA TGATTGTAGC CCTTATTCAA900
AATAAACTGA GTGATGCAGA TTTTCAAAAT ATCGCTTATT TGAATGGGAT CAATGGAGAA960ATCAAAACCT TAAAAGGATC AGTAGATTTG AACGCGCTCA TAGAAGTTGC TATCTTAAAC1020GCAGAAAATC ATTTAAACTA TATAGAGAAT CTTGAAAAAA AAGCCGACCT TTGGGAAGAA1080CAACTGAAAT TAGAAAGAGA AACGACAGCA AGAAACATTG CTAGCTCTAA AGTTATTGTC1140AAATGA 1146<210>20<211>1113<212>DNA<213>HELICOBACTER PYLORI<400>20ATGGCAGGTA CACAAGCTAT ATATGAATCA TCTTCTGCAG GATTCTTATC GGAAATTTCC60TCAATCATCT CAAGCACAAG TGGTGTTGCA GGGCCATTTG CAGGAATAGT AGCGGGTGCT120ATGTCAGCAG CGATTATTCC TATTGTTGTG GGATTTACTA ATCCGCAAAT GACCGCTATC180ATGACCCAAT ACAATCAGAG CATCGCTGAA GCCGTAAGCA TGCCTATGAA AGCTGCTAAC240CAACAATACA ACCAATTGTA TCAAGGTTTT AACGATCAAA GCATGGCTGT GGGGAACAAT300ATCTTAAATA TCAGCAAATT AACAGGGGAA TTTAACGTGC AAGGCAACAC GCAAGGCGCG360CAAATTAGTG CCGTTAATAG TCAGATTGCA AGCATTTTAG CGAGTAACAC CACCCCTAAA420AATCCTAGCG CTATTGAAGC TTATGCGACA AATCAAATCG CTGTTCCTAG TGTGCCAACA480ACGGTTGAAA TGATGAGCGG TATCTTAGGC AATATTACAA GCGCGGCACC AAAATACGCC540CTAGCTCTAC AAGAGCAATT GCGTTCTCAA GCAAGCAACA GCTCAATGAA TGATACAGCC600GATTCCCTTG ATAGCTGTAC CGCTTTAGGT GCACTTGTTG GCTCATCAAA AGTGTTTTTT660AGTTGCATGC AAATTTCTAT GACGCCCATG AGTGTTTCTA TGCCCACTGT TTATGCCAAA720TACCAAGCGT TASCNACTAA TGCCCTAACT TCAGGCACTA ATCCTATGAC CACTCCTGCA780TGCCCTATTG GGGACAAAGT TCTTGCCGTT TATTGCTATG CTGAAAAAGT AGCAGAAATT840TTGAGGGAAT ACTATATAGA ATTTGTGAAA AACAATACCA ATTTGTTGCA AAACGCTTCT900CAAATGATAC TTAATCAATC AGGATTAGCT ACTAGCACCT ATGACACTCA AGCGATTTCT960AACATAAGCT CGCTATATAA TTACAATATA GTAGCGAATA AATCTTTTTT GAAATCGCAT1020TTGACTTACC TTGATTACAT CAAAAACAAG CTTAAGGGGC AAAAAGATAG CTACTTAACA1080GAAAGGGTGC AAACTAAAAT AATCGTGAAG TGA 1113
<210>21<211>429<212>DNA<213>HELICOBACTER PYLORI<400>21ATGAAAACGA ATTTTTATAA AATTAAATTA CTATTTGCTT GGTGTCTTAT CATTGGCATG60TTTAACGCTC CGCTTAATGC TGACCAAAAC ACGGATATAA AAGATATTAG TCCTGAAGAT120ATGGCACTAA ATAGCGTGGG GCTTGTTTCT AGAGATCAAC TAAAAATAGA GATCCCTAAA180GAAACCCTAG AACAAAAAGT GGCCGTACTC AATGACTATA ATGATAAGAA TGTTAATATC240AAGTTTGACA ACATAAGTTT AGGGAGTTTT CAACCTAATG ATAATCTAGG TATCAATGCG300ATGTGGGGCA TTCAAAATCT TCTCATGAGC CAAATGATGG GCGATTACGG TCCAAACAAT360CCTTTCATGT ATGGTTATGC ACCAACATAC TCAGATTCAT CATTTTTACC ACCGATCTTA420GGGTATTAA429<21O>22<211>807<212>DNA<213>HELICOBACTER PYLOR<400>22ATGAAACAAA GTTTGCGCGA ACAAAAATTA TTGAAAATTT TAGAAAATGA TGTCTTGACG60ATTTTGGATA GTTTTTCTAA TTATCTTTTT GAACTGAGAG AAGAGTTGGA CTTCATAGAA120GAAGAAATGG AAGGTGAAAT CACCGAACAA AACCTTACCG CTCTTTATGA TTTTTCTAAT180TTCTTAGAAG ACCATGTCAA TGTATTTTAT GAGAATGTTT TGAATATAGA TGATGTCAAA240ACAGAACACC TTTATTCAGG TCTCATAGAT AGTCTTAACG CTAATCTTCA CTTTGTCAAG300TCATTTCTCA GTAATCAGGA TTTAGACTTC CGCTTTTTTA AGGAAATAAA CGATGGGCAA360GATCCCCAAA AAACATTATC AAGATTAATT CCTCTTCAAA GTGGGAAAAA TGATGCAAGC420TCGTTTAAAG CCAATAATTC TTTTGTTTCA TTAGTTTATG TTTATGTTTA CTTCATGCTA480GAAACTATCA TGCAGTCGTA TAGGATTCTC AGATTGCTAG AAAAACCTAT CAATAACAAC540ATAAGCGAGG ACATGCAGAA CGATATAGAG AATTTTTTTG TTCAAGCGAA TTTTTTAGAA600TACTATGTTC AGAACAAAAT ATACCCAACC AATCATGCCT ATGACTTCAC GCATTTGATC660ATGGACTCCA TTATTCCTAA TTGGATTCAA ACTGATATGA GCGTTGAAGC TAAAAAGAAA720
GAGCTTTTTG AAAAATATTT TCAAAACATT GATGAAGTAA CAAACAAAAT GCTCGATCAA780AAAAATCAAA ACAANAGTAA CGATTGA807<210>23<211>2952<212>DNA<213>HELICOBACTER PYLORI<400>23GTGTTTGTGG CAAGCAAACA AGCTGACGAA CAAAAAAAGC TAGTCATAGA GCAAGAGGTT60CAAAAGCGCC AATTTAAAAA AATAGAAGAA CTTAAAGCAG ACATGCAAAA GGGTGTCAAT120CCCTTTTTTA AAGTCTTGTT TGATGGGGGG AATAGGTTGT TTGGTTTCCC TGAAACTTTC180ATTTATTCCT CTATATTTAT ATTGTTTGTA ACAATTGTAC TATCTGTTAT TCTTTTTCAA240GCCTATGAAC CTGTTTTGAT TGTAGCGATT GTTATTGTGC TTGTAGCTCT TGGATTCAAG300AAAGATTATA GGCTTTATCA AAGAATGGAG CGAGCGATGA AATTTAAAAA ACCTTTTTTG360TTTAAGGGCG TGAAAAACAA AGCGTTCATG AGCATTTTTT CCATGAAGCC TAGTAAAGAA420ATGGCTAATG ACATCCACTT AAATCCAAAC AGAGAAGACA GGCTTGTGAG CGCTGCAAAC480TCCTATCTAG CGAATAACTA TGAATGTTTT TTAGATGATG GGGTGATCCT TACTAACAAC540TATTCTCTTT TAGGCACAAT CAAATTGGGG GGCATTGATT TTTTAACCAC TTCCAAAAAA600GATCTCATAG AGTTACACGC TTCTATTTAT AGCGTTTTTA GGAATTTTGT TACCCCTGAA660TTCAAATTCT ATTTTCACAC TGTTAAAAAG AAAATCGTTA TTGATGAAAC CAATAGGGAC720TATAGTCTTA TTTTTTCTAA TGATTTCATG CGAGCCTATA ATGAGAAGCA AAAGAGAGAA780AGTTTTTATG ATATTAGTTT TTATCTCACC ATAGAGCAAG ATTTATTAGA CACTCTCAAT840GAACCCGTTA TGAATAAAAA GCATTTTGCA GACAATAATT TTGAAGAGTT TCAAAGGATT900ATTAGAGCCA AGCTTGAAAA CTTCAAGGAT AGGATAGAGC TCATAGAAGA GCTATTGAGT960AAATACCACC CCATTAGATT AAAAGAATAC ACTAAAGATG GCGTTATTTA CTCCAAACAA1020TGCGAGTTTT ATAATTTCCT TGTGGGAATG AATGAAGCCC CTTTTATTTG CAACAGAAAA1080GACTTGTATC TCAAGGAAAA AATGCATGGT GGGGTGAAAG AAGTTTATTT TGCCAATAAG1140CATGGAAAAA TCTTAAATGA CGATTTGAGT GAAAAATATT TTAGCGCTAT TGAGATTAGT1200GAATACGCCC CTAAATCACA AAGCGATTTG TTTGATAAAA TCAACGCCCT AGACAGCGAA1260TTTATTTTCA TGCATGCTTA TTCGCCTAAA AACTCACAGG TTTTAAAGGA CAAACTAGCT1320TTCACCTCTA GAAGAATTAT TATTAGTGGA GGCTCTAAAG AGCAGGGCAT GACTTTAGGT1380
TGCTTGAGCG AATTAGTGGG TAATGGTGAT ATTACGCTAG GCAGTTATGG TAATTCTTTA1440GTGTTGTTTG CTGATAGCTT TGAAAAAATG AAACAAAGCG TTAAGGAATG CGTCTCTAGT1500CTTAACGCTA AAGGTTTTTT AGCCAACGCA GCGACTTTCT CTATGGAAAA TTACTTTTTC1560GCCAAACATT GCTCTTTTAT CACGCTTCCT TTTATTTTTG ATGTAACTTC TAATAATTTT1620GCTGATTTCA TCGCTATGAG GGCTATGAGT TTTGATGGCA ATCAGGAGAA TAACGCTTGG1680GGCAATAGTG TGATGACGCT AAAAAGCGAG ATCAATTCGC CTTTTTATCT GAACTTCCAC1740ATGCCTACTG ATTTTGGTTC AGCTTCAGCA GGACACACTT TGATACTTGG CTCAACCGGT1800TCAGGTAAGA CAGTGTTTAT GTCAATGACC TTGAACGCTA TGGGGCAATT TGTTCACAAT1860TTTCCTGCTA ATGTCAGCAA AGACAAGCAA AAGCTCACTA TGGTTTATAT GGATAAAGAT1920TATGGCGCTT ATGGGAATAT TGTCGCAATG GGTGGGGAGT ATGTCAAGAT TGAGCTAGGG1980ACAGATACAG GATTAAATCC TTTTGCTTGG GCGGCTTGTG TGCAAAAAAC AAATGCAACA2040ATGGAGCAAA AACAAACAGC TATTTCTGTT GTCAAAGAGC TTGTGAAAAA CTTAGCAACC2100AAAAGCGATG AAAAAGATGA GAATGGCAAC AGCATCTCTT TTAGCCTAGC AGATTCTAAT2160ACGCTTGCAG CGGCAGTAAC CAACCTTATC ACAGGAGATA TGAACCTAGA TTATCCCATC2220ACTCAACTTA TTAATGCTTT CGGGAAAGAC CACAATGATC CTAATGGGCT TGTCGCGCGA2280TTAGCACCTT TTTGCAAATC AACCAATGGT GAATTTCAAT GGCTTTTTGA TAATAAAGCA2340ACGGATCGCT TAGATTTTTC AAAAACGATT ATTGGCGTTG ATGGGTCAAG TTTCTTAGAC2400AATAATGATG TTTCGCCCTT TATTTGTTTT TACCTTTTCG CTCGTATCCA AGAGGCAATG2460GATGGGCGTA GATTTGTCTT AGATATTGAT GAAGCTTGGA AATATTTAGG CGATCCAAAG2520GTCGCTTATT TTGTAAGAGA CATGCTAAAA ACTGCAAGGA AAAGAAACGC TATTGTCAGA2580CTTGCGACTC AAAGCATCAC TGATCTTTTG GCTTGCCCTA TTGCTGATAC GATTAGAGAA2640CAATGCCCTA CAAAGATTTT TTTGAGAAAC GATGGGGGCA ATCTTTCTGA TTACCAAAGA2700TTGGCTAATG TTACAGAAAA AGAATTTGAA ATCATCACTA AGGGACTAGA TAGGAAAATT2760CTCTACAAAC AGGATGGAAG CCCTAGCGTT ATCGCTAGTT TTAATTTGAG GGGCATTCCT2820AAAGAATATT TGAAAATTTT ATCCACAGAT ACTGTATTTG TCAAAGAAAT TGACAAGATT2880ATCCAAAACC ATAGTATCAT AGATAAATAT CAGGCCTTGA GGCAAATGTA TCAACAAATA2940AAGGAGTATT AA2952<210>24
<211>624<212>DNA<213>HELICOBACTER PYLORI<400>24TTGATCAATA ATAATAGTAA TAAAAAACTG AGAGGCTTTT TTGTGAAAGT TCTCTTAAGT60CTCGTTGTTT TCAGTTCGTA TGGGTTAGCA AATGACGATA AAGAAGCCAA AAAAGAAGTG120CTAGAAAAAG AAAAAAACAC TCCCAATGGG CTTGTTTATA CGAATTTAGA TTTTGATAGT180TTCAAGGCGA CTATCAAAAA TTTGAAAGAC AAGAAAGTAA CTTTCAAAGA AGTCAATCCC240GATATTATCA AAGATGAAGT TTTTGACTTC GTGATTGTCA ATAGAGTCCT TAAAAAAATA300AAGGATTTGA AGCATTACGA TCCAGTTATT GAAAAAATCT TTGATGAAAA GGGTAAAGAA360ATGGGATTGA ATGTGGAATT GCAGATCAAT CCTGAAGTGA AAGACTTTTT TACTTTTAAA420AGCATCAGCA CGACCAACAA ACAACGCTGC TTTCTATCGT TGCGCGGGGA AACAAGAGAA480ATTTTATGCG ATGATAAGCT GTATAATGTT TTATTGGCCG TATTCAATTC TTATGACCCT540AATGATCTTT TGAAACATAT TAGCACCGTA GAGTCTCTCA AAAAAATCTT TTATACGATT600ACATGTGAAG CGGTATATCT ATAA 624<210>25<211>348<212>DNA<213>HELICOBACTER PYLORI<400>25ATGAAATTTT TTACAAGAAT CACTGACAGC TACAAGAAAG TTGTAGTAAC TTTAGGGCTA60GTGGTAACAA CCAATCCTTT AATGGCGGTC ACCAGTCCTG CAGAAGGCGT TACTGAGACT120AAAACTTTGG TTATTCAGAT CATTTCTGTT CTAGCGATCG TAGGGGGTTG CGCTTTAGGG180GTCAAAGGCA TAGCGGATAT TTGGAAAATC TCTGATGATA TCAAAAGAGG TCAGGCGACT240GTTTTTGCTT ACGCGCAACC CATAGCTATG TTAGCGGTGG CAGGCGGTAT TATCTATTTG300AGCACTAAGT TTGGCTTCAA TATTGGCGAG AGCGGAGGAG CTAGCTAA 348<210>26
<211>3561<212>DNA<213>HELICOBACTER PYLORI<400>26ATGACTAACG AAACTATTGA TCAAACAAGA ACACCAGATC AAACACAAAG CCAAACAGCT60TTTGATCCGC AACAATTTAT CAATAATCTT CAAGTGGCTT TTATTAAAGT TGATAATGTT120GTCGCTTCAT TTGATCCTGA TCAAAAACCA ATCGTTGATA AGAACGATAG GGATAACAGG180CAAGCTTTTG ATGGAATCTC GCAATTAAGG GAAGAATACT CCAATAAAGC GATCAAAAAT240CCTACCAAAA AGAATCAGTA TTTTTCAGAC TTTATCGATA AGAGCAATGA TTTAATCAAC300AAAGACAATC TCATTGATGT AGAATCTTCC ACAAAGAGCT TTCAGAAATT TGGGGATCAG360CGTTACCAAA TTTTCACAAG TTGGGTGTCC CATCAAAAAG ATCCGTCTAA AATCAACACC420CGATCGATCC GAAATTTTAT GGAAAATATC ATACAACCCC CTATCCCTGA TGATAAAGAA480AAAGCAGAGT TTTTGAAATC TGCCAAACAA TCTTTTGCAG GAATCATTAT AGGGAATCAA540ATCCGAACGG ATCAAAAGTT CATGGGCGTG TTTGATGAAT CCTTGAAAGA AAGGCAAGAA600GCAGAAAAAA ATGGAGGGCC TACTGGTGGG GATTGGTTGG ATATTTTTCT CTCATTTATA660TTTAACAAAA AACAATCTTC CGATGTCAAA GAAGCAATCA ATCAAGAACC AGTTCCCCAT720GTCCAACCAG ATATAGCCAC TACTACCACC GACATACAAG GCTTACCGCC TGAAGCTAGG780GATTTACTTG ATGAAAGGGG TAATTTTTCT AAATTCACTC TTGGCGATAT GGAAATGTTA840GATGTTGAGG GAGTCGCTGA CATTGATCCT AATTACAAGT TCAATCAATT ATTGATTCAC900AATAACGCTC TGTCTTCTGT GTTAATGGGG AGTCATAATG GCATAGAACC TGAAAAAGTT960TCATTATTGT ATGCGGGCAA TGGTGGTTTT GGAGACAAAC ACGATTGGAA CGCCACCGTT1020GGTTATAAAG ACCAACAAGG TAACAATGTG GCTACACTCA TTAATGTGCA TATGAAAAAC1080GGCAGTGGCT TAGTCATAGC AGGTGGTGAG AAAGGGATTA ATAACCCTAG TTTTTATCTC1140TACAAAGAAG ACCAACTCAC AGGCTCACAA CGAGCATTGA GTCAAGAAGA GATCCGAAAC1200AAAGTAGATT TCATGGAATT TCTTGCACAA AATAATACTA AATTAGACAA CTTGAGCGAG1260AAAGAGAAAG AAAAATTCCA AAATGAGATT GAAGATTTTC AAAAAGACTC TAAGGCTTAT1320TTAGACGCCC TAGGGAATGA TCGTATTGCT TTTGTTTCTA AAAAAGACAC AAAACATTCA1380GCTTTAATTA CTGAGTTTAA TAATGGGGAT TTGAGCTACA CTCTCAAAGA TTATGGGAAA1440AAAGCAGATA AAGCTTTAGA TAGGGAGAAA AATGTTACTC TTCAAGGTAG CCTAAAACAT1500GATGGCGTGA TGTTTGTTGA TTATTCTAAT TTCAAATACA CCAACGCCTC CAAGAATCCC1560AATAAGGGTG TAGGCGCTAC GAATGGCGTT TCCCATTTAG AAGCAGGCTT TAACAAGGTA1620
GCTGTCTTTA ATTTGCCTGA TTTAAATAAT CTCGCTATCA CTAGTTTCGT AAGGCGGAAT1680TTAGAGAATA AACTAACCGC TAAAGGATTG TCCCTACAAG AAGCTAATAA GCTCATCAAA1740GACTTTTTGA GCAGCAACAA AGAATTGGCT GGAAAAGCTT TAAACTTCAA TAAAGCTGTA1800GCTGAAGCTA AAAGCACAGG CAACTATGAC GAGGTGAAAA AAGCTCAGAA AGATCTTGAA1860AAATCCCTAA GGAAACGAGA GCATTTGGAG AAAGAAGTAG AGAAAAAATT GGAGAGCAAA1920AGCGGCAACA AAAATAAAAT GGAAGCAAAA GCTCAAGCTA ACAGCCAAAA AGATGAGATT1980TTTGCGTTGA TCAATAAAGA GGCTAATAGG GATGCAAGAG CAATCGCTTA CACTCAAAAT2040CTTAAAGGCA TCAAAAGGGA ATTGTCTGAT AAACTTGAAA AAATCAGCAA GGATTTGAAA2100GACTTTAGTA AATCTTTTGA TGAATTCAAA AATGGCAAAA ATAAGGATTT CAGCAAGGCA2160GAAGAAACGC TAAAAGCCCT TAAAGGCTCG GTGAAAGATT TAGGTATCAA TCCAGAATGG2220ATTTCAAAAG TTGAAAACCT TAATGCAGCT TTGAATGAAT TCAAAAATGG CAAAAATAAG2280GATTTCAGCA AGGTAACGCA AGCAAAAAGC GACCTTGAAA ATTCCGTTAA AGATGTGATC2340ATCAATCAAA AGGTAACGGA TAAAGTTGAC AATCTCAATC AAGCGGTATC AGTGGCTAAA2400GCAATGGGCG ATTTCAGTAG GGTAGAGCAA GTGTTAGCCG ATCTCAAAAA CTTCTCAAAG2460GAGCAATTGG CTCAACAAGC TCAAAAAAAT GAAGATTTCA ATACTGGAAA AAATTCTGAA2520CTATACCAAT CCGTTAAGAA TAGTGTAAAT AAAACCCTAG TCGGTAATGG GTTATCTGGA2580ATAGAGGCCA CAGCTCTCGC CAAAAATTTT TCGGATATCA AGAAAGAATT GAATGAGAAA2640TTTAAAAATT TCAATAACAA TAATAATGGA CTCAAAAACA GCACAGAACC CATTTATGCT2700AAAGTTAATA AAAAGAAAAC AGGACAAGTA GCTAGCCCTG AAGAACCCAT TTATACTCAA2760GTTGCTAAAA AGGTAAATGC AAAAATTGAC CGACTCAATC AAATAGCAAG TGGTTTGGGT2820GGTGTAGGGC AAGCAGCGGG CTTCCCTTTG AAAAGGCATG ATAAAGTTGA TGATCTCAGT2880AAGGTAGGGC TTTCAGCTAG CCCTGAACCC ATTTACGCTA CGATTGATGA TCTCGGCGGA2940CCTTTCCCTT TGAAAAGGCA TGATAAAGTT GATGATCTCA GTAAGGTAGG GCGATCAAGG3000AATCAAGAAT TGGCTCAGAA AATTGACAAT CTCAATCAAG CGGTATCAGA AGCTAAAGCA3060GGTTTTTTTG GCAACCTAGA GCAAACGATA GACAAGCTCA AAGATTCTAC AAAAAAGAAT3120GTTATGAATC TATATGTTGA AAGTGCAAAA AAAGTGCCTG CTAGTTTGTC AGCGAAATTG3180GACAATTATG CTATTAACAG CCACACACGC ATTAATAGCA ATATCCAAAA TGGAGCAATC3240AATGAAAAAG CGACCGGTAT GCTAACGCAA AAAAACCCTG AGTGGCTTAA GCTCGTGAAT3300GATAAGATAG TTGCGCATAA TGTGGGAAGC GTTTCTTTGT CAGAGTATGA TAAAATTGGC3360TTCAACCAGA AGAATATGAA AGATTATTCT GATTCGTTCA AGTTTTCCAC CAAGTTGAAC3420AATGCTGTAA AAGACATTAA GTCTGGCTTT ACGCACTTTT TAGCCAATGC ATTTTCTACA3480
GGATATTACT GCTTGGCGAG GGAAAATGCG GAGCATGGAA TCAAAAATGT TAATACAAAA3540GGTGGTTTCC AAAAATCTTA A 3561<210>27<211>70<212>DNA<213>OLIGO<400>27GCAGGGGTTT TATGTTGTGT CTTGAGCGGT GCTATGGGGA TTGTTGGGAT AATCTTTGTC60GCAATAGGAA 70<210>28<211>70<212>DNA<213>OLIGO<400>28TTATGTAAAT AAAGGCGGCT GTAAGGGCGT TTTACGAGAA ATAAATGGCT CAATGAAAAT60GGTTTGCTTG 70<210>29<211>70<212>DNA<213>OLIGO<400>29ATGTTTAGAA AACTAGCAAC CGCTGTATCG CTCATAGGCT TACTAACCTC TAACACTCTT60TATGCTAAAG 70<210>30<211>70<212>DNA<213>OLIGO
<400>30CAACAAAAGC GTGTATCAAT TAGTAGAAAT GGCAATAGGC GCTTACAATG GGGGAATGAA60ACACAACCCT 70<210>31<211>70<212>DNA<213>OLIGO<400>31TTTCATTCCG TTCTTTCAGC AATCGGTTAT ACTCTTGCCT TATTTGGATT TCATCATTGA60CATAAAGATT 70<210>32<211>70<212>DNA<213>OLIGO<400>32GCAGGTTGGA GAGTGATGTT TTTGAAACCA AAATAAGTGA ATGTGCCATC ATCAAAAATT60TCAGAGGGCA 70<210>33<211>70<212>DNA<213>OLIGO<400>33TTTACAGGTT TATCCAGTGA TTTGTTTTCT ATGATATTAA ATTTTTTGTT CTTCCTGATG60TTAATGATGG 70<210>34<211>70<212>DNA<213>OLIGO
<400>34CTTATAATAA AACCTAGCTT TTTTTATTAC ACTACTTATC TTTTGCTACT CGTTTCTCTC60AGCATAATAA 70<210>35<211>70<212>DNA<213>OLIGO<400>35CTACCACTAA AGATAAGGCT CAGGCTTTGC TTCAAATGGG TGTGTTTTCT TTAGATGAAG60AACAAAACAA 70<210>36<211>70<212>DNA<213>OLIGO<400>36TTGAAAAGTA TCTTCAAAAA ACTAGGTTCT GTCGCTCTTT ATTCTTTAGT TATTTATGGG60GGCTTAAACG 70<210>37<211>70<212>DNA<213>OLIGO<400>37GAGCGTTAAA CATGCCAATG ATAAGACACC AAGCAAATAG TAATTTAATT TTATAAAAAT60TCGTTTTCAT 70<210>38<211>70<212>DNA<213>OLIGO
<400>38GGACTCTATT GACAATCACG AAGTCAAAAA CTTCATCTTT GATAATATCG GGATTGACTT60CTTTGAAAGT 70<210>39<211>70<212>DNA<213>OLIGO<400>39ATCACTGACA GCTACAAGAA AGTTGTAGTA ACTTTAGGGC TAGTGGTAAC AACCAATCCT60TTAATGGCGG 70<210>40<211>70<212>DNA<213>OLIGO<400>40AGCTTTGAAT GAATTCAAAA ATGGCAAAAA TAAGGATTTC AGCAAGGTAA CGCAAGCAAA60AAGCGACCTT 70<210>41<211>22<212>DNA<213>引物<400>41ATGATACAAA GAGGATTGAG TA 22<210>42<211>21<212>DNA<213>引物<400>42
GCTAAAGAAA TAAGTGAAGC C 21<210>43<211>20<212>DNA<213>引物<400>43TGGATTGGTC TGACTTTGCT 20<210>44<211>20<212>DNA<213>引物<400>44ACAATGGGGG AATGAAACAC 20<210>45<211>20<212>DNA<213>引物<400>45TTCTAAAGAA GTGAGATAAG 20<210>46<211>18<212>DNA<213>引物<400>46AAATTCCCCA CTCTTTTC 18<210>47<211>18
<212>DNA<213>引物<400>47GCAAGTCTTG GGGAATAT 18<210>48<211>23<212>DNA<213>引物<400>48ACGCCTTTTT ATTTATCTCT GAC 23<210>49<211>20<212>DNA<213>引物<400>49TAAAAAATGC CTGCCCCATC 20<210>50<211>20<212>DNA<213>引物<400>50TTCGCTTACC GTCTGTTCGT 20<210>51<211>20<212>DNA<213>引物<400>51CCACCTAGCA ACTCACAGAG 20
<210>52<211>20<212>DNA<213>引物<400>52GATACCCTTA AATGACAAAC20<210>53<211>20<212>DNA<213>引物<400>53TTCATCAGCA AATCCCTAAC20<210>54<211>20<212>DNA<213>引物<400>54TACCAAAACC ATTACCAACC20<210>55<211>22<212>DNA<213>引物<400>55AACGAGATAA AAAAAGAACA AG 22<210>56<211>23<212>DNA
<213>引物<400>56CGCTAACCCT TTGAATGACA ACC 23<210>57<211>20<212>DNA<213>引物<400>57GGTATTGTCG CTTACTTTGT 20<210>58<211>20<212>DNA<213>引物<400>68GTTTCTTGCT GTCGTTTCTC 20<210>59<211>22<212>DNA<213>引物<400>19GTAATCAAGA TAAGTCAAAT GC 22<210>60<211>22<212>DNA<213>引物<400>60CGATGAATCT GGATATGTTG GT 22
<210>61<211>18<212>DNA<213>引物<400>61ATGCGTGAAG TCATAGGC 18<210>62<211>20<212>DNA<213>引物<400>62TAAACTTCTT TCACCCCACC 20<210>63<211>23<212>DNA<213>引物<400>63TGCCGCTGCA AGCGTATTAG AAT 23<210>64<211>21<212>DNA<213>引物<400>64GCTTCACATG TAATCGTATA A 21<210>65<211>18<212>DNA<213>引物
<400>65GCCAAACTTA GTGCTCAA 18<210>66<211>22<212>DNA<213>引物<400>66GCCACTACTA CCACCGACAT AC 22<210>67<211>20<212>DNA<213>引物<400>67GGGCGATTTC AGTAGGGTAG 20<210>68<211>22<212>DNA<213>引物<400>68GCCACTACTA CCACCGACAT AC 22<210>69<211>20<212>DNA<213>引物<400>69GGGCGATTTC AGTAGGGTAG 20<210>70
<211>22<212>DNA<213>引物<400>70GCCACTACTA CCACCGACAT AC 22<210>71<211>20<212>DNA<213>引物<400>71ACCAACGCCT CCAAGAATCC20<210>72<211>20<212>DNA<213>引物<400>72ATCAATACAA CTGAAGCAAC20<210>73<211>20<212>DNA<213>引物<400>73ATCGCTTGTT CTTTGTTGTC20<210>74<211>20<212>DNA<213>引物<400>74
TTGCCTTTTT CATTCCGTTC 20<210>75<211>20<212>DNA<213>引物<400>75TCTGCTCGGA CTTTATCTAC 20<210>76<211>20<212>DNA<213>引物<400>76ATGTTGCCAA GAATAATCCC 20<210>77<211>20<212>DNA<213>引物<400>77ATACCGCTCA TCATTTCAAC 20<210>78<211>20<212>DNA<213>引物<400>78TAAACTTCTT TCACCCCACC 20<210>79<211>18
<212>DNA<213>引物<400>79TTCAATCCAT TGCATTTG 18<210>80<211>21<212>DNA<213>引物<400>80TCTCCTTATC CACTAATAAC C 21<210>81<211>20<212>DNA<213>引物<400>81GTTTCATTCC CCCATTGTAA 20<210>82<211>21<212>DNA<213>引物<400>82AGGTGAAGAA GATAAGAGTG G 21<210>83<211>20<212>DNA<213>引物<400>83CAAATGAAGT AAAGGATAAG 20
<210>84<211>18<212>DNA<213>引物<400>84AGCGAAAGAA CTCCAACA 18<210>85<211>18<212>DNA<213>引物<400>85TGGCATTGTA GTCGCTGT 18<210>86<211>21<212>DNA<213>引物<400>86CAAAAAAGCC CTGAGGATAA C 21<210>87<211>20<212>DNA<213>引物<400>87GTTGAGGACA ATGTGAAAGG 20<210>88<211>20<212>DNA
<213>引物<400>88GGGGTGTGAT AGCCCTTGAT 20<210>89<211>20<212>DNA<213>引物<400>89TAAATCAAAT AGCCCATACC 20<210>90<211>20<212>DNA<213>引物<400>90CACTTACCAC TGAGCAAACT 20<210>91<211>20<212>DNA<213>引物<400>91AACATGCGAA AACTCTTCTC 20<210>92<211>20<212>DNA<213>引物<400>92TGCTTCCTAC TAAAACACGC 20
<210>93<211>23<212>DNA<213>引物<400>93ACGCCAAGCG TTCCGAGTTA AAA 23<210>94<211>22<212>DNA<213>引物<400>94GACGCTATTG GGCAGTTCAG AT 22<210>95<211>20<212>DNA<213>引物<400>95TTTTTTGCTC TTGTCTGTTT 20<210>96<211>20<212>DNA<213>引物<400>96CACAAGCCAA ACAAAAAACC 20<210>97<211>18<212>DNA
<213>引物<400>97AGGAATAGTA GCGGGTGC 18<210>98<211>20<212>DNA<213>引物<400>98GCTTGGTGTC TTATCATTGG 20<210>99<211>20<212>DNA<213>引物<400>99GAAAATGATG TCTTGACGAT 20<210>100<211>27<212>DNA<213>引物<400>100AAAAAAATAG AAGAACTTAA AGCAGAC 27<210>101<211>20<212>DNA<213>引物<400>101TTTTTCGCCA AACATTGCTC 20
<210>102<211>18<212>DNA<213>引物<400>102CTCGTTGTTT TCAGTTCG 18<210>103<211>21<212>DNA<213>引物<400>103TTACAAGAAT CACTGACAGC T 21<210>104<211>22<212>DNA<213>引物<400>104AGAGTGGTTT TTGTCTCCCC AT 22<210>105<211>20<212>DNA<213>引物<400>105GACAAAGAAA CGCTTCCCAC 20<210>106<211>22<212>DNA<213>引物
<400>106AGAGTGGTTT TTGTCTCCCC AT 22<210>107<211>20<212>DNA<213>引物<400>107GACAAAGAAA CGCTTCCCAC20<210>108<211>20<212>DNA<213>引物<400>108CTGACAAAGA AACGCTTCCC20<210>109<211>20<212>DNA<213>引物<400>109CTGACAAAGA AACGCTTCCC20<210>110<211>20<212>DNA<213>引物<400>110GCAAGAAATA CAAATTGGGG20<210>111
<211>22<212>DNA<213>引物<400>111CGAAAAAAAA CCAAGCAAAA AC 22<210>112<211>24<212>DNA<213>引物<400>112GATAGAGAAA GAGATGCCTT AATG24<210>113<211>20<212>DNA<213>引物<400>113CTTTCTTCAG TAAGCCTATC 20<210>114<211>20<212>DNA<213>引物<400>114CACAAGCCAA ACAAAAAACC 20<210>115<211>22<212>DNA<213>引物<400>115
AACAAAATAT ACCCAACCAA TC 22<210>116<211>20<212>DNA<213>引物<400>116AGTCTCGTTG TTTTCAGTTC20<210>117<211>11770<212>DNA<213>H.pylori<400>117TCTTGGCTGT GAGTAAGTAT TGACACCGCT ACGCATAAGC AGGTTTTGCA AAAATTGCAA60GAGTATCAAC AATACGCTTC GCAATTTTTA GCTCTAGTGC CTTTGAGCGC GAAAAAATCT120CAAAATTTAA ACGCGCTTTT AGAATGCATC AGCAAGCATT TAAGCCCTAG TGCATGGCTT180TTTGAAAAGG ATTTGATGAG CGATGAAAAA ATGCGCGATA TTTATAAGGA AATCATTAGG240GAGAGTTTGT TTGATTTTTT GAGCGATGAG ATCCCTTATG AAAGCGATGT GGTAATTGAT300AAATTTATAG AAGAAGAACG CATAGACAAG GTGTATGCGC ATATTATCGT AGAAAAAGAA360AGCCAAAAAA AAATCGTGAT AGGCAAAAAC GGGGTGAATA TCAAACGCAT CGGGACTAGC420GCGCGATTGA AAATGCAAGA AGTGGGCGAA AAAAAGGTTT TTTTAAACTT GCAAGTGATC480GCTCAAAAAT CATGGAGCAA GGAAGAAAAG AGCTTGCAAA AACTGGGTTA TATCTATCAA540AGGAATAGGG ATTGAAAAAA ATATTATCGG CTCTGTTAAT GGGGTTTGTG GGCTTGAATG600CTGATGATCG CTTGTTAGAA ATTGTGCATC TTTATCAAAA ACAAGGCTTG GAAGTGGTGG660GTCAAAAGCT GGATTCTTAT TTAGCGGATA AATCTTTTTG GGCAGAAGAG CTTCAAAATA720AGGACACGGA TTTTGGCTAT TATCAAAACA AGCAGTTTTT ATTTGTGGCG GATAAATCCA780AGCCTAGTTT AGAGTTTTAT GAAATAGACA ACAACATGCT TAAAAAAATC AACAGCTCTA840AAGCCCTTGT AGGCTCTAAA AAGGGCGATA AAACTTTAGA GGGCGATTTG GCAACGCCTA900TTGGAGTGTA TCGTATCACG CAGAAATTAG AGCGCTTGGA TCAATATTAT GGCGTTTTGG960CTTTTGTAAC GAATTACCCT AATTTGTATG ACACCTTGAA AAAACGCACC GGGCATGGCA1020TTTGGGTGCA TGGAATGCCT TTAAATGGCG ATCGGAATGA ATTGAACACC AAGGGCTGCA1080TTGCGATTGA AAACCCGCTT TTAAGCTCTT ATGATAAAGT GTTAAAAGGC GAAAAAGCGT1140TCCTTATCAC CTATGAAGAC AAGTTTTTCC CCAGCACTAA AGAAGAATTG AGCATGGTTT1200TAAGCTCCCT TTTTCAATGG AAAGAAGCCT GGGCTAGGGG CGATTTTGAA GGCTACATGC1260GTTTTTATAA CCCCAACTTC ACTCGCTATG ACGGCATGAA ATTCAACGCT TTTAAAGAGT1320ATAAAAAAAG GGTGTTTGCC GAAAAACGAA AAAAAGAATA TCGCTTTTCT TCTATCAATG1380TGATCCCTTA CCCCAATTCC CAGAACAAAC GCTTGTTTTA TGTGGCGTTT GACCAAGAAT1440TACAAAGCCT ACCAGCAAAA CAAGCTCTCT TATAGCTCTA ATTCTCAAAA AGAACTCTAT1500GTAGAGATTG AAAACAATCA AGTGTCTATT ATAATGGAAA AATAGGGTTT TGCTTTAATG1560
AGAGTCTAAA CGGATTTTTA ACCCATTCAG TCAAGCTTGA TACAAATTTT ATTAAAAACT1620ACAGAGCTTC CATGGCTTTT TCATAGCCAA ATTCTGCGGA TTTTTGCAAG AATTTTTTAG1680CCTTCTCCTT TTTCCTTCTA GTGCCAAGCC CTTCTTTATA AGCGATCCCA ATCCTATAAA1740GGATCTCCCC CATTTGTTGT TTGAGTAAAG TAATATTGCT TGAAATTTCA GCAATATCTT1800CCCAAAGAAG TGTCTTATCT TTGATTTGAG TGGATTTCTT AACAAGTATT CCAAACTTCT1860TTCCAAATTT TATAGGATTC GCGCTCAAAC CTGAAAGATC AATGAATCGC GATCCAATAA1920GCATACTAGT GAACGCAAGG TTTGGTAATC TAAAATTGTT AGCAAAATAA TTTGTGCTTT1980TATTAGAAAA CATGCGAACC ACATGCCTGT AAGTCTTAAG CGCTTGTTTC AAATCCTTTT2040TCATCCCTAA GCCTTGCGCT TGCATGCGTC CTAAAATAAG AGCGCCCCCT AACAACCCGC2100TCCCATACCC TTTGATAGCG TTTTGTGCAT AGAATTGCGC TTTTTTGTAA TCCACTTTCA2160CGCCCTTTCC CTCCAAATAC ATTTTGGCTA AATAAACGCT ACCAAGCCCA ATGCTGTATT2220CTTGCGCCAA TCTAAAATCT TTAAAGGCTT GCTTGTATTG GCCTTGATTA AAATGGTCTA2280AACCATTTTC AAAATAATAT GTCTTATGAT TTCGCGCAAT TTCATCCCAT TGATTTCGTG2340CCCTTTCATC CCAATCATCA CTTATGCTAA TATATTCACC AGCTATACAA AGCGAAAATG2400GCAATAATAA ACACAATAAA AGCAATAAAG GCTTTAAAAA AGAAAAAGTG GTGCGATAAA2460AATCTTTAAA CATGTCAGAT CCTTTTACAA TTTGAGCCAT TCTTTATAGC ATAATAGTAA2520CATGGAGATG GTTTTATATT TTTTGCTATA ATAAAATGAA TTTGTTTCAT AGTAACAAAT2580TGAGAATATA CCATTATGGA GGTAACACTA TGGCTGACAC AATCAATACA ACTGAAGCAA2640CCCATGAAAC AAAAAAGCCA AACGCTTTTG TAAATTTTTT CAAAAACAGT TTGACTGATA2700AGCGTTATGA TTCATTAGGT CTCATTGGAG CAGGGGTTTT ATGTTGTGTC TTGAGCGGTG2760CTATGGGGAT TGTTGGGATA ATCTTTGTCG CAATAGGAAT CTTTTTGTCT TTTTCTAATA2820TCAACTTAGT GAAATTAGTT GAAAAATTGT CCAAAAAGCA ATCTAAAGCG GCAACAACTG2880TCAATAACGA AACCCAAAAA TCTCAAGCAA CAAGCGTTAC CAACGAACCA ACTGAAGCTA2940AAGAGACTAA AGATTGAGGC AAAACAACGA TTTTGACTAA AAGAAAGAAT AAGAGAAAAT3000TTCAAAAGTT TAGGTGTTCT AGTGGTTTAT TACTCACTGC ATGACTCACA CAAGCGGTCT3060ATAAATTCTT TTTTTAGATT TAAGAATGGT TATTTGCAAT ATGAGTCTAG CTACGAGTTG3120GTGGGGTTGA TTAAATCCAT TGGCAAGGCT TTAAAAATGG AAACCAACTG AAAAAAAGGA3180AACTAATGTT TAGAAAACTA GCAACCGCTG TATCGCTCAT AGGCTTACTA ACCTCTAACA3240CTCTTTATGC TAAAGAAATA AGTGAAGCCG ATAAGGTCAT TAAGGCCACT AAAGAAACTA3300AAGAAACCAA GAAAGAAGCT AAACGACTCA AAAAAGAAGC CAGACGGCAC CAACAGATCC3360CTGATCATAA GAAACCTCAA TATGTTTCTG TTGATGACAC AAAAACTCAG GCGCTTTTTG3420ATATATACGA CACCTTGAAT GTGAATGACA AAAGCTTTGG GGATTGGTTT GGCAATAGTG3480CTTTGAAAGA CAAAACCTAT CTCTACGCTA TGGATCTATT GGATTACAAC AACTATCTAT3540CCATAGAAAA CCCCATTATC AAAACAAGAG CGGTGGGAAC CTATGCGGAT CTCATCATCA3600TCACAGGCTC ACTAGAACAA GTCAATGGGT ATTACAACAT TCTAAAAGCG CTCAACAAAC3660GAGACGCTAA GTTTGTCTTA AAAATCAATG AAAACCTGCC TTACGCCCAA GCGACTTTTT3720TGAGAGTGCC AAAAAGAAGC GATCCCAATG CCCATACGCT TGATAAGGGA GCGTCAATTG3780ATGAGAATAA GCTTTTTGAA CAACAGAAAC GCGCGTATTT CAAGTACGCC CACGATGTGA3840TCTGCAGACC CAATGATGAA GTGTGTTCGC CTTTGAGAGA TGCAATGGTA GCTATGCCCA3900CTAGCGATAG CTACTACTCA AAAACCTAAG ATCATTGCTC CTTATAGTTT GTATAGACTA3960AAGAGACAAA TAGCGCTAAT GAGGCTCAAC CATCGCCTTA GGCCACTCAA ACCGCTCCTG4020AAAACAGCAA AGAGAAGCTC ATAGAAGAGC TTATCGCTAC TCCCAACTCA TAGCCAATGA4080AGAAGAGAGG GGAAAAGAAA TCTTAGCAGA TAAAGAAAAA CAAGAGGCTG AATTGGCTAA4140ATACAAGCTC AAAGACTTAG AAAATCAAAA GAAACTAAAA GCTTTAGAAG CAGAGTTGAA4200AAAGAAAAAT GCTAAGAAAC CTAGAGTAGT GGAAGTGCCT ATTTCTCCTA GAACAAGTAA4260TCCTGATGAA ACAATGAGAG TTGTCAAGGA AAAAGAAAAC TATAATGGAT TGTTAGTGGA4320
TAAAGAAACC ACGATCAAAC GAAGCTATCA AGGGACTTTG ATTAGTGAAA ATTCTTACAG4380CAAAAAAACA CCTCTCAACC CTAATGACTT GAGAAGCTTA GAAGAAGAAA TTAAGAGCTA4440CTATATTAAG TCTAATGGCT TATGTTATAC TAATGGCATC AGTCTCTATG TCAAAATCAA4500AAATGACCCC TATAAAGAGG GAATGCTGTG TGGCTATGAG AGTATTCAAA ATCTGCTCTC4560ACCTCTAAAG GACAAGCTCA AGTACGACAA GCAAAAATTA CAAAAAGCGT TACTGAAAGA4620TTCAAAGTAA GTTAAAGTTT TGTTTGGGAA ATGGATTGGT CTCTTTTTAC TTCTTAATTC4680CTTAGCCTAT CCATGCCAAA AAGTAACCAT TATTTCAAGC AGTATGAGAA TCTTATCCAT4740ATTCACCAAA AAGGTTGCAA CAATGAAGTG GTGTGCAGAA CGCTCATCTC TATCGCCTTA4800TTGGAAAAGT TCTTTAGGGT TGAACAACAA GCGCGAAATT TCCCTAAAAG ACACTTCCTA4860TTCCATGTTT CACATCACTT GGACACCGCT AAAAAATTCT ACCCCACCTA TTCTAAAACG4920CTTCTCAAAT ACAAATTGCT CAATGATGTG GATTTTGCGA TTCATGGGCT AAAAGAATTT4980TGAAAGAAAA TTTTGATTAT TACAAACAAA AACACCCTAA TAAAAGCGTG TATCAATTAG5040TAGAAATGGC AGTAGGCGCT TACAATGGGG GAATGAAACA CAACCCTAAT GGCACTTATG5100TGAAAAAGTT CCGCTGTGTC TATTCTCAGG TGCGCTACAA CGAGTAGGGC ATACTAATTT5160TACAAGCAAT CTTGATGATA TACTTCTACG ATCTTATGAA TTTATAATAA TATCATTTAC5220ATCATTATCT TTTAATTTTT CTAAAATATA TTTCGCTTCT TTTTGTTAAG ATATTTTGTC5280TTTTTATTTT TTGGAATAGG CTTTTTGCTT TCCTTATAAG GACATATAGC GTAAAAATTT5340TTCCAATTCA TACGATTTTG GCATAGGTGC TGTAAAAATG GATTGATGAA TTTTAACCTA5400GTGCATGCAA ACAATCCACT AGAGCGATGG TTGGCAAATC AAAGAACACA CAAATCACAG5460TTCGCTTGAA CCCACAGGCA CTAAAGATTT ATCATAACTC AATTCGCCTC TGGTTTTAGC5520GGCTTGCAAA TCATCATAAA AAGTCGTCTG ATCAGGCACT TTCCCCAATT TGTATTTCTT5580GCTCAAACTT GGACTCACCT TAATGAGTTC ATCGGTGAAG AAAGGATCAT CGTAGTAAAG5640CGCCTTGTGG CACTTGATGG GTTTGAGCGT GTTTTCTAGA ATGATAAGCT CATCGCCCAT5700AGTCATCAAT TCATCAGGGG TCATCAAAAA GCGCTCTTTG TTGCTGATAG AAGTGTTGGT5760CTTACCGGTA TTATCATCAA TGCTTCGGCT CACATCTTGC CTTGTGTATT TCCCTAACAC5820CTTAGAAAGT TTTTCAAAAT GTTCATAGTA GTTATCGTTG TTAATCCCAT AATACATATT5880CAAAGAAAGG TTGTCTAAAA TAGTCTTAGC ACCATTCCTA CCATAACCAA GTGGGGGGTC5940ATTCTCTAGT TGCGCCTTAC TTTGAAACAC AAACGCAGGG CGCATGTTGT ATTCTGCCAT6000GATCCCTACC GCTTTAACAA AGGTCTCTAA ATAGCCACAA AGCGTAAATT CGTCCATGAG6060CATTAAACAA CTTCTTTTGC ATTGTGGATC ATGGATTGGC AGAATAAGGT TGCTATAAAT6120CATCACATTG AAAAACAGCT CTAATATCGG CCCAACAATA GTGCTTTCTT TAGGATTAGC6180GATCACGCCA ATACTCACTT CATCAATCCT TAAACGCCTG AAATCAAAAT CATTAGCGCT6240CGTGAAATTT CTGATCATGG CGTTATTGTA GGGGGCAAAC GCTGAAGTAT AGACCCCTTG6300AACAGAGCTG TAAGTTTCTT TAGCGCCGCC CATTGTCTTG AAATTATTCC ACATGTTTCT6360AGTGGCAGGA CTAAGCACTC TTATATTATC GCCACTCTTA TCTTCTTCAC CTCCAAAAAA6420TTCCATCAGA GACACGACTT TTTCCATGTT TGTGTCTTCA TCAATCAAGT TGATACCGCT6480TGCCATAGAA CCTATGAAAA ACATCGTGGG GGTTTCAGGC ATGATGATTT TTTTTCTTTT6540GACAAACTCA AGTCCCTTTT TAGTCCACAT GAGATCTCTA TAAATATTGC AATTGATGAC6600AAAAAGATTT CGCGCTTGAT TGCTAAAAAA AGGATCTTTT TCATTAGGTC TTTCAGGGAA6660CACGAGCTTT GCCAATCCAA AGATTTGAGT GGAAAAATCC CCCCCACTAG CCACCATCCC6720ATGACCTTTT AGGCGTGTGT CAATTTGAGA GAGTATGTCT TCAGTTAAAA CCACATCATT6780ACCAAAATCC ACATAAGCGA AAGGATTAAA TCGGTGTGTT TTTAAGGAGA AAGGTTCATA6840GATATACACT TTTTGGTTGA AGCAGTTTTT CTCTGATTTT TCCGCAAGTT TCCATAGTGT6900CAGCCTTAGG GTCAAACACA ACGATATTTT GAGGATAATT GATCATATTA GGCATGATGA6960AACCCACCCC CTTACCGCTT CTGGTAGGAG CAGATCAAGC CAATGAACGC CTGCCCTGCG7020TAAGCGATAA AATCCCCCAA ACCACGCCTG CCAACAATAA CTTCTCGTTT GTCAAAGGCA7080
GCGTTTTTTA TTATTGGGTG TGATAAGTTT AGCTTTGATC ATTTTTTCTT CGCTCTCCCA7140ACTCGCGCTA CCAAAGAGAT CATCAACTTT TTTATTCGCT CCTATGTCTC TAGTCCGAGT7200TAAGTATTTT AAGAACCACA CAAAGAAGGT AATGAATATA TAAGAGCATA AAATAGAACT7260ATAGACGACT AAGGATATAT TGAACCCATA AATCTTGAGT GAACTAAAAC AAAGCGCCTT7320AAAATAGTAT GCAGGAAAAT CTTGTATCGC ATAGATCCAT ATGTCAAAAA GATCATCATC7380ACTCTCAGGC AATTTATTAT CATCATAAAA ATTACCTAAT AGGATCAAGA AAAATAACCC7440TGATGTGATA AAAGAATAGG ATAGTAATAG TGTAGCTTGT AACCAAAAGC CAAAGGTTTT7500AACTCTTTCT TTCAGCCCCT CTATAATGAT TTCTTTAAAG TTTTTTTTCT TTTGAGGTTG7560GTTAGCTTTA TCTTTAAAAG CTTTTTTTTG AGCTTTTATG AATTTGTAGA GAAAAAATAA7620CGCTATTACC CCTATGAAAC TAAAAATAAC AACCAACTTA TAATCCTCTA TGAAATATAA7680GGTGTTATAC AACAAGTCTT CCATTGTACC TACTACCTAT GTTTGATATA AAATTCATCA7740CACTGTTTGT GGTGGTTGAT ATGGACAATC ATATCAATTA AATCTTTAAA GCCCTCAATG7800AGGCTTTCAA ACTTGATATT CCTTGCTGCG TTATTAGATG AACTCATGTT GGCTAAACGG7860ATAAACGCTT CTTCACTGCT CCCTGCATGT AGAGTGGTTA GCGTGCCTTT ATGACCGCTA7920CAAAGCACAT TATAAAAATC ATACGCCTCA CTGCTTCTGA GTTCTCCTAA AATGATTCTA7980TCAGGCCGCA TTCTCAAACA TGACTTTAAA CAATCAGCAG AGGTGATATT CCCACCAAAA8040AAAAGCTGTG TGTAGTTCTT GTGGTGTTTG AATACAATCT CTTCGGTGTC TTCAATGGAT8100ATGATCCTTT CTTCTTTGGG GATAAACTCC ATGATGCTTT TGATATAAGT CGTTTTACCG8160CTTCCTGTGC CACCACAAAC AATCACATTC TTACCAATAG CAATACCATC TTTAATCGCG8220CTGATCGCTT GTTCTTTGTT ATCTAGTAGA TTATAAAAAC CCTGCTCTTC AAAGAAGCTA8280TGAGGATAGG TTGTTTTGCT AGGTATCCTT ATGGATATGG AAATGGTTTC ATCATTAACT8340GTAACAGGGG AAAGGACAAT CTGCACCCTT TCACCATTCG CTAAATTGCT GCTCAAAATA8400GGATTTTCAT AGTTGTCTAT TGTTTTTTTC TTAAAACTCG CACAACACCG AGCAAAATGC8460ATTAAACGAG ACAGACTAAA GGCTTTCTTG TCTCTCACAT CAAATGGTTG CCATTCGCCA8520TTATTTTTTA AAACCCATAC AACCTTGTTC CCATTGTAAC AAATCTCAGT GATATTTTCC8580ATTTTTAAAA AATCACCAAA AAGTTCTTCA GTAGCATGCC TTAGAGGATT TAATGCCGCT8640TCTTTTAAAG CTCTTTCCAC TTCTAGAAAT TTTTTATCTT CTGCACTCAA TCTGTCTTCA8700GTCATGTTCT TATTCCAAAT TTAATTTTAA TTGGGTTATC AATTTAGCTC TTTCTAAAGA8760AGTGAGATAA GTCTTATTTT TGCTCAAAAT AATATATTTA GTCCCTATAT CTTGGACAAT8820CTCATTGAGC TTCCTTCCAA AATCATCTAT CCCTAGAATC TGAAACGCCA CGCCCATCAT8880ATTGCCATCT ATGAATGGCG GTAAATACTC TCTTGGCAGA TAAGAAGCCA CTAGGTCTGA8940AATTTTTGCC ACTTCATCAC ACATAAAATT ATTGAAGTTG TCAGAAATTT CTGCAACAAA9000CTCCCCAAGC GTTAGTTCAT TTTTGATCGT GCTTTCTGCA AAAGTCTTTT CTTGTTTGCA9060ACGATTGTAA CTGGCTAACA CAAGGTTGTT CAAATAGTTG TAATAAGCAT CAAAACTAAT9120AAAATTCTTT TGCATATTGA TTCTTAACTC TTGAACTCTT CTCAAAAAAT CTTGCGGATC9180GTTGCTATCT TTTAAAGAAA TGGCGTAATT TTGAATAAAC TTATCCTTTA CTTCATTTGC9240ATTTCCCATA AGAGATCTGT TGCTATCCAA GATCTTTTGT CTTTCTGCTT CATTGAAACC9300GAGTTCCATT GCTGTTTCCT TTCATTGTTT TTGCTTGGTT TTTTTTCCGC CACTACATCC9360TCTAGAAGAG CCTGTTGTTG GCTTTGTCTT TTATTTTGTA AAGAACCAAG TTTGGTAACA9420CAGATATATT TTATCCTTTC TTTTGAATTA ATTGCCACCT TTGGGGCTTG TGGTGATTTC9480TTCATGCTCT CTAGACAAAG TTTTAGTGCT TTGTTTGATA ATTTCATCCA CCACAGATTT9540GTTGGTAATT TTAACATCAT ACACGCCACT AAAATCAATG TCGTCCATTG TGAGAATCTT9600AATACTATCG CCCTCATTTT TGTAAAAACT TGGGGGGATA TTCATCAGTT GCCCTAGAAT9660TTGATTAGAC ATCTGAGCTG AACTTTGCAT ACTACCATTG ATCGCTTGAC CCAAAGCGTA9720ATTAAATTCA GGTATCCTTT CACTTCTACC TTTGCCAAGG CCTATGAGTT TATCTAGAGC9780TATGATAGGC GCAGTTTGCA AGAAGCTATT AACCACGCTT GCTATCACAG CAAAGCCTAT9840
GCGCTTCATG AAGTGGTTAT TCACATAGCC ATCTACCCCT GCTTCACCCA GCATACCTGC9900TGCTTGAGCG TTTGCTAGAG GTATTATCAC ACCATCAGGC GTAATGGCTT TAGTAAAGAC9960TATCATTAAG CGTGTCATAA TGGGTGTGCC ACCTTTCACG CTTTGATAAT TCCCATACAC10020CTTAGTGCCT TTGTCTAGTA AGATCATAGT GCCGTTCATG TTCCATACAT CTTTGGCTAC10080AACCCCACTC ACTATACCTG TGAGAGTGGC ATCTACTTTA GAAGTCAGAG TGATTTCAAT10140AGGGGTGTAT TGCGCTAAAA CAAATGTGGG ATCACTCTTG CCTATAAAGG CCTGTTTGAC10200AGGGCTTGTT TCATCTTGTG TTTCTGTTTT CTTTTTGTCA TCAATGGGAT TACCATTTTC10260ATCTACAAAT TCCCCACTCT TTTCTTTTTT GTTCTTAAGA GCGATTTCAC TGATATTCTT10320GGCAGTTTCT GCGACATCTT TGTCTATTTT ATTGCTTTCT ACTCTAGCTT CTGCTGTGGA10380TTTTTTACTT TTTTCTTTAT CGTCATCTTT TTTATTGCCA CCTAAAGCCT TAGCTAATTT10440AGCTTCTAAA TTCTTATCCT TAATGGTTTT TTCTGTTTCG TATTGCTTGG TAATGTCAGG10500CTCTATGGAA GCATAAATAG GATTATCGCT AGCTATTTTG TCCGCATCAA CATTAGTGGC10560GTTAATAGTG GCAATATCGC CGCCATTTTT GAAATCCATT GGCAACAATG GATAACCTTT10620AGCCGCCATG TTATCAAAGG TTTTGCGGTT TCTTAGATCG CTATAAATCA GATCCACTTC10680ATCAGCTTGT CGTTTAATTC CTAGAATTAG CGCCCTTTCA CTATCGCTCA AGCCCTCTAA10740ACATTGCTCT ATGGCTTCTT GATCAGTAGG GTCATCTAAG TTATCCAAGC ACTCGCTTGC10800TTGATGCAAT CTTTCTGTTT TACTCAATTG ATTTTGTTTG CTTTGTGTGC TTTTATTTTG10860GATTTCTTGG ATCAAATCGC TATAAAGGTT TTGACATTTC CTTTTTTCTT CATCGGTCCT10920AGCCGTTTTC AAACAATCCA AGACAGCCTT TTCTCTAGCT TCTTGCAGGT ATTTGAGCTT10980CTCTTCATCG CTCAAACCAT CCAAACACTT CATAATGGCC GCTCTGTCGT TAGGATCGGC11040GTTTTTCAAG CAWTCTTTGA TCGCTTTATC YTTTTGYTGG AGTTCTTTCG CTAAAAATTT11100CCTCGCTTCA GGGGTGAGTA ATTTCTCGCA TTCTTGTTTC TCTTTTTCAT TCCTAGCTTT11160TGATACGCAG TCCAAATAAG CCTTGACGCT CTTCTTAACT TCTTGCTCTA AGAGTTTTCT11220CGCTTCAGGG GTGAGTAATT TCTCGCAAGC TCTCCTTTCT TCTTCATTTC TAGCTTGAGA11280GAGGCAGTCT TTATAAGCTT TAAGACTCTC TTTAGCTTCT TCTAAAAGTT TTTTCGCTTC11340AGGCGTGAGT AATTTCTCGC AAGCTCTCCT TTCTTCTTCA TTTCTAGCTT GAGAGAGGCA11400GTCTTTATAA GCTTTAAGAC TCTCTTTAGC TTCTTCTAAA AGTTTTTTCG CTTCAGGCGT11460GAGTAATTTC TCGCATTCTT TTTCTCTTTT TTCATTCCTA GCTCTTGATA CGCAATCCAA11520ATAAGCCTTA ACGCTCTCTT TGGCTAAAAC CTTTTTCTGC AAGTCTTTAG GGAGATCTTT11580GACACACCTC TTTTTCTCAA GCTTCGGTTT TAGCACTTTT TCAAACAATC CTAGCGCTTN11640GTTGCTCCTA AAAGCTTTTT CGCTTCAGGG CGTGAGCAAT TTCTCCCATN CTTTTTTCTC11700AGCTCCAGTT TNTGNCCTGG AGANCCGCAA TCCTAAATAN GCTTTAAACN CTTCCTTTNA11760GCTTTCNTCT 11770<210>118<211>25238<212>DNA<213>H.pylori<400>118TTTTTCGCTT GAGGCGTGAG TAACTTCTCG CATTCTTTTT TCTCAGCTTC AGTTTTGGCT60TGAGATACGC AATCTAAATA AGCTTTAACG CTTTTTTTAG CTTCTTCTTC TAAAAGCTTT120TTCGCTTCAG GCGTGAGTAA CTTCTCGCAT TCTTTTTTCT CAGCTTCAGT TTTGGCTTGA180GATACGCAGT CTTTATAAGC TTTAAGACTC TCTTTAGCTA AAATATCGCT TTGTAAGTCT240
TTAGGGAGAT CTTTCAAACA CTTTTTTCGT TCTCCATCAG TTTTAGCGTT TTTCAAACAA300TCTAGAACCT GTTGTTCTAA CTTTTTTTTC GCTTCAGGCG TGAGTAACTT CTCGCATTCT360TTTTTCTCTT TTTCATTCCT AGCTTTTGAT ACGCAATCCT TGTAAGCCTT GACGCTCATA420TCAGCTAGTA GTTCTTTTTG CAAGTCTTGG GGAATATTTT TCAAGCACTC GTTTCGTTCT480TCATCGGTTT TAGCGTTTTT CAAACAATCT AGCGCTTGCT GTTTCAATCT TTCTATAGCT540TCTTTAGACA AGCCTTTCAA GCATTCGTTT TTCTCAGCTT CTGTTTTAGC GTTTTTGATA600CAATCCTTAT ACTCTTGAAG CTCTTTTTGA ATCCCTAATT CCTTACGGAA TTTCTCTCTA660ATCTCAGGGT CATTTATGAG TTTTAGGCAC TCGTTTCGTT CTTCATCGGT TTTAGCGTTT720TTCAAACAAT CTAGTGCCAC TTGAACTTTT TGTTGGTTCA GTAAGCTTTT TTTCAGGTTT780TCATCTTTGA TTAAATCTAA ACACTTGATC CTTTCTTCTT CAGTTTTGGC ATTTTTGATG840CAGTCATTAT AAGCCTCTAA AGTCCTTTTC ATTTGATCTT GAAGTTTTTT GTCTTTGATA900AGCTTCAAAC ATTCTTCATA GTTGCCACCA TTACTAATAC ATTCATAAAA GGCTCTCAAT960GGGTTTTTGT CCTCAATTTC TGCAATATTC AAATAGTTGT ATAAGGTTCT ATTGGGATCG1020TCATTGAAGA AAAGATTCTT ATCGATCATA TTGCCTTTTT CATTCCGTTC TTTCAGCAAT1080CGGTTATACT CTTGCCTTAT TTGGATTTCA TCATTGACAT AAAGATTCCT GTCTTTGCTA1140AAACGAGAGC TTTTATCTTC CAAAGGTATG AAGTAGTGAA AAATGCTTCT AGAAAATAAA1200ATAATCACGA TAAGAACAGC GCTACAATAC CACCAATAAT GTATTTCTTT TTGCTTCCTT1260TGATAATCTC TTGATCGTTA GAATCGTTAA TTATTTCTTC TGACTCGTAT TCATCAAAAT1320TGGTTTTAGT TTCTGTGGGG TTGTCAAGAT AATCTGAGCT TTCTTTGGAT TCTTTTGAAG1380AATCTTCAAA ATGATTGGCT TCTGTTGCTT CTTCATTGGA TAAATCTTGG GGTGAATGTT1440GTTGGGTTTT TTTAGAAGCT TCATCATCAA TTAAGTCCAT TTGAGTTTGG GTTTCTTCTG1500ACTCGTATTC ATCAAAATTG GTTTTAGTTT CTGTGGGGTT GTCAAGATGA TGATCTGAGC1560TTTCTTTGGA TTCTTTTGAA GAATCTTCAA AATGATTGGC TTCTGTTGCT TCTTCATTGG1620ATAAATCTTG GGGTGAATGT TGTTGGGTTT TTTTAGAAGT TTCATCATCA ATTAAGTCCG1680TTTGAGTTTC TTCTTGATAC CCATTATTTT CTTGATTGTC TTCTTCGTTC GGTTCTTGGA1740TTTCTTCATC TAAATTCTTT TGCTGAGTGA AAGGTCTTTT ATTATCAACT AATTTTCTGG1800CTTTTTTGAA TAACTTGTCT GCTAGACTGC CATTGCTAGA TTCTGAAGTT TCATTACCTC1860CAGAATCCAT TTGAGTTTGG GTTTCTTCTA GCTCGTATTC ATCAAAATTG GTTTTAGTTT1920CTGTGGGGTT GTCAAGATGA TGATCTGAGC TTTCTTTGGA TTCTTTTGAA GAATCTTCAA1980AGGTGATTGG CTTCTGTTGC TTCTTCATTG GATAAATCTT GGGGTGAATG TTGTTGGGTT2040TTTTTAGAAG TTTCAAATTT ATCGTTTTCT TCATTCATGT CTTAACGCCT TTTTATTTAT2100CTCTGACAAG AGGGAGCTTT TTAATCACAC GCTCCAATTC ACCATAGTTT TTGATATTGT2160AATTTTTTGT CAATGGATTT TTCCCATAGC CTTTATTTAT TACTGTTACA AGGGCTTTGT2220CTTTAATGAG CTTAAACTTT TCTGCAATTT CATTAACTCT ATACCATCTC AATCCTGAAT2280TGGTCATGTT AGGATCAATA GCAGCATCAG TCATGCTTAA TTTCCCATCA GGCTGAACCA2340CAAAAATAGC AGGTTGGAGA GTGATGTTTT TGAAACCAAA ATAAGTGAAT GTGCCATCAT2400CAAAAATTTC AGAGGGCATA ATATGCTTAG AGCGTTTTTC AGGCGCTTGG TAATAATTGT2460AGTTTCTAGG CACAGGGTTT CTTTTTAATG CATTATGCAC ATATTGAGTC TCTAGCGCCT2520TTGCTTGATC TAATATAATT TTTTGTTTTT CTTCTCTTAT TTTTTCTTTG TTGATAATCT2580GTTCATTGCT CGCCATCATC ACTCTATTGA TATAGGCTGT GGTATTAAGA TTTTCTTGCT2640TGATCAATTC TCTCTGCCTC TTTGCTTCTT CTCTCTTTTT TAACTCCTCT TCAATAACAC2700TAGAGACTTC GTGTCTTTGT GGGTATTCTA ATTTGACTGT TAGATACGCC GAAGCAAAAT2760TGTCTTTTTG AGCTATTCTC AAAATGAATT GATACAAGGC TTTATTAGTC CGCACAACAA2820GATTAGTTCT CCAAGCGCTA TCGCTAGGAG ATAATTCTAT GGAGTTATCC TCAGGGCTTT2880TTTGAGACTT ATCTGTCTTA ATATTGATTT TATCCTTGGC TCTTTGTTTC ACTGCCTCTT2940CAGCTTGTTT TTTGTTGAGT TCTTCAATTT GTTTGAGCGC ATTAGCTTGA GCCTGTTCTT3000
GCATGTCCTC TAGTCGTTCC ATTTGATCTA ATTCATTTTC TCGTTGTTGC TTGATAAATT3060CGCTAAGATT TTTGTTATTG CTCAAATTTT GTGGGTTACT CATAGCGTTA GTGAGATTTT3120CTAAATTGGC TCTATTTTTT TGCACGCTCT TCCTTTCTTT TTTCTCTTTT ATCTTTTTGC3180GCTTTTTGCG CCTGTTCTTT AGCTTCTTTT TCTTTTTCTA GAGCTTTTTT TTGTTCTTCT3240AATTCTTTAG GGTCAGGCGC ATCTACGATA AGTTTTTTTG TTTTTAAAAA TTCTTGATAA3300TCTCTTGTCA TTAGGGCAAA ATTCACTGCT TCTTTTTCAA ACATGAGATT ACTTTTTACC3360GATTTAGGTT GAATGAATAT ATGATTAGAA TTAGGCACAA TACTCCAACC TTTATTGAAA3420CCTGTTGTGA TGTAAGAAAT AGTTTCATCT TTTTCAAGTT GGATCACAGT AACATTGTCT3480AATGAAGTCC AAATCGTAAT AGGTTTTTCA TCTCCCAAAT AAGCAATCTT CTTATTCACC3540ACTTTCACCC TACCACGATT AAAATTTTTA ATGTCAGGTG CTGCTGCTTC TATTACGCTA3600GATAAAAATA AATAACCAAG ACAGAAACAG CCAACAATTT TTTTAAAAAA TGCCTGCTCC3660ATCAACAATT CCTCTTAAAA AATATTTATA ATTGTAACAC AAGAATAAAG TGGCTTATCC3720TTTAAACATA GATCCACCAA CTCTTCCCAC CATTAAGGCA TCTCTTTCTC TATCCATTCT3780GCTACCATCC ATAGATACAG AGCTGAAAAT GGTATTAACG ATCGCATTGA CACTCTCAAT3840AATCGTCTCT ATGAAACCTT TTAAAATAAA TAGAGTGATC AAACTCGCAA TAGTTGAATC3900GATACCCAGT CCCCCTTGAT TGATGTTTTG TATAGCAACA GAGGGCGTTA TGGTCAAATT3960GAATGCCAAT AATGCCAAAA CACCAAAGAG CAAAATAGGA ATAGCTAGAA CCAAGATAAA4020CAAATTATTA ATAAACCATA TCAATATGTT TTTCATAGAA TCTTTGAACC AATCTAGCAC4080GATCAAACAC ACCGCAATAG GGAACACAAT ACCGCTAAAA GCCCCCGCTA TCAAAGGTTT4140CACAACAACA GTGCTTATTC TCATAATGAT AGTTACTTGC ATAAAAATAG CGAATAAAGA4200AATACCTGCC ACTTGCAAGC CTTGTAACAA ACTAGGAGCG TTGTTGCCAA CATTAGAAAA4260AACAGCTCTT ACTGCAGGTA AAATTGAATT AACAGTGTAA TTTAACACTA AATCTAAAGC4320CCAAAAAAGA ACCTGATTCA GCCCAAAAGC GCCTGCTAAA CCAAAATAAA TGGAATAAGA4380GATCTTATAA AGATAACCAA AACCTGCATA GGCGATAAAA GAGACCATCG CCATTTTGAA4440TAATGTCCCC CCAACAAATC GCATGTATTC AAAACCTATG TTGATTTGAA TGCCATTTGA4500AATATCCCTC AATTTCATAA AAGAAATAAA AACAAAAATC CCAAAAACAA TATAAATCAG4560GTAAGAAACA AAATCAAGAT ATGACCCTTG CCCTATGATA ATATCAGGCA GATTGACTAC4620ACCATATGCA GGAAGAACAG CAGCATCCGC TCCTTTGACA ACAGATTCCA GTGCGGCAAA4680AGCAAGCATG GCTGTAGCGT TGTTTTGCAG TTTATTTGCA GTGAATTCTT TACCAATCAG4740CCAAATTAAA CCATCAGCTG TAATATTCGT AGAAGCTTGA GCGGTTATTG AGCTAATTAT4800TTGTAAAAGA ACATTGCCTT TCACATTGTC CTCAACACCG CCTTTGGTAA AATTCTGAAT4860CTTGTTGCTC AAATTGTCTA GCATGCTATC CATGGATACA TCTATGTTTT TATTTATCAA4920TGCATTCCAA CCAAAATTAC AGCCACTTGA TGGCGTTAGA GCAAAACCTT GTATAGCTGT4980AGCAACACTT GCCCACGCTG ATCCCCATAA AGGACCTAAA GTATTCAAAT ATTTCACTAG5040ATTGCTAGCG GCTAAACTTG TGGCTAATGG TGTGCTAAGC GCTTCGATCA CACCCTGCCC5100TGAACTCTTT CCACTCCCTG ATGCACCAAT AGATGTATAG CCTGTCAAAA TATCTGCAAA5160ACTTTTGTTG GTTAAAACAT AACTCGGTAT CAGCATGCAA TAAACTGTTT GGTAAATTTT5220GAGATTTGAA ACAATGGGCA AATCAATAGC TTTCAACCAA TTAGGAACAA TGAGAAAAAA5280ACTTATGATC GTTATTAAAA AAGTCCTTTT AATATTAAAC ATGCTCTTAT TTCTTTAATG5340CCTTATTTTT TGATTCCACT TCTTTAATCT TTCTCAAAAT TTCGCTTACC GTCTGTTCGT5400TTTGTAAATC TGTGATTTGA ATGTCAAACA CTTTGAAGCC AAAAGGATTG ATGATAAGAT5460TTTCTTGAGA AGAGTTACCT CTAGCAAAAT CAGTAATATA TTCATAATTT TCCATTGTAT5520CGGGTGTGAT TTTGATGGTA ATGAAAAATG TTAATCTCGT TAAGGGACTA TTTTTGACTT5580TTTCTCTTTG TATGTTAGAG CTAATGATAG CTTCTGCTCG GACTTTATCT ACAAATTGTC5640TGATATTTTC ATTGAACATC CTCATTGCTT GGGTTTGGAA ACTCACATCG CAATACTGCA5700TTAGTTGATC CTTGCGATCC CTCAAAGAAT TTTTGCTATA ACCAAACAGC AATGAAACAA5760
ATTTTGAAGT TGCACTATCC ACAACAGCTT CAGAATTGAC AATTTGCCTA GCATCGGAGC5820GTTTGACAAT TTTAAATTCT CCTGTGTATC GATCAATGCC ATAAACAAAT ATATCTGTTT5880TCTTCAAAGG CATCATCATC ACAATACTTG CTACAGCGGC GATATTGAGT GCCATAGAGA5940GATAAAAAGC CCTTTTGAAA GTCCTATTGG CCTTATTGAG TTTTGCCAAT CTATCAAGGG6000CTATCACACC CCCAACTAAA TTTTCATCAA TCAAGACTTC CTCGTTTTTT TTTCCTAACA6060TGCGATAGCT TTATTGTTTA GTTATTGGTT TGTTGGTTGT AAAACTTGAG AAATTTTTTC6120ATTAAGAACC CCTTAAAATA ATCTCAATTA TAGCAAAATT TCTGTAAAAA TATGTATTTA6180ATTTAAGGTA AGAGTAATGA GTAAGCAGTC GTAACCCAGT AATGAAATTT TAGAAGCGCC6240TAATTCAAAG AGGTAAGACA ATCACAACTC AGAGAGAGAA TGGTATAATA AGAAAACAAT6300TTCTCAATTT AATTCTTGAA AGGAGTTTCA TGAACGATAC AACAGAGCAT CATGGATCCA6360ATCCGCTAAA CGCCCCATCA CCTAGCAACT CACAGAGCAA TGATCTTTTA AATTTGCTAG6420ACTCGTTATA TCCCAAAGGG AGTTTAGGGG AGCAAAGATT TCACGAAGCT CTAAAGAATC6480AAGAAGAGTT GAAAAATATC CTAATAGAAA TAGAAAAGCT ACCGCAAGAA AAAAGGTATG6540AACTTCTGAT GCAGATAGGG CAAGCCAAAC AGAGAATAAT GGAAGCATAC GCTCATTCAT6600TCTTGGGATA TATAGGGGGA CTAGAGCATC TGTTAGGATT ATGTATGGGT GGGATATTTG6660TTCTGTTTGC AATCTATTTT GTATTTTTAA GAACTAGCAA AAATACGGAT CTGGTGGAAA6720GTCTAAAAAC AAAACTAAAA CTTCAGTATT TTTACTATGC CTTTGGTGCA GGTGCGGTTT6780TGTTTTTTGG ATTAGAAACA ATTAGATCTA TTTATGAACT ATATATATTA GGAATTGGTA6840GCACTAACGA CAAGGTGCTC TTTGTTTTGA AAAACATTTG CTTCATAGGT ATGGGCTATT6900TGATTTATAA AGTTATTAAG GTTATTGGTA TAAAAAATTT TATCAATGGT CTTTTCATTT6960CAAAGAAACA AGAATAATAA GGAGAAACAA CAATGAAACT GAGAGCAAGT GTTTTAATCG7020GTGCGACAAT TCTGTGCTTA ATTTTAAGTG CATGCAGTAA TTATGCGAAA AAAGTGGTGA7080AACAAAAGAA CCATGTTTAT ACGCCTGTGT ATAATGAACT GATAGAGAAG TATAGTGAGA7140TACCCTTAAA TGACAAACTC AAAGACACAC CATTCATGGT GCAAGTGAAG TTGCCAAATT7200ACAAGGACTA TTTGTTGGAT AATAAACAAG TTGTATTAAC TTTCAAACTT GTTCATCATT7260CTAAAAAGAT TACGCTCATA GGCGATGCCA ATAAGATACT TCAATACAAG AATTACTTCC7320AAGCTAATGG GAGCAAGGAT CTGCATTGAT TTTTACTTGC AGCCTACTTT GAATCAAAAG7380GGTGTGGTGA TGATAGCGAG TAACTACAAT GATAATCCCA ACAACAAAGA ACAACCACAG7440ACCTTTGATG TGTTGCAAGG AAGTCAGCCA ATGCTAGGAG CTAACACAAA AAACTTGCAT7500GGCTATGATG TGAGTGGAGC AAACAACAAG CAAGTGATCA ATGAAGTGGC AAGAGAAAAA7560GCTCAGCTAG AAAAAATCAA TCAGTATTAC AAAACGCTCT TACAAGACAA GGAACAAGAA7620TATACCACTA GGAAAAATAA CCAACGAGAA ATTTTAGAAA CATTAAGTAA TCGTGCAGGT7680TATCAAATGA GGCAGAATGT GATTAGTTCT GAGATTTTTA AGAATGGCAA CTTGAACATG7740CAAGCCAAAG AAGAAGAAGT TAGGGAGAAG CTACAAGAAG AAAGAGAGAA TGAATACTTG7800CGCAATCAAA TCAGAAGTTT GCTCAGTGGT AAGTGATTGG AAGAAAGGGA GAGAGTAGAT7860TTTTCTAAGG GTAGAGAGCA ACGCCAATAG GCGTTAGCCT TGCTTGATAG GTAAGGCGAT7920CAAGTAGGTA ATCTTTTTAA AGCACTCATC ATTTTTTAAT TCTATCTCTG CCTAAGACAA7980ATATTTTTCA AACTGAAGTT TGTTAGTTAC ACCGAGATTT TTTGTTTGTT TTCCACATCA8040AGCATTTTGC ACTCCTTTTT CTTTCATGTA TTTTTCAAGA TCAAATTTCA TTAGCAAATC8100CCTAACATAG TCGTTATATT GTTCAATGGT TTTTAGATTA GCATAGAAAT TCACTTCTTC8160ATCTGTCATC ACAAACAATT TCAATAGTGG GAGTTTAGTG CCATACAATT CTATTTGCGA8220GTCCATTTCT CTAATACTCA TCTTGAACAT GATGGGCTTA CTGAAGAAAG GCAGAAATAT8280TCCAAGAATT GATCGATCAA ATTTAGAGAA TAAGAAGTTA GGATTCTTTG CAATCGCATG8340CAACAGCTCC ATAGTCTCAC GCTCAAGCAA TTGATAAACC TTTTTAAATT TATCAACACC8400AATGCTTTTG ATAACAGCGC GATAATCTTT CATGTAATTT CTTAGAACCT TATCTAGTGC8460TCCCTTGTTT GTATGCATCT GTTTGAAAAA ATCATGCATG GTTTTTATTT GATCTATGAT8520
TTTCTTTTTT TCATCAGTGT TTAAAAGTTC GTTCTCTTGC AAACTCTCAA TAAGTTTTTC8580TTTCTTATCT TTTGAGTCAG CAATCATTGA GAAGAGTTTT CGCATGTTAT TACTCATATC8640GCTCTTGATC CCTTCAATGG ATTTTGTTTC TCTTTAGATT GTTCAAATCG TAAAGGTTTG8700TATTAAAATT ATACAATATC TGTTGTGTGA AAATTTCAGA GCGGCCATAA TTCAAAGAGC8760AAGAAACTAT TTTTTAACCA TAAAATATTG TTTCAATCAC TCTTATATCT AGTTTTTCAA8820AACCTACAAA AAACGCTTTC ACAAATAGCT CTAAAAACTG ATTTAAAAAA ATTTTAATGC8880TATAAAAAAG CAAGATAAGT CGTAAAAATG CGTTACAACT AAAATAAAGG AATAACTCAA8940TTTTCAAAAA GGAGTCTTAA GTTACCATTA TTACGATGTT AAACAAGTTC GTAATTAAAA9000TTCTCAAGAC CAAATACATT GGAAAAAAAA CCATATCAAT GGCATAGAGA GTTTTTGATC9060CTTTTAAAAA GGAGTGTGAG TTTAGGTATA AGGAAAAACT ATGTATCAAG CCTTGTTAAA9120ATGGATTAGA AAAATCCGCT TTAAGTTGTC TTGACCCTTT TTTAAACTTT CTTTATTTTT9180AAAAACCCAT AGCAATCTTA ATACCAAACT CCATGATTTT AAATTTATGT GTTGGCAATT9240TTGTTTTTGG TATGGAGGTA GTTTTCCATT GAAAAATATT ATTATAATCT CTTTATTAAA9300TGATAGGCTT GGTTATAGGC AAAAGGAGTG TTGTAATTTA TTGGATTTCA TAGAAGTATC9360TTAACACTTT TTAGTTTAAA ATGGTCTATT GTTAGGAGTT GTGGGGACGC TTCAATGTTT9420AGCAGAATAC ACTAAAAATT AAATGGATGA GATAGAACGA TACTAAAAAT AGCCTAATGG9480GCTAGGCACT TACATATGGT GCTTTAAAGG ACGGAGTTTT TCGCTAGTAA GATAAAGTTA9540TCATTAGAGC TTGAGATACT TATAAGAACC AATTTTGCCA TTGAGTCATA AGTATGATTA9600GCTTCATTGT GAAATTTGCA TGGCTTAAGA GATAGTATTT GCTTATTACG CTGAGAGAAA9660CGAGTAGCAA AAGATAAGTA GTGTAATAAA AAAAGCTAGG TTTTATTATA AGAGCAAATA9720AGAATAATAT TGGATAAACT AAAATCACCC CTGCCCCATA AGAAAAAAGC CCTATTAAAA9780AACCTATAAC GATAGAGCTG ATATTGAACA GCCTATAATA AAGGCTGTAC TTATCTAAAT9840GTTTGTTGAA AGAATGTTTG AATTGTAAGA AGTTTTGTTT TAATTTGCTA GTCGGCTGTT9900TCATTTTGGT TTTTAAAGAA ATAGTTCAAG GCGGTGAACC TATAAAGAAG CATAAAATAA9960TAAATATTTT ACAAAACCAC CCTAATCTAA CTCCAAATCT CAAAGAATAC CCCACTTGCT10020GATACTAACA TGTGGCATAG CACAAACCAA CGATTTGTTT GTTTATGCCA AACAAAGAAC10080AGAAAACATC TGTTAAAGAA TAAAGGAGTT TTCCATCTAA AAAACACAGA TCCATTTTAT10140AAAAATAATC TTAAGAGAAA CTTAAAAAAT ACCAACAAGC CGCATACAAG CAAGAAAAAC10200ATAACACTAT AAGACCTAGA TTTCACTTAC CTTTTGGATA TGGAAAAATT TTGATCCATA10260GTTTTGTAAA AATTGTGGTA AAATGCATTG ATACTTTTTG AAATTTTAAG GTTACAAAAA10320TTATAAGATG CTTGCAAAAA TCGTTTTTGG CTCATTGGTT GCGTTTGGAG TTTTGTCGGC10380TAATGTGGAG CAGTTTGGTT CATTTTTCAA CGAGATAAAA AAAGAACAAG AAGAAGTGGC10440TGCAAAAGAA GACGCTCTTA AGGCTAAAAA GAAACTCTTA AACAATACGC ATGATTTCTT10500AGAAGACTTG ATTTTTAGAA AACAAAAAAT CAAAGAGCTT ATGGATCATA GAGCTAAAGT10560TCTTTTAGAA TTAGAAAACA ATATACAAAA AGAGAAAAAG AGGCTTTAGA GAAAGAGACA10620AGAGGTAAAA TTCTCACTGC TAAGTCAAAG GCTTATGGTG ATCTAGAGCA AGCCTTAAAA10680GATAACCCTC TCTATAAGAA ACTTCTTCCT AACCCTTATG CCTATGTTTT AAACCAAGAA10740ACATTCACCA AAGAAGATAA AGAGCGTTTG AGTTATTACT ACCCTCAAGT GAAAACGAGC10800AGTATTTTTA AAAAAACTAC CGCTACCACT AAAGATAAGG CTCAGGCTTT GCTTCAAATG10860GGTGTGTTTT CTTTAGATGA AGAACAAAAC AAAAAAGCGA GCCGATTAGC TTTATCTTAT10920AAGCAAGCGA TTGAAGAATA TTCCAATAAC ATTTCTAATT TATTGAGCAG AAAAGAATTG10980GATAATATAG ATTATTACTT ACAGCTTGAA AGAAACAAGT TTGACTCTAA AGCAAAAGAT11040ATTGCTCAAA AAGCTACTAA CGCGCTTATT TTTAACTCGG AACGCTTGGC GTTTAGCATG11100GCGATTGATA AGATCAATGA GAAATACTTA AAGGGCTATG AGGCTTTTTC TAACTTGTTG11160AAAAATGTCA AAGATGATGT GGAATTGAAT ACTCTGACTA AAAACTTTAC CAATCAAAAA11220TTGAGTTTCG CTCAAAAACA AAAATTGTGT TTGTTGGTTT TAGACAGCTT CAATTTTGAT11280
ACCCAATCCA AAAAATCTAT ATTAAAAAAG ACTAATGAAT ACAATATTTT CGTAGATAGC11340GATCCTATGA TGAGTGACAA AACAACTATG CAAAAAGAAC ACTACAAGAT ATTTAATTTC11400TTCAAAACAG TGCTTTCTGC ATACCGAAAC AATGTTGCCA AGAATAATCC CTTTGAATAG11460GAAAGGAGAC ACTCTTGAAA AGTATCTTCA AAAAACTAGG TTCTGTCGCT CTTTATTTTT11520TAGTTATTTA TGGGGGCTTA AACGCTATCA ATACAGCATT ATTACCGAGT GAATACAAAA11580AATTAGTGGC TTTAGGTTTT AAAAAAATCA AAACATTCCA TCAAAGACAT GACGAAAAAG11640AAGTTACAGA AGAGGAAAAA AAATTCGCCA CTAACGCTTT GAGAGAAAAA TTACGAAATG11700ATAGGGCGAG AGCGGAGCAA ATTCAAAAGA ATATTGAAGC GTTTGAAAAA AAGAACAACT11760CTTCTATTCA AAAAAAAGCG ACTAAACACA AAGGACTACA AGAATTAAAC GAAACTAATG11820CTAACCCTTT GAATGACAAC CCTAATAGCA ATTCTTCCAC TGAAACCAAA TCCAATAAAG11880ATGATAACTT TGATGAGATG ATCAATAAGG TGAATGAAGC TTTTGTGAAA CCTGCTGCTC11940CGCTTGTGCC TAATGAGTGG AGAACACCTG AAATTGAAAT CATTATCAAT GAGTGTATTA12000TTTCAAGCAA CGATTACGAT GGGTTAAGAA AGTGTTTGAT CAAAGGCATC AAGGATCAAA12060AAATTCTTGC CCCCTTATTA GAAAAAATTC AAGAAATAGA GACAGAAAAT AACAAGTTTT12120CTAGACAACA CTTGAGTGGT TTAAAACTCG CTCTTAATAA CAGCAACAAT AGAACCTTTC12180TTATAGCTTC ATGCGCTATT TGTGAGAAGA GAAAAAAAGA AATGGAGCAA GAAAACAACC12240ACCAAGATAC TACAAATGCA AGTGAGTTTG GAGCTACTGA TGCAAAAGAA AATGAAGCAA12300AAGATGCAGC ATTCTCAAAC AATCGCTCTA AATCTGAACT GCCCAATAGC GTCATTAATC12360AAATAGAACA AAGCATCGCT CATGGAAAAA ATAGCGATCA AAATTATTAG CTCAAAAAAA12420CAACTAGAGA AACAAATCCC AAAGGTTAGA AATCATAGCT TGTTGTCTCA GAAAAATTAT12480TTAACAATGA TCTTGCTTGA TTGCCTTTCT TGTAGGTATT GCCGCTTGCT TTGTTCTAGG12540GATATTTCTA ATGCGTCCAA CTTCTCTAAA TAATTTAAAA AGACCTTGTC GTGAGCTAAC12600ATGAGCTTTC TGATTCCTTT AATGAAATTT TTATTCTTTG GGCTTTCTCA CAAGCGTTCG12660GAAGCAGGAT TAAGGAAGTT GTACCTCCAA TGTTGCTCTG ATACGCCTTT AGGAGAAGTT12720TCTAAACGCT CTCTTATATT TGTTTTTCTG GCTCGATTTT CAGCTTCCCT TCACAATAAA12780AACTAAAACT TTATCGGTTA TTCCGCATTG CTGCTCAGCA GTATTTTGGT CTAAGGGATT12840GATTTTCATA TAGGTTAATA AAAGTTCAGG GCTAGACATA TAAGTCTTGA AAATCACATC12900TTCTGAGATG AAAAATAACT CATTCGCTTC AAAATTGGCT TTCAATAACG CTAAATCTCC12960CCTCAAAGCA ATGGCCGCTT TTTTGATGTT TAGAGCATCT TCTTTACCTA TTTTATCATT13020AGCGCTAGGG CTAGTGGTTG AAAAAATCTC ATCTAAGTTT TTGAGCACTT GCTGGTTGGT13080CTCTTTGTAG GTGCTATCAA GTTGCTTTAA ACCGCTTGTT ATATCTTCTG CCATCAAAAC13140AGACAATAGC AAAAAAGAAG ATATGGTATT TTTCACGAGT GTTTTCATTT GACAATAACT13200TTAGAGCTAG CAATGTTTCT TGCTGTCGTT TCTCTCTCTA ATTTCAGTTG TTCTTCCCAA13260AGATCGGCTT TTTTTTCAAG ATTCTCTATA TAGTTTAAAT GATTTTCTGC GTTTAAGATA13320GCAACTTCTA TGAGCGCATT CAAATCTACT GATCCTTTTA AGGTTTTGAT TTCTTCATTG13380ATCCCATTCA AATAAGCGAT ATTTTGAAAA TCTGCATCAT TTAGTTTATT TTGAATAAGG13440GCTACAATCT TTCTGTAATT CTGAATAACC TGTTCCATAA GGCATGCTGA AATTTTTAGC13500CCATCAAGAT AGGGGCATTT TGTGGGCGCT AGAGTAAATG TTTCAATGAT TCCAAATGGT13560GCGCCCATGC TTGAAAAAAA ACTAAGAGCA GGCTCGTAGA TGGTGCTTTG AAACAAAGCC13620TGGCCTGTTA GGGAATTGTA GTCAATAAGG GTCGCTTTTT GCATAGCCAC TTTCAACCAT13680GTTTCAAAAC CTTCTAATGT TTCTTCAAAC GCCTTGATAC CAATCGTATT GTAAGCGATG13740TATTTAGCGT TGTCAGAAGA ACTTCCTAGA GCTTGAGAAA GCATTTCCAT TTGTGTTTTT13800AGGGTAACGC TCGGTTCAAA GCTGTTTTTT AACGCTTCTA AGAGAGCGTT TTGCTGGTTC13860ATTTTGAGCT TGATTATTTC GTTATTTTTT TGGAGTGCGA TTTGCATGTT TTGGATTTCT13920GTTTGGGTAT TAATTTTTTG TTTTTCCACG ATCATGTTGA CATTTCCTCC CAATGCACTA13980AGCGCGCTTG AATACCCTTC CATGATGCCA AGCAAGATGT CTGAACCTGC AAAAAACCCC14040
CCTGTCATGC TATTGACACC ATTAATAACG CCATTAGCCC CTTTTAACAT AGCGCTTATA14100GTTGCAAGCT GAGTTCTCAA TTGTCCCTCT ATTTGCGCTT GAATGGCTTT TTCTTTGGCA14160CTAGATTGAG CCTCTATGGC TTTTAATTCG GCTTGAGCGG TTTTTTGTTT GGCTTGTGCA14220TCCGCCTGAA TGGCTTTTAA GGCAGATTCA AGCGTTATTA CTGCCCCTGC ACCATTCAGA14280AACAAACCAC AAAAAGTTAA GAAAGAAAAT ATGCTTAAAA AATATTTCAC ATCTCTTTCC14340TCACTTCACG ATTATTTTAG TTTGCACCCT TTCTGTTAAG TAGCTATCTT TTTGCCCCTT14400AAGTTTGTCT TTGATGTAAT CAAGATAAGT CAAATGCGAT TTCAAAAAAG ATTTATTCGC14460TACTATATTG TAATTATATA GCGAGCTTAT GTTAGAAATC GCTTGAGTAT CATAGGTGCT14520AGTAGCTAAT CCTGATTGAT TAAGTATCAT TTGAGAAGCG TTTTGCAACA AATTGGTATT14580GTTTTTCACA AATTCTATAT AGTATTCTCT CAAAATTTCT GCTACTTTTT CAGCATAGCA14640ATAAACGACA AGAACTTTGT CCCCAGTAGG GCATGCAGGA GTGGTCATAG GATTAACGCC14700TGAAGTTAGG GCATTAGCGG CTAACGCTTG GTATTTGGCA TAAACAGTGG GCATAGAGAC14760ACTCATAGGA GTCATAGAAA TTTGCATGCA ACTAAAAAAC ACTTTTGATG AGCTAGCAAG14820CGCACCTAAA GCGGTACAGC TATCAAGGGA ATCGGCTGTA TCATTCATTG AGCTGTTGCT14880TGCTTGAGAA CGCAGTTGCT CTTGTAGGGC TAGGGTGTAT TTTGGTGCTG CACTTGTAAT14940ATTGCCTAAG ATACCGCTCA TCATTTCAAC CGTTGTTGGC ACACTAGGAA CAGCGATTTG15000ATTCGTCGCA TAAGCTTCAA TAGCGCTAGG ATTTTTAGGG GTAGTGTTAC TCGCTAAAAT15060GCTTGCAATC TGACTATTAA CAGCACCAAT TTGCGCGCCT TGCGTGTTGC CTTGCGCGTT15120AAATTCCCCT GTTAATTTGC TGATATTTAA GATATTGTTC CCCACAGCCA TGCTTTGATC15180GTTAAAACCT TGATACAATT GGTTGTATTG TTGGTTAGCG GCTTTCATAG GCATGCTTAC15240GGCTTCAGCG ATGCTTTGAT TGTATTGGGT CATGATAGCG GTCATTTGTG GATTCGTAAA15300TCCCACAACA ATAGGAATAA TCGCTGCTGA CATAGCACCC GCTACTATTC CTGCAAATGG15360CCCCGCAACA CCACTTGTGC TTGAGATGAT TGAGGAAATT TCCGATAAGA AGCCTGCAGA15420AGATGATTCA TATATAGCTT GCGTGCCTGC CATGTTAAAA CCCCCCTAAG CTTGGTGGTA15480AAAACGATGA ATCTGGATAT GTTGGTGCAT AACCATACAT GAAAGGATTG TTTGGGCCGT15540AATCGCCCAT CATTTGGCTC ATGAGAAGAT TTTGAATGCC CCACATCGCA TTGATACCTA15600GATTATCATT AGGTTGAAAA CTCCCTAAAC TTATGTTATC AAACTTGATA TTAACATTCT15660TATCATTATA GTCATTGAGT ATGGCCACTT TTTGCTCTAG GGTTTCTTTA GGGATCTCTA15720TTTTTAGCTG ATCTCTAGAA ACAAGCCCCA CGCTATTTAG TGCCATATCT TCAGGACTAA15780TATCTTTTAT ATCAGTGTTT TGGTCAGCGT TAAGCGGAGC GTTAAACATG CCAATGATAA15840GACACCAAGC AAATAATAAT TTAATTTTAT AAAAATTCGT TTTCATACTT TTGACTCCTT15900TATTCTTATT TTTAGCACTA TTCTAGCGCA TTAACACCAC TCAATCGCTA TTTTTGTTTT15960GATTTTTTTG ATCGAGCATT TTGTTTGTTA CTTCATCAAT GTTTTGAAAA TATTTTTCAA16020AAAGCTCTTT CTTTTTAGCT TCAACGCTCA TATCAACCCT GAATCCAATT AGGAACAATG16080GAGTCCATGA TTAAATGCGT GAAGTCATAG GCATGATTTG TTGGGTATAT TTTGTTTTGA16140ACATAGTATT CTAAAAAATT CGCTTGAACA AAAAAATTCT CTATATCGCT CTGCATATCC16200TCGCTTATGT TGTTATTGAT AGGTTTTTCT AGTAATCTGA GAATCCTATA CGACTGCATG16260ATAGTTTCCA GCATGAAGTA AACATAAACA TAAACTAATG AGACAAAAGA ATTATTTGCT16320TTAAACGAGC TTGCATCATT TTTCCCACTT TGAAGAGGAA TTAATCTTGA TAATGTTTTT16380TGGGGATCTT GCCCATCGTT TATTTCTTTA AAAAAGCGGA AGTCTAAATC CTGATTATTG16440AGAAATGACT TGACAAAGTG AAGATTAGCA TTGAGACTAT CTATGAGACC TGAATAAAGG16500TGTTCTGTTT TGACATCATC TATATTTAAA ACATTCTCAT AAAACACATT GACATGGTCT16560TCTAAGAAAT TAGAAAAATC ATAAAGAGTG GTAAGGTTTT GTTCAGTGAT TTCGCCTTCC16620ATTTCTTCTT CTATGAAGTC CAATTCTTCT CTCAGTTCAA AAAGATAATT AGAAAAACTA16680TCCAAAATCG TCAAGACATC ATTTTCTAAA ATTTTCAATA ATTTTTGTTC ACGCAAACTT16740TGTTTCATTT TAATACTCCT TTATTTGTTG ATACATTTGC CTCAAGGCCT GATATTTATC16800
TATGATACTA TGGTTTTGGA TAATCTTGTC AATCTCTTTG ACAAATACGG TATCTGTGGA16860TAAAATTTTC AAATATTCTT TAGGAATGCC TCTCAAATTA AAACTAGCGA TAACGCTAGG16920GCTTCCATCT TGTTTGTAGA GAATTTTCCT ATCTAGCCCC TTAGTGAGAT TTCAAATTCT16980TTTCTGTAAC ATTAGCCAAT CTTTGGTAAT CCAGAAAGAT TTCCCCCCAT CGTTTCTTAA17040AAAAATCTTT GTAGGGCATT GTTCTCTAAT AGTATCAGCA ATAGGGCAAG CCAAAAGATC17100AGTGATGCTT TGAGTCGCAA GTCTAACAAT AGCGTTTCTT TTCCTTGCAG TTTTTAGCAT17160GTCTCTTACA AAATAAGCGA CCTTTGGATC GCCTAAATAT TTCCAAGCTT CATCAATATC17220TAAAACAAAT CTACGCCCAT CCATTGCCTC TTGAATACGA GCGAAAAGGT AAAAACAAAT17280AAAGGGCGAA ACATCATTAT TGTCTAGGAA ACTTGACCCA TCAACGCCAA TAATCGTTTT17340TGAAAAATCT AAGCGATCTG TTGCTTTATT ATCAAAAAGC CATTGAAATT CACCATTGGT17400TGATTTGCAA AAAGGCGCTA ATCGCGCGAC AAGCCCATTA GGATCATTGT GGTCTTTTCC17460GAAAGCATTA ATAAGTTGAG TGATGGGATA ATCTAGGTTC ATATTTCCTG TGATAAGGTT17520GGTTACTGCC GCTGCAAGCG TATTAGAATC AGCTAGGCTA AAAGAGATGC TGTTGCCATT17580TTCATCTTTT TCATCGCTTT TGGTGACTAG GTTTTTCACA AGCTCTTTGA CAACAGAAAT17640AGCTGTTTGT TTTTGCTCCA TTGTTGCATT GGATTTTTGC ACACAAGCCG CCCAAGCAAA17700AGGATTTAAT CCTGTATCTG TCCCTAGCTC AATCTTGACA TACTCCCCAC CCATTGCGAC17760AATATTCCCA TAAGCGCCAT AATCTTTATC CATATAGACC ATAGTGAGCT TTTGCTTGTC17820TTTACTGACA TTAGCAGGAA AATTGTGAGC AAATTGTCCC ATAGCGTTTA GAGTCATTGA17880CATAAACACT GTCTTACCTG AACCGGTTGA GCCAAGTATC AAAGTGTGTC CTGCTGAAGC17940TGAACCAAAA TCAGTAGGCA TGTGGAAGTT CAGATAAAAA GGCGAATTGA TCTCGCTTTT18000TAGCGTCATG ACGCTATTAC CCCAAGCGTT ATTCTCTTGA TTGCCATCAA AACTCATCGC18060TCTCATAGCG ATGAAATCAG CAAAATTATT AGAAGTTACA TCAAAAATAA AAGGAAGCGT18120GATAAAAGAG CAATGTTTGG CAAAAAAGTA ACTTTCCATA GAGAAAGTGA GTGCGTAGGC18180TAAAAAACCT TTCAGCGTTA AGAACTAGAG ACGCATTCCT TAACGCTTTG TTTCATTTTT18240TCAAAGCTAT CAGCAAACAG CACTAAAGAA TTACCATAAC TGCCTAGCGT AATATCACCA18300TTACCCACTA ATTCGCTCAA GCAACCTAAA GTCATGCCCT GCTCTTTAGA ACCCCCACTA18360ATAATAATCC TTCTAGAGGT GAAAGCTAGT TTGTCCTTTA AAACCTGTGA GTTTTTAGGC18420GAATAAGCAT GCATAAAGAT AAATTCGCTG TCTAGAGCGT TGATCTTGTC AAACAAATCG18480CTCTGTGATT TAGGGGCGTA TTCACTAATC TCAATAGCGC TAAAATATTT TTCACTCAAA18540TCGTCATTTA AGATTTTTCC ATGTTTATTG GCAAAATAAA CTTCTTTCAC CCCACCATGC18600ATTTTTTCCT TGAGATACAA GTCTTTTCTG TTGCAAATAA AAGGGGCTTC ATTCATTCCC18660ACAAGAAAAT TGTAAAACTC GCATTGTTTG GAATAAACAA CGCCATCTTT AATGTATTCT18720TTTAATCTAG TGGGGTGGTA TTTACTCAAT AGCTCTTCTA TGAGCTCTAT CCTATCCTTG18780AAGTTTTCAA GCTTGGCTCT AATAATCCTT TGAAACTCTT CAAAATTATT GTCTGCAAAA18840TGCTTTTTAT TCATAACGGG TTCATTGAGA GTGTCTAATA AATCTTGCTC TATGGTCAGA18900AAAAAACTAA TATCATAAAA ACTTTCTCTT TTTTGCTTCT CATTATAGGC TCGCATAAAA18960TCATTAGAAA AAATAAGACT ATAGTCCCTA TTGGTTTCAT CAATAACGAT TTTCTTTTTA19020ACGGTGTGGA AATAAAATTT GAATTCAGGG GTAACAAAAT TCCTAAAAAC GCTATAAATA19080GAAGCGTGTA ACTCTATGAG ATCTTTTTTG GAAGTGGTTA AAAAATCAAT GCCCCCCAAT19140TTGATTGTGC CTAAAAGAGA ATAGTTGTTA GTGAGGATCA CCCCATCATC TAAAAAACAT19200TCATAGTTAT TCGCTAGATA GGAGTTTGCA GCGCTCACAA GTCTGTCTTC TCTGTTTGGA19260TTTAAGTGGA TGTCATTAGC CATTTCTTTA CTAGGCTTCA TGGAAAAAAT GCTCATGAAC19320GCTTTGTTTT TCACGCCCTT AAACAAAAAA GGTTTTTTAA ATTTCATCGC TCGCTCCATT19380CTTTGATAAA GCCTATAGTC TTTCTTGAAT CCAAGAGCTA CAAGCACAAT AACAATCGCT19440ACAATCAAAA CAGGTTCATA GGCTTGAAAA AGAATAACAG ATAATACAAT GGTTACAAAC19500AATATAAATA TAGAAGAATA AATAAAAGTT TCAGGGAAAC CAAACAACCT ATTCCCCCCA19560
TCAAACAAGA CTTTAAAAAA GGGATTGACA CCTTTTTGCA TGTCTGCTTT AAGCTCTTCT19620ATTTTTTGAA ACTGCCGCTT TTGAACCTCT TGCTCTATGA TTAGCTTTTT TTGTTCGTCA19680GCTTGCTTGC TTGCCACAAA CACCTCTCTC TTTATAGATA TACCGCTTCA CATGTAATCG19740TATAAAAGAT TTTTTTGAGA GACTCTATAG CGCTAATATG TTTCAAAAGA TCATTAGGGT19800CATAAGAATT GAATACGGCC AATAAAACAT TGTATAGCTT ATCATCGCAT AGAATTTCTC19860TTGTTTCTCC GTGCAATGAT AGGAAGCAGC GTTGTTTGTT GGTCGTGCTG ATGCTTTTGA19920AAGTAAAAAA GTCTTTCACT TCAGGATTGA TCTGCAATTC CACATTCAAT CCCATTTCTT19980TACCCTTTTC ATCAAAGATT TTTTCAATAA TTGGATCGTA ATGCTTCAAA TCCTTTATTT20040TTTTAAGGAC TCTATTGACA ATCACGAAGT CAAAAACTTC ATCTTTGATA ATGTCGGGAT20100TGACTTCTTT GAAAGTTACT TTCTTGTCTT TCAAATTTTT GATAGTCGCT TTAAAACTAT20160CAAAATCTAA ATTCGTATAA ACACGCCCAC TAGGAGTGTT TTTTTCTTTT TCTAGTGCTT20220CTTTTTTGGC TTGATTATCA TCATTTGCCA ACCCATACGA ACTGAAAACA ACGAGACTTA20280AGAGAACTTT CAAAAAAAAG TCTCTTAGTT TTTTATTACT ATTATTGTTG ATCAATTTAG20340CTAGCTCCTC CACCTTCGCC AATATTGAAG CCAAACTTAG TGCTCAAATA GATAATACCG20400CCTGCCACCG CTAACATAGC TATGGGTTGC GCGTAAGCAA AAACAGTCGC CTGACCTCTT20460TTGATGTCAT CAGAGATTTT CCAAATATCT GCTATGCCTT TGACCCCTAG AGCGCAACCA20520CCTACGATCG CTAGAACAGA AATGATCTGA ACAACCAAAG TTCTAGTCTC AGTAACGCCT20580GTTGCAGGAC TGTTGAACGC CATTAAAGGA TTGGTTGTTA TCACTAGCCC TAAAGTTACT20640ACAACTTTCT TGTAGCTTGT CAGTGATTCT TGTAAGAAAT TTCATGCGTT TCCTTTCAAA20700TTGAAATCAA TCGCTTAAGT ATATCAAAAA AAAGTATTTT TATGCTATTC ATACAAGCAC20760TACTTTATTT AAATCAAAAC CGACACTTTT GTTTGACAAT TGGCACAATT TAGAACAATA20820AACCTACTTG TCCCAACCTT TTTTCTTTCT CAAGTCATTG TAGAATTGCA GATCTTTAGG20880ATCTTTGATA TATTTTTTAA TCGTCTCAGG TTGAAACCTA AAAACAAGCA AAAACAAACC20940CAAGCTGATC AGAGTGAGAA TAAAGCTCCA TTTTAAGCAA CTCCATAGAC CACTAAAGAA21000ACTTTTTTTG AAGCTGTCTT TGAAAATCTG TCCTATTGAT TTGTTTTCCA TGTGAATCAC21060AAACACTTCT TGTTAAAATT TGTCTATTTT AGCAAGTTTT TGTCAATTGC GGATAAAAAT21120GTGAATCGTT CCTAGCTTTT AGATGCTTGC AACGACTGGA ATTTTTCAAT ATTAACAAGA21180TTAATGAAAA AATGCCTAAT ATTGTAGTAT AATGAGAGCG TTCAAAGACA TGAATTGACT21240ATTCAAAGCG TGTATAGTTT TTAGCAGTCT TTGATACCAA ATTTAGGTAT AGTAAGGAGA21300AACAATGACT AACGAAACCA TTGATCAAAC AATAACACCA GATCAAACAC CAAATCAAAC21360GGATTTTGTT CCGCAACGAT TTATCAATAA TCTTCAAGTA GCTTTCCTTA AAGTTGATAA21420CGCTGTCGCT TCATTTGATC CCGATCAAAA ACCAATCGTT GATAAGAATG ATAGGGATAA21480CAGGCAAGCT TTTGAGAAAA TCTCGCAACT AAGGGAAGAA TACGCCAATA AAGCGATCAA21540AAATCCCACC AAAAAGAATC AGTATTTTTC AGACTTTATC AATAAGAGCA ATGATTTAAT21600CAACAAAGAC AATCTCATTG CTGTAGATTC TTCCGTAGAG AGCTTTAAGA AATTTGGGGA21660TCAGCGTTAC CAAATTTTTA TGAGTTGGGT GTCCCTTCAA AAAGATCCGT CTAAAATCAA21720CACCCCAACA AATCCGAGAT TTTATGGAAA ATATCATACA ACCCCCTATC TCTGATGATA21780AGGAAAAAGC GGAGTTTTTG AGGTCTGCCA AACAATCTTT TGCAGGAATT ATCATAGGGA21840ACCAAATCCG ATCGGATGAA AAATTCATGG GCGTGTTTGA TGAATCTTTG AAAGAAAGGC21900AAGAAGCAGA AAAAAATGGA GGGTCTACTG GAGAGCCTAC TGGTGGGGAT TGGCTTGATA21960TTTTTTTATC ATTTGTGTTT AACAAAAAAC AATCTTCCGA TCTCAAAGAA ACGCTCCATC22020AAGAGCCAAG GCCTGATTTT GAACAAAATA TAGCCACTAC CCCCCACCGA CATACAAGGC22080TTACCGCCTG AAGCTAGAGA TTTGCTTGAT GAAAGGGGTA ATTTTTTTAA ATTCACTCTT22140GGTGATGTGG AGATGTTGGA TGTTGAGGGA GTCGCTGCAT AAGGCATCCC AATTACAAGT22200TCAATCAATT ATTGATCCAC AATAACGCTG CTATCTTCTG TGCTAATGGG GGGTCATAGT22260AACATAGAAC CTGAAAAAGT TTCATTATTG TATGGGGATA ATGGTGGTCC TGAAGCTAGG22320
CATGATTGGA ACGCCACCGT TGGTTATAAA GACCAACAAG GCAACAATGT GGCCACACTC22380ATTAATGCGC ATCTTAATAA CGGCAGTGGG TTAATCATAG CGGGTAATGA GGATGGGATT22440AAAAATCCTA GCTTCTATCT CTATAAAGAA GATCAACTCA CAGGTTTGAA ACAAGCGTTG22500AGTCAAGAAG AGATCCAAAA CAAAGTGGAT TTCATGGAAT TTCTTGCAAA AAACAATGCT22560AAACTAGATA ACTTGAGCGA GAAAGAGAAA GAAAAATTCC AAACTGAGAT TGAATATTTC22620CAAAAAGACC GTAAGGCTTA TTTGGACGCC CTAGGGAATG ATCACATTGC TTTTGTTTCT22680AAAAAAGACA ACAAGCATTT AGCTTTGGTT ACTGAGTTTG GTAATGGAGA ATTGAGCTAT22740ACTCTCAAAG ATTATGGGAA AAAACAAGAT AAAGCTTTAG ATGGGGAGAC AAAAACCACT22800CTTCAAGGTA GCCTAAAATA TGATGGCGTG ATGTTTGTTG ATTATTCTAA TTTCAAATAC22860ACCAACGCCT CCAAGAGTCC TGATAAGGGT GTGGGTGCTA CGAATGGCGT TTCCCATTTG22920GAAGCAAATT TTAGCAAGGT AGCTGTCTTT AATTTGCCTA ATTTAAATAA TCTCGCTATC22980ACTAATTATA TAAGGCGAGA TTTAGAAGAT AAATTGTGGG CTAAAGGATT GTCCCCACAA23040GAAGCTAATA AGCTCATCAA AGACTTTTTG AACAGCAACA AGGAATTGGT TGGAAAAGTT23100TCAAACTTCA ATAAAGCTGT AGCTGAAGCT AAAAACATAG GCAATTATGA TGAAGTGAAA23160AAAGCTCAGA AAGATCTTGA AAAATCTCTA AGGAAACGTA GAGCATTTAG AGAAAGAAGT23220AGCGAAAAAA TTGGAGAGCA GAAACGACAA CAAAAATAGA ATGGAAGCAA AAGCTCAAGC23280TAACAGCCAA AAAGATAAGA TTTTTGCGTT GATCAATAAA GAGGCTAGTA AGGAAGCAAG23340AGCGGCCGCT TTCGATCCGA ATCTTAAAGG CGTCAGGAGC GAATTGTTTG ATAAACTTGA23400AAATATCAAC AAGAATTTGA AAGACTTTGG CAAATCTTTT GATGAACTCA AAAATGGCAA23460AAATAATGAT TTCAGCAAGG TAGAAGAAGC GCTAAAAGCC CTTAAAGACT CGGTGAAAGA23520TTTAGGCATC AATCCAGAAT GGATTTCAAA AATTGAAAAC CTTAATGCAG CTTTGAATGA23580TTTCAAAAAT GGTAAAAATA AGGATTTCAG CAAGGTAACA CAAGCAAAAA GCGACCTTGA23640AAATTCCATT AAGGATGTGA TCATCAATCA AAAGATAACG GATAAAGTTG ACAATCTCAA23700TCAGGCTGTA TCAGAGACTA AATTAACAGG CGATTTCAGT AAGGTAGAGC AAGCCCTAGC23760CGAACTCAAA AACTTGTCAT TGGATCTTGG AAAAAATTCT GATCTACAAT CCGTTAAAAA23820TGGTGTAAAT GGAGCCCTAG TCGGTAATGG GTTGTCTAAA ACAGAAGCCA CAACGCTCAC23880CAAAAATTTT TCGGATATCA GGAAAGAATT GAACGAGAAA TTATTTGGAA ATTCCAATAA23940CAATAATAAT GGACTCAAAA ACAACGAAGA GCCCATTTAC GCTAAAGTCA ATAAAAAGAA24000AGCAGGACAA ACAGCTAGCC CTGAAGAGCC CATTTACGCT CAAGTTGCTA AAAAGGTGAG24060TGCAAAAATT GACCAACTCA ACGAAGCTAC ATCAGCAATA AATAGAAAAA TTGACCGGAT24120TAACAAAATT GCATCAGCAG GTAAAGGAGT GGGCGGTTTC AGTGGAGCAG GGCAATCAGC24180TAGCCCTGAA CCCATTTATG CTACAATTGA TTTTGATGAG GCAAATCAAG CAGGCTTCCC24240TTTGAGAAGA AGTGCTGCAG TTAATGATCT CAGTAAAGTA GGGCTTTCAA GGGAACAAGA24300ATTGACTCGT AGAATTGGCG ATCTCAATCA GGCGGTATCA GAAGCTAAAA CAGGTCATTT24360TGACAAACTA GAACAAAAGA TAGATGAACT CAAAGATTCT ACGAAAAAGA ATGCTTTGAA24420GCTGTGGGTT GAAAGCGCGA AACAAGTGCC TACTGGTTTG TCAGCGAAAT TGGACAATTA24480CGCTACTAAC AGCCACACAC GCATTAATAG CAATGTCCAA AGTGGAACAA TCAATGAAAA24540AGAGACCGGT ATACTAACGC AAAAAAACCC TGAGTGGCTC AAGCTTGTGA ATGATAAGAT24600AGTTGCACAT AATGTGGGAA GCGCTCATTT GTCAGAGTAT GATAAAATTG GATTCAACCA24660AAAGAATATG AAAGATTATT CTGATTCGTT CAAGTTTTCC ACCAAGTTGA ACAATGCCGT24720AAAAGACATT AAGTCTAGCT TTGTGCAATT TTTAACCAAT ACATTTTCTA CAGGATCTTA24780CAGCTTGATG AAAGCAAATG TGGAACATGG AGTCAAAAAT ACTACAAAAA GTGGTTTCCA24840AAAATCTTAA AGGATCGAGA AATACTAAAA ACACCCCTTG TTAAAACAAG GGGCTTCCTA24900ACTAAAATAT CCTAATAAAC ACTAACACGA AAGGCTTTGT TCTTTATGGT CTGTAAGGAC24960ATTTCCTATC CTAAAAAGAC TTAACCCTTT ATTCAAGATA TTGTTCGCAG CGTTAATATC25020TCTGTGTTCT TTATACCCAC AATCAGGACA CAAATATTGC CTGTGATTGA ATTTGAGCTT25080
GGGGTTGATA CTCCCACAAT GATAGCAAGT TTTGCTAGTG TATTGTGGGG GAACTTTCAC25140TAACAATTTG CTATTATGCT GTTGTTTGCA GTCTAGCAAA CTTGTAGATG GGTAATTCTT25200TTCCAAATAT AAAAACGCTT TTCTGGATCT CTAAAAGC25238<210>119<211>11647<212>DNA<213>H.pylori<400>119CTCATATCTT GGCTGTGAGT AAGATTGACA CCGCCACGCA TAAGCAGGTT TTGCAAAAAT60TACAAGAGTA TCAACAATAC GCTTCGCAAT TTTTAGCTCT AGTGCCTTTG AGCGCGAAAA120AATCTCAAAA TTTAAACGCG CTTTTAGAAT GCATCAGCAA GCATTTAAGC CCTAGTGCAT180GGCTTTTTGA AAAGGATTTG ATGAGCGATG AAAAAATGCG CGATATTTAT AAGGAAATCA240TTAGGGAGAG TTTGTTTGAT TTTTTGAGCG ATGAAATCCC TTATGAAAGC GATGTGATGA300TTGATAAATT TATAGAAGAA GAACGCATAG ACAAGGTGTA TGCGCGTATT ATCGTAGAAA360AAGAAAGCCA AAAAAAAATC GTGATAGGCA AAAACGGGGT GAATATCAAA CGCATCGGGA420CTAACGCGCG ATTGAAAATG CAAGAAGTGG GCGAAAAAAA GGTTTTTTTA AACCTGCAAG480TGATCGCTCA AAAATTATGG AGCAAGGAAG AAAAGAGCTT GCAAAAATTG GGTTATATCC540ATAAAAGGGG TATGGATTGA AAAAAATATT ACCGGCTTTA TTAATGGGGT TTGTGGGCTT600GAATGCTGAT GAGCGTTTGT TGGAAATTGT GCGCCTTTAT CAAAAGCAAG GCTTGGAAGT660GGTGGGTCAA AAGCTGGATT CTTATTTAGC GGATAAATCT TTTTGGGCAG AAGAGCTTCA720AAACGAGGAC ACGGATTTTG GCTATTATCA AAACAAGCAG TTTTTATTTG TGGCGGATAA780ATCCAAGCCT AGTTTAGAGT TTTATGAAAT AGACAACAAC ATGCTTAAAA AAATCAACAG840CTCTAAAGCC CTTGTAGGCT CTAAAAAGGG CGATAAAACT TTAGAGGGCG ATTTGGCCAC900GCCTATTGGA GTGTATCGTA TCACGCAGAA ATTGGAGCAC TTGGATCAAT ATTATGGCGT960CTTGGCTTTT GTAACGAATT ACCCTAATTT GTATGACACC TTAGAAAAAC GCACCGGGCA1020TGGCATTTGG GTGCATGGAA TGCCTTTAAA TGGCGATCGG AATGAATTGA ACACCAAGGG1080CTGTATTGCG ATTGAAAACC CGCTTTTAAG CTCTTATGAT AAAATATTAA AAGGCGAAAA1140AGCGTTCCTC ATCACCTATG AAGACAAGTT TTTCCCCAGC ACCAAAGAAG AATTGAGCAT1200GATTTTAAGC TCCCTTTTTC AATGGAAAGA AGCCTGGGCT AGGGGCGATT TTGAACGCTA1260CATGCGTTTT TATAGCCCCA ACTTCACCCG CTATGACGGC ATGAAATTCA ACGCTTTTAA1320AGAGTATAAA AAAAGGGTGT TTGCGAAAAA CGAAAAAAAG AATATCGCTT TTTCTTCTAT1380TAGCGTGATC CCTTACCCCA ACTCCCAAAA CAAACGCTTG TTTTATGTGG CGTTTGACCA1440AGATTACAAA GCCTACCAGC AAAACAAGCT CTCTTATAGC TCTCATTCTC AAAAAGAACT1500CTATGTAGAG ATTGAAAACA ACCAAGTGTC TATTATAATG GAAAAATAGG GCTTTGCTTT1560AATTGGGGCA GTCTAAACGG ATTTTTTAAC CCATTCAGCC AAGCTTGATA CAAGTTTGAT1620TAAAAAAACT ACAGAGCTTC CATGGCTTTT TCATAGCCAA ATTCTGCGGA TTTTTGCAAG1680AATTTTTTAG CCCTACTTTT TTGTTTTCTG GTGCCAAGCC CTTCTTTATA AGCGATCCCA1740ATTCTATAAA GGATTTCCCC CATTTGTTGT TTGAGTAAAG TAATATTGCT TGAAATTTCA1800GCAATGTCTT CCCAAAGAAG TGTCTTATCT TTGATTTGAG TGGATTTATT AACAAGTATT1860CCAAATTTCT TTCCAAATTT TATAGGATTC GCGCTCAAAC TTGAAAGATC AATGAATCGC1920GATCCAATAA GCATACTAGT AAATTCCGCA AGGTTTGATC CAAACTTGTT AGCAAAATTT1980
GTGCTTTTAT TAGAAAATAT GCGAACCACA TGCCTGTAAG TCTTAAGCGC TTGTTTCAAA2040TCCTTTTTCA TCCCTAAGCC TTGCGCTTGC ATGCGTCCTA AAATAAGAGC GCCCCCTAAC2100AACCCGCTCC CATACCCTTT GATAGCGTTT TGTGCATAGA GTTGCGCTTT TTTGTAATCC2160ACTTTCACGC CCTTTCCCTC CAAATACATT TTGGCTAAAT AAACGCTGCC AAGCCCAATG2220CTGTATTCTT GCGCCAATTT AAAATCTTTA AAGGCTTGTT TGTATTGGCC TTGATTAAAA2280TGGTCTAAAC CATTTTCAAA ATAATATGTC TTATGATTTC GTGCAATTTC ATCCCACTGA2340TTTCGCGCCC TTTCATCCCA ATCATCACTT ATGCTAATAT ATTCATCAGC TATACAAAGC2400GAAAACGGCA ACAATAAAAC TAGTAAAAGC AATAAAGGCT TTAAAAAAGA GAGGGTGGTG2460CGATAAAAAT CTTTAAACAT GTCAAGTCTT TTTACAATTT GAGCCATTCT TTAGCTTGTT2520TTATCATCTT TTTTAAATTA CAAACAACAG CATAATGACA ACATGGAGAT GGTTTTATAT2580TTTTTGCTAT AATGGCATGA ATTTGTTTCA TAGTAACAAA TTAAAAATAT ACCATTATGG2640AGGTAATGCT ATGGCTGACA CAATCAATGC AACTGAAGCA ACTCATGAAA CAAAAAAACC2700AAACGCTTTT GTAAATTTTT TCAAAAACAA TTTGACTGAT AAGCGTTATG ATGCATTAGG2760TCTCATTGGA GCAGGGGTTT TATGTTGTGT CTTGAGCGGT GCTATGGGGA TTGTTGGGAT2820AATTTTTGTC GCAATAGGAA TCTTTTTGTC TTTTTCTAAT ATCAACTTAG TGAAATTAAT2880TGAAAAATTG TTCAAAAAGC AATCTAAAGC GGCAACAACT GTCAATAACG AAACCCAAAA2940ATCTCAAGCA ACAAGCGTTA CCAACGAACC AACTGAAGCC AAAGAGACTA AAGATTGAGA3000CAAAACAACG ATTTTGACTG AAGAAAGAAT GAGAGAAAAT TTCAAAAATT TAGGCGTTGT3060AGTGGTTTAT CACTCACTGC ATGACCCACA CAAGCGACCT ATAAATTCTT TTTCTCTAAA3120GACTTCTAAT ATCATAGGAT TCTCTATTTT TATCTGCAAA CACGCACAAA AAGAAATCAT3180GCAAAAAGTG GCTTCAAGAC ACACTGACGA TTCAAATAAG TTCAACATCA AACCCAAAGA3240AAAGATTGAT GAGAATGTCA TTAACGCATT AGAGACAAGT TTGAAATCTA AAGGGGTCGT3300TTCAGAAACG CTAGAAAGTA TCAAAGAAGC TAAAAACAAC AAAGAGTATG AGAATTTGTC3360CAATAGGGGG TTTTTAAGGG CTAAATTGGA TCAAGGGGTA TGGATCAACT TTCAGGAGTC3420TCTAGGCTAC AACTTGAGCA CCCTACAAAA AAAGGCAATC TCTCTGTTAA AAATCAGCCC3480CAATCAGATA TTAGAATTAG TCCAAAAACT ATACATGAAA GGGCATATAA GCTATCCTAC3540TGAATATTTG TTTGAAGAGA CTTTCAAAAA AAACTTATAT AAGATTTACA AAGACTCAGA3600AAATGATTGC TTCAACAAGA ATGTGGCTGA TGAAGAAAAA GTTCTTTTAG AGTTTCCTAG3660CAGAAAATTC ACTCCCAATG AGCTTTTTAC AGCTGCATTA CTTACCTTAA ATGCAATGGA3720ATTTTGTCTC TATATCAATT CTGAAAAAAA AGGAAACTAA TGTTTAGAAA ACTAGCAACC3780GCTGTATCGC TCATGGGCTT ACTAACCTCT AACACTCTTT ATACTAAAGA AATAAGTGAA3840GCCGATAAGG TCATTAAGGC CACTAAAGAA ACTAAAGAGA CCAAGAAAGA AGCTAAACGA3900CTCAAAAAAG AAGCCAGACA GCGCCAACAG ATCCCTGATC ATAAGAAACC TCAATATGTT3960TCTGTTGATG ACACAAAAAC TCAAGCGCTT TTTGATGTAT ACGACACCTT GTATGTGAAT4020GACAAAAGCT TGGGGGATTG GTTCGGCAAT AACGCTCTGA AAGACAAAAC CTATCTCTAC4080TCTGAGGATC TATTGAATTA CAACAACTAT CTATACCTAG AATACCCCAT TATCAAAACA4140CGAGCAGTGG GAACCTATGC GGATCTGATC ATCATCACAG GCTCACTAGA ACAAGTCAAT4200GGGCATTACA ACATGCTAAA AGCGCTCAAC AAACGAAACG CTGAGATTGT CTTAAAAATC4260AATGAAAACC TGCCTTACGC CCAAGCGACT CTCTTGATAG TGCCAAATAG AAGCGATCCC4320AATGCCCATA CGCTAGATAA GGGAGCGTCA ATTGATGAGA ATAATCTTTT TGAACAACAA4380AAACGCGCGT ATTTCAACTA CGCCAACGAT GTGATCTGCA GACCCAATGA TCAAGTGTGT4440TCGCCTGTGA GTAGATGCAG AGTGGTAGCT ATGCCCTCTA GCGATAGCAG TGCTCAGAAA4500CCTAAGAGCA TTGCTCATGA TAGCTTGTAT AGACTACAAG AGTCAGATAG CGCCAATGAG4560GCTCAGCCAT CGCTNAAGTG CCACTCAGCT CCGCTCCTGA AAACAGCGAA GAGAAGCTCA4620TAGAAAGAGC TAGTCGCTCA CTCCCTCCTC AGAGCCAATG AAGAAGAGAG GGAAAAGAAA4680CTCTTAGCAG AAAAAGAAAA ACAAGAGGCT GAATTGGCTA AATACAAGCT CAAAGACCTA4740
GAAAATCAAA AGAAACTAAA AGCTTTAGAA GCAGAGTTGA AAAAGAAAAA TGCTAAGAAA4800CCTAGAGTAG TGGAAGTGCC TATTTCTCCT AGAACAAGTA ATCCTGATGA AACAATGAGA4860GTTGTCAAGG AAAAAGAAAA TTATAACGGA TTGTTAGTGG ATAAAGAAAC CACGATCAAA4920CGAAGCTATC AAGGGACTTT GATTAGTGAA AATTCTTACA GCAAAAAAAC ACCTCTCAAC4980CCTAATGACT TGAGAAGCTT AGAAGAAGAA ATTAAGAGCT ACTATATTAA GTCTAATGGC5040TTGTGTTATG CTAATGGCAT CAGTCTCTAT GTCAAAATCA AAAACGACCC CTATAAAGAG5100GGAATGCTGT GTGGCTATGA GAGTATTCAA AATCTGCTCT CACCTCTAAA GGACAAGCTC5160AAGTACGACA AGCAAAAATT ACAAAAAGCG TTACTGAAAG ATTCAAAGTA AGTTAAAGTT5220TTGTTTGAAA AATGGATTGG TCTCTTTTTA CTTCTTAATT CCTTAGCCTA TCCATGCCAA5280AAAGTAACCA TTAGTTTCAA GCAGTATGAA AATCTTATCC ATATTCACCA AAAAGGTTGC5340GATAATGAAG TGGGTGTGCA GAACGCTCAT CTCTATCGCT TTATTGGAAA GTTCTTTAGG5400GTTGAACAAC AGCCGCAAAA TTTCTTTAAA GACACTTTCT ATTCCATTGT TCACATAACT5460TTGAACACCG CTAAAAAATT CTACCCCACC TATTCTAAAA CGCTTCACAA ATACAAATTG5520CTCAATGATG TGGATTTTGC GATTTCATTG GCTAAAAGAA TTTTGAAAGA AAATTTTGAT5580TATTACAAAC AAAAACCACC CCAATAAAAG CGTGTATCAA ATAGTAGAAA TGGCAGTAGG5640CGCTTACAAT GGGGGAATGA AACACAACTC CTAATGGCGC TTATGTGAAA AAGTTCCGCT5700GTGTCTATTC TCAGGTGCGC TACAACGAGT AGGGCATACT AATTTTACAA GCAATCTTGA5760TGATACACTT CTACGATCTT ATGAATTTAT AATAATATCA TTTGCATCAT TATCTTTTAA5820TTTTTCTAAA ATATATTTTG CTTCTTTTTG TTAAGATATT TTGTCTTTTT ATTTTTTGGA5880ATAGGCTTTT TGCCTTCCTT ATAAGGACAT ATAGCGTAAG AATTTTTCCA ATTCATACGA5940TTTTGGCATA GGTGCTGTAA AAATGGATTG ATGAATTTTA ACCTAGCGCA TGCAAACAAT6000CCACTAGAGC GATGTTTGGC AAATCAAAGA ACACACAAAT CACAGTTCGC TTGAACCCAC6060AGGCACTAAA GATTTATCAT AGCTCAATTC GCCTCTAGTT TTAGCGGCTT GCAAATCATC6120ATAGAATGTT GCTTGATTAG GCACTTTCCC TAATTTGTAT TTCTTGCTCA AGCTTGGACT6180TACCTTAATA AGCTCATCAG TGAAGAATGG ATCATCATAG TAAAGCGCCT TGTGGCACTT6240GATGGGTTTG AGCGTGTTTT CTAGAATGAT AAGCTCATCG CCCATAGTCA TCAATTCATC6300AGGGGTCATC AAAAACCGCT CCTTGTTGCT GATAGAAGTA TTGGTCTTAC CGGTATTATC6360ATCAATGCTT CGGCTCACAT CTTGTCTAGT GTATTTCCCT AACACCTTAG AAAGTTTTTC6420AAAATGTTCA TAGTAGTTAT CGTTGTTAAT CCCATAATAC ATATTCAAAG AAAGGTTGTC6480TAAAATAGTC TTAGCGCCAT TCCTACCATA ACCAAGTGGG GGGTCATTCT CTAGTTGTGC6540CTTACTTTGA AACACAAACG CAGGGCGCAT GTTGTATTCT GCCATAATCC CTACCGCTTT6600AACAAAGGTC TCTAAATAGC CACAAAGCGT AAATTCGTCC ATGAGCATTA AACAACTTCT6660TTTGCATTGT GGATCATGGA TTGGCAGAAT AAGGTTGCTA TAAATCATCA CATTGAAAAA6720CAGCTCTAAT ATCGGCCCAA CAATAGTGCT TTCTTTAGGA TTAGCGATCA CGCCAATACT6780CACTTCATCA ATCCTTAAAC GCCTGAAATC AAAATCATTA GCGCTCGTGA AATTTCTGAT6840CATGGCGTTA TTGTAGGGGG CAAACGCTGA AGTATAGACC CCTTGGACAG AGCTGTAAGT6900TTCTTTAGCA CCGCCCATTG TCTTGAAATT ATTCCACATG TTTCTAGTGG CAGGACTAAG6960CACTCTTATA TTATCGCCAC TTTTATCTTC TTCACCTCCA AAAAATTCCA TCAGAGACAC7020GACTTTTTCC ATGTTTGTTT CTTCATCAAT CAAGTTGATA CCGCTTGCCA TAGAACCTAT7080GAAAAACATC GTGGGGGTTT CAGGCATGAT GATTCTTTTT CTTTTGACAA ACTCAAGCCC7140CTTTTTAGTC CACATAAGAT CCCTATAAAT ATTGCAATTG ATGACAAAAA GGTTTCGCGC7200TTGATTGCTA AAAAAAGGAT CTTTTTCATT AGGTCTTTCA GGGAACACGA GCTTTGCTAA7260TCCAAAGATT TGAGTGGAAA AATCCCCCCC ACTAGCCACC ATGCCATGCC CTTTTAGGCG7320TGTGTCAATT TGAGAGAGTA TGTCTTCAGT CAAAACCACA TCATTACCAA AATCCACATA7380AGCGAAAGGA TTAAATCGGT GTGTTTTTAA GGAGAAAGGT TCATAGATGA ACACTTTTTG7440GTTGAAGCGT TTTTCTCTGA TTTTTCCGCA AGTTTCCATA GTGTCAGCCT TAGGGTCAAA7500
CACAACGATA TTTTGAGGAT AATTGATCAT ATTGGGCATG ATGAAACCCA CCCCCTTACC7560GCTTCTGGTA GGAGCAATCA AGCCAATGAA CGCCTGCCCT GCGTAAGCGA TAAAATCCCC7620CAAACCACGC CTGCCTACAA TAACCTCTCG TTTGTCAAAG GCGCGTTTTT TATTATTGGG7680TGTGATGAGT TTAGCTTTGA TCATTTTTTC TTCGCTCTCC CAACTCGCGC TACCAAAGAG7740ATCATCAACT TTTTTATTCG CTCCTATGTC TCTAGTCCGA GTTAAGTATT TTAAGAACCA7800CACAAAAAAG GTAATGAATA TATAAGAGCA TAAAATAGAA CTATATACGA CTAAGGATAT7860ATTGAACCCA TAAATCTTGA GTGAACTAAA ACAAAGCGCC TTAAAATAGT ATGCAGGAAA7920ATCTTGTATC GCATAGATCC ATATATCAAA AAGATCATCA TCACTCTCAG GCAATTTATT7980ATCATCATAA AAATTACCTA AGAGAATCAA GAAAAATAAC CCTGATGTGA TAAAAGAATA8040GGATAGTAAT AGTGTCGCTT GTAACCAAAA GCCAAAGGTT TTAACTCTTT CTTTCAGCCC8100ATCTATAATG ATTTCTTTAA AGTTTTTTTT CTTTTGAGGT TGGTTAGCTT TATCTTTAAA8160AGCTTTTTTT TGAGCTTTTA TGAATTTGTA GAGAAAAAAT AACGCTATTA CCCCTATGAA8220ACTAAAAATA ACAACCAACT TATAATCCTC TATGAAATAT AAGGTGTTAT ACAACAAGTC8280TTCCATTGTG CCTACTACCT ATGTTTGATA TAAAATTCAT CACACTGTTT GTGGTGGTTG8340ATATGGACAA TCATATCAAT TAAATCTTTA AAGCCCTCAA TGAGGCTTTC AAACTTGATA8400TTCCTTGCTG CGTTATTAGA CGAACTCATG TTGGCTAAAC GAATAAACGC TTCTTCACTG8460CTCCCTGCAT GTAGAGTGGT TAGCGTGCCT TTATGACCGC TACAAAGCAC ATTATAAAAA8520TCATATGCCT CACTGCTTCT GAGTTCTCCT AAAATGATTC TATCAGGTCG CATTCTCAAA8580CATGACTTTA AGCAATCAGC AGAGGTGATA TTCCCACCAA AAAAAAGCTG TGTGTAGTTC8640TTGTGGTGTT TGAATACAAT CTCTTCGGTG TCTTCAATAG ATATGATCCT TTCTTCTTTG8700GGGATAAACT CCATGATGCT TTTGATATAA GTCGTTTTAC CGCTTCCTGT GCCACCACAA8760ACAATCACAT TCTTACCAAT AGCAATACCA TCTTTAATCG CGCTGATCGC TTGTTCTTTG8820TTATCTAGTA GATTATAAAA ACCCTGCTCT TCAAAGAAGC TATGAGGATA GGTTGTTTTG8880CTAGGTATCC TTATGGATAT GGAAATGGTT TCATCATTAA CTGTAACAGG GGAAAGGACA8940ATTTGCACCC TTTCACCATT CGCTAAATTG CTGCTCAAAA TAGGATTTTC ATAGTTGTCT9000ATTGTTTTTT TCTTAAAACT CGCACAACAC CGAGCAAAAT GCATTAAACG AGACAGACTA9060AAGGCTTTCT TGTCTCTCAC ATCAAATGGT TGCCATTCGC CATTATTTTT TAAAACCCAT9120ACAACCTTGT TCCCATTGTA ACAAATCTCA GTGATATTTT CCATTTTTAA AAAATCACCA9180AAAAGTTCTT CAGTAGCATG CCTTAGAGGA TTTAATGCCG CTTCTTTTAA AGCTCTTTCT9240ACTTCTAGAA ATTTTTTATC TTCTGCACTC AATCTGTCTT CAGTCATATT CTTATTCCAC9300ATTTAATTTT AATTGGGTTA TCAATTTAGC TCTTTCTAAA GAAGTGAGAT AAGTCTTATT9360TTTGCTCAAA ATAATATATT TAGTCCCTAT ATCTTGGACA ATCTCATTGA GCTTCCTTCC9420AAAATCATCT ATCCCTAGAA TCTGAAACGC CACGCCCATC ATATTGCCAT CTATGAATGG9480CGGTAAATAT TCTCTTGGCA GATAAGAAGC CACTAGGTCT GAAATTCTTG CTACTTCATC9540ACACATAAAA TTATTGAAGT TGTCAGAAAT TTCTGCAACA AACTCCCCAA GCGTTAGTTC9600ATTTTTGATT GTGCTTTCTG CAAAAGTCTT TTCTTGTTTG CAACGATTAT AACTGGCTAA9660CACAAGGTTG TTCAAATAGT TGTAATAAGC ATCAAAACTA ATAAAATTCT TTTGCATATT9720GATTCTTAAC TCTTGAACTC TTCTCAAAAA ATCTTGCGGA TCGTTGCTAT CTTTTAAAGA9780AGTGGCGTAA TTTTGAATAA ACTTATCCCT TACTTCATTT GCATTTCCCA TAAGAGATCT9840GTTGCTATCT AAGATTTTTT GTCTTTCTGT TTCATTGAAA CCGAGTTCCA TTGCTGTTTC9900CTTTCATTGT TTTTGCTTGG TTTTTTCCGC CACTACATTC TCTAGAAGAG CCTGTTGTTG9960GCTTTGTCTT TTATCTTGTA AAGAACCAAG TTTGGTAGCA TAGATATATT TTATCCTTTC10020TTTTGAATTA ATTGCCACCT TTGGGGCTTG TGGTGATTTC TTCATGCTCT CTAGACAAAG10080TTTTAGTGCT TTGTTTGATA ATTTCATCCA CCACAGATTT GTTGGTAATT TTAACATCAT10140ACACGCCACT AAAATCAATA TCGTCCATTG TGAGAATCTT AATACTATCG CCCTCATTTT10200TGTAAAAACT TGGGGGGATA TTCATCAGTT GCCCTAGAAT TTGATTAGAC ATCTGAGCTG10260
AACTTTGCAT ACTACCATTG ATAGCTTGAC CCAAAGCGTA ATTAAATTCA GGTGTCCTTT10320CACTTCTACC TTTGCCAAGG CCTATGAGTT TATCTAGAGC TATGATAGGT GCAGTTTGCA10380AGAAGCTATT AACCACGCTT GCTATCACAG CAAAGCCTAT GCGCTTCATG AAGTGATTGT10440TCACATAGCC ATCTACCCCT GCTTCACCCA GCATGCCTGC TGCTTGAGCG TTTGCTAGAG10500GTATCACCAC ACCATCAGGC GTAATGGCTT TAGTAAAGAC TATCATTAAG CGTGTCATAA10560TGGGTGTGCC ACCTTTCACG CTTTGATAAT TCCCATACAC CTTAGTGCCT TTGTCTAGTA10620AGATCATAGT GCCGTTCATG TTCCATACAT CTTTGGCTAC AACCCCACTC ACTATACCTG10680TGAGAGTGGC ATCTACTTTA GAAGTCAGAG TGATTTCAAT AGGGGTGTAT TGCGCTAAAA10740CAAATGTGGG ATCACTCTTG CCTATAAAGG CCTGTTTGAC AGGGCTTGTT TCATCTTGTG10800TTTCTGTTTT CTTTTTGTCA TCAACGGAAT TACCATTTTC ATCTACAAAT TCCCCACTCT10860TTTCTTTTTT GTTCTTAAGA GCGATTTCAC TGATATTCTT GGCAGTTTCT GCGACATCTT10920TGTCTATCTT ATTGCTTTCT ACTCTAGCTT CTGCTGTGGA TTTTTTACTT TTTTCTTTAT10980CGTCATCTTT TTTATTGCCA CCTAAAGCCT TAGCTAATTT AGCTTCTAAA TTCTTATCCT11040TAATGGTTTT TTCTGTTTCG TATTGCTTAG TAATATCAGG CTCTATGGAA GCATAAATAG11100GATTATCGCT AGCTATTTTG TCCGCATCAA CATTAGTGGC GTTAATAGTG GCAATATCGC11160CGCCATTTTT GAAATCCATT GGCAACAATG GATAACCTTT AGCCGCCATG TTGTCAAAGG11220TTTTGCGGTT TCTTAGATCG CTATAAATCA GATCCACTTC ATCAGCTTGT CGTTTAATTC11280CTAGAATTAG CGCCCTTTCA CTATCGCTCA AGCCCTCTAA ACATTGCTCT ATGGCTTCTT11340GATCAGTAGG GTCATCTAAG TTATCCAAGC ACTCGCTTGC TTGATGCAAT CTTTCTGTTT11400TACTCAATTG ATTTTGTTTG CTTTGTGTCC TTTTATTTTG GATTTCTTGG ATCAAATCGC11460TATAAAGGTT TTGACATTTC CTTTTTTCTT CATCGGTCCT AGCCGTTTTC AAACAATCCA11520AGACAGCCTT TTCTCTAGCT TCTTGCAGGT ATTTGAGCTT CTCTTCATCG CTCAAACCAT11580CCAAACACTT CATAATAGCC GCTCTGTCGT TAGGATCGGC GTTTTTCAGC ATCTTTGATC11640GCTTTAC 11647<210>120<211>26344<212>DNA<213>H.pylori<400>120TGCGACATCT TTGTCTATCT TATTGCTTTC TACTCTAGCT TCTGCTGTGG ATTTTTTACT60TTTTTCTTTA TCGTCATCTT TTTTATTGCC ACCTAAAGCC TTAGCTAATT TAGCTTCTAA120ATTCTTATCC TTAATGGTTT TTTCTGTTTC GTATTGCTTA GTAATATCAG GCTCTATGGA180AGCATAAATA GGATTATCGC TAGCTATTTT GTCCGCATCA ACATTAGTGG CGTTAATAGT240GGCAATATCG CCGCCATTTT TGAAATCCAT TGGCAACAAT GGATAACCTT TAGCCGCCAT300GTTGTCAAAG GTTTTGCGGT TTCTTAGATC GCTATAAATC AGATCCACTT CATCAGCTTG360TCGTTTAATT CCTAGAATTA GCGCCCTTTC ACTATCGCTC AAGCCCTCTA AACATTGCTC420TATGGCTTCT TGATCAGTAG GGTCATCTAA GTTATCCAAG CACTCGCTTG CTTGATGCAA480TCTTTCTGTT TTACTCAATT GATTTTGTTT GCTTTGTGTC CTTTTATTTT GGATTTCTTG540GATCAAATCG CTATAAAGGT TTTGACATTT CCTTTTTTCT TCATCGGTCC TAGCCGTTTT600CAAACAATCC AAGACAGCCT TTTCTCTAGC TTCTTGCAGG TATTTGAGCT TCTCTTCATC660GCTCAAACCA TCCAAACACT TCATAATAGC CGCTCTGTCG TTAGGATCGG CGTTTTTCAA720GCAATCTTTG ATCGCTTTAT CTTTTTGTTG GAGTTCTTTC GCTAAAAATT TTCTCGCTTC780
AGGGGTGAGT AATTTCTCGC ATTCTTGTTT CTCTTTTTCA TTCCTAGCTC TTGAAACGCA840GTCCAAATAA GCCTTAACGC TCTTCTTAAC TTCTTGCTCT AAGAGTTTTC TCGCTTCAGG900CGTGAGTAAC TTCTCACAAG CTCTCCTTTC TTCTTCATTT CTAGCTTGAG AGAGGCAGTC960TTTATAAGCT TTAAGACTCT CTTTAGCTAA AACATTTTTC TGTAAGTCTT TAAGGATATT1020TTTAAGACAT GCTTTTCTTT CTTCTTCATT TCTAGCTTTT TCAAACAACT TAGCACTTGT1080TGCTCTAAAA ATTTTCTCGC TTCATGCGTG AGTAATTTCT CGCATTCTTT TTTCTCTTTT1140TCATTCTAAG CTTTTGATAC GCCATCCTTG TAAGCTTGNA CGCTCAAATC AGCTAGTAGT1200TCTTTTTGCA AGTCTTGGGG AATATTTTTC AAGCATTCGT NTCGTTCTTC ATCGGTTTAA1260GCGTTTTTCA AACAATCTAG CGCTTGTTGT TTCAATCTTT CTATAGCTTC TTTAGACAAG1320CCTTTCAAAC ATTCGTTTTT CTCAGCTTCT GTTTTGGCGT TTTTGATACA ATCCTTATAC1380TCTTGAATCT CTTTTTGAAT CCCTAATTCC TTACGGAATT TCTCTCTAAT CTCAGGGTCA1440TTTATGAGTT TTAGGCACTC GTTTCGTTCT TCATCGGTTT TAGCGTTTTT CAAACAATCT1500AGCGCCACTT GAACTTTTTG TTGGTTCAGT AAGCTTTTTT TTAGGTTTTC ATCTTTGATT1560AAATCTAAAC ACTTGATCCT TTCTTCTTCA GTCTTGGCAT TTTTGATGCA GTCATTATAA1620GCCTCTAAAG TCTTTTTCAT TTGATCTTGA AGTTTTTTGT CTTTGATAAG CTTCAAACAT1680TCTTCATAGT TGCCACCATT ACTAATACAT TCATAAAAGG CCCTCAATGG GTTTTTGTCC1740TCAATTTCTG CAATATTCAA ATAGTTGTAT AGGGTTCTAT TGGGATCGTC ATTGAGGAAA1800AGATTCTTAT CGATCATATT GCCTTTTTCA TTCCGTTCTT TCAGCAATCG GTTATACTCT1860TGCCTTATTT GGATTTCATC ATTGACATAA AGATTCCTGT CTTTGCTAAA ACGAGAGCTT1920TTATCTTCCA AAGGTATGAA GTAGTGAAAA ATGCTTCTAG AAAATAAAAT AATCACAATA1980AGAACAGCGA CTACAATGCC ACCAATAATG TATTTCTTTT TGCTTCCTTT GATAATCTCT2040TGATCGTTAG AATCGTTAGT TATTTCTTCT GACTCGTATT CATCAAAATT GGTTTTAGTT2100TCTGTGGGGT TGTCAAGATG ATGATCTGAG CTTTCTTCTT TTGAAGAATC TTCAAAGTGA2160TTGGCTTCTG TTGCTTCTTC ATTGGATAAA TCTTGGGGTG AATGTTGTTG GGTTTTTTTA2220GAAGTTTCAT CATCAATTAA GTCTATTTGA GTTTCTTCTT GATACCCATT ATTTTCTTGA2280TCGTCTTCTT CGTTCGGTTC TTGGATTTCT TCATCTAAAT TCTTTTGCTG AGTGAAAGGT2340CTTTTATCAT TAACTAATTT TCTGGCTTTC TTGAATAACT TGTCTGCTAG ACTGCCATTG2400CTAGATTCTG AAGTTTCATT ACCTCCAGAA TCCATTTGAG TTTGGGTTTC TTCTAGCTCG2460TATTCATCAA AATTGGTTTT AGTTTCTGTG GGGTTGTCAA GATGATGATC TGAACTTTCT2520TTGGATTCTT TTGAAGAATC TTCAAAGTGA TTGGCTTCTG TTGTTTCTTC ATTGGATAAA2580TCTTGGGGTG AATGTTGTTG GGTTTTTTTA GAAGTTTCAA ATTTATCGTT TTCTTCATTC2640ATGTCTTAAC GCCTTTTTAT TTATCTCTGA CAAGAGGGAG CTTTTTAATC ACACGCTCCA2700ATTCACCATA GTTTTTGATA TTGTAATTTT TTGTCAATGG ATTTTTCCCA TAGCCTTTAT2760TTATTACTGT TACAAGGGCT TTGTCTTTAA TGAGCTTAAA CTTTTCTGCA ATTTCATTAA2820CTCTATACCA TCTCAATCCT GAATTGGTCA TGTTAGGATC AATGGCAGCA TCAGTCATGC2880TCAATTTCCC ATCAGGTTGA ACCACAAAAA TAGCAGGTTG GAGAGTGATG TTTTTGAAAC2940CAAAATAAGT GAATGTGCCA TCATCAAAAA TTTCAGAGGG CATAATATGC TTAGAGTGTT3000TTTCAGGCGC TTGGTAGTAA TTGTAGTTTC TAGGCACAGG GTTTCTTTTT AATGCATTAT3060GCACATATTG AGTCTCTAGC GCCTTTGCTT GATCTAAAAT AATTTTTTGT TTTTCTTCTC3120TTATTTTTTC TTTGTTGATA ATCTGTTCAT TGCTCGCCAT CATTACTCTA TTGATGTAGG3180CTGTGGTATT AAGATTTTCT TGTTTGATCA ATTCTCTCTG CCTTTTTGCT TCTTCTCTCT3240TTTTTAACTC CTCTTCAATA ACGCTAGAGA CTTCGTGTCT TTGTGGGTAT TCTAATTTGA3300CTGTTAGATA CGCCGAAGCA AAATTGTCTT TTTGAGCTAT TCTCAAAATG AATTGATACA3360AGGCTTTATT AGTCCGCACA ACAAGATTAG TTCTCCAAGC GCTATCGCTA GGAGATAATT3420CTATGGAGTT ATCCTCAGGG CTTTTTTGAG ACTTATCTGT CTTAATATTG ATTTTATCCT3480TGGCTCTTTG TTTCACTGCC TCTTCAGCTT GTTTTTTGTT GAGTTCTTCA ATTTGTTTGA3540
GTGCATTAGC TTGAGCCTGT TCTTGCATGT CCTCTAGTCG TTCCATTTGG TCTAATTCAT3600TTTCTCGTTG TTGCTTGATA AATTCGCTAA GATTTTTGTT ATTGCTCAAA TTTTGTGGGT3660TACTCATAGC GTTAGTGAGA TTTTCTAAAT TGGCTCTATT TTTTGCACGC TCTTCCTTTC3720TTTTTTCTCT TTTATCTTTT TGCGCTTTTT GCGCCTGTTC TTTAGCTTCT TTTTCTTTTT3780CTAGAGCTTT TTTTTGTTCT TCTAGTTCTT TAGGGTCAGG CGCATCTACG ATAAGTTTTT3840TTGTTTTTAA AAATTCTTGG TAATCTCTTG TCATTAGGGC AAAATTCACT GCTTCTTTTT3900CAAACATGAG ATTACTTTTT ACCGATTTAG GTTGAATGAA TATATGATTA GAATTAGGCA3960CAATACTCCA ACCTTTATTG AAACCTGTTG TGATGTAAGA AATAGTTTCA TCTTTTTCAA4020GTTGGATCAC AGTAACATTG TCTAATGAAG TCCAAATCGT AATAGGTTTT TCATCTCCCA4080AATAAGCAAT CTTCTTATTC ACCACTTTCA CCCTACCACG ATTAAAATTT TTAATGTCAG4140GTGCTGCTTC TATTACGCTA GATAAAAATA AATAACCAAG ACAGAAACAG CCAACAATTT4200TTTTAAAAAA TGCCTGCTCC ATCAACAATT CCTCTTAAAA AATATTTGTA ATTGTAACAC4260AAGAATAAAG TGGCTTATCC TTTAAACATA GATCCACCAA CTCTTCCCAC CATTAAGGCA4320TCTCTTTCTC TATCCATTCT GCTACCATCC ATAGATACAG AGCTAAAAAT GGTATTAACG4380ATCGCATTGA CACTCTCAAT AATCGTCTCT ATGAAGCCTT TTAAAATAAA TAAAGTGATC4440AAACTCGCAA TAGTTGAATC GATACCCAGT CCACCTTGAT TGATGTTTTG TATAGCAACA4500GAGGGCGTTA TGGTCAAATT GAATGCCAAT AATGCCAAAA CACCAAAGAG CAAAATAGGA4560ATAGCTAGAA CCAAGATAAA CAAATTATTA ATAAACCATA TCAATATGTT TTTCATAGAA4620TCTTTGAACC AATCTAGCAC GATCAAACAT ACCGCAATAG GGAACACAAT ACCGCTAAAA4680GCCCCCGCTA TCAAAGGTTT CACAACAACA GTGCTTATTC TCATAATGAT AGTTACTTGC4740ATAAAAATAG CGAATAAAGA AATACCTGCC ACTTGCAAGC CTTGTAACAA ACTAGGAGCG4800TTGTTGCCAA CATTAGAAAA AACAGCTCTT ACCGCAGGTA AAATTGAATT AACAGTGTAA4860TTCAGCACTA AATCTAAAGC CCAAAAAAGA ACTTGATTCA GCCCAAAAGC GCCTGCTAAA4920CCAAAATAAA TGGAATAAGA GATTTTATAA AGATAACCAA AACCTGCATA GGCGATAAAA4980GAGACCATCG CCATTTTGAA TAATGTCCCC CCAACAAATC GCATGTATTC AAAACCTATG5040TTAATCTGAA TGCCATTTGA AATATCTCTC AATTTCATAA AAGAAATAAA AACAAAAATC5100CCAAAAACAA TATAAATCAG GTAAGAAACA AAATCAAGAT ATGACCCTTG CCCTATGATA5160ATATCAGGCA GATTGACTAC ACCATATGCA GGAAGAACAG CAGCGTCCGC TCCTTTGACA5220ACAGATTCCA GTGCGGCAAA AGCAAGCATG GCTGTAGCGT TGTTTTGCAG TTTATTTGCA5280GTGAATTCTT TGCCAATCAG CCAAATTAAA CCATCAGCTG TAATATTCGT AGAAGCTTGA5340GCGGTTATTG AGCTAATTAT TTGTAAAAGA ACATTGCCTT TCACATTGTC CTCAATACCG5400CCTTTGGTAA AATTCCGAAT CTTGTTGCTC AAATTGTCTA GCACGCTATC CATGGATACA5460TCTATGTTTT TATTTATCAA TGCGTTCCAA CCAAAATTAC AGCCACTTGA GGGCGTTAGA5520GCAAAACCTT GTATAGCTGT AGCAACACTT GCCCACGCCG ATCCCCATAA AGGACCTAAA5580GTGTTCAAAT ATTTCACCAG ATTGCTAGCG GCTAAACTTG TGGCTAATGG TGTGCTAAGC5640GCTTCAATCA CACCCTGCCC TGAACTCTTT CCACTCCCTG ATGCACCAAT AGATGTATAG5700CCTGTCAAAA TATCTGCAAA ACTTTTGTTG GTTAAAACAT AACTCGGTAT CAGCATGCAA5760TAAACTGTTT GGTAAATTTT GAGATTTGAA ACAATGGGCA AATCAATAGC TTTCAACCAA5820TTAGGAACGA TGAGAAAAAA ACTTATGATC GTTATTAAAA AAGTCCTTTT AATATTAAAC5880ATGCTCTTAT TGTTTTAATG CCTTATTTTT TGATTCCACT TCTTTAATCT TTCTCAAAAT5940TTCGCTTACC GTCTGTTCGT TTTGTAAATC TGTGATTTGA ATGTCAAACA CTTTGAAGCC6000AAAAGGATTG ATGATAAGAT TTTCTTGAGA AGAGTTACCT CTGGCAAAAT CATAATAAAT6060AGTTACTTGT TTTTTAGTGA TATATTCATA ATTTTCCATT GTATCAGGTG TGATTTTGAT6120GGTAATGAAA AATGTTAATC TCGTTAAGGG ACTATTTTTG ACTTTTTCTC TTTGTATGTT6180AGAGCTAATG ATAGCTTCTG CTCGGACTTT ATCTACGAAT TGTCTGATAT TTTCATTGAA6240CATTCTCATT GCTTGGGTTT GGAAACTCAC ATCGCAATAC TGCATTAGTT GATCCTTGCG6300
ATCCCTCAAA GAATTTTTGC TATAACCAAA CAGCAATGAA ACAAATTTTG AAGTTGCACT6360ATCCACAACG GCTTCAGAAT TGACGATTTG CCTAGCATCG GAGCGTTTGA CAATTTTAAA6420TTCTCCTGTG TATCGATCAA TGCCATAAAC AAATATATCT GTTTTCTTCA AAGGCATCAT6480CATCACAATA CTCGTTACAG CGGCGACATT GAGTGCCATA GAGAGATAAA AAGCCCTTTT6540GAAAGTCCTA TTGGCCTTAT TGAGTTTTGC CAATCTATCA AGGGCTATCA CACCCCCAAC6600CAAATTTTCA TCAACCAAGA CTTCCTCGTT TTTTTTCCCT AACATGCGAC AACTTTATTA6660TTTAGTTATT GGTTCGTTGG TTGTAAAACT TGAGAAATAC TTGAGAAATT TTTTCATCAA6720AAACTCTTTA AAACAATTTC AGTTGTTATA GTATAGCAAA ATTTCTGTAA AAATATATAT6780TTGAAAAAAC CTTTAACAAA AACCTTTTTG GATAAGGCGT TGAACAAAGA TTCCTTATAA6840TGAATGTAAG TAGCGGATTG CTTCAAACAA GAAGCAAGAT GAAACACGAT ACAACTCAAA6900AAGTAAGAGC AATGAGTAAG CAGTCGTAAC TCAGTAATGA AATTTTAGAA GCGCTTAATT6960CAAAGAAATA AGACAATCAC AACTCAGAGA GAATGGTATA ATAACAAAAC AATTTCTCAA7020TTTAATTCTT GAAAGGAGTT TTATGAACGA TACAACAGAG CATCATGGAT CCAATTCGCT7080AAACGCCCCA TCACCTAGCA ACTCACAGAG CAATGATCTT TTAAATTTGC TAGACTCGTT7140ATATCCCAAA GGGAGTTTAG GGGAACAAAG ATTTCACGAA GCTTTAAAGA ATCAAGAAGA7200GTTGAAAAAT ATCCTGATAG AAATAGAAAA GCTACCGCAA GAAAAAAGGT ATGAACTTCT7260GATGCAGATA GGACAAGCCA AACAGAGAAT AATGGAGGCA TACGCTCATT CATTCTTAGG7320ATATATAGGG GGGTTAGAGC ATCTGTTAGG ATTGTGTATG GGTGGGATAT TTGTTTTGTT7380TGCAATCTAT TTTGTATTTT TAAGAACTAG CAAAAATGTG GATTTGGTGG AAAGTCTAAA7440AACAAAACTA AAACTTCAGT ATTTTTACTA TGCCTTTGGT GTAGGTGCGG TTTTGTTTTT7500TGGATTAGAG ACAATTAGAT CTATTTATGA ACTGTATATA TTAGGAATTG GTAGCACTAA7560CGATAAGGTG CTCTTTGTTT TGAAAAACAT TTGCTTCATA GGCATGGGCT ATTTGATTTA7620TAAAGTTATT AAGGTTATTG GTATAAAAAA TTTTATCAAT GGTCTTTTCA CTTCAAAGAA7680ACAAGAGCAA TAAGGAGAAA CAACAATGAA ACTGAGAGCA AGTGTTTTAA TCGGTGCGAC7740AATTCTGTGC TTAATTTTAA GTGCGTGCAG TAATTATGCG AAAAAAGTGG TGAAACAAAA7800GAACCATGTT TATACGCCTG TGTATAATGA ACTGATAGAG AAGTATAGTG AGATACCCTT7860AAATGACAAG CTCAAAGACA CACCATTCAT GGTGCAAGTG AAGTTGCCAA ATTACAAGGA7920CTATTTGTTG GATAATAAAC AAGTTGTACT AACTTTCAAA CTTGTTCATC ATTCTAAAAA7980GATTACGCTC ATAGGCGATG CCAATAAGAT CCTCCAATAC AAGAATTATT TCCAAGCTAA8040CGGGGCAAGA TCTGACATTG ATTTTTACTT GCAACCCACT TTGAATCAAA AGGGTGTGGT8100GATGATAGCG AGTAACTACA ATGATAATCC TAACAACAAA GAAAAACCAC AGACCTTTGA8160TGTGTTGCAA GGAAGTCAGC CAATGCTAGG AGCTAACACA AAAAACTTGC ATGGCTATGA8220TATGAGTGGA GTAAACAACA AGCAAGTGAT CAATGAAGTG GCAAGAGAAA AAGCTCAGCT8280AGAAAAAATC AATCAGTATT ACAAAACTCT CTTACAAGAC AAGGAACAAG AATATACCAC8340TAGGAAAAAT AACCAACGAG AAATTTTAGA AACATTGAGT AATCGTGCAG GTTATCAAAT8400GAGGCAGAAT GTGATTAGTT CTGAGATTTT TAAGAATGGC AACTTGAACA TGCAAGCCAA8460AGAAGAAGAA GTTAGGGAGA AGCTACAAGA AGAAAGAGAG AACGAATACT TGCGCAATCA8520AATCAGAAGT TTGCTCAGTG GTAAGTGATT GGAAGAAAAA AGGGAGAGAG TATAGGGTAG8580AGAACAACGC CAATAGGCGT TAGCCTTACT TGATAGGTAA GGCGATCAAG TAGGTAATCT8640TTTTAAAGCG CTCATCATTT TTTAATTCTG TCCCTGCTTG AGACAAATAT TTTTCAAACT8700GAATTTTGTT AGTTGCACCG AGATTTTTTA TTCGTTTTCC ACATCAAGCG TTTTGCACTC8760CTTTTTCTTT CATGTATTTT TCAAGATCAA ATTTCATTAG CAAATCCCTA ACATAGTCGT8820TATATTGTTC AATGGTTTTT AGATTAGCAT AGAAATTCAC TTCCTCATCT GTCATCACAA8880ACAATTTCAA TAGTGGGAGT TTAGTGCCAT ACAATTCTAT TTGCGAGTCC ATTTCTCTAA8940TACTCATCTT GAACATGATG GGCTTACTGA AGAAAGGCAG AAATATTCCA AGAATTGATC9000GATCAAATTT AGAGAATAAG AAATTAGGAT TCTCTGCAAT CGCATGCAAC AGCTCCATAG9060
TCTCACTCTC AAGCAATCGA TAAACCTTTT TAAATTTATC AACACCAATG CTTTTGATAA9120CAGCGCGATA ATCTTTCATG TAATTTCTTA GAACCTTATC TAGCGCTCCC TTGTTCGTAT9180GCATCTGTTT GAAAAAATCA TGCATGGTTT TTATTTGATC TATGATTTTC TTTTTTTCAT9240CAGTGTTTAA AAGTTCGTTC TCCTGCAAAC TCTCAATAAG TTTTTCTTTC TTATCTTTTG9300AGTCAGCAAT CATTGAGAAG AGTTTTCGCA TGTTGTTACT CATATCGCTC TTGGTCCCTT9360CAATGGATTT GGTTTCTCTT TAGATTGTTC AAATCGTAAA GTTTTATATT AAAATTATAC9420AATATCTGTT GTGTGAAAAT TTCAGAGTGG TCATAATTCA AAGAGCAAGA AACTATTTTT9480TAACCATAAA ATATTGTTTC AATCACTCTT ATATCTATTT TTCAAAACCT ACAAAAAACG9540CTTTCACAAA TAGCCCTAAA AACCGATTTA AAAAAGTTTT AATATTATAT ATTATTTTTA9600AAAAGCAAGA TCAGTTGTAA AAATGCGTTA CAACTAAAAT AAAGAGTCAA GATAACTCAT9660TTTCAAAAAG GAGTCTTAAG TAATAAAATC ATAATGTTCA GCTAGTAATC TATTGCCTCG9720TTGATCAAAC AAAGCTCTGC GTGAAAGATG AAAAAATTTC ACCTTTAGAT AGTTAATACA9780CCACTACAGT CTTACTTGAG AGACACTCAT TTTATTAGCG GTTTTGTCTG ATTTGCTGCT9840ACCAAAACCA TTACCAACCA AAGCAGATCC CATGTTTTTG ATACTATCGA ATCCATTCTT9900CAGCACTTCT GCCATAAAAT TCTTGATATT GTCCATAGGC AAGTTAAATT TTTTCCCTAA9960TGCTTCATTA AGTCCCATCA TTAACATCAG AAAGAACAAA AAATTTAATA TCATAGAAAA10020CAAATCACTG GATAAACCTG TAAAAAGATT TGTTCCGCCA CCCAACAAAG AAGCTAAAAT10080TTTTCCCATG ATCAGTCCTT TTATTTTTGG TTGTGTAAGT TCTTGCTTGT TCGGATCTCT10140AATGCGTGTT TTAGTAGGAA GCATTTCACA ATAGCATACC TAAAGCTACT AAGAAAATTC10200TTGAATCTAT TGGTAAGATT ACTCATGAAA TCAAGCGATA AGTAGCCACC AATCGCAAAC10260AAATCAAATA TTTTGCCACC AAACAAGCCA TATCCTTTTT GTTTTTATCT CCTAATTATA10320GCAAATTTTT ATCAATATTA ATTTGGAAAA CCACCACCAT ATCAAAAACA AATTACTAAC10380ACACTAGATG TAGAATTATT TTTAAAAAAC GCTCACTTAA ATTTAAAATC ATGGGGTTTT10440AGGATTTGAA TACCAAAAAT AGATTGGTTT TTTCAAATAA GCTAGCTTTG TGTATGTGCT10500TAAAAAGATT TTAGTTTTTA GTCAGTAAGG TTTTATGCTA ATGTTTGGAA ATAAAGAAAT10560TTCTCTAAAT CAAGTCTTGA GAAATTTTTG AACGAATCAT AAGAACCAAT TTTGCCATTG10620AGTCATAAGC ATGATTAGCT TCATTGTGAA GTTTGCGTGG CTTAAGAGAT AGTATTTGCT10680TATTATGCTG AGAGAAACGA GTAGTAAAAG ATAAGTAGTG TAACTAAAAA AGCTAGGTTT10740TATTATAAGA GCAAATAAGA ATAATATTGG ATAAACTAAA ATCACCCCTG CCCCATAAGA10800AAAAAGCCCT ATTAAAAAAC CTATGACGAT AGAGCTGATA TTGAACAGCC TATAATAAAG10860GCTGTACTTA TCTAAATGTT TGTTGAAAGA ATGTTTGAAT TGTAAGAAGT TTTGTTTTAA10920TTTGCTAATT GGCTGTTCCA TTTTGGTTTT TAAAGAAATA GTTCAAGGCG GTGAACCTAT10980AAAGGAGCAT AAAATAATAA ATATTTTACA AAACCACCCT AATCTAACTC CAAATCTCAA11040AGAATACCCC ACTTGCTGAT ACTAACATGT GGTATAGCAC AAACCAACGA CTTGTTTGTT11100TATGCTAAAC AAAGAACAGA AAACATCTGT TAAAGAATAA AGGAGTTTTC CATCTAAAAA11160CACAGATCCA TTGTATAAAA ATAATCTTAA GAGAAACTTA AAAAATACCA ACAAGCCGCA11220TACAAGCAAG AAAAACATAA CACTATAAGA CCTAGATTTT ACTTACCTTT TGGATATGGA11280AAAATTTTGA TCCATAGTTT TGTAAAAATT GTGGTAAAAT GTATTAATAT TTTTTGAAAT11340TTTAAGGTTA CAAAAACTAT AAGATGCTTG CAAAAATCGT TTTTGGCTCA TTGGTTGCGT11400TTGGAGTTTT GTCGGCTAAT GTGGAGCAGT TTGGTTCATT TTTCAACGAG ATAAAAAAAG11460AACAAGAAGA GGTGGCTGCA AAAGAAGACG CTCTTAAGGC TCGCAAGAAG CTCTTAAACA11520ATACGCATGA TTTCTTAGAA GACTTGATTT TTAGAAAACA AAAAATCAAA GAGCTTATGG11580ATCATAGAGC TAAAGTTCTT TTAGACTTAG AAAACAAATA CAAAAAAGAA AAAGAGGCTC11640TAGAGAAAGA GACAAGGGGT AAAATTCTTA CTGCTAAGTC AAAGGCTTAT GGCGATCTAG11700AGCAAGCCTT AAAAGATAAC CCTCTCTATA AGAAACTTCT TCCTAACCCT TATGCCTATG11760TTTTAAACCA AGAAACATTC ACCAAAGAAG ATAAGGAGCG TTTGAGTTAT TACTACCCTC11820
AAGTGAAAAC GAGCAGTATT TTTAAAAAAA CTACCGCTAC CACTAAAGAT AAGGCTCAGG 11880CTTTGCTTCA AATGGGTGTG TTTTCTTTAG ATGAAGAACA AAACAAAAAA GCGAGTCGAT 11940TAGCTTTATC TTACAAGCAA GCGATTGAAG AATATTCCAA TAACATTTCT AATTTATTGA 12000GCAGAAAAGA ATTGGATAAT ATAGATTATT ACTTACAGCT TGAAAGAAAC AAGTTTGACT 12060CCAAAGCAAA AGACATCGCT CAAAAAGCTA CTAACACGCT TATTTTTAAC TCGGAACGCT 12120TGGCGTTTAG CATGGCGATT GATAAGATCA ATGAAAAATA CTTAAGGGGC TATGAGGCTT 12180TTTCTAACTT ATTGAAAAAT GTCAAAGATG ATGTGGAATT GAATACTCTG ACTAAAAACT 12240TTACCAATCA AAAATTGAGT TTCGCTCAAA AACAAAAATT GTGTTTGTTG GTTTTAGACA 12300GCTTCAATTT TGATACCCAA TCCAAAAAAT CTATATTAAA AAAGACTAAT GAATACAATA 12360TTTTCGTAGA TAGCGATCCT ATGATGAGCG ACAAAACAAC TATGCAAAAA GAACACTACA 12420AGATATTTAA TTTCTTCAAA ACAGTGCTTT CTGCATACCG AAACAATGTT GCCAAGAATA 12480ATCCCTTTGA ATAGGAAAGG AGACGCTCTT GAAAAGTATC TTCAAAAAAC TAGGTTCTGT 12540CGCTCTTTAT TTTTTAGTTA TTTATGGGGG CTTAAACGCT ATCAATACAG CGTTATTACC 12600GAGTGAATAC AAAAAATTAG TGGCTTTAGG CTTTAAAAAA ATCAAAACAT TCCATCAAAG 12660ACATGACGAA AAAGAAGTTA CAGAAGAGGA AAAAGAATTC GCCACTAACG CTTTGAGAGA 12720AAAATTACGA AATGATAGGG CGAGAGCGGA GCAAATTCAA AAGAATATTG AAGCGTTTGA 12780AAAAAAGAAT AACTCTTCCA TTCAAAAAAA AGCGGCTAAA CACAAAGGAT TACAAGAATT 12840AAACGAAACT AACGCTAACC TTTTGAATGA CAACCCTAAT AGTAATTCTT CCACTGAAAC 12900CAAATCCAAT AAAGATGATA ACTTTGATGA GATGATCAAT AAGGTGAATG AAGCTTTTGT 12960GAAACCTGCT GCTCCGCTTG TGCCTAATGA GTGGAGAACA CCTGAAATTG AAATCATTAT 13020CAATGAGTGT ATTATTTCAA GCAACGATTA CGATGGGTTA AGAAAGTGTT TGATCAAAGA 13080CATCAAGGAT CAAAAAATTC TTGCCCCCTT ATTAGAAAAA ATTCAAGAAA TAGAGACAGA 13140AAATAACAAG TTTTCCAGAC AACACTTGAG TGGTTTAAAG CTCGCTCTTA ATAACAGCAA 13200CAATAGAACC TTTCTTATAG CTTCATGCGC TATTTGTGAG AAGAGAAAAA AAGAAATGGA 13260GCAAGAACAT AACTACCAAG ATACTACAAA TGCAAGTGAG TTTGGAGCTA CTGACACAAA 13320AGAAAATGAA GCAAAAGAAA ATGAAGCAAA AGATGCAGCA TTCTCAAACA ATCGTTCTAA 13380ATCTGAACTG CCCAATAGCG TCATTAATCA AATAGAACAA AGCATCGCTC ATGGAAAAAA 13440ATAGCGATCA AGATTATTAG CTCAAAAAAC AACTAGAGAA ACAAATCCCA AAGGTTAGAA 13500ATCATGGTCT ATTGTCTCAG AAAAATCATT TAACAATAAT CTTACTTGAT TGCCTTTCTT 13560GTAGGTATTG CCGCTTGCTT TGTTCTAGGG ATATTTCTAA TGCGTCCAAC TTTTCTAAAT 13620AATTTAAAAA GACCTTGTCG TGAGCTAACA TGAGCTTTCT GATTCCTTTA ATGAAATTTT 13680TATTCTTTAG GCTTTCTACA AGCGTCTGTG AAGCAGTGAT TAAGGAAGCT GTACCTCCAA 13740TGTTGCTCTG ATACGCCTTT AGAGAAGTTT CTAAACGCTC TCTTATATTT GGTTTTTCTG 13800GCTCGATTTT CAGCTTCCCC TCACAATAAA GAACTAAAAC TTTATCGGAT ATTCCGCATT 13860GCTGCTCAGC AGTCTTTTGA TCTAAGGGAT TGATTTTCAT ATAGGTTAAT AAAAGTTCAG 13920GGCTAGACAT ATAAGTTTTG AAAATCACAT CTTCTGAGAT GAAAAATAAC TCATTGGCTT 13980CAAAATTGGC TTTCAATAAC GCTAAATCCC CTCTCAAAGC AATGGCCGCT TTTTTGATGT 14040TTAGAGAATC TTCTTTACCT ATTGTATCAT TAGCGCTAGG GCTAGTGGTT GAAAAAATCT 14100CATCTAAGTT TTTGAGCACT TGTTGGTTGG TCTCTTTGTA GGTGCTATCA AGTTGCTTTA 14160AACCGCTTGT TATATCTTCT GCCATCAAAA CAGACAATAG CAAAAAAGAA TATATGGTAT 14220TTTTCACGAG TGTTTTCATT TGACAATAAC TTTAGAGCTA GCAATGTTTC TTGCTGTCGT 14280TTCTCTCTCT AATTTCAGTT GTTCTTCCCA AAGGTCGGCT TTTTTTTCAA GATTCTCTAT 14340ATAGTTTAAA TGATTTTCTG CGTTTAAGAT AGCAACTTCT ATGAGCGCAT TCAAATCTAC 14400TGATCCTTTT AAGGTTTTGA TTTCTTCATT GATCCCATTC AAATAAGCGA TATTTTGAAA 14460ATCTGCATCA TTTAGTTTAT TTTGAATAAG GGCTACAATC TTTCTGTAAT TCTGAATAAC 14520CTGTTCCATA AGGCATGCTG AAATTTTTAG CCCATCAAGA TAGGGGCATT TTGTGGGTGC 14580
TAGAGTAAAT GTTTCAATGA TTCCAAATGG TGCACCCATG CTTGAAAAAA AACTAAGAGC14640AGGCTCGTAG ATGGTGCTTT GAAACAAAGC CTGGCCTGTT AGGGAATTGT AGTCAATAAG14700GGTCGCTTTT TGCATAGCCA CTTTCAACCA TGTTTCAAAA CCTTTTAAGG TTTCTTCAAA14760CGCCTTGATA CCAATCGTAT TGTAAGCGAT GTATTTAGCG TTGTCAGAAG AACTTCCTAG14820AGCTTGAGAA AGCATTTCCA TTTGTGTTTT TAGGGTAACG CTCGGTTCAA AGCTGTTTTT14880TAACGCTTCT AAGAGAGCGT TTTGCTGGTT CATTTTGAGC TTGATCATTT CGTTATTTTT14940TTGGAGCGCA ATTTGCATGT TTTGGATTTC TGTTTGGGTA TTAATTTTTT GTTTTTCCAC15000GATCATGTTG ACATTTCCTC CCAATGCGCT AAGTGCGCTT GAATACCCTT CCATGACGCC15060AAGCAAGATG TCTGAACCTG CAAAAAACCC CCCTGTCATG CTATTGACAC CATTAATAAC15120GCCATTAGCC CCTTTTAACA TAGCGCTTAT AGTTGCAAGC TGAGTTCTCA ATTGTCCCTC15180TATTTGTGCT TGAATGGCTT TTTCTTTGGC ACTAGATTGA GCCTCTATGG CTTTTAATTC15240GGCCTGAGCG GTTTTTTGTT TGGCTTGTGC ATCCGCCTGA ATGGCTTTTA AGGCAGATTC15300AAGCGTTATT ACTGCCCCTG CACCATTCAG AAACAAACCA CAAAAAGTTA AGAAAGAAAA15360TATGCTTAAA AAACATTTCA CATCTCTTTC CTCACTTCAC GATTATTTTA GTTTGCACCC15420TTTCTGTTAA GTAGCTATCT TTTTGCCCCT TAAGTTTGTT TTTGATGTAA TCAAGATAAG15480TCAAATGCGA TTTCAAAAAA GATTTATTCG CTACTATATT GTAATTATAT AGCGAGCTTA15540TGTTAGAAAT CGCTTGAGTG TCATAGGTGC TAGTAGCTAA TCCTGATTGA TTAAGTATCA15600TTTGAGAAGC GTTCTGTAAC AAATTGGTGT TGTTTTTCAC AAACTCTATA TAGTATTCTC15660TCAAAATTTC TGCTACTTTT TCAGCATAGC AATAAACGGC AAGAACTTTG TCCCCAATAG15720GGCATGCAGG AGTGGTCATA GGATTAACGC CTGAAGTTAG GGCATTAGCG GCTAACGCTT15780GGTATTTGGC ATAAACAGTG GGCATAGAGA CACTCATAGG AGTCATAGAA ATTTGCATGC15840AACTAAAAAA CACTTTTGAT GAGCTAGCAA GCGCGCCTAA AGCGGTACAG CTATCAAGGG15900AATCGGCTGT ATCATTCATT GAGCTGTTGC TTGCTTGAGA ACGCAGTTGT TCTTGTAGGG15960CTAGGGCGTA TTTTGGTGCT GCACTTGTAA TATTGCCTAA GATACCGCTC ATTATTTCAA16020CCGTTGTTGG CACACTAGGA ACAGCGATTT GATTCGTCGC ATAAGCTTCA ATAGCACTAG16080GATTTTTAGG GGTCGTGTTA CTCGCTAAAA TGCTTGCAAT CTGACTATTA ACAGCACCAA16140TTTGCGCACC TTGCGTGTTG CCTTGCGCGT TAAATTCCCC TGTTAATTTG CTGATATTTA16200AGATATTGTT CCCCACAGCC ATGCTTTGAT CGTTAAAACC TTGATACAAT TGGTTGTATT16260GTTGGTTAGC GGCTTTCATA GGCATGCTTA CGGCTTCAGC GATGCTTTGA TTGTATTGGG16320TCATGATAGC GGTCATTTGC GGATTCGTAA ATCCCACAAC AATAGGAATA ATCGCTGCTG16380ACATAGCACC CGCTACTATT CCTGCAAATG GCCCTGCAAC ACCACTCGTG CTTGAGATGA16440TTGAGGAAAT TTCCGATAAG AAGCCTGCAG AAGATGATTC ATATATAGCT TGTGTGCCTG16500CCATGTTAAA ACCCCCCTAA TGGTGGTAAA AACGATGAAT CTGGGTATGT TGGTGCATAA16560CCATACATGA AAGGATTGTT TGGGCCGTAA TCGCCCATCA TTTGGCTCAT GAGAAGATTT16620TGAATGCCCC ACATCGCATT GATACCTAGA TTATCATTAG GTTGAAAACT CCCTAAACTT16680ATGTTATCAA ACTTGATATT AACATTCTTA TCATTATAAT CATTGAGTAT GGCCACTTTT16740TGCTCTAGGG TTTCTTTAGG GATCTCTATT TTTAGTTGAT CTCTAGAAAC AAGCCCCACG16800CTATTTAGTG CCATATCTTC AGGACTAATA TCTTTTATTT CAGTGTTTTG GTCAGCGTTA16860AGCGGAGCGT TAAACATGCC AATGATGAGA CACCAAGCAA ATAATAATTT AATTTTATAA16920AAATTCGTTT TCATACTTTT GACTCCTTTA TTCTTATTTT TAGCACTATT CTAGCGCATT16980AACACCGCTC AATCGCTATT TTTATTTTGA TTTTTTTGAT CGAGCATTTT GTTTGTTACT17040TCATCAATGT TTTGAAAATA TTTTTCAAAA AGCTCTTTCT TTTTAGCTTC AACGCTCATA17100TCAACCTGAA TCCAATTAGG AACAATAGAG TCCATGATTA AATGCGTGAA GTCATAGGCA17160TGATTGGTTG GGTATATTTT GTTTTGAACA TAGTATTCTA AAAAATTCGC TTGAACAAAA17220AAATTCTCTA TATCGCTCTG CATATCCTCG CTTATGTTGT TATTGATAGG TTTTTCTAAT17280AATCTGAGAA TCCTATACGA CTGCATGATA GTTTCTAGCA TGAAGTAAAC ATAAACATAA17340
ACTAATGAGA CAAAAGAATT ATTTGCTTTA AACGAGCTTG CGTCATTTTT CCCACTTTGA 17400AGAGGAATTA ATCTTGATAG TGTTTTTTGG GGATCTTGCC CATCGTTTAT TTCTTTAAAA 17460AAGCGGAAGT CTAAATCCTG ATTATTGAGA AATGACTTGA CAAAGTGAAG ATTAGCATTG 17520AGACTATCTA TGAGACCTGA ATAAAGGTGT TCTGTTTTGA CATCATCTAT ATTTAAAACA 17580TTCTCATAAA ACACATTGAC ATGGTCTTCT AAGAAATTAG AAAAATCATA AAGAGTGGTA 17640AGGTTTTGTT CAGTGATTTC GCCTTCCATT TCTTCTTCTA TGAAGTCCAA TTCTTCTCTC 17700AGTTCAAAAA GATAATTAGA AAAACTATCC AAAATCGTCA AGACATCATT TTCTAAAATT 17760TTCAATAATT TTTGTTCACG CAAACTTTGT TTCATTTTAA TACTCCTTTA TTTGTTGATA 17820CATCTGCCTC AAGGCCTGAT ATTTATCTAT GATACTATGG TTTTGGATAA TCTTGTCAAT 17880TTCTTTGACA AATACAGTAT CTGTGGATAA AATTTTCAAA TATTCTTTAG GAATGCCTCT 17940CAAATTAAAA CTAGCGATAA CGCTAGGGCT TCCATCTTGT TTGTAGAGAA TTTTCCTATC 18000TAACCCCTTA GTGATGATTT CAAATTCTTT TTCTGTAACA TTAGCCAATC TTTGGTAATC 18060AGAAAGATTT CCCCCATCGT TTCTCAAAAA AATCTTTGTA GGGCATTGTT CTCTAATAGT 18120ATCAGCAATA GGGCAAGCCA AAAGATCAGT GATGCTTTGA GTTGCAAGTC TAACAATAGC 18180GTTTCTTTTC CTTGCAGTTT TTAGCATGTC TCTTACAAAA TAAGCGACCT TTGGATCGCC 18240TAAATATTTC CAAGCTTCAT CAATATCTAA AACAAATCTA CGCCCATCCA TTGCCTCTTG 18300AATACGAGCG AAAAGGTAAA AACAAATAAA GGGCGAAACA TCATTATTGT CTAGGAAACT 18360TGACCCATCA ACGCCAATAA TCGTTTTTGA AAAATCTAAG CGATCGGTTG CTTTATTATC 18420AAAAAGCCAT TGAAATTCAC CATTGGTTGA TTTGCAAAAA GGCGCTAATC GCGCAACAAG 18480CCCATTAGGA TCATTGTGGT CTTTTCCGAA AGCATTAATC AGTTGAGTGA TAGGATAATC 18540TAGGTTCATA TCTCCTGTGA TAAGGTTGGT TACTGCCGCT GCAAGCGTAT TAGAATCGGC 18600TAGACTAAAA GAGATGCTGT TGCCATTTTC ATCTTTTTCA TCGCTTTTGG TTGCTAAGTT 18660TTTCACAAGT TCTTTGACAA CAGAAATAGC TGTTTGTTTT TGCTCCATTG TTGCATTGGA 18720TTTTTGCACA CAAGCCGCCC AAGCAAAAGG ATTTAATCCT GTATCTGTCC CTAGCTCAAT 18780CTTGACATAC TCCCCACCCA TTGCGACAAT ATTCCCATAA GCGCCATAAT CTTTATCCAT 18840ATAGACCATA GTGAGCTTTT GTCTGTCTTT GCTGACATTA GCAGGAAAAT TGTGAGCAAA 18900TTGTCCCATA GCGTTTAGAG TCATTGACAT AAACACCGTC TTACCTGAAC CGGTTGAGCC 18960AAGTATCAAA GTGTGTCCTG CTGAAGCTGA ACCAAAATCA GTGGGCATGT GGAAGTTCAG 19020ATAAAAAGGC GAATTGATCT CGCTTTTTAG CGTCATCACA CTATTGCCCC AAGCGTTATT 19080CTCTTGATTG CCATCAAAAC TCATAGCCCT CATCGCAATA AAATCAGCAA AATTATTAGA 19140AGTTACATCA AAAATAAAAG GAAGCGTGAT AAAAGAGCAA TGTTTGGCAA AAAAGTAATT 19200TTCCATAGAG AAAGTCGCTG CGTTGGCTAA AAAACCTTTA GCGTTAAGAC TAGAGACGCA 19260TTCCTTGACG CTTTGTTTCA TTTTTTCAAA GCTATCAGCA AACAACACTA AAGAATTACC 19320ATAACTGCCT AGCGTAATAT CACCATTACC CACTAATTCG CTCAAGCAAC CTAAAGTCAT 19380GCCCTGCTCT TTAGAGCCTC CACTAATAAT AATTCTTCTA GAGGTGAAAG CCAGCTTGTC 19440CTTTAAAACC TGTGAGTTTT TAGGCGAATA AGCATGCATA AAGATAAATT CGCTGTCTAG 19500AGCGTTGATC TTGTCAAACA AATCGCTCTG TGATTTAGGG GCGTATTCAC TAATCTCAAT 19560AGCGCTAAAA TATTTTTCAC TCAAATCGTC ATTTAAGATT TTTCCATGCT TGTTGGCAAA 19620ATAAACTTCT TTCACCCCAC CATGCATTTT TTCCTTGAGA TACAAGTCTT TTCTGTTGCA 19680AATAAAAGGG GCTTCATTCA TTCCTACAAG AAAATTGTAA AACTCGCATT GTTTGGAGTA 19740AATAATGCCA TCTTTGGTGT ATTCTTTTAA TCTAGTGGGG TGGTATTTAC TCAATAGCTC 19800TTCTATGAGC TCTATCCTAT CCTTGAAGTT TTCAAGCTTG GCTCTAATAA TCCTTTGAAA 19860CTCTTCAAAA TTATTGTCTG CAAAATGCTT TTTATTCATA ACGGGTTCAT TGAGAGTGTC 19920TAATAAATCT TGCTCTATGG TGAGATAAAA ACTAATATCA TAAAAACTTT CTCTCTTTTG 19980CTTCTCGTTA TAAGCTCGCA TAAAATCATT AGAAAAAATA AGACCATAGT CCCTATTGGT 20040TTCATCAATA ACGATTTTCT TTTTAACAGT GTGGAAATAA AATTTGAATT CAGGGGTAAC 20100
AAAATTCCTA AAAACGCTAT AAATAGAAGC GTGTAACTCT ATGAGATCTT TTTTGGAAGT20160GGTTAAAAAA TCAATGCCCC CCAATTTGAT TGTGCCTAAA AGAGAATAGT TGTTAGTAAG20220GATCACCCCA TCATCTAAAA AACATTCATA GTTATTCGCC AAATAGGAGT TTGCAGCACT20280CACAAGTCTG TCTTCTCTGT TTGGATTTAA GTGGATGTCA TTAGCCATTT CTTTACTAGG20340CTTCATGGAA AAAATGCTCA TGAACGCTTT GTTTTTCACG CCCTTAAACA AAAAAGGTTT20400TTTAAATTTC ATCGCTCGCT CCATTCTTTG ATAAAGCCTA TAATCTTTCT TGAATCCAAG20460AGCTACAAGC ACAATAACAA TCGCTACAAT CAAAACAGGT TCATAGGCTT GAAAAAGAAT20520AACAGATAAT ACAATTGTTA CAAACAATAT AAATATAGAG GAATAAATAA AAGTTTCAGG20580GAAACCAAAC AACCTATTCC CCCCATCAAA CAAGACTTTA AAAAAGGGAT TGACACCTTT20640TTGCATGTCT GCTTTAAGTT CTTCTATTTT TTGAAACTGC CGCTTTTGAA CCTCTTGCTC20700TATGATTAGC TTTTTTTGTT CATCAGCTTG CTTGCTTGCC ACAAACACCC CTCTCTTTAT20760AGATATATCG CTTCACATGT AATCGTATAA AAGATTTTTT TGAGAGACTC TATAGCGCTA20820ATATGTTTCA AAAGATCATT AGGGTCATAA GAATTGAATA CGGCCAATAA AACATTATAC20880AGCTTATCAT CGCATAGAAT TTCTCTTGTT TCTCCGTGCA ATGATAGGAA GCAGCGTTGT20940TTGTTGGTCG TGCTGATGCT TTTGAAAGTG AAAAAGTCTT TCACTTCAGG ATTGATCTGC21000AATTCCACAT TCAACCCCAT TTCTTTACCC TTTTCATCAA AGATTTTTTC AATAATTGGA21060TCGTAATGCT TCAAATCCTT TATTTTTTTA AGGACTCTAT TGACAATCAC GAAATCAAAA21120ACTTCATTTT TGATAATATC GGGATTGACT TCTTTGAAAG TTACTTTCTT GTCTTGCAAA21180TTTTTGATAG TCGCCTTGAA ACTATCAAAA TCTAAATTCG TATAAACACG CCCACTAGGA21240GTGTTTTTTT CTTTTTCTAG TGCTTCTTTT TTCGCTTGAT TATCATCATT TGCCAACCCA21300TACGAACTGA AAACAACGAG ACTTAAGAGA ACTTTCAAAA AAAAGTCTCT TATTTTTTTA21360TTACTCTTAT TGTTGATCAA TTTAGCTAGC TCCTCCACTT TCGCCAATAT TGAAGCCAAA21420CTTAGTGCTC AAATAGATAA TACCGCCTGC CACCGCTAAC ATAGCTATGG GCTGCGCATA21480AGCAAAAACA GTCGCCTGAC CTCTTTTGAT GTCATCAGAG ATTTTCCAAA TATCTGCTAT21540GCCTTTGACC CCTAGAGCGC AACCACCTAC GATCGCTAAA ACAGAAATGA TCTGAACAAC21600CAAACTTTTA GTCTCAGTAA CGCCTGTTGT AGGACTGGTG ACTGCCATTA AAGGATTGGT21660TGTTACCGCT AGCCCTAAAG TTACTACAAC TTTCTTGTAG CTGTCAGTGA TTTTTGTAAG21720AAATTTCATG CGTTTCCTTT CAGATTGAAA TAATGGTGTG ATTATACCAA AAAAAAATAT21780TTTTACACTT CCACGCAAGC ACCATTAGAT TAACTGGTCT TGTGTGTATC CTTCTTGATT21840GAGATAAAAA CCGACACTTT TGTTTGACAA TTGGCACAAT TTAGAACAAT AAACCTACTT21900GTCCCAACCT TTTTTGCTTC TCAAGTCGTT GTAGAATTGT AGATCTTTAG GATCTTTGAT21960GTATTTTTTA ATAATCTCAG GTTGGAACCT AAAAACAAGC AAAAACAAAC CCAAGCTGAT22020CAGAGTGAGA ATAAAGCTCC ATTTTAAGCA ACTCCATAGA CCACTAAAGA AACTTTTTTT22080GAAGCTGTCT TTGAAAATCT GTCCTATTGA TTTATTTTCC ATGTGGATCA CAAACACTTC22140TTGTTAAAAT TTGTCTATTT TAGCAAGTTT TTGTTAATTG CGGGTAAAAA AAATATGAAT22200CGTTCCTAGC CTTTAGACGC TTGCAACGAT TGGAATTTTT CAATATTAAA TAACATTAAT22260GAAAAAATGC CTAATATTGT GATATAATGA GAGCGTTCAA AGATATCAAT TGACCACTCA22320AGCGTGTGGT GGTTTTTAGC AGTCTTTGAC CAAATTTAGG TATAGTAAGG AGAAACAATG22380ACTAACGAAA CCATCGATCA AACAACAACA CCAGATCAAA CACCAAACCA AACGGATTTT22440GTTCCGCAAC GATTTATCAA TAATCTTCAA GTAGCTTTTA TTAAAGTTGA TAACGCTGTC22500GCTTCATTTG ATCCTGATCA AAAACCAATC GTTGATAAGA ATGATAGGGA TAACAGGCAA22560GCTTTTGAGA AAATCTCGCA ACTAAGGGAA GAATACGCCA ATAAAGCGAT CAAAAATCCT22620GCCAAAAAGA ATCAGTATTT TTCAGACTTT ATCAATAAGA GCAATGATTT GATCAACAAA22680GACAATCTCA TTGCTGTAGA TTCTTCCGTA GAGAGCTTTA AGAAATTTGG GGATCAGCGT22740TACCAAATTT TTACGAGTTG GGTGTCCCTT CAAAAAGATC CGTCTAAAAT CAACACCCAA22800CAAATCCGAA ATTTTATGGA AAATGTTATA CAACCCCCTA TCTCTGATGA TAAGGAAAAA22860
GCAGAGTTTT TGAGGTCTGC CAAACAATCT TTTGCAGGAA TTATCATAGG GAACCAAATC22920CGATCGGATG AAAAGTTCAT GGGCGTGTTT GATGAATCTT TGAAAGAAAG GCAAGAAGCA22980GAAAAAAATG CAGAGCCTAG TGGTGGGGAT TGGCTTGATA TTTTTTTATC ATTTGTATTT23040AACAAAAAAC AATCTTCCGA TCTCAAAGAA ACGCTCAATC AAGAGCCAAG GTCTGATTTT23100GAACAAAATT TAGCCACTAC CACCACCGAC ATACAAGGCT TACCGCCTGA AGCTAGAGAT23160TTGCTTGATG AAAGGGGTAA TTTTTTTAAA TTCACTCTTG GTGATGTGGA GATGTTGGAT23220GTTGAGGGAG TCGCTGACAA GGATCCCAAT TACAAGTTCA ATCAATTATT GATCCACAAT23280AACGCTTTAT CTTCTGTGCT AATGGGGGGT CATAGCAGCA TAGAACCTGA AAAAGTTTCA23340TTATTGTATG GGGATAATGG TGGTCCTGAA GCTAGGCATG ATTGGAACGC CACCGTTGGT23400TATAAAAACC AACAAGGCAG TAATGTGGCC ACACTCATTA ATGCGCATCT TAATAACGGC23460AGTGGGTTAA TCATAGCGGG TAATGAGGAT GGGATTAAAA ACCCTAGCTT CTATCTCTAT23520AAAGAAGATC AACTCACAGG TTTGAAACGA GCGTTGAGTC AAGAAGAGAT CCAAAACAAA23580GTGGATTTCA TGGAATTTCT CGCACGAAAC AACGCTAAAT TAGACAACTT GAGCGAGAAA23640GAGAAAGAAA AATTCCAAAC TGAGATTGAA AATTTCCAAA AAAACCGTAA GGCTTATTTG23700GATGCCCTAG GGAATGATCA CATTGCTTTT GTTTCTAAAA AAGACCCAAA ACATTTAGCT23760TTGGTTACTG AGTTTGGTAA TGGGGAAGTG AGCTATACTC TCAAAGATTA TGGGAAAAAA23820CAAGATAAAG CTTTAGATGG GGAGACAAAA ACCACTCTTC AAGGTAGCCT AAAATATGAT23880GGCGTGATGT TTGTCAATTA TTCCAATTTC AAATACACCA ACGCCTCCAA GAGTCCTGAT23940AAGGGCGTGG GCGCTACGAA TGGCGTTTCC CATTTGGAAG CAAATTTTAG CAAGGTAGCT24000GTCTTTAATT TGCCTAATTT AAATAATCTC GCTATCACTA ATTATATAAG GCGAGATTTA24060GAAGATAAAT TGTTGGCTAA AGGGTTGTCC CCACAAGAAG CTAATAAGCT CATCAAAGAC24120TTTTTGAACA GCAACAAAGA AATGGTTGGA AAAGTTTCAA ACTTCAATAA AGCTGTAGCT24180GAAGCTAAAA ACACAGGCAA TTATGATGAA GTGAAAAAAG CTCAGAAAGA TCTTGAAAAA24240TCTCTAAGGA AACGAGAGCA TTTAGAGAAA GAAGTAGCGA AAAAATTAGA GAGCAGAAAC24300GACAACAAAA ATAGAATGGA AGCAAAAGCT CAAGCTAATA GCCAAAAAGA TAAGATTTTT24360GCGTTGATCA ATAAAGAGGC TAGTAAGGAA GCAAGAGCGG CCGCTTTCGA TCCGAATCTT24420AAAGGCATTA GGAGCGAATT GTCTGATAAA CTTGAAAATA TCAACAAGAA TTTGAAAGAC24480TTTGGCAAAT CTTTTGATGA TTTCAAAAAT GGCAAAAATA ATGATTTCAG CAAGGCAGAA24540GAAACGCTAA AAGCCCTTAA AGACTCGGTG AAAGATTTAG GCATCAATCC AGAATGGATT24600TCAAAAATTG AAAACCTTAA TGCAGCTTTG AATGATTTCA AAAATGGCAA AAATAAGGAT24660TTCAGCAAGG TAACACAAGC AAAAAGCGAC CTTGAAAATT CCATTAAGGA TGTGATCATT24720AATCAAAAAA TAACGGATAA AGTTGACAAT CTCAATCAGG CTGTATCAGA GACTAAATTA24780ACAGGCGATT TCAGTAAGGT AGAGCAAGCC CTAGCCGAAC TCAAAAGCTT GTCATTGGAT24840CAAAAAAATG AAAGTTTCAA TGCTGGAAAA AATTCTGATC TACAAAAATC CGTTAAAAAT24900GGTGTGAATG GAACCCTAGT CGGTAATGGG TTGTCTAAAA CAGAAGCCGT AACGCTTACC24960AAAAATTTTT CGGACATCAG GAAAGAATTG AACGAGAAAT TATTTGGAAA TTCCAATAAC25020AATAATGATG GACTCAAAAA CAGTGCAGAG CCCATTTACG CTCAAGTTAA TAAAAAGAAA25080ACAGGACAAG TAGCTAGCCC TGAAGAGCCC ATTTACGCTC AAGTTGCTAA AAAGGTGAGT25140GCAAAAA門G ACCAACTCAA CGAAGTTACA TCAGCAATAA ATAGAAAAAT TGACCGGATT25200AACAAAATTG CATCAGCAGG TAAAGGAGTG GGCGGTTTCA GTGGAGCAGG GCGATCAGCT25260AGCCCTGAAC CCATTTACGC TACAATTGAT TTTGATGAGG CAAATCAAGC AGGCTTCCCT25320CTTAGGAGAT ACGCTGGATT TGATGATCTC AGTAAAGTAG GGCTTTCAAG GGAACAAGAA25380TTGACTCGTA GAATTGGCGA TCTCAATCAG GCGGTATCAG AAGCTAAAAC AGGTCATTTT25440GACAAACTAG AACAAAAGAT AGATGAACTC AAAGATTCTA CAAAAAAGAA TGCTTTGAAG25500CTATACATTG AAAGTGCGAA ACAAGTGCCT ACTAGTTTGC AAGCGAAATT GGACAATTAC25560GCTACTAACA GCCACACACG CATTAATAGC AATGTCCAAA ATGGAGGAAT TAATGAGAAA25620
GCGACCGGTA TGCTAACGCA AAAAAACCCT GAGTGGCTCA AGCTCGTGAA TGATAAGATA25680GTTGCGCATA ATGTGGGAAG TGCTCATTTG TCAGAGTATG ATAAAATTGG ATTCAACCAA25740AAGAATATGA AAGATTATTC TGATTCGTTC AAGTTTTCCA CCAAGTTGAA CAATGCCGTA25800AAAGATATTA AGTCTAGCTT TGTGCAATTT TTAACCAATA CATTTTCTAC AGGATCTTAC25860AGCTTGATGA AAGCAAATGT GGAACATGGA GTCAAAAATA CTACAAAAAG TGGTTTCCAG25920AAATCTTAAA GGATCGAGGA ATACTAGAAA CACCCCTTTC TAAAGAAAGG GGCTTCCTAA25980CTAAATCATT CCATTGAATA CTAACACGAA AGACTTTGTT CTTTAAAGTC TGCAAGGACA26040TTTCCTACCC CAAAAAGACT TAACCCTTTG ATAAAGATAT TTGTTCGCAG CGTTGATGTC26100TCTATGTTCT TTATACCCAC AATCAGGAAC AAATATTGCC TGTGATCAAT TTGAGCTTGT26160GGTTGATGCT CCCACAATGA TAGCAAGTTT TGCTGGTGTA TTGTGGGGGA AACTTTCACT26220AGCAATTTGC CATTATGCTG TTGTTTGTAG TCTAGCAAAC TTGTAGATAG GTAATTCTTT26280TCCAAATATA AAAACGCTTT TCTGGATCTC TCTAAAGCGC TTTATAATCA ACACAACACC26340CTTA 26344<210>121<211>11277<212>DNA<213>H.pylori<400>121GCTTAAAGGC TCTGAAGATT TTTGAGTTTG TGCCAAAACC CCTATCTTGG CTTTGAGTAA60GATTGACACT GCCACGCATA AGCAGGTTTT GCAAAAATTG CAAGAGTATC AACAATATGC120TTCGCAATTT TTAGCTTTAG TGCCTTTGAG CGCGAAAAAA TCTCAAAATT TAAACGCACT180TTTAGAATGC ATCAGTAAGC ATTTAAGCCC TAGCGCATGG CTTTTTGAAA AGGATTTGAT240GAGCGATGAA AAAATGCGCG ATATTTATAA GGAAATCATT AGGGAGAGTT TGTTTGATTT300TTTGAGCGAT GAAATCCCTT ATGAAAGCGA TGTGATGATT GATAAATTTA TAGAAGAAGA360ACGCATAGAC AAGGTGTATG CACATATTAT CGTAGAAAAA GAAAGCCAAA AAAAAATCGT420GATAGGCAAA AACGGGGTGA ATATCAAACG CATCGGGACT AGCGCGCGAT TGAAAATGCA480AGAAGTGGGC GAAAAAAAGG TTTTTTTAAA CTTGCAAGTG ATCGCTCAAA AATCATGGAG540CAAGGAAGAA AAGAGCTTGC AAAAACTGGG TTATATCTAT CAAAGGAATA GGGATTGAAA600AAAATATTAC CGGCTCTGTT AATGGGGTTT GTGGGCTTGA GTGCTGATGA GCGTTTGTTA660GAAATTATGC GCCTTTATCA AAAACAAGGC TTGGAAGTGG TGGGTCAAAA ATTGGATTCT720TATTTAGCGG ATAAATCTTT TTGGGCAGAA GAGCTTCAAA ATAAGGACAC GGATTTTGGC780TATTATCAAA ACAAGCAGTT TTTATTTGTG GCGGATAAAT CCAAGCCTAG TTTGGAGTTT840TATGAAATAG ACAACAACAT GCTTAAAAAA ATCAACAGCT CTAAAGCCCT TGTAGGCTCT900AAAAAGGGCG ATAAAACTTT AGAGGGCGAT TTGGCCACGC CTATTGGAGT GTATCGTATC960ACGCAGAAAT TAGAACGCTT GGATCAATAT TATGGCGTGT TGGCTTTTGT AACGAATTAC1020CCTAATTTGT ATGACACTTT GAAAAAACGC ACCGGGCATG GCATTTGGGT GCATGGAATG1080CCTTTAAATG GCGATCGGAA TGAATTGAAC ACCAAGGGCT GCATTGCGAT TGAAAACCCG1140CTTTTAAGCT CTTATGATAA AGTGTTAAAA GGCGAAAAAG CGTTCCTCAT CACCTATGAA1200GACAAGTTTT TCCCCAGCAC TAAAGAAGAA TTGAGCATGG TTTTAAGCTC CCTTTTTCAA1260TGGAAAGAAG CCTGGACTAG AGGCGATTTT GAACGCTACA TGCGTTTTTA TAACCCCAAT1320TTCACTCGCT ATGACGGCAT GAAATTCAAC GCTTTTAAAG AGTATAAAAA AAGGGTGTTT1380GCGAAAAACG AAAAAAAGAA TATCGCTTTT TCTTCTATTA GCGTGATCCC TTACCCCAAC1440
TCCCAAAACA AACGCTTGTT TTATGTGGTG TTTGACCAAG ATTACAAAGC CTACCAGCAA1500AACAAGCTCT CTTATAGCTC TAATTCTCAA AAAGAACTCT ATGTAGAGAT TGAAAACAAC1560CAAGTGTCTA TTATAATGGA AAAATAGGGT TTTGTTTTAA TGAGAGCGAT TTAAGCGGAT1620TTTTCTAATT TTAAACTTGA TTACAGATCT GATTACAAAT TACAGAGCTT GCTTTTTCAT1680AGCCAAATTC CACGGATTTT TGCAAGAATT TTTTAGCCCT ACTTTTTTGT TTTCTGGTGC1740CAAGCCCTTC TTTGTAAGCG ATACCAATTC TATAAAGGAT TTCCCCCATT TGTTGCTTGA1800GTAAAATAAT ATTGCTTGAA ATTTCAGAAA TATCTGCCAT AGAAAGTGCA TCAGGAACCT1860CACCGTTGCT GTGAAGGAAA TACTTAAAAT CCTCAAGTTT TTCAAATTTC TTTCCAAATT1920TTTTAGGATT TGCGCTCAAA CCTGAAAGAT CAATGAAACG CGCTCCAATA GCGATACCAA1980TCGTTTGCGC AAGGTCTAGT AATCTAAAAT TGTTATGAAA ATTTATGCCT TTATTAGAAA2040GTATGCGAAC CACATGCCTG TAAGTATTGA GCGCTTGTTT CAAATCCTTT TTCATCCCTA2100AGCCTTGCGC TTGCATGCGT CCTAAAATAA GAGCGCCCCC TAACATGCCA CTCCCATACC2160TTTTGATAGC GTTTTTTGCA TAGAGTTGCG CTTTTTTGTA ATCCACTTTC ACGCCCTTTC2220CCTCCAAATA CATTTTGGCT AAATAAACAC TGCCAAGCCC AATGCCGTAT TCTTGCGCCT2280TTCTAAAATT TTTAAAGGCT TGCTTGTATT GGCTTTTATT AAAATTGTCT AAACCATTTT2340CAAAATAATA TGTCTTATAT TTTTGCGTGT ATTCATCCCA AGTAGGATTT ATGCTAATAT2400ATCCATCAGC TATACAAAGT GAAAACGGCA ATAATAAACC TAATAAAAGC AATAAAGGCT2460TTAAAAAAGA AAAAGTGGTG CGATAAGAAT CTTTAAACAT GTCGGATCCT TTTACAATTT2520GAGCCATTCT TTATAGCATA ATGGCAACAT GGAGATGGTT TTATATTTTT TGCTATAATG2580ACATGAATTT GTTTCATAGT AACAAATTGA GAATATACCA TCTATGGAGG TAACACTATG2640GCTGACACAA TCAATACAAC TGAAGCAACC CATGAAGCAA AAAAGCCAAA CGCTTTTGTG2700AATTTTTTCA AAAACAATTT GGCTGATAAG CGTTATGATT CATTAGGTCT CATTGGAGCA2760GGGGTTTTAT GTTGTGTCTT GAGCGGTGCT ATGGGGATTG TCGGGATAAT CTTTGTCGCA2820ATAGGAATCT TTTTGTCTTT TTCTAATATC AATCTAGTGA AATTAGTTGA AAAATTGTCC2880AAAAAGCAAT CTAAAGCGGC AACAACTGTC AATAACGAAA CCCAAAAATC TCAAGCAACA2940AGCGTTACCA ACGAACCAAC TGAAGCTAAA GAGACTAAAG ATTGAGGCAA AACAACGATT3000TTGACTAAAG AAAGAATAAG AGAAATTTTC AAAAGTTTAG GTGTTCTAGT GGTTTATTAC3060TCACTGCATG ACTCACACAA GTGGCCTATA AATTCTTTTT TTAGATTTAA GAATGGTTAT3120TTGCAATATG AGTCTAGCTA CGAGTTGATG GGGTTGATCA AATCCATTGG CAAGGCTTTA3180AAAATGGAAA CCAACTGAAA AAAAGGAAAC TAATGTTTAG AAAACTAGCA ACCGCTGTAT3240CGCTCATAGG CTTACTAACC TCTAACACTC TTTATGCTAA AGAAATAAGT GAAGCCGATA3300AGGTCATTAA GGCCACTAAA GAAACTAAAG AAACCAAGAA AGAAGCTAAA CGACTCAAAA3360AAGAAGCCAG ACAGCGCCAA CAGATCCCTG ATCATAAGAA ACCTCAATAT GTTTCTGTTG3420ATGACACAAA AACTCAAGCG CTTTTTGATA TATACGACAC CTTGAATGTG AATGACAAAA3480GCTTTGGGGA TTGGTTTGGT AATAGCGCTT TGAAAGACAA AACCTATCTC TACGCTATGG3540ATCTATTGGA TTACAACAAC TATCTATCCA TAGAAAACCC CATTATCAAA ACAAGAGCGA3600TGGGGACTTA TGCGGATCTC ATCATCATCA CAGGCTCACT AGAACAAGTC AATGGGTATT3660ACAACATTCT AAAAGCGCTC AACAAACGCA ACGCTAAGTT TGTCTTAAAA ATCAATGAAA3720ACCTGCCTTA CGCCCAAGCG ACTTTTTTGA GAGTGCCAAA AAGAAGCGAT CCCAATGCCC3780ACACGCTTGA TAAGGGAGCG TCAATTGATG AGAATAAGCT TTTTGAACAA CAAAAACGCG3840CGTATTTCAA CTACGCCAAC GATGTGATCT GCAGACCCAA TGATGAAGTG TGTTCGCCCC3900TAAGAGATGA AATGGTAGCT ATGCCCACTA GCGATAGCGC TACTCAAAAA CCTAATATCA3960TGCTCCTTAT AGCTTGTATA GACTAAAAGA GACAGATAAC GCCAATGAGG CTCAACCATC4020ACCTTATGCC ACTCAAACCG CTCCTGAAAA CAGCAGAGAG AAGCTCATAG AAGAGCTAAT4080CGCTAACTCC CAACTCATAG CCAATGAAGA AGAGAGGGAA AAGAAACTCT TAGCAGAAAA4140AGAAAAACAA GAGGCTGAAT TGGCTAAATA CAAGCTCAAA GACCTAGAAA ATCAAAAGAA4200
ACTAAAAGCT TTAGAAGCAG AGTTGAAAAA GAAAAATGCT AAGAAACCTA GAGTAGTGGA4260AGTGCCTATT TCTCCTAAGA CAAGTAATTC TTTTGAAACA ATGAGGGTTG TCAAAGAAAA4320AGAAAACTAT AATGGATTAT TAGTGGATAA AGAAACCACG ATCAAACGAA GCTATCAAGG4380GACTTTGATT AGTGAAAATT CTTACAGCAA AAAAACACCT CTCAACCCTA ATGACTTGAG4440GAGCTTAGAA GAAGAAATCA AAAGCTACTA TATCAAGTCT AATGGTTTGT GTTATGCTAA4500TGGCATCAGT CTTTATGTCA AAATCAAAAA CGACCCCTAT AAAGAGGGAA TGCTGTGTGG4560CTATGAGAGT ATTCAAAATC TGCTCTCACC TCTAAAGGAC AAGCTCAAGT ACGACAAGCA4620AAAATTACAA AAAGCGTTAC TGAAAGATTC AAAGTAAGTT AAAGTTTTGT TTGAGAAATG4680GATTGGTCTC TTTTTACTTC TTAATTCCTT AGCCTATCCA TGCCAAAAAG TAACCATTAG4740TTTCAAGCAG TATGAGAATC TTATCCATAT TCACCAAAAA GGTTGCGACA ATGAAGTGGT4800GTGCAGAACG CTCATCTCTA TCGCTTTATT GGAAAGTTCT TTAGGGTTGA ACAACAAGCG4860CGAAATTTCC CTAAAAGACA CTTCCTATTC CATGTTTCAC ATCACTTTGA ACACCGCTAA4920AAAATTCTAC CCCACCTATT CTAAAACGCT TCTCAAGTTC AAATTGCTCA ATGATGTGGA4980TTTTGCGATT TCATTGGCTA AAAGAATTCT GAAAGACGAT TTTGATTACT ACTAACAAAA5040ATACCTTAAT AAAAGCGTGT ATCAATTAGT AGAAATGGCA GTAGGCGCTT ACAATGGGGG5100AATGAAACAC AACCCTAATG GCGCTTATGT GAAAAAGTTC CGCTGTGTCT ATTCTCAGGT5160GCGCTACAAC GAGTAGAGCA TACTAATTTT ACAAACAATC TTGGTGATAC ACTTCTACTA5220TCTTATGAAT TTATAATAAT ATCATTTGCA TCATTATCTT TTAATTTTTC TAAAATATAT5280TTCGCTTCTT TTTGTTAAGA TATTTTGTCT TTTTATTTTT TGGAATAGGC TTTTTGCTTT5340CCTTATAAGG ACATATAGCG TAAGAATTTT TCCAATTCAT ACGATTTTGG CATAGGTGCT5400GTAAAAATGG ATTGATGAAT TTTAACCTAG CGCATGCAAA CAATCCACTA GAGCGATGGT5460TGGCAAATCA AAGAACACAC AAATCACAGT TCGCTTGAAC CCACAGGCAC TAAAGATTTA5520TCATAGCTCA ATTCGCCTCT AGTTTTAGCG GCTTGCAAAT CATCATAGAA TGTTGCTTGA5580TTAGGCACTT TCCCTAATTT GTATTTCTTG CTCAAGCTTG GACTTACCTT AATAAGCTCA5640TCAGTGAAGA ATGGATCATC GTAGTAAAGC GCCTTGTGGC ACTTGATGGG TTTGAGCGTG5700TTTTCTAGAA TGATAAGCTC ATCGCCCATA GTCATCAATT CATCAGGGGT CATCAAAAAC5760CGCTCCTTGT TGCTGATAGA AGTATTGGTC TTACCGGTAT TATCATCAAT GCTTCGGCTC5820ACATCTTGTC TGGTGTATTT CCCTAACACC TTAGAAAGTT TTTCAAAATG TTCATAGTAG5880TTATCGTTGT TAATCCCATA ATACATATTC AAAGAAAGGT TGTCTAAAAT AGTCTTAGCG5940CCATTCCTAC CATAACCAAG TGGGGGGTCA TTCTCTAGTT GCGCCTTACT TTGAAACACA6000AACGCAGGGC GCATGTTGTA TTCTGCCATA ATCCCTACCG CTTTAACAAA GGTCTCTAAA6060TAGCCACAAA GCGTAAATTC GTCCATGAGC ATTAAACAAC TTCTTTTGCA TTGTGGATCA6120TGGATTGGCA GAATAAGGTT GCTATAAATC ATCACATTGA AAAACAGCTC TAATATCGGC6180CCAACAATAG TGCTTTCTTT AGGATTAGCG ATCACGCCAA TACTCACTTC ATCAATCCTT6240AAACGCCTGA AATCAAAATC ATTAGCACTC GTGAAATTTC TGATCATGGC GTTATTGTAA6300GGTGCAAACG CTGAAGTATA GACCCCTTGA ACAGAGCTGT AAGTTTCTTT AGCGCCGCCC6360ATTGTCTTGA AATTATTCCA CATGTTTCTA GTGGCAGGAC TAAGCACTCT TATATTATCG6420CCACTCTTAT CTTCTTCACC TCCAAAAAAT TCCATCAGAG ACACGACTTT TTCCATGTTT6480GTGTCTTCAT CAATCAAGTT GATACCGCTT GCCATAGAAC CTATGAAAAA CATCGTGGGG6540GTTTCAGGCA TGATAATTTT TTTTCTTTTG ACAAACTCAA GCCCCTTTTT AGTCCACATG6600AGATCCCTAT AAATATTGCA ATTGATGACA AAAAGGTTTC GTGCTTGATT GCTAAAAAAA6660GGATCTTTTT CATTAGGTCT TTCAGGGAAC ACGAGCTTTG CCAATCCAAA GATTTGAGTG6720GAAAAATCCC CCCCACTAGC CACCATCCCA TGACCTTTTA GGCGTGTGTC AATTTGAGAG6780AGTATGTCTT CAGTTAAAAC CACATCATTA CCAAAATCCA CATAAGCGAA AGGATTAAAT6840CGGTGTGTTT TTAAGGAGAA AGGTTCATAG ATGAACACTT TTTGGTTGAA GCGTTTTTCT6900CTGATTTTTC CGCAAGTTTC CATAGTGTCA GCCTTAGGGT CAAACACAAC GATATTTTGA6960
GGATAATTGA TCATATTAGG CATGATGAAC CCCACCCCCT TACCGCTTCT AGTGGGAGCA7020ATCAAGCCAA TGAACGCCTG CCCTGCATAA GCGATAAAAT CCCCTAAACC ACGCCTGCCT7080ACAATAACTT CTCGTTTGTC AAAGGCGCGT TTTTTATTAT TGGGTGTGAT GAGTTTAGCT7140TTGATCATTT TCTCTTCGCT CTCCCAACTC GCGCTACCAA AAAGATCATC CACTTTTTTA7200TTCGCTCCTA TGTCTCTAGT CCGAGTTAAG TATTTTAAGA ACCACACAAA AAAGGTAATG7260AATATATAAG AGCATAAAAT AGAACTATAT ACGACTAAGG ATATATTGAA CCCATAAACT7320TTGAGTGAAC TAAAACAAAG CGCCTTAAAA TAGTATGTAG GAAAATCTTG TATCGCATAG7380ATCCATATAT CAAAAAGATC ATCATCACTC TCAGGCAATT TATTATCATC ATAAAAATTA7440CCTAAGAGAA TCAAGAAAAA TAGCCCTGAT GTGATAAAAG AATAGGATAG TAATAGTGTC7500GCTTGTAACC AAAAGCCAAA GGTTTTAACT CTTTCTTTCA GCCCCTCTAT AATGATTTCT7560TTAAAGTTTT TTTTCTTTTG AGGTTGGTTA GCTTTATCTT TAAAAGCTTT TTTTTGAGCT7620TTTATGAATT TGTAGAGAAA AAATAACGCT ATTACCCCTA TGAAACTAAA AATAACAGCT7680AACTTATAAT CCTCTATGAA ATATAAGGTG TTATACAACA AGTCTTCCAT TGTGCCTACT7740ACCTATGTTT GATATAAAAT TCATCACACT GTTTGTGGTG GTTGATATGG ACAATCATAT7800CAATTAAATC TTTAAAGCCC TCAATGAGGC TTTCAAACTT GATATTCCTT GCTGCGTTAT7860TAGATGAACT CATGTTGGCT AAACGGATAA ACGCTTCTTC ACTGCTCCCT GCATGTAGAG7920TGGTTAGCGT GCCTTTATGA CCGCTACAAA GCACATTATA AAAATCATAT GCCTCACTGC7980TTCTGAGTTC TCCTAAAATG ATTCTATCAG GTCGCATTCT CAAACATGAC TTTAAGCAAT8040CAGCAGAGGT GATATTCCCA CCAAAGAAAA GCTGTGTGTA GTTCTTGTGG TGTTTGAATA8100CAATCTCTTC GGTGTCTTCA ATGGATATGA TCCTTTCTTC TTTGGGGATA AACTCCATGA8160TGCTTTTGAT ATAAGTCGTT TTACCGCTTC CTGTGCCACC ACAAACAATC ACATTTTTAC8220CAATAGCAAT ACCATCTTTA ATCGCACTGA TCGCTTGTTC TTTGTTATCT AGTAGATTAT8280AAAAACCCTG TTCTTCAAAG AAGCTATGAG GATAGGTTGT TTTGCTAGGT ATCCTTATAG8340ATATGGAAAT GGTTTCATCA TTAACTGTAA CAGGGGAAAG GACAATCTGC ACCCTTTCAC8400CATTCGCTAA ATTGCTGCTC AAAATAGGAT TTTCATAGTT GTCTATTGTT TTTTTCTTAA8460AACTTGCACA ACACCGAGCA AAATGCATTA AACGAGATAG ACTAAAGGCT TTCTTGTCTC8520TCACATCAAA TGGTTGCCAT TCGCCATTAT TTTTTAAAAC CCATACAACC TTGTTCCCAT8580TGTAACAAAT CTCAGTGATA TTTTCCATTT TTAAAAAATC ACCAAAAAGT TCTTCAGTAG8640CATGCCTTAG AGGATTTAAT GCCGCTTCTT TTAAAGCTCT TTCTACTTCT AGAAATTTTT8700TATCTTCTGC ACTCAATCTG TCTTCAGTCA TGTTCTCATT CCACATTTAA TTTTAATTGG8760GTTATCAATT TAGCTCTTTC TAAAGAAGTG AGATAAGTCT TATTTTTGCT CAAAATAATA8820TATTTAGTCC CTATATCTTG GACAATCTCA TTGAGCTTCC TTCCAAAATC ATCTATCCCT8880AGAATCTGAA ACGCCACGCC CATCATATTG CCATCTATGA ATGGCGGTAA ATACTCTCTT8940GGCAGATAAG AAGCCACTAG GTCTGAAATT CTTGCTACTT CATCACACAT AAAATTATTG9000AAGTTGTCAG AAATTTCTAC AACAAACTCC CCAAGCGTTA GTTCATTTTT GATCGTGCTT9060TCTGCAAAAG TCTTTTCTTG TTTGCAACGA TTGTAACTGG CTAACACAAG GTTGTTCAAA9120TAGTTGTAAT AAGCATCAAA ACTAATAAAA TTCTTTTGCA TATTGATTCT TAACTCTTGA9180ACTCTTCTCA AAAAATCTTG CGGATCGTTG CTATCTTTTA AAGAAGTGGC GTAATTTTGA9240ATAAACTTAT CCCTTATTTC ATTTGCATTT CCCATAAGAG ATCTGTTGCT ATCTAAGATT9300TTTTGTCTTT CTGTTTCATT GAAACCGAGT CCCATTGATA TTTCCTTTCA TTGATAGTGC9360TTAGATTTTC TTCCGCCACT ACATCCTCCA CAATACCTGT CGTTGGTTTT GTCTTTTATC9420TTGTGAAGAA CCAAGTTTGG TAACACAGAT ATATTTTATC CTTTCTTTTG AATTAATTGC9480CACCTTTGGG GCTTGTGGTG ATTTCTTCAT GCTCTCTAGA CAAAGATTTA GTGCTTTGCT9540TGATAATTTC ATCTACCACA GATTTGTTGG TGATTTTAAC ATCATACACG CCACTATAAT9600CAATATCGTC CATTGTGAGA ATCTTGATAC TATCGCCCTC ATTATTGTAA ATACTTGACG9660GGATATTCAT CAGTTGCCCT AGAATTTGAT TAGACATCTG AGCTGAACTT TGCATACTAC9720
CATTGATAGC TTGACCCAAA GCGTAATTAA ATTCAGGTAT CCTTTCACTT CTACCTTTGC9780CAAGGCCTAT GAGTTATCTA GAGCTATGAT AGGCGCAGTT TGCAAGAAGC TATTAACCAC9840GCTTGCTATC ACAGCAAAGC TCTATGCGCT TCATAAAGTG GTTATTCACA TAGCCATCTA9900CCCCTGCTTC ACCCAACATG CCTGCTGCTT GAGCGTTTGC TAGAGGTATT ATCACACCAT9960CAGGCGTAAT GGCTTTAGTA AAGACTATCA TTAAGCGTGT CATAATGGGT GTGCCACCCC10020TTCACGCTTT GATAATTCCC ATACACCTTA GTGCCTTTGT CTAGTAAGAT CATAGTGCCG10080TTCATGTTCC ATACATCTTT GGCTACAACC CCACTCACTA TACCTGTGAG AGTGGCGTCT10140ACTTTAGAAG TCAGAGTGAT TTCAATAGGG GTGTATTGCG CTAAAACAAA TGTGGGATCA10200CTCTTGCCTA TAAAGGCCTG TTTGACAGGG CTTGTTTCAT CTTGTGTTTC TGTTTTGCTA10260TTTGTCATCA ATGGGGTTAC CATTTTCATC TACACATTCC CCACTACTTT CTTTTTTGTT10320AGTAAGAGCG AATTCAGTAA TATTTTTGGC AGTCTCTGCG ACATCTTTGT CTATCTTATT10380GCTTTCTACT CTAGCTTCTG CTGTGGATTT TTTACTTTTT TCTTTATCGT CATCTTTTTT10440ATTGCCACCT AAAGCCTTAG CTAATTTAGC TTCTAAATTC TTATCCTTAA TGGTTTTTTC10500TGTTTCGTAT TGCTTGGTAA TGTCAGGTTC TATTGAAGCA TAAATAGGAT TATCGCTAGC10560TATTTTGTCC GCATCAACAT TAGTGGCGTT AATAGTGGCA ATATCACCGC CATTTTTGAA10620ATCCATTGGC AACAATGGAT AACCTTTAGC CGCCATGTTA TCAAAGGTTT TGCGGTTTCT10680TAGATCGCTA TAAATCAGAT CCACTTCATC AGCTTGTCGT TTAATTCCTA GAATTAGCGC10740CCTTTCACTA TCGCTCAAGC CCTCTAAACA TTGCTCTATG GCTTCTTGAT CAGTAGGGTC10800ATCTAAGTTA TCCAAGCACT CGCTTGCTTG ATGCAATCTT TCTGTTTTAC TCAAATGATT10860TTGTTTGCCT TGTGTCCTTT TATTTTGGAT TTCTTGGATC AAATCGCTAT AAAGGTTTTG10920GCATTTCCTT TTTTCTTCAT CGGTCCTAGC CGTTTTCAAA CAATCCAAGA CAGCCTTTTC10980TCTAGCTTCT TGCAGGTATT TGAGCTTCTC TTCATCGCTC AAACCATCCA AACACTTCAT11040AATGGCCGCT CTGTCGTTAG GATCGGCGTT TTTCAAGCAA TCTTTGATCG CTTTATCTTT11100TCGTTGGAGT TCTTTCGCTA AAAATTTTCT CGCTTCAGGG GTGAGTAATT TCTCGCACTC11160TTGTTTCTCT TTTTCATTCC TAGCTCTTGA AACGCAGTCC AAATAGGCCT TAACGCTCTT11220GTTGGCTCTA TGCTATAAGA GTTTTCTCGC TTCAGGGGTG AGTACTTCTC GCAAGCT 11277<210>122<211>24404<212>DNA<213>H.pylori<400>122GGTTTTTTCG CTTAGGGGTG AGTAATTTCT CGCATTCTTG TTTCTCAGCT TCAGTTTTGG60CTTGAGATAC GCAGTCTTTA TAAGCTTTAA CGCTCTCTTT AGCTAAAATA TCGCTTTGTA120AGTCTTTAGG GAGATCTTTC AAACACTTTT TTCGTTCTTC ATCAGTTTTA GCGTTTTTCA180AACAATCTAG AACCTGTTGT TCTAACTTTT TTTTCGCTTC AGGCGTGAGT AATTTCTCGC240ATTCTTTTTT CTCTTTTTCA TTCCTAGCTT TTGATACGCA ATCCTTGTAA GCCTTGACGC300TCATATCAGC TAGTAGTTCT TTTTGCAAGT CTTGGGGAAT ATTTTTCAAG CACTCGTTTC360GTTCTTCATC GGTTTTAGCG TTTTTCAAAC AATCTAGTGC TTGCTGTTTC AATCTTTCTA420TAGCTTCTTT AGACAAGCCT TTCAAGCATT CGTTTTTCTC AGCTTCAGTT TTGGCGTTTT480TGATACAATC CTTATACCCT TGAAGTTCTT TTTGAAGCCC TAATTCCTTA CGGAATTTCT540CTCTAATCTC AGGGTCATTT ATGAGTTTTA GGCACTCGTT TCGTTCTTCA TCGGTTTTAG600CGTTTTTTAA ACAATCTAGC GCCACTTGAA CTTTTTGTTG GTTCAGTAAG CTTTTTTTCA660GGTTTTGATC TTTGATTAAA TCTAAACACT TGATTCTTTC TTCTTCAGTT TTGGCATTTT720
TGATGCAGTC ATTATAAGCC TCTAAAGTCT TTTTCATTTG ATCTTGAAGT TTTTTGTCTT780TGATAAGCTT CAAACATTCT TCATAGTTGC CACCATTACT AATACATTCA TAAAAGGCTC840TCAACGGGTT TTTGTCCTCA ATTTCTGCAA TATTCAAATA GTTGTATAAG GTTCTATTGG900GATCGTCATT GAAGAAAAGA TTCTTATCGA TCATATTGCC TTTTTCATTC CGTTCTTTCA960GCAATCGGTT ATACTCTTGC CTTATTTGGA TTTCATCATT GACATAAAGA TTCCTGTCTT1020TGCTAAAACG AGAGCTTTTA TCTTCCAAAG GTATGAAGTA GTGAAAAATG CTTCTAGAAA1080ATAAAATAAT CACGATAAGA ACAGCGACTA CAATGCCACC AATAATGTAT TTCTTTTTGC1140TTCCTTTGAT AATCTCTTGA TCGTTAGAAT CGTTAGTTAT TTCTTCTGAC TCGTATTCAT1200CAAAATTGGT TTTAGTTTCT GTGGGGTTGT CAAGATGATG ATCTGAGCTT TCTTTGGATT1260CTTTTGAAGA ATCTTCAAAG TGATTGGCTT CTGTTGCTTC TTCATTGGAT AAATCTTGGG1320GTGAATGTTG TTGGGTTTTT TTAGAAGTTT CATCATCAAT TAAGTCCATT TGAGTTTCTT1380CTTGATACCC ATTATTTTCT TGATCGTCTT CTTCGTTCGG TTCTTGGATT TCTTCATCTA1440AATTCTTTTG CTGAGTGAAA GGTCTTTTAT CATCAACTAA TTTTCTAGCT TTTTTGAATA1500ACTTGTCTGC TAGACTGCCA TTGCTAGATT CTGAAGTTTC ATTATCTCCA GAATCCATTT1560GAGTTTGGGT TTCTTCTGAC TCGTATTCAT CAAAATTGGT TTTAGTTTCT GTGGGGTTGT1620CAAGATGATG ATCTGAGCTT TCTTTGGATT CTTTTGAAGA ATCTTCAAAG TGATTGGCTT1680CTGTTGCTTC TTCATTGGAT AAATCTTGGG GTGAATATTG TTGGGTTTTT TTAGAAGTTT1740CAAGTTTATC GTTTTCTTCA TTCATGTCTT AACGCCTTTT TATTTATCTC TGACAAGAGG1800GAGCTTTTTA ATCACACGCT CCAATTCACC ATAGTTTTTG ATATTGTAAT TTTTTGTCAA1860TGGATTTTTC CCATAGCCTT TATTTATTAC TGTTACAAGG GCCTTGTCTT TAATGAGCTT1920AAACTTTTCT GCAATTTCAT TAACTCTATA CCATCTCAAT CCTGAATTGG TCATGTTAGG1980ATCAATAGCA GCATCAGTCA TGCTTAATTT CCCATCAGGC TGAACCACAA AAATAGCAGG2040TTGGAGAGTG ATGTTTTTGA AACCAAAATA AGTGAATGTG CCATCATCAA AAATTTCAGA2100GGGCATAATA TGCTTAGAGC GTTTCTCAGG CGCTTGGTAG TAATTGTAGT TTCTAGGCAC2160AGGGTTTCTT TTTAATGCAT TATGCACATA TTGAGTCTCT AGTGCCTTTG CTTGATCTAA2220AATAATTTTT TGTTTTTCTT CTCTTATTTT TTCTTTGTTG ATAATCTGTT CATTGCTCGC2280CATCATTACT CTATTGATGT AGGCTGTGGT ATTAAGATTT TCTTGCTTGA TCAATTCTCT2340CTGCCTCTTT GCTTCTTCTC TCTTTTTTAA CTCCTCTTCA ATAACGCTAG AGACTTCGTG2400TCTTTGTGGG TATTCTAATT TGACTGTTAG ATACGCCGAA GCAAAATTGT CTTTTTGAGC2460TATTCTCAAA ATGAATTGAT ACAAGGCTTT ATTAGTCCGC ACAACAAGAT TAGTTCTCCA2520AGCGCTATCG CTAGGAGATA ATTCTATGGA GTTATCCTCA GGGCTTTTTT GAGACTTATC2580TGTCTTAATA TTGATTTTAT CCTTGGCTCT TTGTTTCACT GCCTCTTCAG CTTGTTTTTT2640GTTGAGTTCT TCAATTTGTT TGAGCGCATT AGCTTGAGCC TGTTCTTGCA TGTCCTCTAG2700TCGTTCCATT TGATCTAATT CATTTTCTCG TTGTTGCTTG ATAAATTCAC TAAGATTTTT2760ATTATTGCTC AAATTTTGTG GGTTACTCAT AGCGTTAGTG AGATTTTCTA AATTGGCTCT2820ATTTTTTGCA CGCTCTTCCT TTCTTTTTTC TCTTTTATCT TTTTGCGCTT TTTGCGCCTG2880TTCTTTAGCT TCTTTTTCTT TTTCTAGAGC TTTTTTTTGT TCTTCTAATT CTTTAGGGTC2940AGGCGCATCT ACGATAAGTT TTTTTGTTTT TAAAAATTCT TGATAATCTC TTGTCATTAG3000GGCAAAATTC ACTGCTTCTT TTTCAAACAT GAGATTACTT TTTACCGATT TAGGTTGAAT3060GAATATATGA TTAGAATTAG GCACAATACT CCAACCTTTA TTGAAACCTG TTGTGATGTA3120AGAAATAGTT TCATCTTTTT CAAGTTGGAT CACGGTAACA TTGTCTAATG AAGTCCAAAT3180CGTAATAGGT TTTTCATCTC CCAAATAAGC AATCTTTTTA TTCACCACTT TCACCCTACC3240ACGATTAAAA TTTTTAATGT CAGGTGCTGC TGCTTCTATT ACGCTAGATA AAAATAAATA3300ACCAAGACAG AAACAGCCAA CAATTTTTTT AAAAAATGCC TGTTCCATCA ACAATTCCTC3360TTAAAAAATA TTTGTAATTG TAACACAAGA ATAAAGTGGC TTATCCTTTA AACATGGATC3420CACCAACTCT TCCCACCATT AAGGCATGTC TTTCTCTATC CATTCTGCTA CCATCCATAG3480
ATACAGAGCT GAAAATGGTA TTAACGATCG CATTGACACT CTCAATAATC GTCTCTATGA3540AACCTTTTAA AATAAATAAA GTGATCAAAC TCGCAATAGT TGAATCGATA CCCAATCCCC3600CTTGGTTGAT GTTTTGTATA GCAACAGAGG GCGTTATGGT CAAATTAAAT GCTAATAATG3660CCAAAACACC AAAGAGCAAA ATAGGACTAG CTAGAACCAA GATAAACAAA TTATTAATAA3720ACCATATCAA TATGTTTTTC ATAGAATCTT TGAACCAATC TAGCACGATC AAACACACCG3780CAATAGGGAA CACAATACCG CTAAAAGCCC CAGCTATCAA AGGTTTCACA ACAACAGTGC3840TTATTCTCAT AATGATAGTT ACTTGCATAA AAATAGCGAA TAAAGAAATA CCTGCCACTT3900GCAAGCCTTG TAACAAACTA GGAGCGTTGT TGCCAACATT AGAAAAAACA GCTCTTACCG3960CAGGTAAAAT TGAATTAACA GTGTAATTCA GCACTAAATC TAAAGCCCAA AAAAGAACTT4020GATTCAGCCC AAAAGCGCCT GCTAAACCAA AATAAATGGA ATAAGAAATT TTATAAAGAT4080AACCAAAACC TGCATAGGCG ATAAAAGAGA CCATCGCCAT TTTGAATAAT GTCCCCCCAA4140CAAATCGCAT GTATTCAAAA CCTATGTTAA TCTGAATGCC ATTTGAAATA TCTCTCAATT4200TCATAAAAGA AATAAAAACA AAAATCCCAA AAACAATATA AATCAGGTAA GAAACAAAAT4260CAAGATATGA CCCTTGCCCT ATGATAATAT CAGGTAGATT GACTACACCA TATGCAGGAA4320GAACAGCAGC GTCCGCTCCT TTGACAACAG ATTCTAGTGC GGCAAAAGCA AGCATGGCTG4380TAGTGTTGTT TTGCAGTTTA TTTGCAGTGA ATTCTTTACC AATCAGCCAA ATTAAACCAT4440CAGCTGTAAT ATTCGTAGAA GCTTGAGCGG TTATTGAGCT AATTATTTGC AAAAGAATAT4500TGCCTTTCAC ATTGTCCTCA ACACCGCCTT TGGTAAAATT CTGAATCTTG TTGCTCAAAT4560TGTCTAGCAC GCTATCCATG GATACATCTA TGTTTTTATT TATCAATGCG TTCCAACCAA4620AATTACAGCC ACTTGATGGC GTTAGAGCAA AACCTTGTAT AGCCGTAGCA ACACTTGCCC4680ATGCCGATCC CCATAAAGGA CCTAAAGTAT TCAAATATTT CACCAGATTG CTAGCGGCTA4740AACTTGTGGC TAATGGTGTG CTAAGCGCTT CGATCACACC CTGCCCTGAA CTCTTTCCAC4800TCCCTGATGC ACCAATAGAT GTATAGCCTG TCAAAATATC TGCAAAACTT TTGTTGGTTA4860AAACATAACT CGGTATCAGC ATGCAATAAA CTGTTTGGTA AATTTTGAGA TTTGAAACAA4920TGGGCAAATC AATAGCTTTC AACCAATTAG GAACAATGAG AAAAAAACTC ATGATCGTTA4980TTAAAAAAGT TCTTTTAATA TTAAACATGT TCTTATTTAT TTAATGCCTT ATTTTTTGAT5040TCCACTTCTT TAATCTTTCT CAAAATTTCG CTTACCGTCT GTTCGTTTTG TAAATCTGTG5100ATTTGAATGT CAAACACTTT GAAGCCAAAA GGATTGATGA TAAGATTTTC TTGAGAAGAG5160TTACCTCTGG CAAAATCATA ATAAATAGTT ACTTGTTTTT TAGTAATATA TTCATAATTT5220TCCATTGTAT CGGGTGTGAT TTTGATGGTA ATGAAAAATG TTAATCTCGT TAAGGGACTA5280TTTTTGACTT TTTCTCTTTG TATGTTGGAG CTAATGATAG CTTCTGCTCG GACTTTATCT5340ACAAATTGTC TGATATTTTC ATTGAACATT CTCATTGCTT GGGTTTGGAA ACTCACATCG5400CAATACTGCA TTAGTTGATC CTTGCGATCC CTCAAAGAAT TTTTGCTATA ACCAAACAGC5460AATGAAACAA ATTTTGAAGT TGCACTATCC ACAACAGCTT CAGAATTGAC AATTTGCCTA5520GCATCGGATC GTTTGACAAT TTTAAATTCT CCTGTGTATC GATCAATGCC ATAAACAAAT5580ATATCTGTTT TCTTCAAAGG CATCATCATC ACAATACTTG CTACAGCGGC GATATTGAGT5640GCCATAGAGA GATAAAAAGC CCTTTTGAAA GTCCTATTGG CTTTATTGAG TTTTGCCAAT5700CTATCAAGGG CTATCACACC CCCAACTAAA TTTTCATCAA TCAAGACTTC CTCGTTTTTT5760TTCCCTAACA TGCGACAGCT TTATGGTTTA GTTATTGGTT TGTTGGTTGT AAAATTTGAG5820AAATTTTTTC ATTAAGAACC CCTTAAAATA CCTTAAATTG TAGTATAGCA AAATTTCTGT5880AAAAACTATG TATTTAATTT AAGGTAAGAG CAATGAGTAA GCAGTCGTAA CCCAGTAATG5940AAATTTTAGA AGCGCCTAAT TCAAAGAGGT AAGACAATCA CAACTCAGAG AGAGAATGGT6000ATAATAAGAA AACAATTTCT CAATTTAATT CTTGAAAGGA GTTTCATGAA CGATACAACA6060GAGCATCATG GATCCAATTC GCTAAACGCC CCATCACCTA GCAACTCACA GAGCAATGAT6120CTTTTAAATT TGCTAGACTC GTTATATCCC AAAGGGAGTT TAGGGGAGCA AAGATTTCAC6180GAAGCTCTAA AGAATCAAGA AGAGTTGAAA AATATCCTAA TAGAAATAGA AAAGCTACCG6240
CAAGAAAAAA GGTATGAACT TCTGATGCAG ATAGGGCAAG CCAAACAGAG AATAATGGAA6300GCATACGCTC ATTCATTCTT GGGATATATA GGGGGACTAG AGCATCTGTT AGGATTATGT6360ATGGGTGGGA TATTTGTTCT GTTTGCAATC TATTTTGTAT TTTTAAGAAC TAGCAAAAAC6420ACGGATTTGG TGGAAAGTCT AAAAACAAAA CTAAAACTTC AGTATTTTTA CTATGCCTTT6480GGTGCAGGTG CGGTTTTGTT TTTTGGATTA GAAACAATTA GATCTATTTA TGAACTATAT6540ATATTAGGAA TTGGTAGCAC TAACGATAAG GTGCTCTTTG TTTTGAAAAA CATTTGCTTC6600ATAGGTATGG GCTATTTGAT TTATAAAGTT ATTAAGGTTA TTGGTATAAA AAATTTTATC6660AATGGTCTTT TCACTTCAAA GAAACAAGAA TAATAAGGAG AAACAACAAT GAGAATGAGA6720GCAAGTGTTT TAATCGGTGC GACAATTCTG TGCTTAATTT TAAGCGCATG CAGTAATTAT6780GCGAAAAAAG TGGTGAAACA AAAGAACCAT GTTTATACGC CTGTGTATAA TGAACTGATA6840GAGAAGTATA GTGAGATACC CTTAAATGAC AAACTCAAAG ACACACCATT CATGGTGCAA6900GTGAAGTTGC CAAATTACAA GGACTATTTG TTGGATAATA AACAAGTTGT ATTAACTTTC6960AAACTCGTTC ATCATTCTAA AAAGATTACG CTCATAGGCG ATGCCAATAA GATACTTCAA7020TACAAGAATT ACTTCCAAGC TAATGGAGCA AGATCTGATA TTGATTTTTA CTTGCAGCCT7080ACTTTGAATC AAAAGGTGTG GTGATGATAG CGAGTAACTA CAATGATAAT CCCAACAACA7140AAGAACAACC ACAGACCTTT GATGTGTTGC AAGGAAGTCA GCCAATGCTA GGAGCTAACA7200CAAAAAACTT GCATGGCTAT GATGTGAGTG GAGCAAACAA CAAGCAAGTG ATCAATGAAG7260TGGCAAGAGA AAAAGCTCAG CTAGAAAAAA TCAATCAGTA TTACAAAACG CTCTTACAAG7320ACAAGGAACA AGAATATACC ACTAGGAAAA ATAACCAACG AGAAATTTTA GAAACATTGA7380GTAATCGTGC AGGTTATCAA ATGAGGCAGA ATGTGATTAG TTCTGAGATT TTTAAGAATG7440GCAACTTGAA CATGCAGGCC AAAGAAGAAG AAGTTAGGGA GAAGCTGCAA GAAGAAAGAG7500AGAATGAATA CTTGCGCAAT CAAATCAGAA GTTTGCTCAG TGGTAAGTGA TTGGAAGAAA7560AGGAGAGAGT AGATTTTTTT AAGGGTAGAG AGCAACGCCA ATAGGCGTTA GCCTTACTTG7620ATAGGTGAGG CGATCAAGTA GGTAATCTTT TTAAAGCACT CATTATTTTT TAATTCTATC7680TCTGCCTAAG ACAAATATTT TTCAAACTGA AGTTTGTTAG TTACACCGAG ATTTTTTGTT7740CGTTTTCCAC ATCAAGCATT TTGCACTCCT TTTTCTTTCA TGTATTTTTC AAGATCAAAT7800TTCATTAGCA AATCCCTAAC ATAGTCGTTA TATTGTTCAA TGGTTTTTAG ATTAGCATAG7860AAATTCACTT CCTCATCTGT CATCACAAAC AATTTCAATA GTGGGAGTTT AGTGCCATAC7920AATTCTATTT GCGAGTCCAT TTCTCTAATA CTCATCTTGA ACATGATGGG CTTACTGAAG7980AAAGGCAGAA ATATTCCAAG AATTGATCGA TCAAATTTAG AGAATAAGAA ATTAGGATTC8040TTTGCAATCG CATGCAACAG CTCCATAGTC TCACTCTCAA GCAATCGATA AACCTTTTTA8100AATTTATCAA CACCAATGCT TTTGATAACA GCGCGATAAT CTTTCATGTA ATTTCTTAGA8160ACCTTATCTA ACGCTCCCTT GTTTGTATGC ATCTGTTTGA AAAAATCATG CATGGTTTTT8220ATTTGATCTA TGATTTTCTT TTTTTCATCA GTGTTTAAAA GTTCGTTCTC CTGCAAACTC8280TCAATAAGTT TTTCTTTCTT ATCTTTTGAG TCAGCAATCA TTGAGAAGAG TTTTCGCATG8340TTATTACTCA TATCGCTCTT GGTCCCTTCA ATGGATTTGG TTTCTCTTTA GATTGTTCAA8400ATCGTAAAGT TTTATATTAA AATTATACAA TATCTGTTGT GTGAAAATTT CAGAGCGGTC8460ATAATTCAAA GAGCGAGAAA CTATTTTTTA ACCATAAAAC ATTTTTCAAT CACTCTTATA8520TCTGTTTTTC AAAACCTACA AAAAACGCTT TCACAAATAG CTCTAAAAAC CGATTTAAAA8580AAAGTTTTAA TGCTATAAAA AAGCAAGATA AGTCGTAAAA ATGCGTTACA ACTAAATAAA8640GGAATAACTC AATTTTCAAA AAGGAGTCTT AAGTTACCAT TATTACGATG TTAAACAAGT8700TCGTAATTAA AATTCTCAAG ACCAAATACA GAGAGATTAT TCAATATTTT TCAATGGATA8760TTGAGCTTTT CAAAATAGCA AAAATCAATA ATAAATCAAA CATCAATAAG ACTTTGTAGT8820ATTCTTTAAT TTGAACAAAT AAAATACAAA TTTTTTAGGG TATCGTAAAT TTTCTAAACT8880TTCTTTATTT TTAAAAACCC ATAGCAATCT TAATACCACT AGCTGAACAT TATGATTTGA8940TTGATGATTG ATTGATGATT GATTGATGAT TGATTGATGA TTGATTGATG ATTGATTTTT9000
GCTATTTTGA AAAGATCAAT ATCCATTGAA AAATATTATT ATAATCTCTT TATTAAATGA9060TAGGCTTGAT TATAGGCAAA AGGAGTGTTG TAATTTATTG GATTTCATAG AAGTATCTTA9120ACACTTTTTA GTGTAAATTG GTCTGTTGTT AAGAGTTGTG GGGACACTCC AATGTTTAGC9180AGAATACACT AAAAATTAAA TGGATGAGAT AGAACGATAC TAAAAATAGC CTAATGGGCT9240AGGCGCTTAC ATATGGTACT TTAAAGGACG GAGTTTTTCG CTAGTAAGAT AAAGTTATCA9300TTAGAGCTTG AGATACTTAC AAGAACCAGT TTTGCCATTG AGTCATAAGC ATGATTAGCT9360TCATTGTGAA GTTTGCGTGG CTTAAGAGAT AGTATTTGCT TATTATGCTG AGAGAAACGA9420GTAGTAAAAG ATAAGTAGTG TAACTAAAAA AGCTAGGTTT TATTATAAGA GCAAATAAGA9480ATAATATTGG ATAAACTAAA ATCACCCCTG CCCCATAAGA AAAAAGCCCT ATTAAAAAAC9540CTATGACGAT AGAGCTGATA TTGAACAGCC TATAATAAAG GCTGTACTTA TCTAAATGTT9600TGTTGAAAGA ATGTTTGAAT TGTAAGAAGT TTTGTTTTAA TTTGCTAATT GGCTGTTTCA9660TTTTGGTTTT TAAAGAAATA GTTCAAGGCG GTGAGCCTAT AAAGGAGCAT AAAATAATAA9720ATATTTTACA AAACCACCCT AATCTAACTC CAAATCTCAA AGAATACCCC ACTTGCCGAT9780ACTAACATGT GGTATAGCAC AAACCAACGA CTTGTTTGTT TATGCCAAAC AAAGAACAGA9840AAACATCTGT TAAAGAACAA AAGAGTTTTT CATTTAAAAA ATACCGACCC ATTTTATAAA9900AATAATCTTA AGAGAAACTT AAAAAATACC AACAAGCCGC ATACAAGTGA GAAAAACATA9960ACACTATAAG ACCTAGATTT TATTTACCTT TTGGATATGG AAAAATTTTG ATCCATAGTT10020TTGTAAAAAT TGTGGTAAAA TGTATTGATA TTTTTTGAAA TTTTAAGGTT ACAAAAACTA10080TAAGATGCTT GCAAAAATCG TTTTTGGCTC ATTGGTTGCG TTTGGAGTTT TGTCGGCTAA10140TGTGGAGCAG TTTGGTTCAT TTTTCAACGA GATAAAAAAA GAACAAGAAG AGGTGGCTGC10200AAAAGAAGAC GCTCTCAAGG CTAAAAAGAA GCTCTTAAAC AATACGCATG ATTTCTTAGA10260AGACTTGATT TTTAGAAAAC AAAAAATCAA AGAGCTTATG GATCATAGAG CTAAAGTTCT10320TTTAGATTTA GAAAACAAAT ACAAAAAAGA AAAAGAGGCT CTAGAGAAAG AGACAAGGGG10380TAAAATCCTC ACTGCTAAGT CAAAGGCTTA TGGTGATCTA GAGCAAGCCT TAAAAGATAA10440CCCTCTCTAT AAGAAACTTC TTCCTAACCC TTATGCCTAT GTTTTAAACC AAGAAACATT10500CACCAAAGAA GATAAAGAGC GTTTGAGTTA TTACTACCCC CAAGTGAAAA CGAGCAGTAT10560TTTTAAAAAA ACTACCGCTA CCACTAAAGA TAAGGCTCAG GCTTTGCTTC AAATGGGTGT10620GTTTTCTTTA GATGAAGAAC AAAACAAAAA AGCGAGCCGA TTAGCTTTAT CTTACAAGCA10680AGCGATTGAA GAATATTCCA ATAACATTTC TAATTTATTG AGCAGAAAAG AATTGGATAA10740TATAGATTAT TACTTACAGC TTGAAAGAAA CAAGTTTGAC TCCAAAGCAA AAGATATCGC10800TCAAAAAGCT ACTAACACGC TTATTTTTAA CTCGGAACGC TTGGCGTTTA GCATGGCGAT10860TGATAAGATC AATGAGAAAT ACTTAAGGGG CTATGAGGCT TTTTCTAACT TGTTGAAAAA10920TGTCAAAGAT GATGTGGAAT TGAATACTCT GACTAAAAAC TTTACCAATC AAAAATTGAG10980TTTCGCTCAA AAACAAAAAT TATGTTTGTT GGTTTTAGAC AGCTTCAATT TTGATACCCA11040ATCCAAAAAA TCTATATTAA AAAAGACTAA TGAATACAAT ATTTTCGTAG ATAGCGATCC11100TATGATGAGC GATAAGACAA CTATGCAAAA AGAACACTAC AAGATATTTA ATTTCTTCAA11160AACAGTGCTT TCTGCATACC GAAACAATGT TGCCAAGAAT AATCCCTTTG AATAGGAAAG11220GAGACACTCT TGAAAAGTAT CTTCAAAAAA CTAGGTTCTG TCGCTCTTTA TTTTTTAGTT11280ATTTATGGGG GCTTAAACGC TATCAATACA GCATTATTAC CGAGTGAATA CAAAAAATTA11340GTGGCTTTAG GCTTTAAAAA AATCAAAACA TTCCATCAAA GACATGACCG AAAAAGAAGT11400TACAGAAGAG GAAAAAGAAT TCGCCACTAA AGCTTTGAGA GAAAAATTAC GAAATGATAG11460GGCGAGAGCG GAGCAAATTC AAAAGAATAT TGAAGCGTTT GAAAAAAAGA ATAACTCTTC11520TATTCAAAAA AAAGCGGCTA AACACAAAGG ATTACAAGAA TTAAACGAAA CTAACGCTAA11580CCCTTTGAAT GACAACCCTA ATAGCAATTC TTCCACTGAA ACCAAATCCA ATAAAGATGA11640TAACTTTGAT GAGATGATCA ATAAGGTGAA TGAAGCTTTT GTGAAACCTG CTGCTCCGCT11700TGTGCCTAAT GAATGGAGAA CACCTGAAAT TGAAATCATT ATCAATGAGT GTATTATTTC11760
AAGCAACGAT TACGATGGGT TAAGAAAGTG TTTGATCAAA GACATCAAGG ATCAAAAAAT11820TCTTGCCCCC TTATTAGAAA AAATTCAAGA AATAGAGACA GAAAATAACA AGTTTTCTAG11880ACAACACTTG AGTGGTTTAA AACTCGCTCT TAATAACAGC AACAATAGAA CCTTTCTTAT11940AGCTTCATGC GCTATTTGTG AGAAGAGAAA AAAAGAAATG GAGCAAGAAA ACAACCACCA12000AGATACTACA AATGCAAGTG AGTTTGGAGC TACTGACACA AAAGAAAATG AAGCAAAAGA12060TGCAGCATTC TCAAACAATC GTTCTAAATC TGAACTGCCC AATAGCGTCA TTAATCAAAT12120AGAACAAAGC ATCGCTCATG GAAAAAAATA GCGATCAAAA TTATTAGCTC AAAAAAACAA12180CTAGAGAAAC AAATCCCAAA GGTTAGAAAT CATGGCCTGT CGTCTCAGAA AAATCATTTA12240ACAATAACCT TACTTGATTG CCTTTCTTGT AGGTATTGTC GCTTGCTTTG TTCTAGGGAT12300ATTTCTAATG CATCCAACTT CTCTAAATAA TTTAAAAAGA TCTTGTCGTG AGCTAACATG12360AGCTTTCTGA TTCCTTTGAT GAAATTTTTA TTCTTTAGGC TTTCTACAAG CGTCTGTGAA12420GCAGTGATTA AGGAAGCTGT ACCTCCAATA TTGCTCTGAT ACGCCTTTAG AGAAGTTTCT12480AAACGCTCTC TTATATTTGG TTTTTCTTGC TCGATTTTCA GCTTCCCTTC ACAATAAAGA12540ACTAAAACTT TATCGGATAT TCCGCATTGT TGCTCAGCAG TGTTTTGGTC TAAGGGATTG12600ATTTTCATAT AAGTTAATAA AAGTTCAGGG CTAGACATAT AAGTTTTGAA AATCACATCT12660TCTGAGATGA AAAATAACTC ATTCGCTTCA AAATTGGCTT TCAATAACGC TAAATCTCCT12720CTCAAAGCAA TGGCCGCTTT TTTGATGTTT AGAGCATCTT CTTGACCTAT TTCATTATTA12780GCGCTAGGGC TAGTGGTTGA AAAAATCTCA TCTAGGTTTT TGAGCACTTG TTGGTTGGTC12840TCTTTGTAGG TGCTATCAAG TTGCTTTAAA CCGCTTGTTA TATCTTCTGC TATCAAAACA12900GACAATAGCA AAAAAGAAGA TATGGTATTT TTCACGAGTG TTTTCATTTG ACAATAACTT12960TAGAGCTAGC AATGTTTCTT GCTGTCGTTT CTCTCTCTAA TTTCAGTTGT TCTTCCCAAA13020GGTCGGCTTT TTTTTCAAGA TTCTCTATAT AGTTTAAATG ATTTTCTGCG TTTAAGATAG13080CAACTTCTAT GAGCGCATTC AAATCTACTG ATCCTTTTAA GGTTTTGATT TCTTCATTGA13140TCCCATTCAA ATAAGCGATA TTTTGAAAAT CTGCATCATT TAGTTTATTT TGAATAAGGG13200CTACAATCTT TCTGTAATTC TGAATAACCT GTTCCATAAG GCATGCTGAA ATTTTTAGCC13260CATCAAGATA GGGGCATTTT GTGGGTGCTA GAGTAAATGT TTCAACGATT CCAAATGGTG13320CACCCATGCT TGAAAAAAAA CTAAGAGCAG GCGCGTAGAT GGTGCTTTGA AACAAATCCT13380GACCTGTTAG GGAATTGTAG TCAATAAGGG TCGCTTTTTG CATAGCCGCT TTCAACCATG13440TTTCAAAACC TTTTAAGGTT TCTTCAAACG CCTTGATACC AATCGTATTG TAAGCGATGT13500ATTTAGCGTT GTCAGAAGAA CTTCCTAGAG CTTGAGAAAG CATTTCCATT TGTGTTTTTA13560GGGTAACGCT CGGTTCAAAG CTGTTTTTTA ACGCTTCTAA GAGAGCGTTT TGCTGGTTCA13620TTTTGAGCTT GATTATTTCG TTATTTTTTT GGAGCGCGAT TTGCATGTTT TGGATTTCTG13680TTTGGGTATT AATTTTTTGT TTTTCCACGA TCATGTTGAC ATTCCCTCCC AATGCGCTAA13740GCGCGCTTGA ATACCCTTCC ATGATGCCAA GCAAGATGTC TGAACCTGCA AAAAACCCCC13800CTGTCATGCT ATTGACACCA TTAATAACGC CATTAGCCCC TTTTAACATA GCACTTATAG13860TTGCAAGCTG AGTTCTCAAT TGTCCCTCTA TTTGCGCTTG AATGGCTTTT TCTTTGGCAC13920TAGATTGAGC CTCTATGGCT TTTAATTCGG CTTGAGCGGT TTTTTGTTTG GCTTGTGCAT13980CCGCCTGAAT GGCTTTTAAG GCAGATTCAA GCGTTATTAC TGCCCCTGCA CCATTCAGAA14040ACAAACCACA AAAAGTTAAG AAAGAAAATA TGCTTAAAAA ACATTTCACA TCTCTTTCCT14100CACTTCACGA TTATTTTAGT TTGCACCCTT TCTGTTAAGT AGCTATCTTT TTGCCCCTTA14160AGTTTATCTT TGATGTAATC AAGATAAGTC AAATGCGATT TCAAAAAAGA TTTATTCGCT14220ACTATATTGT AATTATATAG CGAGCTTATG TTAGAAATCG CTTGAGTGTC ATAGGTGCTA14280GTAGCTAATC CTGATTGATT AAGTATCATT TGAGAAGCGT TTTGCAACAA ATTGGTATTG14340TTTTTCACAA ATTCTATATA GTATTCTCTC AAAATTTCTG CTACTTTTTC AGCATAGCAA14400TAAACGGCAA GAACTTTGTC CCCAATAGGG CATGCAGGAG TGGTCATAGG ATTAACGCCT14460GAAGTTAGGG CATTAGTGGC CAGTGCTTGG TATTTGGCAT AAACAGTGGG CATAGAGACA14520
CTCATAGGAG TCATAGAAAT TTGCATGCAA CTAAAAAACA CTTTTGATGA GCCAACAAGT14580GCACCTAAAG CGGTACAGCT ATCAAGGGAA TCGGCTGTAT CATTCATTGA GCTGTTGCTT14640GCTTGAGAAC GCAGTTGCTC TTGTAGAGCT AGGGCGTATT TTGGTGCTGC ACTTGTAATA14700TTGCCTAAGA TACCGCTCAT CATTTCAACC GTTGTTGGCA CACTAGGAAC AGCGATTTGA14760TTCGTCGCAT AAGCTTCAAT AGCACTAGGA TTTTTAGGGG TCGTGTTACT CGCTAAAATG14820CTTGCAATCT GACTATTAAC AGCACTAATT TGCGCGCCTT GCGTGTTGCC TTGCGCGTTA14880AATTCCCCTG TTAATTTGCT GATATTTAAG ATATTGTTCC CCACAGCCAT GCTTTGATCG14940TTAAAACCTT GATACAATTG GTTGTATTGT TGGTTAGCGG CTTTCATAGG CATGCTTACG15000GCTTCAGCGA TGCTTTGATT GTATTGGGTC ATGATAGCGG TCATTTGCGG ATTCGTAAAT15060CCCACAACAA TAGGAATAAT CGCTGCTGAC ATAGCACCCG CTACTATTCC TGCAAATGGC15120CCTGCGACAC CACTCGTGCT TGAGATGATT GAGGAAATTT CCGATAAGAA GCCTGCAGAA15180GATGATTCAT ATATAGCTTG TGTGCCTGCC ATGTTAAAAC CCCCCTAAGC TTGGTGGTAA15240AAACGATGAA TCTGGATATG TTGGTGCATA ACCATACATG AAAGGATTGT TTGGGCCGTA15300ATCGCCCATC ATTTGGCTCA TGAGAAGATT TTGAATGCCC CACATCGCAT TGATACCTAG15360ATTATCATTA GGTTGAAAAC TCCCTAAACT TATGTTGTCA AACTTGATAT TAACATTCTT15420ATCATTATAG TCATTGAGTA TGGCCACTTT TTGCTCTAGG GTTTCTTTAG GGATCTCTAT15480TTTTAGTTGA TCTCTAGAAA CAAGCCCCAC GCTATTTAGC GCCATATCTT CAGGACTAAT15540ATCTTTTATA TCAGTGTTTT GGTCAGCGTT AAGCGGAGCG TTAAACATGC CAATGATAAG15600ACACCAAGCA AATAGTAATT TAATTTTATA AAAATTCGTT TTCATACTTT TGACTCCTTT15660ATTCTTATTT TTAGCACTAT TCTAGCGCAT TAACACCACT CAATCGCTGT TTTTGTTTTG15720ATTTTTTTGA TCGAGCATTT TGTTTGTTAC TTCATCAATG TTTTGAAAAT ATTTTTCAAA15780AAGCTCTTTC TTTTTAGCTT CAACGCTCAT ATCAACCTGA ATCCAATTAG GAACAATGGA15840GTCCATGATT AAATGCGTGA AGTCATAGGC ATGATTGGTT GGGTATATTT TGTTTTGAAC15900ATAGTATTCT AAAAAATTCG CTTGAACAAA AAAATTCTCT ATATCGCTCT GCATATCCTC15960GCTTATGTTG TTATTGATAG GTTTTTCTAG TAATCTGAGA ATCCTATACG ACTGCATGAT16020AGTTTCTAGC ATGAAGTAAA CATAAACATA AACTAATGAG ACAAAAGAAT TATTTGCTTT16080AAACGAGCTT GCGTCATTTT TCCCACTTTG AAGAGGAATT AATCTTGATA GTGTTTTTTG16140GGGATCTTGC CCATCGTTTA TTTCTTTAAA AAAGCGGAAG TCTAAATCCT GATTATTGAG16200AAATGACTTG ACAAAGTGAA GATTAGCATT GAGACTATCT ATGAGACCTG AATAAAGGTG16260TTCTGTTTTG ACATCATCTA TATTTAAAAC ATTCTCATAA AACACATTGA CATGGTCTTC16320TAAGAAATTA GAAAAATCAT AAAGAGCGGT AAGGTTTTGT TCAGTGATTT CGCCTTCCAT16380TTCTTCTTCT ATGAAGTCCA ATTCTTCTCT CAGTTCAAAA AGATAATTAG AAAAACTATC16440CAAAATCGTC AAGACATCAT TTTCTAAAAT TTTCAATAAT TTTTGTTCAC GCAAACTTTG16500TTTCATTTTA ATACTCCTTT ATTTGTTGAT ACATCTGCCT CAAGGCCTGA TATTTATCTA16560TGATACTATG GTTTTGAATA ATCTTGTCAA TTTCTTTGAC AAATACAGTA TCTGTGGATA16620AAATTTTCAA ATATTCTTTA GGAATGCCTC TCAAATTAAA ACTAGCGATA ACGCTAGGGC16680TTCCATCTTG TTTGTAGAGA ATTTTCCTAT CTAACCCCTT AGTGATGATT TCAAATCTTT16740TTCTGTAACA TGGCTATCTT TGGTAACAGA AAGATTGCCC CCATCGTTTC TCAAAAAAAT16800CTTTGTAGGG CATTGTTCTC TAATAGTATC AGCCATAGGG CAAGCCAAAA GATCAGTGAT16860GCTTTGAGTC GCAAGCCTGA CAATAGCGTT TCTTTTCCTT GCAGTTTTTA GCATGTCTCT16920TACAAAATAA GCAACCTTTG GATCGCCTAA ATATTTCCAA GCTTCATCAA TATCTAAAAC16980AAATCTACGC CCATCCATTG CCTCTTGGAT ACGAGCGAAA AGGTAAAAAC AAATAAAGGG17040CGAAACATCA TTATTGTCTA GGAAACTTGA CCCATCAACG CCAATAATCG TTTTTGAAAA17100ATCTAAGCGA TCGGTTGCTT GATTATCAAA AAGCCATTGA AATTCACCAT TGGTTGATTT17160GCAAAAAGGC GCTAATCGCG CGACAAGCCC ATTAGGATCA TTGTGGTCTT TTCCGAAAGC17220ATTAATCAGT TGGGTGATGG GATAATCTAG GTTCATATCT CCTGTGATAA GGTTGGTTAC17280
TGCCGCTGCA AGCGTATTAG AATCAGCTAG GCTAAAAGTG GTGCTGTTGC CATTTTCATC17340TTTTTCATCG CTTTTGGTTG CTAAGTTTTT CACAAGTTCT TTGACAACAG AAATAGCTGT17400TTGTTTTTGC TCCATTGTTG CATTGGATTT TTGCACACAA GCCGCCCAAG CAAAAGGATT17460TAATCCTGTA TCTGTCCCTA GCTCAATCTT GACATACTCC CCACCCATTG CGACAATATT17520CCCATAAGCG CCATAATCTT TATCCATATA GACCATAGTG AGCTTTTGTC TGTCTTTGCT17580GACATTAGCA GGAAAATTGT GAGCAAATTG TCCCATAGCG TTTAGAGTCA TTGACATAAA17640CACCGTCTTA CCTGAACCGG TTGAGCCAAG TATCAAAGTG TGTCCTGCTG AAGCCGAACC17700AAAATCAGTG GGCATGTGGA AGTTCAGATA AAAAGGCGAA TCGATCTCGC TTTTTAGCGT17760CATGACGCTA TTACCCCAAG CGTTATTCTC TTGATTGCCA TCAAAACTCA TCGCTCTCAT17820AGCGATAAAA TCAGCAAAAT TATTAGAAGT TACATCAAAA ATAAAAGGAA GCGTGATAAA17880AGAGCAATGT TTGGCAAAAA AGTAATTTTC CATAGAGAAA GTCGCTGCGT TGGCTAAAAA17940ACCTTTAGCG TTAAGACTAG AGACGCATTC CTTGACGCTT TGTTTCATTT TTTCAAAGCT18000ATCAGCAAAC AGCACTAAAG AATTACCATA ACTGCCTAGC GTAATATCAC CATTACCCAC18060TAATTCACTC AAGCAACCTA AAGTCATGCC CTGCTCTTTA GAACCCCCAC TAATAATAAT18120CCTTCTAGAG GTGAAAGCTA GTTTGTCCTT TAAAACCTGT GAGTTTTTAG GCGAATAAGC18180ATGCATAAAG ATGAACTCGC TGTCTAGAGC GTTGATTTTA TCAAACAAAT CGCTCTGTGA18240TTTAGGGGCG TATTCACTAA TCTCAATAGC GCTAAAATAC TTTTCACTCA AATCGTCATT18300TAAGATTTTT CCATGCTTAT TGGCAAAATA AACTTCTTTC ACCCCACCAT GCATTTTTTC18360CTTGAGATAC AAGTCTTTTC TGTTGCAAAT AAAAGGGGCT TCATTCATTC CCACAAGAAA18420ATTGTAAAAC TCGCATTGTT TGGAATAAAC AACGCCATCT TTGGTGTATT CTTTTAATCT18480AGTGGGGTGG TATTTACTCA ATAGCTCTTC TATGAGCTCT ATCCTATCCT TGAAGTTTTC18540AAGCTTGGCT CTAATAATCC TTTGAAACTC TTCAAAATTA TTGTCTGCAA AATGCTTTTT18600ATTCATAACA GGTTCATTGA GAGTGTCTAA TAAATCTTGC TCTATGGTCA GAAAAAAACT18660AATATCATAA AAACTTTCTC TCTTTTGCTT CTCATTATAG GCCCGCATGA AATCATTAGA18720AAAAATAAGA CTATAGTCCC TATTGGTTTC ATCAATAACG ATTTTCTTTT TAACAGTGTG18780AAAATAAAAT TTGAATTCAG GGGTAACAAA ATTCCTAAAA ACGCTATAAA TAGAAGCGTG18840TAATTCTATG AGATCTTTTT TGGAAGTGGT TAAAAAATCA ATGCCCCCCA ATTTGATTGT18900GCCTAAAAGA GAATAGTTGT TAGTAAGGAT CACCCCATCA TCTAAAAAAC ATTCATAGTT18960ATTCGCTAGA TAGGAGTTTG CAGCACTCAC AAGTCTGTCT TCTCTGTTTG GATTTAAGTG19020GATGTCATTA GCCATTTCTT TACTAGGCTT CATGGAAAAA ATGCTCATGA ATGCTTTGTT19080TTTCACGCCC TTAAACAAAA AAGGTTTTTT AAATTTCATC GCTCGCTCCA TTCTTTGATA19140AAGCCTATAG TCTTTCTTGA ATCCAAGAGC TACAAGCACA ATAACAATCG CCACAATCAA19200AACAGGTTCA TAGGCTTGAA AAAGAATAAC AGATAATACA ATTGTTACAA ACAATATAAA19260TATAGAGGAA TAAATAAAAG TTTCAGGGAA ACCAAACAAC CTATTCCCCC CATCAAACAA19320GACTTTAAAA AAGGGGTTGA CACCTTTTTG CATGTCTGCT TTAAGCTCTT CTATTTTTTG19380AAACTGCCGC TTTTGAACCT CTTGCTCTAT GATTAGCTTT TTTTGTTCGT CAGCTTGCCT19440GCTTGCCACA AACACCCCTC CCTTTATAGG TATACCGCTT CACATGTAAT CGTATAAAAG19500ATTTTTTTGA GAGACTCTAT AGCGCTAATA TGTTTCAAAA GATCATTAGG GTCATAAGAA19560TTGAATACGG CCAATAAAAC ATTATACAGC TTATCATCGC ATAGAATTTC TCTTGTTTCC19620CCGTGCAACG ATAGGAAGCA GCGTTGTTTG TTGGTCGTGC TGATGCTTTT GAAAGTAAAA19680AAGTCTTTCA CTTCAGGATT GATCTGCAAT TCCACATTCA ACCCCATTTC TTTACCCTTT19740TCATCAAAGA TTTTTTCAAT AATTGGATCG TAATGCTTCA AATCCTTTAT TTTTTTAAGG19800ACTCTATTGA CAATCACGAA ATCAAAAACT TCATTTTTGA TAATATCGGG ATTGATCTCT19860TTGAAAGTTA CTTTCTTGTC TTGCAAATTT TTGATAGTCG CCTTGAAACT GTCAAAATCT19920AAATTCGTAT AAACACGCCC ACTAGGAGTG TTTTTTTCTT TTTCTAGCGC TTCTTTTTTC19980GCTTGATTAT CATCATTTGC CAACCCACAC GAACTGAAAA CAACGAGACT TAAGAGAACT20040
TTCAAAAAAA AGTCTCTTAT TTTTTTATTA CTATTATTGT TGATCAATTT AGCTAGCTCC20100TCCACCTTCG CCAATATTGA AGCCAAACTT AGTGCTTAAA TAGATAATAC CGCCTGCCAC20160CGCTAACATA GCTATGGGTT GCGCATAAGC AAAAACAGTT GCCTGACCTC TTTTGATGTC20220ATCAGAGATT TTCCAAATAT CTGCTATGCC TTTGACCCCT AGAGCGCAAC CACCTACGAT20280CGCTAGAACA GAAATGATCT GAACAACCAA AGTTTTAGTC TCAGTAACGC CTGTTGCAGG20340ACTGTTGAAC GCCATTAAAG GATTGGTTGT TATCGCTAGC CCTAAAGTTA CTACAACTTT20400CTTGTAGCTG TCAGTGATTC TTGTAAGAAA TTTCATGCGT TTCCTTTCAA ATTGAAATCA20460ATCGCTTGAG TATATCAAAA AAAAAGTATT TTTATACCAT TCATACAAGC ACTACTTCAT20520AATTTAAATC AAAACCGACA CTTTTGTTTG ACAATTGGCA CAATTTAGAA CAATAAACCT20580ACTTGTCCCA ACCTTTTTTG TTTCTCAAGT CGTTGTAGAA TTGTAGATCT TTAGGATCTT20640TGATGTATTT TTTAATCGTC TCAGGTTGAA ACCTAAAAAC AAGCAAAAAC AAACCCAAGC20700TGATCAAAGT GAGAATAAAG CTCCATTTTA AGCAACTCCA TAGACCACTA AAGAAACTTT20760TTTTGAAGCT GTCTTTGAAA ATCTGTCCTA TTGATTTATT TTCCATGTGG ATCGCAAACA20820CTTCTTGTTA AAATTTGTCT ATTTTAGCGA GTTTTTGTCA ATTATAGGTA AAAATGTGAA20880TCGTTCCTAG CCTTTAGACG CTTGCAACGA CTGGAATTTT TCAATATTAA CAATATTAAT20940GAAAAAATGC CTAATATTGT GGTATAATAA GAATGTTCAA AGACATCAAT TGACCACTCA21000AGCGTGTGGT GGTTTTTAGC AGTCTTTGAT ACCAAATTAG GTTAGTAAGG AGAAACAATG21060ACTAACGAAA CCATCGATCA AACAACAACA CCAGATCAAA CAGATTTTGT TCCGCAACGA21120TTTATCAATA ATCTTCAAGT AGCTTTTCTT AAAGTTGATA ACGCTGTCGC TTCATTTGAT21180CCCGATCAAA AACCAATCGT TGATAAGAAT GATAGGGATA ATAGGCAAGC TTTTGAGAAA21240ATCTCGCAAC TAAGGGAAGA ATACGCCAAT AAAGCGATCA AAAATCCTGC CAAAAAGAAT21300CAGTATTTTT TTAGACTTTA TCAATAAGAG CAATGATTTA ATCAACAAAG ACAATCTCAT21360TGCTGTAGAT TCTTCCGTAG ATAGCTTTAA GAAATTTGGG GATCAGCGTT ACCAAATTTT21420TACGAGTTGG GTGTCCCTTC AAAAAGATCC GTCTAAAATC AACACCCAAC AAATTCCGAA21480ATTTTATGGA AAATATCATA CAACCCCCTA TCTCTGATGA TAAGGAAAAA GCGGAGTTTT21540TGAGGTCTGC CAAACAATCT TTTGCAGGAA TTATCATAGG GAACCAAATC CGATCGGATC21600AAAAGTTCAT GGGCGTGTTT GATGAATCTT TGAAAGAAAG GCAAGAAGCA GAAAAAAATG21660CAGAGCCTGC TGGTGGGGAT TGGCTTGATA TTTTTTTATC ATTTGTATTC AATAAAAAAC21720AATCTTCCGA TCTCAAAGAA ACGCTCAATC AAGAGCCAAG GCCTGATTTT GAACAAAATT21780TAGCCACTAC CACCACTGAC ATACAAGGCT TACCGCCTGA AGCTAGAGAT TTGCTTGATG21840AAAGGGGTAA TTTTTTTAAA TTCACTCTTG GTGATGTGGA GATGTTGGAT GTTGAGGGAG21900TCGCTGACAA GGATCCCAAT TATAAGTTCA ATCAATTGTT GATCCACAAT AACGCTCTAT21960CTTCTGTGCT AATGGGGGGT CATAGTAACA TAGAACCTGA AAAAGTTTCA TTATTGTATG22020GGGATAATGG TGGTCTTGAA GCTAGGCATG ATTGGAACGC CACCGTTGGT TATAAAAACC22080AACAAGGCAG TAATGCGGCC ACACTCATTA ATGCGCATCT TAATAACGGC AGCGGGTTAA22140TCATAGCGGG TAATGAGGAT GGGATTAAAA ACCCTAGCTT CTATCTCTAC AAAGAAGAGC22200AACTCACAGG CTTGAAACAA GCGTTGAGTC AAGAAGAGAT CCAAAACAAA GTGGATTTCA22260TGGAATTTCT CGCACAAAAC AATGCTAAAT TAGACAACTT GAGCAAGGAA GAGAAAGAAA22320AATTCCAAAC TGAGATTGGA AATTTCCAAA AAGACCGTAA GGCTTATTTG GACGCTCTAG22380GGAATGATCA CATTGCTTTT GTTTCTAAAA AAGACCCAAA ACATTTAGCT TTGGTTACTG22440AGTTTAGTAA TGGGGAATTG AGCTATACTC TCAAAGATTA TGGGAAAAAA CAAGATAAAG22500CTTTAGATGG GGAGACAAAA ACCACTCTTC AAGGTAGCCT AAAATATGAT GGCGTGATGT22560TTGTCAATTA TTCCAATTTC AAATACACCA ACGCCTCCAA GAGTCCTGAT AAGGGCGTGG22620GCACTACGAA TGGCGTTTCC CATTTGGAAG CAAATTTTAG CAAGGTAGCT GTCTTTAATT22680TGCCTAATTT AAATAATCTC GCTATCACTA ATTATATAAG GCGAGATTTA GAAGATAAAT22740TGTTGGCTAA AGGATTGTCC CCACAAGAAG CTAATAAGCT CATCAAAGAC TTTTTGAACA22800
GCAACAAAGA ATTGGTTGGA AAAGTTTCAA ACTTCAATAA AGCTGTAGCT GAAGCTAAAA22860ACACAGGCAA TTATGACGAA GTGAAAAAAG CTCAGAAAGA TCTTGAAAAA TCTCTAAGGA22920AACGAGAGCA TTTAGAGAAA GAAGTAGCGA AAAAATTGGA GAGCAGAAAC GACAACAAAA22980ATAGAATGGA AGCAAAAGCT CAAGCTAACA GCCAAAAAGA TAAGATTTTT GCACTTATCA23040ATAAAGAGGC TAGTAAGGAA GCAAGAGCGG CCGCTTTCGA TCCGAATCTT AAAGGCATCA23100GGAGCGAATT GTCTGATAAA CTTGAAAATA TCAACAAGAA TTTGAAAGAC TTTGGCAAAT23160CTTTTGATGA ACTCAAAAAT GGCAAAAATA ATGATTTCAG CAAGGCAGAA GAAACGCTAA23220AAGCCCTTAA AGACTCGGTG AAAGATTTAG GCATCAATCC AGAATGGATT TCAAAAATTG23280AAAACCTTAA TGCAGCTTTG AATGATTTCA AAAATGGCAA AAATAAGGAT TTCAGCAAGG23340TAACACAGGC AAAAAGCGAC CTTGAAAATT CCATTAAGGA TGTGATCATC AATCAAAAGA23400TAACGGATAA AGTTGACAAT CTCAATCAGG CGGTATCAGA GACTAAATTA ACAGGCAATT23460TCAGTAAGGT AGAGCAAGCT CTAGCCGAAT TCAAAAGCTT GTCATTGGAT CTTGGAAAAA23520ATTCTGATCT ACAATCCGTT AAAAATAGTA TAAATGGAAC CCTAGTCGGT AATGGGTTGT23580CTAAAACAGA AGCCACAACG CTCACCAAAA ATTTTTCGGA CATTAGGAAA GAATTGAACG23640AGAAATTATT TGGAAATTCC AATAACAATA ATAATGGACT CAAGAACAAC ACAGAGCCCA23700TTTATGCTCA AGTTAATAAA AAGAAAGCAG GACAAGTAGC TAGCCCTGAA GAGCCCATTT23760ACGCTCCAAG TTGCTAAAAA GGTGAGTGCA AAAATTGACC AACTCAACAA AGCTACATCA23820GCAATAAATA GAAAAATTGA CCGGATTAAC AAAATTGCAT CAGCAGGTAA AGGAGTGGGC23880GGTTTCAGTG GAACAGGGCG ATCAGCTAGC CCTGAACCCA TTTACGCTAC AATTGATTTT23940GATGAGGCAA ATCAAGCAGG CTTCCCTCTT AGGAGAAGTG CTGCAGTTAA TGATCTCAGT24000AAAGTAGGGC TTTCAAGGGA GCAAGAATTG ACTCGTAGAA TTGGCGATCT CAATCAGGCG24060GTATCAGAAG CTAAAACAGG TCATTTTGGC AAACTAGAAC AAAAGATAGA TGAACTCAAA24120GATTCTACGA AAAAGAATGC TTTGAAGTTA TGGGTTGAAA GCGCGAAACA AGTGCCTACT24180AGTTTGCAGG CGAAATTGGA CAATTATGCT ACTAACAGCC ACACACGCAT TAATAGCAAT24240GTCCAAAATG GAGGAATTAA TGAGAAAGCG ACCGGTATGC TAACGCAAAA AAACCCTGAG24300TGGCTCAAGC TCGTGAATGA TAAGATAGTT GCGCATAATG TGGGAAGTGC TCATTTGTCA24360GAGTATGATA AAATTGGATT CAACCAAAAG AATATGAAAG ATTA 2440權(quán)利要求
1.一種幽門螺桿菌致病島疾病基因檢測芯片，其特征在于所述的芯片上固定設(shè)置有探針，所述的探針包含SEQ ID NO1-26中85％以上保守序列的PCR產(chǎn)物，所述的PCR產(chǎn)物是以國際標(biāo)準(zhǔn)菌株HP26695序列為參考序列設(shè)計(jì)特異性引物，以臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到的。
2.如權(quán)利要求1所述的一種幽門螺桿菌致病島疾病基因檢測芯片，其特征在于所述的芯片上還固定設(shè)置有SEQ ID NO27-40中的cag致病島基因特異性寡核苷酸。
3.如權(quán)利要求1所述的一種幽門螺桿菌致病島疾病基因檢測芯片，其特征在于所述的PCR產(chǎn)物以國際標(biāo)準(zhǔn)菌株J99的序列為參考序列設(shè)計(jì)特異性引物。
4.權(quán)利要求1所述的幽門螺桿菌致病島疾病基因檢測芯片的制作方法，其特征在于，包括在H.pylori感染患者中選取代表性菌株，測定其全基因組序列；與已知的標(biāo)準(zhǔn)菌株HP26695序列相比較，找出DNA序列多態(tài)性特征；參照保守序列設(shè)計(jì)PCR引物，將臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到產(chǎn)物作為DNA基因芯片的探針之一；對于基因內(nèi)部多態(tài)性明顯、且保守序列較短者，增加設(shè)計(jì)保守序列開放閱讀框片段對應(yīng)的寡核苷酸片段；按照DNA基因芯片的制作工藝制備DNA基因芯片。
5.根據(jù)權(quán)利要求4所述的幽門螺桿菌致病島疾病基因檢測芯片的制作方法，其特征在于，包括下列步驟a)在H.pylori感染患者中選取胃癌患者、十二指腸潰瘍患者或慢性胃炎患者，獲取代表性菌株，并測序獲得其全基因組序列；b)與已知的標(biāo)準(zhǔn)菌株HP26695序列相比較，并將不同代表菌株cag致病島基因序列相比較，尋找特異性的DNA多態(tài)性特征；c)參照保守序列設(shè)計(jì)PCR引物，將臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到產(chǎn)物作為DNA基因芯片的探針之一；對于基因內(nèi)部多態(tài)性明顯、且保守序列較短者，增加設(shè)計(jì)保守序列開放閱讀框片段對應(yīng)的寡核苷酸片段，合成該片段作為額外探針；d)按照DNA基因芯片的制作工藝，將探針經(jīng)點(diǎn)樣儀點(diǎn)印在經(jīng)預(yù)處理的玻片上，經(jīng)固定工藝，制成成品DNA基因芯片。
6.如權(quán)利要求4或5所述的幽門螺桿菌致病島疾病基因檢測芯片的制作方法，其特征在于與已知的標(biāo)準(zhǔn)菌株J99的序列相比較，找出DNA序列多態(tài)性特征。
7.權(quán)利要求1所述的幽門螺桿菌致病島疾病基因檢測芯片的質(zhì)量控制和檢測效率驗(yàn)證方法，其特征在于，包括下列步驟a)來源于待測幽門螺桿菌基因組DNA的等量待測DNA標(biāo)本經(jīng)隨機(jī)九聚體Cy3-dCTP或Cy5-dCTP熒光標(biāo)記原料的逆轉(zhuǎn)錄獲得不同熒光標(biāo)記的雜交樣本；b)樣本純化；c)芯片雜交實(shí)驗(yàn)；d)經(jīng)計(jì)算機(jī)熒光掃描獲取雜交信號數(shù)據(jù)；e)熒光信號數(shù)據(jù)經(jīng)標(biāo)準(zhǔn)化處理，計(jì)算Cy5/Cy3的Ratio值，所有的雜交點(diǎn)對應(yīng)的Ratio值均在0.5-2.0之間為合格。
8.權(quán)利要求1所述的幽門螺桿菌致病島疾病基因檢測芯片的使用方法，其特征在于，包括下列步驟a)胃鏡檢查獲取的幽門螺桿菌感染者胃黏膜組織標(biāo)本經(jīng)分離、培養(yǎng)48小時(shí)，得到待測菌株，并經(jīng)DNA分離純化操作獲得待測菌株的基因組DNA；b)相關(guān)上述方法獲得待測熒光標(biāo)記樣本(標(biāo)準(zhǔn)菌株DNA以Cy3熒光標(biāo)記，待測菌株DNA以Cy5標(biāo)記)；c)進(jìn)行芯片雜交實(shí)驗(yàn)，并進(jìn)行數(shù)據(jù)分析。
全文摘要
本發(fā)明屬于臨床醫(yī)學(xué)應(yīng)用領(lǐng)域，公開了一種幽門螺桿菌致病島疾病基因檢測芯片，所述的芯片上固定設(shè)置有探針，所述的探針包含SEQ IDNO1－26中85％以上保守序列的PCR產(chǎn)物，所述的PCR產(chǎn)物是以國際標(biāo)準(zhǔn)菌株HP26695(或J99)為參考序列設(shè)計(jì)特異性引物，以臨床分離的中國人群疾病特異性幽門螺桿菌菌株提取的基因組DNA作為模板，經(jīng)PCR反應(yīng)得到的產(chǎn)物。本發(fā)明還公開了一種幽門螺桿菌cag致病島疾病特異性基因檢測芯片的制備方法。本發(fā)明克服了傳統(tǒng)分子生物學(xué)技術(shù)繁重的工作量和低效率，用于檢測臨床患者(宿主)感染幽門螺桿菌菌株cag致病島疾病特異性基因型。了解這些基因型，對預(yù)后不同感染結(jié)局、制定治療和隨防策略提供指導(dǎo)。
文檔編號C12Q1/04GK1800413SQ20051002311
公開日2006年7月12日 申請日期2005年1月4日 優(yōu)先權(quán)日2005年1月4日
發(fā)明者蕭樹東, 陳勝良, 曹芝君 申請人:上海第二醫(yī)科大學(xué)附屬仁濟(jì)醫(yī)院